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Quality and production enhancement of fish mint, Houttuynia cordata Thunb., cultivated in a hydroponic planting system with designed plant growth-promoting additives

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Heliyon 2024年第7期10卷 e28755页

作者： Loo, Yen Chi Tsai, Yi-Hong Chen, Hsieh Hsieh, Hui-Ping Chen, Yen-Chang Chen, Hsueh-Er Lin, Zhi-Hu Huang, Hung-Tse Liu, I.-M. Liaw, Chia-Ching Chang, Fang-Rong Graduate Institute of Natural Products College of Pharmacy Kaohsiung Medical University Kaohsiung 807378 Taiwan Department of Pharmacy and Master Program College of Pharmacy and Health Care Tajen University Pingtung County 907101 Taiwan Graduate Institute of Science Education and Environment Education College of Science National Kaohsiung Normal University Kaohsiung 807 Taiwan Department of Research and Development We-Win Bio-Medical Co. Ltd. Kaohsiung 807 Taiwan Division of Materia Medica Development National Research Institute of Chinese Medicine Taipei 112026 Taiwan Institute of Traditional Medicine National Yang Ming Chiao Tung University Taipei 112026 Taiwan Department of Biochemical Science and Technology National Chiayi University Chiayi 600355 Taiwan Department of Medical Research Kaohsiung Medical University Hospital Kaohsiung 807377 Taiwan Drug Development and Value Creation Research Center Kaohsiung Medical University Kaohsiung 807378 Taiwan Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung 804201 Taiwan

Fish mint, Houttuynia cordata Thunb. (HCT) is an edible vegetable that has also been used in traditional folk medicines. As both a medicinal herb and a dietary source, HCT has been clinically proven to be a pivotal ingredient in formulas administered to alleviate COVID-19 symptoms. With the increasing market demand for imported materials, ensuring the quality consistency of HCT becomes a significant concern. In this study, the growing time for hydroponically-cultivated HCT with seaweed extract and amino acids added (HCTW) reduced by half compared to conventional soil-cultivated HCT (HCTS). Key quantified components in HCTW, flavonoid glycosides and caffeoylquinic acid derivatives, exhibited a 143% increase over HCTS. These crucial constituents were responsible for possessing antioxidant activity (IC₅₀ < 25 μg/mL) and anti-nitrite oxide production (IC₅₀ < 20 μg/mL). An economically-designed hydroponic system with appropriate additives is proposed to replace HCTS with improvements of growth time, overall production yields, and bioactive qualities. © 2024 The Authors

关键词： anti-inflammation Antioxidant Flavonoid glycosides Houttuynia cordata Thunb. Hydroponics Plant growth-promoting additive

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Microbiota analysis in the hemodialysis population - Focusing on Enterobacteriaceae

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Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 2023年第2期56卷 311-323页

作者： Hon-Yen Wu Yi-Tsung Lin Wan-Chuan Tsai Yen-Ling Chiu Mei-Ju Ko Ju-Yeh Yang Mei-Fen Pai Hsin-Sui Hsu Shih-Ping Hsu Yu-Sen Peng Chun-Hsing Liao Department of Internal Medicine Far Eastern Memorial Hospital New Taipei City Taiwan School of Medicine College of Medicine National Yang Ming Chiao Tung University Taipei City Taiwan Institute of Epidemiology and Preventive Medicine College of Public Health National Taiwan University Taipei City Taiwan Department of Internal Medicine National Taiwan University Hospital and College of Medicine Taipei City Taiwan. Division of Infectious Diseases Department of Medicine Taipei Veterans General Hospital Taipei City Taiwan Institute of Emergency and Critical Care Medicine National Yang Ming Chiao Tung University Taipei City Taiwan. Center for General Education Lee-Ming Institute of Technology New Taipei City Taiwan. Graduate Program in Biomedical Informatics Yuan Ze University Taoyuan City Taiwan Graduate Institute of Medicine Yuan Ze University Taoyuan City Taiwan. Department of Dermatology Taipei City Hospital Taipei City Taiwan Department of Dermatology National Taiwan University Hospital and College of Medicine Taipei City Taiwan. Department of Internal Medicine Far Eastern Memorial Hospital New Taipei City Taiwan. School of Life Science National Taiwan Normal University Taipei City Taiwan. Department of Applied Cosmetology Lee-Ming Institute of Technology New Taipei City Taiwan Department of Healthcare Administration Asia Eastern University of Science and Technology New Taipei City Taiwan. School of Medicine College of Medicine National Yang Ming Chiao Tung University Taipei City Taiwan. Electronic address: liaoch@femh.org.tw.

