检索结果-内蒙古大学图书馆

Hepatitis B virus HBcAg gene transformtion in Escherichia coli DH5α using pEGFP-N1

AIP Conference Proceedings 2024年第1期3068卷

作者： Dini Cahyani Lalu Unsunnidhal Tri Untari Asmarani Kusumawati Master Program of Biotechnology Graduate School of Universitas Gadjah Mada Jalan Teknika Utara Kocoran Caturtunggal Depok Sleman Yogyakarta 55281 Indonesia Food Technology Study Program Faculty of Food Technology and Agroindustry University of Mataram Mataram 83125 Indonesia Biomedical Field Nursing Study Program STIKES Yarsi Mataram West Nusa Tenggara 83361 Indonesia Departement of Microbiology Faculty of Veterinary Medicine Universitas Gadjah Mada Yogyakarta 55281 Indonesia Department of Reproduction and Obstetrics Faculty of Veterinary Medicine Universitas Gadjah Mada Yogyakarta 55281 Indonesia Biotechnology Study Program Graduate School of Universitas Gadjah Mada Yogyakarta 55281 Indonesia

Hepatitis B virus is a virus that attacks humans and it can cause liver cancer. Hepatitis B subgenotype B3 is commonly found in Southeast Asia. Thus, the development of a vaccine is carried out to prevent the spread of the Hepatitis B virus in Indonesia. The purpose of this study was to transform the hepatitis B virus HBcAg gene using the pEGFP-N1 vector on Escherichia coli DH5α as host cells. The method in this research was codon optimization and plasmid transformation of recombinant DNA in pEGFP-N1. The recombinant cloning was divided into two stages, that was making competent cells and transforming recombinant DNA plasmids. The HBcAg gene was obtained from the results of isolation and cloning that had been carried out by previous researchers, would be constructed on the pEGFP-N1 vector. The results obtained in this study were that the HBcAg gene was successfully cloned and transformed into Escherichia coli DH5α (pEGFP-N1-HBcAg) by heat shock method using MgCl2−CaCl2 reagent. Based on the results of PCR colonies which stated that the transformant bacteria E. coli DH5α succeeded in carrying the pEGFP-N1-HBcAg plasmid marked by the presence of DNA bands with a length of 152 bp. Therefore, the construction of pEGFP-N1-HBcAg using Escherichia coli host cells can be used as a DNA vaccine candidate.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Enhancing Diagnostic Accuracy for Knee Osteoarthritis Using a Deep Learning Approach

Enhancing Diagnostic Accuracy for Knee Osteoarthritis Using ...

引用

International Symposium on Applied Science 2024, ISAS 2024

作者： Raza, Aquib Ching, Congo Tak Shing Liu, Peng-Ta Phan, Thien-Luan Tran, Ngoc-Luan Nguyen, Ha Anh T. Rameez, Mohammad Li, Hung-Chung Linh, Dao Nguyen Phuong Van Hieu, Nguyen Tran, Trung Nghia National Taiwan University Taipei City10673 Taiwan Research Center for Information Technology Academia Sinica Taipei City11529 Taiwan Graduate Institute of Biomedical Engineering National Chung Hsing University Taichung402 Taiwan Department of Electrical Engineering National Chi Nan University Nantou County Taiwan Advanced Plant and Food Crop Biotechnology Center National Chung Hsing University Taichung402 Taiwan International Doctoral Program in Agriculture National Chung Hsing University Taichung402 Taiwan Fall Prevention Center Department of Physical Medicine & Rehabilitation Changhua Christian Hospital Changhua500 Taiwan Department of Physics and Electronic Engineering University of Science Vietnam National University - Ho Chi Minh City Ho Chi Minh City70000 Viet Nam Department of Computer Science University of York York HeslingtonYO10 5DD United Kingdom Undergraduate Program of Intellectual Creativity Engineering National Chung Hsing University Taichung402 Taiwan Ho Chi Minh City72409 Viet Nam HCMUT Vietnam National University Ho Chi Minh City Thu Duc Ho Chi Minh City71308 Viet Nam

