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SSRN 2023年

作者： Aurum, Fawzan Sigma Zaman, Muhammad Zukhrufuz Purwanto, Edi Praseptiangga, Danar Nakano, Kohei Doctoral Program of Agricultural Science Faculty of Agriculture Universitas Sebelas Maret Jl. Ir. Sutami 36 A Kentingan Central Java Surakarta57126 Indonesia The United Graduate School of Agricultural Science Gifu University 1-1 Yanagido Gifu501-1193 Japan Research Center for Food Technology and Processing National Research and Innovation Agency Jl. Jogja – Wonosari Km 31.5 Yogyakarta55861 Indonesia Department of Food Science and Technology Faculty of Agriculture Universitas Sebelas Maret Jl. Ir. Sutami 36 A Kentingan Central Java Surakarta57126 Indonesia

Coffee is an export commodity that is prone to fraudulent practices. Therefore, this study presents a novel approach to authenticate coffee origins using targeted metabolomics with gas chromatography-mass spectrometry (GC-MS) and machine learning models. A total of 200 coffee samples from different harvest years and areas from Indonesia were extracted using the derivatisation method and then analysed for their metabolite profiles. Several supervised machine-learning models were tested to classify coffee origins and discover their potential markers. The study found various metabolite features spanning diverse chemical classes, encompassing sugar alcohols, carbohydrates, amino acids, organic acids, fatty acids, and phenols. Random forest (RF) and partial least squares discriminant analysis (PLS-DA) were among the most accurate models in predicting the origin of coffee from several classes in the validation dataset. The accuracy of both models is in the range of 91% to 100%. Furthermore, this study proposes a new strategy for determining "intersection features" as the set of features that are important and common to both RF and PLS-DA models, thereby providing a robust selection of coffee origin markers. Overall, the approach and findings of this study have far-reaching implications for coffee authentication. © 2023, The Authors. All rights reserved.

关键词： Mass spectrometry

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Arrowroot, cassava, and sweet potato starches combined with pectin: New materials for electrospinning of biopolymers

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food Hydrocolloids 2025年

作者： Igor Henrique de Lima Costa Gabriela de Oliveira Karine Laste Macagnan Angelita da Silveira Moreira Elessandra da Rosa Zavareze Alvaro Renato Guerra Dias Laboratory of Biopolymers and Nanotechnology in Food (BioNano) Graduate Program in Food Science and Technology Department of Agroindustrial Science and Technology Federal University of Pelotas 96010-900 Pelotas RS Brazil Technological Development Center Biotechnology Federal University of Pelotas 96010-900 Pelotas RS Brazil

The main objective of the study was to investigate the interaction of starch and low-methoxylated pectin in the elaboration of electrospun fibers, verifying the hypothesis that the interaction of this polymer with starches from different botanical sources (arrowroot, cassava and sweet potato) allows the development of electrospun materials with distinct characteristics, using multivariate analysis methods to prove the hypothesis. The development of electrospun materials with pectin (0, 2, 6 and 10%) allowed the production of nanometric and ultrafine fibers with diameters ranging from 156 to 465 nm for arrowroot starch fibers, 192 to 612 nm for cassava starch fibers and 136 to 446 nm for sweet potato starch fibers containing 0 and 10% pectin, respectively. Infrared spectra indicated the formation of amorphous regions in the fibers, which were confirmed by X-ray diffraction. There was a loss of thermal stability and surface modifications with increasing pectin concentration. Principal Component Analysis indicated that the degree of esterification and the increase in pectin concentrations increased the average diameters of the materials. Hierarchical Cluster Analysis showed that three distinct fibers were obtained. Therefore, electrospun fibers of starch and low methyl esterified pectin were obtained with distinct characteristics dependent on the starch source.