BACKGROUND:Infection is a recognized risk factor for mortality among hemodialysis (HD) population, including infection caused by Enterobacteriaceae. We aimed to investigate Enterobacteriaceae in gut microbiota among HD patients and to analyze associations between microbiota and clinical parameters. METHODS:This prospective study of microbiota analysis in HD patients was conducted in April-May 2019. A control group without recent antibiotic use or hospitalization was used for comparison. Stool samples underwent 16S rRNA sequencing, using Greengenes 16S rRNA database for microbiota analysis. RESULTS:Among 96 hemodialysis (HD) patients, mean age was 61.9 ± 0.8 years and mean duration of HD was 6.5 ± 0.7 years. No significant differences were found in alpha diversity between HD and control groups (HD group 949.5, controls 898; p = 0.16) although significant between-group differences were found in beta diversity (p < 0.001). At phylum level, HD group had a higher abundance of Firmicutes and Proteobacteria, but lower abundance of Bacteriodetes. At genus level, Escherichia-Shigella complex increased among HD patients who had hospitalization with 1 year (median 0.024 vs 0.004, p = 0.054) and Klebsiella was associated with emergency room visit within 1 year among HD patients (p = 0.002). CONCLUSIONS:Alpha diversity in HD patients is not lower than that in healthy controls but significant between-group differences are found in microbiota composition according to beta diversity, due to decreased Bacteriodetes and increased Firmicutes and Proteobacteria. Deeper microbiota analyses for Enterobacteriaceae are necessary. Whether change in dietary components can help to decrease mortality among dialysis population warrants further research.

关键词： Chronic Enterobacteriaceae Gastrointestinal microbiome Hemodialysis Kidney failure Microbiota Prognosis

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Adjacent segment disease following Dynesys stabilization for lumbar disorders:A case series of mid-and long-term follow-ups

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World Journal of Clinical Cases 2021年第35期9卷 10850-10860页

作者： Kuan-Ju Chen Chien-Ying Lai Lu-Ting Chiu Wei-Sheng Huang Pang-Hsuan Hsiao Chien-Chun Chang Cheng-Jyh Lin Yuan-Shun Lo Yen-Jen Chen Hsien-Te Chen Department of Orthopedic Surgery China Medical University HospitalTaichung City 404Taiwan Spine Center China Medical University HospitalTaichung City 404Taiwan Management Office for Health Data China Medical University HospitalTaichung City 404Taiwan College of Medicine China Medical UniversityTaichung City 404Taiwan School of Chinese Medicine China Medical UniversityTaichung City 404Taiwan Department of Orthopedic Surgery China Medical University HospitalChina Medical UniversityTaichung City 404Taiwan Spine Center China Medical University HospitalChina Medical UniversityTaichung City 404Taiwan Degree Program of Biomedical Science and Engineering National Chiao Tung UniversityHsinchu City 300Taiwan Department of Biological Science and Technology National Chiao Tung UniversityHsinchu City 300Taiwan Department of Orthopedic Surgery China Medical University Beigang HospitalYunlin County 651Taiwan Graduate Institute of Precision Engineering National Chung Hsing UniversityTaichung City 404Taiwan School of Medicine China Medical UniversityTaichung City 404Taiwan Department of Sport Medicine College of Health CareChina Medical UniversityTaichung City 404Taiwan