This research introduces a deep learning framework that combines convolutional neural networks with autoencoders to improve the diagnostic accuracy of knee osteoarthritis. The study utilized a publicly available dataset from the Mendeley Data repository, where knee joint images were preprocessed using Contrast Limited Adaptive Histogram Equalization to enhance contrast and ensure consistent image sizing. Initially, an autoencoder was employed in an unsupervised manner to learn compressed representations of the data. These representations were then used to train a CNN to classify knee images into 'healthy' and 'osteoarthritic' categories. The model was trained using an 80:20 split for training and testing data, incorporating early stopping and data augmentation techniques to prevent overfitting and enhance generalization. Performance metrics such as accuracy, precision, recall, and the area under the Receiver Operating Characteristic curve (ROCAUC) were used to evaluate the model, resulting in a high test accuracy of 98.95%. The high precision and recall rates further confirmed the model's robustness in diagnosing KOA. To enhance interpretability, Gradient-weighted Class Activation Mapping (Grad-CAM) was applied to identify critical regions in the images that influenced the model's decisions. Additionally, t-Distributed Stochastic Neighbor Embedding (t-SNE) was used to visualize the high-dimensional encoded features in a two-dimensional space, providing deeper insights into how the model differentiates between classes. The promising results of this deep learning approach suggest its potential for broader applications in medical imaging diagnostics. By providing healthcare professionals with a tool that enhances diagnostic accuracy and operational efficiency, this framework lays the foundation for developing scalable, AI-driven diagnostic systems for various complex medical conditions. © 2025 Institute of Physics Publishing. All rights reserved.

关键词： Joints (anatomy)

来源：评论

学校读者我要写书评

暂无评论

Transformation of HBcAg gene of hepatitis B virus using pEGFP-C1 vector

引用

AIP Conference Proceedings 2023年第1期2956卷

作者： Umi Barokah Lalu Unsunnidhal Yuli Purwandari Kristianingrum Asmarani Kusumawati Master Program of Biotechnology Graduate School of Universitas Gadjah Mada Sleman Yogyakarta. Indonesia Food Technology Study Program Faculty of Food Technology and Agroindustry University of Mataram Mataram. Indonesia Biomedical Field Nursing Study Program STIKES Yarsi Mataram West Nusa Tenggara Indonesia Department of Pathology Faculty of Veterinary Medicine University of Gajah Mada Yogyakarta Indonesia Department of Reproduction and Obstetrics Faculty of Veterinary Medicine Universitas Gadjah Mada Yogyakarta Indonesia

The HBcAg gene is a specific gene for the detection of hepatitis B virus (HBV). Antibodies against HBcAg are the most reactive among other antibodies. DNA transformation is the process of inserting DNA into a bacterial cell through the transfer of a plasmid which contains the genetic material. This study aims to evaluate the results of the transformation of the pEGFP-C1 plasmid containing the HBcAg gene. This research begins with molecular cloning of E. coli DH5α bacteria. E. coli DH5α was prepared to become competent cells. In addition, the plasmid containing the HBcAg gene was transformed into E. coli DH5α host cells by the heat shock method. Transformation was assessed by the presence of E. coli DH5α on LB agar containing the antibiotic kanamycin. Gene conjugated plasmids in E. coli DH5a were analyzed by 1% agarose gel electrophoresis. The results of this study indicated that the transfection of the pEGFP-C1 plasmid containing the HBcAg gene into E. coli DH5α was successfully carried out, marked by the growth of E. coli DH5α colonies. coli DH5α on LB agar containing the antibiotic kanamycin. The picture on the agarose gel shows that the plasmid carrying the HBcAg gene can be transformed into E. coli DH5α bacterial cells.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway

引用

生物化学与生物物理学报（英文版） 2019年第1期51卷 31-40页

作者： Tao Li Qili Li Weiguo Wu Yong Li De-xing Hou Hua Xu Baodong Zheng Shaoxiao Zeng Yang Shan Xiangyang Lu Fangming Deng Si Qin Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China Hunan Agricultural Product Processing Institute Hunan Academy of Agricultural Sciences Changsha 410125 China Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China The United Graduate School of Agricultural Sciences Faculty of Agriculture Kagoshima University Kagoshima 890-0065Japan Department of Pediatrics Steele Children's Research Center University of Arizona Tucson 85724 USA Department of Food Safety College of Food Science Fujian Agriculture and Forestry University Fuzhou 350002 China Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China The United Graduate School of Agricultural Sciences Faculty of Agriculture Kagoshima University Kagoshima 890-0065Japan Department of Pediatrics Steele Children's Research Center University of Arizona Tucson 85724 USA

Lotus seed is well known as traditional food and medicine,but its skin is usually *** studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions,such as antioxidation,anti-inflammation,and anti-cancer *** the present study,we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation,identify their chemical structures by HPLC-MS-MS and NMR,and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in *** results showed that PMR mainly contained oligomeric proanthocyanidins,especially dimeric procyanidin B1 (PB1),procyanidin B2 and procyanidin *** it had limited ability to directly scavenge radicals in vitro,PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 *** data revealed that PB1,a major component in PMR,stabilized Nrf2 by inhibiting the ubiquitination of Nrf2,which led to subsequent activation of the Nrf2-ARE pathway,including the enhancements of Nrf2 nuclear translocation,Nrf2-ARE binding and ARE transcriptional ***,the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of *** results revealed that lotus seed skin is a promising resource for functional food development.