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Solar-Powered Desalination: Advancements in technology and Predictive Modeling for Sustainable Water Production

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SSRN 2024年

作者： Lisboa, Halana Santos Nascimento, Victor Ruan Silva da Silva, Alan Rozendo Campos de Resende, Iraí Tadeu Ferreira Bharagava, Ram Naresh Saratale, Rijuta Ganesh Saratale, Ganesh Dattatraya de Souza, Ranyere Lucena Figueiredo, Renan Tavares Ferreira, Luiz Fernando Romanholo Catalysis Energy and Materials Laboratory Institute of Technology and Research Tiradentes University Av. Murilo Dantas 300 SE Aracaju Brazil Food Research Laboratory Institute of Technology and Research Tiradentes University Av. Murilo Dantas 300 SE Aracaju Brazil Federal Institute of Education Science Technology of Sergipe Av. Lourival Batista SE Lagarto Brazil Lucknow226 025 India Research Institute of Integrative Life Sciences Dongguk University–Seoul Ilsandong-gu Goyang–si Gyeonggido10326 Korea Republic of Department of Food Science and Biotechnology Dongguk University-Seoul Ilsandong-gu Goyang-si Gyeonggido10326 Korea Republic of Graduate Program in Genomic Sciences and Biotechnology Catholic University of Brasília Brasília71.966-700 Brazil

Amid the global water stress affecting billions, the importance of saltwater desalination technologies is evident, given the abundance of seawater on Earth. Solar distillation emerges as a sustainable solution, harnessing renewable energy and employing user-friendly equipment. This study focuses on enhancing self-sustaining technologies reliant on solar energy, such as solar collectors, aluminum oxide/water nanofluid, a basin coated with titanium dioxide nanoparticles, and copper fins for improved heat management. These modifications yielded a remarkable 58% increase in the daily production of distilled water. In addition, an Artificial Neural Network (ANN) model was developed, utilizing inputs such as solar radiation, wind speed, ambient and basin temperature, and glass cover temperature to forecast water production. The robustness of the ANN was validated through a high coefficient of determination (0.9948) and low errors (mean absolute error: 2.2174, mean squared error: 3.0210), underscoring its practical utility in predicting desalination system productivity prior to implementation. Furthermore, Garson's algorithm was employed to assess the significance of process variables, providing valuable insights into system optimization. Overall, this research offers a comprehensive exploration of advancements in solar distillation technologies, emphasizing their potential for addressing water scarcity challenges and paving the way for sustainable water management practices. © 2024, The Authors. All rights reserved.

关键词： Aluminum oxide

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Electrospun Nanofibers from Poly (Ε-Caprolactone) with Copaiba (Copaifera Sp)Oleoresin as an Advanced Healer

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SSRN 2024年

作者： Marcos Pinho da Silva, Andrey Miotto, Marília Afonso Provenzi, Marcel Lopes, Susane Maraschin, Marcelo Merlini, Claudia Sciences and Materials Engineering Graduate Program Federal University of Santa Catarina Reitor João David Ferreira Lima University Campus SC Florianópolis88040-900 Brazil Department of Materials Engineering Technology College Federal University of Amazonas AM Manaus Brazil Food Microbiology Laboratory Departament of Food Science and Technology Federal University of Santa Catarina Agrarian Sciences Center Av. Admar Gonzaga 1346 - Itacorubi SC . Florianópolis88034-001 Brazil Florianópolis Brazil Rua João Pessoa 2750 Bairro Velha Blumenau SC 89036-256 Brazil

Advanced healing developed using electrospinning show advantages such as highsurface area/volume relation, similarity with the skin extracellular matrix and ability toincorporate bioactive agents. The use of natural compounds, as the copaiba oleoresinresults in benefits due to its action as enhancer of biochemical agents during theinflammatory phase in the healing process. This study investigated the development ofnanofiber from polycaprolactone and copaiba oleoresin aiming at demonstrating itsantimicrobial and atoxic properties with healing effect on complex wounds. Thecopaiba oleoresin chemical composition revealed the predominance of sesquiterpenes,with majoritary components such as Caryophyllane, γ-Muurolene, and α-Copaene. Thenanofibers were anlyzed employing several techniques and revealed suitablephysicochemical characteristics for the development of advanced healing. The zetapotential indicated a negative surface load in the nanofibers, suggesting favorableinteractions for biomedical applications such as cell adherence. The copaiba oleoresinpresence significantly increased the liquid absorption ability, fundamental in advancedhealing, and accelerated the mass loss rate, emphasizing its influence in thebiodegradability of the nanofibers. The nanofibers showed efficacy againstStaphylococcus Aureus, while the cell viability assay indicared absence of *** results suggest the potential of these nanofibers as efficient and sustainableantimicrobial healing for clinical applications © 2024, The Authors. All rights reserved.