BACKGROUND Radiologic adjacent segment degeneration(ASDeg)can occur after spinal *** segment disease(ASDis)is defined as the development of new clinical symptoms corresponding to radiographic changes adjacent to the level of previous spinal *** pre-existing ASDeg is generally considered to result in more severe ASDis;nonetheless,whether the ASDeg status before index surgery influences the postoperative risk of revision surgery due to ASDis warrants *** To identify possible risk factors for ASDis and verify the concept that greater preexisting ASDeg leads to more severe *** Data from 212 patients who underwent posterior decompression with Dynesys stabilization from January 2006 to June 2016 were retrospectively *** who underwent surgery for ASDis were categorized as group A(n=13),whereas those who did not were classified as group B(n=199).Survival analysis and Cox proportional hazards models were used to compare the modified Pfirrmann grade,University of California-Los Angeles grade,body mass index,number of Dynesys-instrumented levels,and *** The mean time of reoperation was 7.22(1.65–11.84)years in group A,and the mean follow-up period was 6.09(0.10–12.76)years in group *** significant difference in reoperation risk was observed:Modified Pfirrmann grade 3 vs 4(P=0.53)or 4 vs 5(P=0.46)for the upper adjacent disc,University of California-Los Angeles grade 2 vs 3 for the upper adjacent segment(P=0.66),age of<60 vs>60 years(P=0.9),body mass index<25 vs>25 kg/m2(P=0.3),and sex(P=0.8).CONCLUSION Greater preexisting upper ASDeg was not associated with a higher rate of reoperation for ASDis after Dynesys *** overweight tended to increase reoperation risk after Dynesys surgery for ASDis.

关键词： Adjacent segment degeneration Adjacent segment disease Degenerative lumbar spondylolisthesis Dynamic stabilization Dynesys Spinal stenosis

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Robustness tests for biomedical foundation models should tailor to specification

arXiv

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arXiv 2025年

作者： Xian, R. Patrick Baker, Noah R. David, Tom Cui, Qiming Holmgren, A. Jay Bauer, Stefan Sushil, Madhumita Abbasi-Asl, Reza Weill Institute for Neurosciences University of California San Francisco 1651 4th Street San FranciscoCA94158 United States Biological and Medical Informatics Graduate Program University of California San Francisco 550 16th Street 3rd Floor San FranciscoCA94158 United States PRISM Eval 10 Rue de Penthièvre Paris75008 France Department of Bioengineering University of California Berkeley 306 Stanley Hall University Drive BerkeleyCA94720 United States Division of Clinical Informatics and Digital Transformation University of California San Francisco 10 Koret Way San FranciscoCA94117 United States School of Computation Information and Technology Technical University of Munich & Helmholtz AI Arcisstrasse 21 Munich80333 Germany Bakar Computational Health Sciences Institute University of California San Francisco 490 Illinois Street San FranciscoCA94158 United States

Existing regulatory frameworks for biomedical AI include robustness as a key component but lack detailed implementational guidance. The recent rise of biomedical foundation models creates new hurdles in testing and certification given their broad capabilities and susceptibility to complex distribution shifts. To balance test feasibility and effectiveness, we suggest a priority-based, task-oriented approach to tailor robustness evaluation objectives to a predefined specification. We urge concrete policies to adopt a granular categorization of robustness concepts in the specification. Our approach promotes the standardization of risk assessment and monitoring, which guides technical developments and mitigation efforts. © 2025, CC BY-NC-SA.

关键词： Specifications

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Multiomics analysis reveals microbial diversity and activity through spontaneous fermentation of Theobroma cacao

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Heliyon 2024年第23期10卷 e40542页

作者： da Costa Fonseca, Ynara Bahule, Celina Eugenio Herrera, Hector da Silva Martins, Luiza Helena Lopes, Alessandra Santos Cassoli, Juliana Silva Trindade, Felipe Costa Chagas da Costa, Isa Rebecca Henrique de Oliveira Costa, Paulo Oliveira, Guilherme Borges da Silva Valadares, Rafael Graduate Program in Agricultural Applied Biotechnology Federal Rural University of Amazonia President Tancredo Neves Ave 2501 Belém CEP 66.077-830 Brazil Vale Institute of Technology Boaventura da Silva Street 955 Belém CEP 66050-090 Brazil Center of Studies in Science and Technology (NECET) Rovuma University Niassa Branch Lichinga Mozambique Departamento de Ciencias Forestales Facultad de Ciencias Agropecuarias y Medioambiente Universidad de La Frontera Temuco 4811230 Chile Center for Biodiversity and Ecological Sustainability (C-BEST) Facultad de Ciencias Agropecuarias y Medioambiente Universidad de La Frontera Chile Institute of Animal Health and Production Federal Rural University of Amazonia President Tancredo Neves Ave. 2501 Belém CEP 66077-830 Brazil Faculty of Food Engineering Institute of Technology Federal University of Pará Belém CEP 66075-110 Brazil Laboratory of Omics Sciences Institute of Biological Sciences Guamá Campus Federal University of Pará Belém 66075-110 Brazil