关键词： lotus seed skin extract proanthocyanidin Nrf2-ARE pathway antioxidant activity

来源：评论

学校读者我要写书评

暂无评论

Corrigendum to “Trends in quality assessment and drying methods used for fruits and vegetables” [food Control 142 (2022) 109254]

引用

food Control 2024年 166卷

作者： Felipe Richter Reis Caroline Marques Ana Carolina Sales de Moraes Maria Lucia Masson Food Technology Course Federal Institute of Paraná R. Antônio Chemin n. 28 83403-515 Colombo PR Brazil Department of Chemical Engineering Graduate Program in Food Engineering (PPGEAL) Federal University of Paraná Av. Francisco Heráclito dos Santos n. 100 81531-980 Curitiba PR Brazil

来源：评论

学校读者我要写书评

暂无评论

Nanoparticle-based therapeutic strategies for chronic liver diseases: Advances and insights

引用

Liver Research 2025年第2期9卷 104-117页

作者： Sathiyamoorthy Padmanaban Ji-Won Baek Sai Sahithya Chamarthy Saipriya Chandrasekaran Antony V Samrot Vijayakumar Gosu In-Kyu Park Kamalakannan Radhakrishnan Don-Kyu Kim Department of Biomedical Sciences and BioMedical Sciences Graduate Program (BMSGP) Chonnam National University Medical School Gwangju Republic of Korea Host-directed Antiviral Research Center Department of Integrative Food Bioscience and Biotechnology (BK21 FOUR) Chonnam National University Gwangju Republic of Korea Department of Obstetrics Gynecology and Reproductive Sciences Sanford Consortium for Regenerative Medicine University of California San Diego (UCSD) San Diego CA USA Department of Neuroscience University of California San Diego (UCSD) San Diego CA USA Department of Microbiology Faculty of Medicine Manipal University College Malaysia Melaka Malaysia Department of Animal Biotechnology Jeonbuk National University Jeonju Republic of Korea Host-directed Antiviral Research Center School of Biological Sciences and Technology Chonnam National University Gwangju Republic of Korea

The liver is pivotal in protein synthesis, glucose and lipid metabolism, and detoxification. However, the liver is susceptible to both acute and chronic disorders, with chronic conditions being fatal. Chronic liver diseases (CLDs), such as liver fibrosis, which usually represents the early manifestation of cirrhosis, primarily result from hepatitis B and C viruses infections, metabolic disorders, alcohol abuse, immune-mediated attacks, and cholestatic injury. The progression of liver fibrosis contributes to the development of cirrhosis, which can further lead to hepatocellular carcinoma, portal hypertension, hepatic decompensation, and hepatic encephalopathy. The extracellular matrix deposition over time leading the hepatocyte necrosis (cirrhosis) is the main structural feature of CLDs and may cause hepatic failure. Certain conditions, such as hepatitis and autoimmune diseases, may promote the rapid deterioration of liver function. Acute and chronic liver failure causes may vary, with early referral for liver transplantation improving the chance of recovery. The healthcare system need improvements to manage patients with non-alcoholic fatty liver disease and alcoholic fatty liver disease, as they have the potential to progress to cirrhosis. Both conditions involve the release of reactive oxygen species and damage-associated molecular patterns from cytokines, hepatic stellate cells, and hepatocyte autophagy, leading to prolonged inflammation. While various medications target fibrosis and liver damage, nanoparticle-based drug delivery systems offer additional promise by promoting faster liver regeneration. This review provides a comprehensive overview of the potential of nanoparticle systems as a future therapeutic approach for treating liver disorders.