关键词： Nanofibers

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Investigation of Cyanobacteria-Hosted Antibiotic Resistance Genes in Cyanohab-Impacted Drinking Water Sources

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SSRN 2025年

作者： Volk, Abigail Mills, Molly Chae, Soryong Lee, Jiyoung Division of Environmental Health Sciences College of Public Health The Ohio State University ColumbusOH United States Environmental Sciences Graduate Program The Ohio State University ColumbusOH United States Department of Chemical and Environmental Engineering University of Cincinnati CincinnatiOH United States Department of Food Science and Technology The Ohio State University ColumbusOH United States Infectious Diseases Institute The Ohio State University ColumbusOH United States

Freshwater cyanobacterial blooms (cyanoHABs) produce cyanotoxins that cause human and animal illness. cyanoHABs are also hypothesized to play a role in the antibiotic resistome, but whether Cyanobacteria host clinically-relevant antibiotic resistant genes (ARGs) in the environment is largely unknown. To investigate this emerging issue, we looked for the presence of Cyanobacteria-hosted ARGs using shotgun metagenomic sequencing of drinking water source samples collected during the 2022 bloom season from Lake Erie and Grand Lake St. Marys (GLSM). . ARGs were annotated using DeepARG and Resistance Gene Identifier (RGI). Cyanobacteria were annotated to host genes conferring possible antibiotic resistance. Two of these genes were rpoB2 and arlR, but these genes were likely spurious hits on housekeeping genes. qac and OXA genes were also annotated. While those hits suggest potential resistance to clinical antibiotics, overall Cyanobacteria did not host appreciable clinically-relevant ARGs. Because the two drinking water sources historically have high cyanotoxin concentrations, selected cyanotoxins, Cyanobacteria, and ARGs were chosen for quantification. We found high levels of Microcystis in Lake Erie and Planktothrix in GLSM, and high microcystin concentrations in GLSM, supporting previous trends in these water bodies. This study takes a novel approach pairing the issues of cyanoHABs and ARGs together in two drinking water sources. In a changing climate, drinking water treatment strategies should consider the treatment and public health implications of multiple contaminants. © 2025, The Authors. All rights reserved.

关键词： Lakes

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Is it Possible to Produce Canned Champignon Mushrooms with Less Sulfite?

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SSRN 2023年

作者： Coelho, Vinicius Serafim Modolo, Mariana Uliana de Jesus Filho, Milton Macedo, Leandro Levate Cardoso, Wilton Soares Pinheiro, Flávia de Abreu Federal Institute of Espírito Santo Venda Nova do Imigrante Espírito Santo Brazil Graduate Program in Food Science and Technology Center for Agricultural Sciences and Engineering Federal University of Espírito Santo Espírito Santo Alegre Brazil Department of Pharmacy Federal University of Juiz de Fora Governador Valadares Minas Gerais Governador Valadares Brazil

This study aimed to reduce sulfite levels in canned mushroom (Agaricus bisporus). Formulations were developed: without sulfite and condiment (F1);without sulfite and with condiment (F2);with sulfite and without condiment (F3);with sulfite and condiment (F4). Control (C) consisted of traditional canned mushrooms prepared by the producer. The treatments were evaluated over 42 days. Acidity and pH analysis showed that canned C and F1 maintained equilibrium pH ( © 2023, The Authors. All rights reserved.