To gain insight into the active microbiota during spontaneous fermentation of Theobroma cacao L., this study assessed protein diversity during 120 h using a combined metabarconding and metaproteomics approach. During the first days of fermentation, most of the peptides were associated with T. cacao and yeast (0–72 h). Peptides associated with bacteria became more abundant after 72 h of fermentation, coinciding with a decrease in peptides associated with cocoa (96–120 h). In addition to the known microorganisms involved in fermentation, such as Saccharomyces, Lactobacillus and Acetobacter, novel genera were also metabolically active, including Microvirga, Inquilinus, Candolleomyces and Lasiodiplodia. The results showed a consistency in the main genera detected by both techniques, but the identification of unexplored genera such as Inquilinus, Microvirga, Cyphellophora and Ashbya gossypii, among others, suggests that this omics approach needs to be used together for more comprehensive results on spontaneous fermentation. In conclusion, studies combining techniques such as metabarcoding and metaproteomics should be considered in fermentation studies, as a single technique would result in omissions regarding the activity of certain microorganisms that may be important for the course of spontaneous fermentation. © 2024

关键词： Bacteria Cocoa Metabarcoding Metaproteomics

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Current advances in the brewery wastewater treatment from anaerobic digestion for biogas production: A systematic review

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Environmental Advances 2023年 13卷

作者： Maria, Mariana Pires Torres, Nádia Hortense Nascimento, Victor Ruan Silva Chagas, Talita Santos Alves Saratale, Ganesh Dattatraya Mulla, Sikandar I. Bharagava, Ram Naresh Cavalcanti, Eliane Bezerra Romanholo Ferreira, Luiz Fernando Graduate Program in Process Engineering Tiradentes University Av. Murilo Dantas 300 Farolândia SE Aracaju 49032-490 Brazil Institute of Technology and Research Av. Murilo Dantas 300 Farolândia SE Aracaju 49032-490 Brazil Department of Food Science and Biotechnology Dongguk University Ilsandong-gu Goyang-si Gyeonggi-do Seoul 10326 South Korea Department of Biochemistry School of Allied Health Sciences REVA University Bangalore India Laboratory of Bioremediation and Metagenomics Research (LBMR) Department of Environmental Microbiology (DEM) Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar Raebareli Road U.P. Lucknow 226 025 India

Brazil is the third largest beer producer in the world and has several brewing industries. These industries cover a range of production processes due to their wide variety of beverages, which leads to the generation of effluents with different physicochemical characteristics. Furthermore, these residues are usually disposed of without any treatment despite having enormous potential for biogas and bioenergy production. Biorefinery is a relevant alternative to the transition from a linear economy to a Circular Economy (CE). The Biorefinery exploits all available resources in the production of goods and contributes to sustainable development in the face of industrial production. In this context, as an alternative solution, anaerobic digestion (AD) has been commonly used as it uses different biomass sources to satisfy current energy demands. AD can be implemented to treat brewing effluent, promote its energy recovery, reduce environmental pollution, and produce environmentally friendly biological products: biogas and biofertilizer. Thus, this review explored the potential of AD for brewery waste management in association with the bio-based CE. Overall, the current manuscript emphasized the potential of brewery waste for biogas generation and energy production, discussing its adoption and challenges. © 2023

关键词： Anaerobic digestion Bioenergy Biogas Brewery wastewater

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The Water Quality Characteristics and Quality Status of Bengkulu River and Nelas River, Bengkulu Province: Conditions for The Last Six Years

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IOP Conference Series: Earth and Environmental Science 2022年第1期950卷

作者： I Khalik A Sapei S Hariyadi E Anggraeni Natural Resources and Environmental Management Study Program Graduate School of IPB University Bogor Indonesia Public Health Study Program STIKES Tri Mandiri Sakti Bengkulu Indonesia Department of Civil and Environmental Engineering IPB University Bogor Indonesia Department of Aquatic Resources Management IPB University Bogor Indonesia Department of Agroindustrial Technology IPB University Bogor Indonesia

The existence of extractive activities around the river has a negative impact on the quality of river water. This study aims to provide an overview of water quality characteristics and water quality status of Bengkulu River and Nelas River in Bengkulu Province. The data used is river quality monitoring conducted by the Environment and Forestry Service of Bengkulu Province from 2014 to 2019. Measurements are carried out twice a year representing, the dry season (March to June) and the rainy season (September to December). The observation stations representing upstream, middle and downstream of rivers conditions. The parameters observed were physical, chemical, and biological parameters. The water quality status is determined using the Storet Index. The results show that both rivers have Storet Index >-31. It means that these river water are heavily polluted. Parameters that have exceeded the water quality standard are TSS, DO, BOD, COD, Fe, Mn, Cu, and total coliform. The river water quality parameters, especially heavy metals, may be caused by open land being washed and carried into the river body. The existence of population and animal activities as well as industrial waste outlets at upstream also affect the decline of river water quality.