关键词： Chronic liver diseases (CLDs) Reactive oxygen species (ROS) Antioxidants Nanoparticles

来源：评论

学校读者我要写书评

暂无评论

Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell

Food Chemistry Advances

引用

food Chemistry Advances 2024年 5卷

作者： Adefegha, Stephen Adeniyi Mostardeiro, Vitor Morsch, Vera Maria Morel, Ademir F. Da Cruz, Ivana Beatrice Manica Somacal, Sabrina Oboh, Ganiyu Schetinger, Maria Rosa Chitolina Functional Foods Nutraceuticals and Phytomedicine Laboratory Department of Biochemistry Federal University of Technology P. M. B. 704 Akure Nigeria Graduate Program in Toxicological Biochemistry Department of Biochemistry and Molecular Biology Center of Natural and Exacts Sciences Federal University of Santa Maria RS Santa Maria 97105-900 Brazil Department of Chemistry Center of Natural and Exacts Sciences Federal University of Santa Maria RS 97105-900 Brazil Biogenomics Laboratory Department of Morphology Federal University of Santa Maria RS 97105-900 Brazil Department of Food Science and Technology Center for Rural Sciences Federal University of Santa Maria RS 97105-900 Brazil

Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1–100 µg/ml) for cell viability assay and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents. Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. In conclusion, this study suggests that M. stenopetala extract is rich in antioxidant and contains bioactive neuroprotective agents. © 2024 The Author(s)

关键词： Antioxidant BV-2 microglial cell Cell viability Moringa stenopetala Oxidative stress

来源：评论

学校读者我要写书评

暂无评论

Cellucalst enzyme-assisted extraction of Sargassum horneri enhances the immunomodulation by regulating TLR4/MyD88/NF-kB pathway in murine splenocytes with or without Concanavalin A

引用

Biomedicine and Pharmacotherapy 2024年 181卷 117673-117673页

作者： Yang, Jiwon Kim, Hyo Jin Herath, Kalahe Hewage Iresha Nadeeka Madushani Jee, Youngheun Interdisciplinary Graduate Program in Advanced Convergence Technology & Science Jeju National University Jeju 63243 South Korea Department of Food Bioengineering Jeju National University Jeju 690-756 South Korea Department of Environmental Health Harvard T.H. Chan School of Public Health Harvard University Boston Massachusetts United States Department of Biosystems Engineering Faculty of Agriculture and Plantation Management Wayamba University Gonawila Makandura 60170 Sri Lanka Department of Veterinary Medicine and Veterinary Medical Research Institute Jeju National University Jeju 64243 South Korea

Sargassum horneri (S. horneri) is an edible species of large brown algae inhabiting along the coasts of northeastern Asia. The study focuses on the impact of celluclast enzyme extract of S. hoeneri (SHC) on various immune cell populations in splenocytes including granulocytes, macrophages, dendritic cells, and T lymphocytes. SHC alone increased the population of granulocytes and macrophages and the secretion of M1 macrophage-derived cytokines (TNF-α, IL-22), and M2 macrophage-derived cytokines (IL-4, IL-10). Interestingly, however, SHC suppressed the concanavalin A (Con A)-expanded populations of macrophages, dendritic cells, granulocytes, T and B cells, and Con A-promoted secretion of M1-macrophage derived cytokines (IFN-γ, IL-1β, TNF-α, IL-17, IL-22) and M2-macrophage derived cytokines (IL-4, IL-10, IL-13, TGF-β). SHC further restrained the Th1, Th2, and Th17 cell responses through attenuating the expression of respective transcription factors T-bet, Gata3, and Rorγt. The anti-inflammatory property of SHC is highlighted through its influence on cytokine production, particularly in the NF-κB pathway, and the attenuation of Toll-like receptor (TLR) signaling. The results reveal that SHC acts as both an immunostimulator and an inhibitor of hyperimmune reactions, showcasing its potential therapeutic applications in conditions involving dysregulated immune responses such as autoimmune diseases and inflammatory disorders. This positions SHC as a promising candidate for the development of functional ingredients with diverse applications encompassing the realms of food, pharmaceuticals, and cosmetics. © 2024

关键词： Celluclast Concanavalin A S. horneri Splenocytes

来源：评论

学校读者我要写书评

暂无评论

Dual-Targeting EGCG/NO-Supplying protein assembled nanoparticles with Multi-Synergistic effects against atherosclerosis

引用

Chemical Engineering Journal 2024年 493卷

作者： Lin, Chi Cheng, Tsai-Mu Liu, Yun-Chun Hsu, Fang-Yu Shih, Chun-Ming Tsai, Min-Lang Shih, Chun-Che Mi, Fwu-Long Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei11031 Taiwan The PhD Program for Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei11031 Taiwan Taipei Heart Institute Taipei Medical University Taipei11031 Taiwan Department of Food Science National Taiwan Ocean University Keelung20224 Taiwan Department of Biochemistry and Molecular Cell Biology School of Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital Taipei11031 Taiwan Division of Cardiovascular Surgery Department of Surgery Wan Fang Hospital Taipei Medical University Taipei11031 Taiwan Institute of Clinical Medicine School of Medicine National Yang-Ming Chiao Tung University Taipei Taiwan Department of Surgery School of Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan Graduate Institute of Nanomedicine and Medical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan International Ph.D. Program for Cell Therapy and Regeneration Medicine College of Medicine Taipei Medical University Taipei Taiwan