关键词： Fungi

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Mogroside V inhibits LPS-induced COX-2 expression/ROS production and overexpression of HO-1 by blocking phosphorylation of AKT1 in RAW264.7 cells

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生物化学与生物物理学报（英文版） 2019年第4期51卷 365-374页

作者： Yong Li Luyan Zou Tao Li Dengni Lai Yanyang Wu Si Qin Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China The United Graduate School of Agricultural Sciences Faculty of Agriculture Kagoshima University Korimoto 1-21-24 Kagoshima 890-0065 Japan

Momordica grosvenori is a valuable edible plant with medicinal purposes,and it is widely used in medicated diets and traditional Chinese medicine in *** V (MV),the main bioactive component from ***,is commonly used as a natural *** extracts have been reported to exert potent anti-inflammatory property,however the underlying molecular mechanism still remains *** the present study,the biological effect of MV in inflammation was investigated in lipopolysaccharide (LPS)-stimulated RAW264.7 *** ELISA and western blot analysis results showed that MV significantly inhibited LPS-induced prostaglandin E2 (PGE2) production and cyclooxygenase-2 (COX-2) *** markedly decreased the phosphorylation of IκB-α,increased IκB-α,and reduced nuclear p-65 and C/EBPδ.Furthermore,MV attenuated LPS-induced phosphorylation of MAPKs and AKT1,and only the phosphorylation status of AKT1 was found to be consistent with the expression trend of ***,MV reduced ROS level and restored overexpressed HO-1 and AP-1 to basal level,which can be markedly reversed by AKT1 inhibitor *** results revealed that AKT1 plays a key role in LPS-induced COX-2 expression,and acts as a mediator to keep the redox balance in LPS-stimulated RAW264.7 *** exerts anti-inflammatory property by blocking AKT1-mediated NF-κB and C/EBPδ activation,ROS generation and AP-1/HO-1 ***,the present study indicated that MV has a significant chemopreventive effect on the inflammatory lesions and suggested that AKT1 is a potential specific target of MV for relieving inflammation.

关键词： Mogroside V anti-inflammatory property HO-1 NF-κB AKT1 ROS

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Nutritional and Technological Properties of Albino Peach Palm (Bactris Gasipaes) from the Amazonian: Influence of Cooking and Drying

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SSRN 2023年

作者： Soares, Stephanie Dias dos Santos, Orquídea Vasconcelos da Conceição, Leyvison Rafael Vieira Costi, Hilton Túlio Júnior, José Otávio Carrera Silva do Nascimento, Francisco das Chagas Alves Pena, Rosinelson da Silva Graduate Program in Food Science and Technology Federal University of Pará Belém Brazil Faculty of Nutrition Institute of Health Sciences Federal University of Pará Belém Brazil Faculty of Chemistry Institute of Exact and Natural Sciences Federal University of Pará Belém Brazil Emílio Goeldi Museum Coordination of Earth Sciences and Ecology Belém Brazil Faculty of Pharmacy Institute of Health Sciences Federal University of Pará Belém Brazil Faculty of Food Engineering Institute of Technology Federal University of Pará Belém Brazil

This study aimed to submit the albino peach palm to the cooking and drying processes, and characterize the raw pulp (RP), cooked pulp (CP), raw pulp flour (RPF), and cooked pulp flour (CPF). The product's chemical composition, bioactive compounds, and physical-chemical, color, thermal, morphological, and functional-technological properties were evaluated. The proximate composition showed that carbohydrates were the main constituents of all products. The cooking process increased the moisture content of the pulp but decreased its other contents. The colorimetric parameters indicated a significant browning. After cooking, the total phenolic compounds and ascorbic acid content decreased in the pulp. The RPF and CPF presented different thermogravimetric behaviors. The spectral patterns in the infrared region showed characteristic bands of organic compounds which are present in the structure of starches. Scanning electron microscopy showed amyloplast and fiber bundles with starches in the RP and gelatinized starch granules in the CP and CPF. The RPF presented small and heterogeneous starch granules with isolated amyloplast. The RPF and CPF showed different granulometric patterns and technological indices. The results indicated that pulp and flour from the pulp of albino peach palms can be exploited by the food, pharmaceutical, and biotechnological industries. © 2023, The Authors. All rights reserved.