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Sustainable biorefinery with Chlorolobion braunii cultivated in produced water: Bioremediation integrated with the production of exopolysaccharides, biodiesel, and bio-oil

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Chemical Engineering Journal 2025年 518卷

作者： Jadson dos Santos França Ravena Maria de Almeida Medeiros Ingrid Rocha Teixeira Jânia Betânia Alves da Silva Denilson de Jesus Assis Carolina Oliveira de Souza Rosangela Regia Lima Vidal Bianca Bomfim Andrade Fernanda Teixeira Cruz José Geraldo Pacheco Roger Fréty Lucas Guimarães Cardoso Graduate Program in Chemical Engineering (PPEQ) Polytechnic School Federal University of Bahia (UFBA) Salvador Bahia Brazil Center of Science and Technology Mechanical Engineering Collegiate Federal University of Recôncavo of Bahia (UFRB) Cruz Das Almas Bahia Brazil School of Exact and Technological Sciences Salvador University Salvador Bahia Brazil Graduate Program in Food Sciences Faculty of Pharmacy Federal University of Bahia (UFBA) Salvador Bahia Brazil Department of Physical Chemistry Institute of Chemistry Federal University of Bahia (UFBA) Salvador Bahia Brazil Health Sciences Institute Federal University of Bahia (UFBA) Salvador Bahia Brazil Catalysis and Materials Laboratory Department of General and Inorganic Chemistry Institute of Chemistry Federal University of Bahia (UFBA) Salvador Bahia Brazil Federal University of Pernambuco (UFPE) Center of Technology and Geosciences Department of Chemical Engineering Institute for Petroleum and Energy Research (i-LITPEG) Laboratory of Refining and Cleaner Technology (Lateclim) Recife PE 50740-540 Brazil

Petroleum extraction generates large volumes of produced water (PW), which is an effluent containing high concentrations of pollutants that impact the environment and present a significant environmental liability for the fossil fuel industry. Among the various treatment approaches, biological methods—particularly those involving microalgae—have shown promise for PW remediation. In this study, we evaluated the bioremediation potential of PW using a microalga Chlorolobion braunii cultivated in 250 L photobioreactors. C. braunii was cultivated in a mixture containing 50 % PW and 50 % BG-11 (Blue green 11) medium (PW50%), while the control comprised only the BG-11 medium. Results demonstrated high removal efficiencies for nitrate (99.54 %), phosphate (77.78 %), iron (94 %), and manganese (75 %), along with a reduction in alkalinity (61 %). A total of 40 g of biomass was produced using 100 L of PW, containing 8.50 % lipids. This biomass mainly contained palmitic acid (C16:0) and elaidic acid (C18:1ω9t), indicating its suitability for biodiesel production according to the International Quality Standards of the American Society for Testing and Materials (ASTM D6751 ) and the Brazilian National Agency of Petroleum, Natural Gas, and Biofuels (ANP). The biomass also yielded 35.12 % bio-oil and 57.69 % non-condensable gases during pyrolysis. Furthermore, 30 g of exopolysaccharides (EPS) were obtained, which exhibited pseudoplastic characteristics—a property of industrial interest. Thus, the treatment using 50 % PW for the cultivation of C. braunii was deemed a promising strategy for bioremediation and facilitated the production of high-value bioproducts.