Limited therapeutic options are available to effectively preventing atherosclerosis. Inflammatory endothelial cells, foamy macrophages, and high protease levels contribute to atherosclerotic plaque formation. Studies have shown that catechins effectively scavenge reactive oxygen species (ROS), inhibit monocyte adhesion and reduce cholesterol levels, while nitric oxide (NO) enhances endothelial function. However, due to the poor stability and bioavailability of catechins and the toxicity from the burst release of current synthetic small molecules NO donor, effective delivery of these bioactive compounds to treat atherosclerosis is still a challenge. Herein, a catechin/protein-based NO donor co-delivery nanosystem was designed for combinatorial anti-atherosclerotic therapy. We engineered a (−)-epigallocatechin-3-gallate (EGCG)/NO-releasing protein co-assembled nanocomplex based on specific catechin-protein interactions. Furthermore, the nanocomplex was surface modified with fucoidan (Fu), a sulfated polysaccharide with anti-inflammatory activity. This nanocomplex exhibits sensitivity to ROS, pH, and enzymes. The Fu-functionalized nanoparticles specifically accumulates in atherosclerotic plaques mediated by P-selectin on inflamed endothelial cells and scavenger receptor A (SR-A) on foamy macrophages. Under environmental stimuli that simulate the condition of plaque, the nanoparticles are readily activated to release EGCG and NO in response to excess ROS and high protease levels, exerting the multi-synergistic anti-atherosclerosic effects on reducing monocyte adhesion, promoting NO production to proliferate endothelial cells, lowering ROS levels, and decreasing the foam cell formation in vitro, and reducing lipid accumulation, plaque size, and inflammatory cytokines release in high-fat diet-induced atherosclerosis model in ApoE−/− mice. The integration of plaques targeting ability and multiple therapeutic functions can provide an advanced therapeutic strategy for athero

关键词： Nitric oxide

来源：评论

学校读者我要写书评

暂无评论

Transformation and cloning of dengue virus’s rE gene using pEGFP-C1 as vector in Escherichia coli DH5α

引用

AIP Conference Proceedings 2024年第1期3080卷

作者： Stephanie Fadilatun Humairah Piliang Lalu Unsunnidhal Sitti Rahmah Umniyati Asmarani Kusumawati Master Program of Biotechnology Graduate School Universitas Gadjah Mada Yogyakarta 55281 Indonesia Food Technology Study Program Faculty of Food Technology and Agroindustry University of Mataram Mataram 83125 Indonesia Biomedical Field Nursing Study Program STIKES Yarsi Mataram West Nusa Tenggara 83361 Indonesia Department of Parasitology Faculty of Medicine Public Health and Nursing Universitas Gadjah Mada Yogyakarta 55281 Indonesia Centre for Tropical Medicine Faculty of Medicine Public Health and Nursing Universitas Gadjah Mada Yogyakarta 55281 Indonesia Department of Reproduction and Obstetrics Faculty of Veterinary Medicine Universitas Gadjah Mada Yogyakarta 55281 Indonesia

Dengue Virus (DENV) infection now has become a major public health threat, with nearly majority of population around the globe at high risk of infection. Effective treatments and drugs against DENV infection have not been established yet. With the emergence of the first licensed vaccine, Dengvaxia® (CYD-TDV), became the start to the development of several vaccine candidates. Hence, with the advent of recombinant DNA technology, DNA vaccines are such a promising approach compared to conventional vaccine methods. The recombinant multivalent protein envelope of DENV, known as rE, was constructed based on protein envelope domain III of four serotypes DENV and was reported structurally stable as well as potentially immunogenic. This research is conducted as an early stage in the development of DNA vaccine candidates against DENV, by transforming optimized rE gene mediated with plasmid vector pEGFP-C1 using a brief heat-shock treatment in E. coli DH5α as cloning host. Competent cells were produced using the combination of Magnesium Chloride and Calcium Chloride solution. The results showed growth signs of transformant colonies on a selective media containing kanamycin 100µg/ml, relatively. Colonies was confirmed carrying the target gene by colony polymerase chain reaction (PCR) screening. Observation on agarose gel under UV transIlluminator indicate bands that appeared in the product size of 157 base pairs.

关键词：

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：