关键词： Fruits

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Investigation of Cyanobacteria-Hosted Antibiotic Resistance in Drinking Water Sources from a Public Health Perspective

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SSRN 2024年

作者： Volk, Abigail Mills, Molly Chae, Soryong Lee, Jiyoung Division of Environmental Health Sciences College of Public Health The Ohio State University ColumbusOH United States Environmental Sciences Graduate Program The Ohio State University ColumbusOH United States Department of Chemical and Environmental Engineering University of Cincinnati CincinnatiOH United States Department of Food Science & Technology The Ohio State University ColumbusOH United States Infectious Diseases Institute The Ohio State University ColumbusOH United States

Cyanobacterial blooms are intensifying worldwide due to eutrophication and climate change. While traditionally, the focus has been on cyanotoxins, recent studies suggest a potential link between cyanobacteria and antibiotic resistance (AR), including hosting AR genes (ARGs) or promoting gene transfer. However, the role of cyanobacteria as ARG hosts and their contribution to environmental AR in drinking water sources remains largely unexplored. In this study, we examined cyanobacteria as ARG hosts within the broader microbiome context. Samples of drinking water sources were collected from Lake Erie and Grand Lake St. Marys (GLSM) between May and October 2022, both sites afflicted historically by blooms. Cyanobacterial genes and ARGs were quantified with DropletDigital™ PCR (ddPCR), while microcystin concentrations were quantified with enzyme-linked immunosorbent assay (ELISA). Shotgun metagenomic sequencing was employed to analyze the microbiome, with ARGs annotated using DeepARG and Resistance Gene Identifier. Although the alpha diversity and composition of the microbial communities were similar at both locations, the ddPCR and sequencing revealed elevated levels of Microcystis in Lake Erie and Planktothrix in GLSM. The ELISA results showed high microcystin concentrations in GLSM. Cyanobacteria were identified as potential hosts for ARGs, including genes such as rpoB2 and arlR. However, upon further examination, these genes are likely annotated on housekeeping genes and are considered false positives spurious hits. In addition, qac and OXA genes were also annotated, consistent with previous findings on NCBI BLAST in Cyanobacteria. Although cyanobacteria do not appear to be significant contributors to the resistome in these contexts, they may confer some antibiotic resistance. This study underscores the importance of continued monitoring of cyanobacteria within the broader microbiome and resistome, especially in drinking water sources with high contamination levels.

关键词： Lakes

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Violacein quantification using a smartphone coupled to an endoscopic camera: Quorum sensing inhibition in Chromobacterium violaceum

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Microbe (Netherlands) 2025年 6卷

作者： Casagrande, Camilla Voos, Monica Nunes, Franciane Batista dos Santos Alves, Camilla Filippi Verdi, Camila Marina Barin, Juliano Santos, Roberto Christ Vianna Department of Microbiology and Parasitology Federal University of Santa Maria (UFSM) Santa Maria Brazil Department of Food Science and Technology Federal University of Santa Maria Rio Grande do Sul Santa Maria Brazil Laboratory of Oral Microbiology Research (LAPEMICRO) Federal University of Santa Maria Rio Grande do Sul Brazil Graduate Program in Pharmaceutical Sciences Federal University of Santa Maria Rio Grande do Sul Brazil

Quorum sensing (QS) strongly regulates the biofilm formation mechanism in several microorganisms. The blockade of violacein production in Chromobacterium violaceum has been employed to indicate QS inhibition. Nevertheless, quantifying violacein is challenging due to the reliance on high-cost equipment and supplies. Consequently, this study compared the production and inhibition of violacein using a traditional spectrophotometric method and an alternative approach utilizing a Photometrix UVC Android application integrated with a USB endoscopic camera. The study standardized the growth of C. violaceum in several culture media and incubation times, then evaluated the pigment inhibition against salicylic acid. Results indicated that C. violaceum produced more violacein when cultivated in BHI broth for 24 h. Salicylic acid inhibited violacein production, even at sub-inhibitory concentrations, with a minimum inhibitory pigment concentration (125 µg/mL) and a minimum bactericidal concentration (2000 µg/mL). A comparison between application-based readings and the traditional method showed no significant differences, highlighting the potential of this technology as an essential tool in for QS inhibition and biofilm research. © 2025

关键词： Biofilm Colorimetric methods Smartphone Violacein

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