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Corrigendum to "Investigating nurses' acceptance of patients' bring your own device implementation in a clinical setting: A pilot study" [Asia-Pacific J Oncol Nurs 10 (2023) 100195]

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Asia-Pacific journal of oncology nursing 2024年第5期11卷 100437页

作者： Shuo-Chen Chien Chun-You Chen Chia-Hui Chien Usman Iqbal Hsuan-Chia Yang Huei-Chia Hsueh Shuen-Fu Weng Wen-Shan Jian Graduate Institute of Biomedical Informatics College of Medical Science and Technology Taipei Medical University Taipei Taiwan. Artificial Intelligence Research and Development Center Wan Fang Hospital Taipei Medical University Taipei Taiwan. International Center for Health Information and Technology College of Medical Science and Technology Taipei Medical University Taipei Taiwan. Department of Radiation Oncology Wan Fang Hospital Taipei Medical University Taipei Taiwan. Office of Public Affairs Taipei Medical University Taipei Taiwan. Health ICT Department of Health Tasmania Australia. Global Health and Health Security Department College of Public Health Taipei Medical University Taipei Taiwan. Department of Artificial Intelligence in Medicine Professional Master Program Taipei Medical University Taipei Taiwan. Department of Pharmacy Taipei Veterans General Hospital Taipei Taiwan. Division of Endocrinology and Metabolism Department of Internal Medicine Taipei Medical University Hospital Taipei Taiwan. Division of Endocrinology and Metabolism Department of Internal Medicine School of Medicine College of Medicine Taipei Medical University Taipei Taiwan. School of Health Care Administration Taipei Medical University Taipei Taiwan. School of Gerontology Health Management College of Nursing Taipei Medical University Taipei Taiwan. Research Center for Artificial Intelligence in Medicine Taipei Medical University Taipei Taiwan. Graduate Institute of Data Science Taipei Medical University Taipei Taiwan.

[This corrects the article DOI: 10.1016/***.2023.100195.].

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ORGANIZATION OF PRIMARY healthCARE FOR USERS WITH CHRONIC MUSCULOSKELETAL PAIN BY PROGNOSTIC STRATIFICATION

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Brazilian Journal of Physical Therapy 2024年 28卷 100914-100914页

作者： Baroni, Marina P Yocoyama, Karolaine de Medeiros Knaut, Sibele AM Umburanas, Rubia Caldas Clemente, Pedro Augusto Saragiotto, Bruno T Masters and Doctoral Programs in Physical Therapy Universidade Cidade de São Paulo (UNICID) São Paulo São Paulo Brazil Department of Physical Therapy Universidade Estadual do Centro-Oeste (UNICENTRO) Guarapuava Paraná Brazil Postgraduate Program in Pharmaceutical Sciences Universidade Estadual do Centro-Oeste (UNICENTRO) Guarapuava Parana Brasil Discipline of Physiotherapy Graduate School of Health University of Technology Sydney Sydney Australia

BackgroundPrimary healthcare provides coordinated care to the needs of users of the Brazilian Unified health System (SUS), within the health Care Network (RAS), in a collaborative intervention model that involves the primary, secondary and tertiary levels of healthcare. The prognostic stratification of SUS users with chronic musculoskeletal pain can help structure the care network and establish adequate flows for user care. ObjectivesTo analyze the prognostic stratification of SUS users with chronic musculoskeletal pain referred to the pain neuroscience education program ( EducaDor), in the city of Guarapuava, Brazil MethodsThis is an exploratory cross-sectional observational study, following the recommendations of Strengthening the Reporting of Observational Studies in Epidemiology (STROBE). The sample consisted of 140 SUS users with chronic musculoskeletal pain, referred from primary healthcare in Guarapuava city to the EducaDor program. The prognostic stratification for chronic musculoskeletal pain was analyzed using the Keele STarT MSK Tool: 0 to 4 points for low risk, 5 to 8 points for medium risk, and 9 to 12 points for high prognostic risk. Sociodemographic data, performance and location of physiotherapeutic treatment (primary healthcare, or medium-complexity clinic) were collected. ResultsThe study included 114 SUS users with chronic musculoskeletal pain, aged 55.46±11.9 years, and 79% (n=90) were women. 7% (n=8) of SUS users were low risk, 43% (n=49) were medium risk, and 50% (n=57) were high risk. It was also observed that primary healthcare did not carry out physical therapy interventions for users with low risk, intervening only in users with medium and high risk (n=31; 27.2%). ConclusionPrimary healthcare performs physical therapy interventions for SUS users with chronic musculoskeletal pain at medium and high prognostic risk. This indicates that an organizational redesign of the referral system for users with chronic musculoskeletal pain should be stru

关键词： Primary healthcare Chronic pain Prognosis

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