检索结果-内蒙古大学图书馆

Cover Picture: Acceleration of Calcite Kinetics by Abalone Nacre Proteins (Adv. Mater. 22/2005)

Advanced materials 2005年第22期17卷

作者： G. Fu S. R. Qiu C. A. Orme D. E. Morse J. J. De Yoreo Biomolecular Science and Engineering Graduate Program and the Institute for Collaborative Biotechnologies University of California at Santa Barbara Santa Barbara CA 93106 USA Department of Chemistry and Materials Science Lawrence Livermore National Laboratory P.O. Box 808 Livermore CA 94551 USA

Abalone utilizes a system of macromolecular matrices and soluble proteins to produce beautiful and mechanically robust shells. The cover shows work by Qiu and co‐workers reported on p. 2678, in which AP8 proteins isolated from the shell of red abalone are shown to alter the growth of calcite both by accelerating the rate and modifying the shape from the simple rhombohedra seen in the upper left of the scheme to the more complex form seem in the lower right. The changes are made manifest at an atomic scale through alterations in the growth speed and shape of the atomic steps that form the growth hillocks (background).

关键词： Biomineralization Calcination Proteins

来源：评论

学校读者我要写书评

暂无评论

Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes

引用

materials science and engineering C 2004年第3期24卷 425-429页

作者： Miyata, Shogo Furukawa, Katsuko S. Ushida, Takashi Nitta, Yasuo Tateishi, Tetsuya Biomedical Engineering Laboratory Dept. of Mechanical Enginieering Graduate School of Engineering Hongo 7-3-1 Bunkyo Tokyo 113-8656 Japan Biomaterials Center Natl. Inst. for Materials Science Japan

Recently, an approach to restore cartilage defects, culturing autologous chondrocytes in vitro to create a three-dimensional tissue and then implanting the cultured tissue, has been developed. In this kind of approach, it is important to study whether the extracellular matrixes synthesized by cultured chondrocytes have appropriate mechanical property. In this study, we attempted to evaluate in detail mechanical properties of the extracellular matrix synthesized by chondrocytes in vitro. Therefore, we cultured bovine chondrocytes in agarose gel and assessed the changes in not only static but also dynamic mechanical properties during long-term culture. In addition, to assess the mechanical property of the extracellular matrix synthesized by chondrocytes only, we calculated the volume ratio of the extracellular matrix in agarose gel from the cross section and estimated the static Young's modulus of the extracellular matrix synthesized by cultured chondrocytes based on the Rule of Mixture. As a result, both the equilibrium aggregate modulus and dynamic Young's modulus of chondrocyte/agarose disks increased with time. The value of calculated modulus was larger than that of the pericellular matrix of cartilage explant. In conclusion, the biomechanical property of chondrocyte/agarose constructs and the matrix synthesized by chondrocytes were assessed in detail. © 2004 Elsevier B.V. All rights reserved.

关键词： Cartilage

来源：评论

学校读者我要写书评

暂无评论

Effect of type I and type II collagen sponges as 3D scaffolds for hyaline cartilage-like tissue regeneration on phenotypic control of seeded chondrocytes in vitro

引用

materials science and engineering C 2004年第3期24卷 407-411页

作者： Ohno, Takahiro Tanisaka, Keizo Hiraoka, Yosuke Ushida, Takashi Tamaki, Tamotsu Tateishi, Tetsuya Department of Mechanical Engineering Nippon Institute of Technology Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Tokyo Japan Biochemical Laboratory Nitta Gelatin Inc. Japan Natl. Inst. for Materials Science Biomaterials Center Japan

Biodegradable polymers have been adopted as materials of three- dimensional (3D) scaffolds for regenerating cartilage-like tissues by using tissue engineering. On the other hand, it is well recognized that phenotypic control of seeded chondrocytes is indispensable for constructing not fibrous but hyaline cartilage-like tissue. According to the context, type II collagen is focused as a candidate of scaffold materials for articular cartilage regeneration. This study compared viability and gene expressions of seeded chondrocytes in type I and type II collagen sponges. The results show that the seeded chondrocytes were well distributed and alive in the both sponges in 20 days of culture, and no significant difference in type I, type II and aggrecan gene expressions was found between both sponges. The effect of type II collagen as a scaffold material having outside in-signals on regeneration of hyaline cartilage-like tissue was not detected at least at the initial stage of tissue regeneration. © 2004 Published by Elsevier B.V.

关键词： materials science

来源：评论

学校读者我要写书评

暂无评论

Aggregate formation of bone marrow stromal cells by rotation culture

引用

materials science and engineering C 2004年第3期24卷 421-424页

作者： Suenaga, Hideyuki Furukawa, Katsuko S Ushida, Takashi Takato, Tsuyoshi Tateishi, Tetsuya Dept. of Oral/Maxillofacial Surgery Graduate School of Medicine University of Tokyo 7-3-1 Hongo Bunkyo Tokyo 113-8655 Japan Biomedical Engineering Laboratory Department of Mechanical Engineering Graduate School of Engineering Tokyo Japan Biomaterials Center Natl. Inst. for Materials Science Ibaraki Japan

The rotation culture method adopted in our experiments induced numerous aggregates of bone marrow stromal cells (BMSCs);this method augments the frequency of cell-cell contacts and thereby facilitates cell aggregation. Cell condensation plays an important role in differentiation and development. Cell adhesion molecules and the extracellular matrix (ECM) are important regulators of cell adhesion and apoptosis. Therefore, we investigated not only whether aggregates of BMSCs could be formed by rotation culture, but also whether expression of N-cadherin and β1-integrin was involved in the formation of cell aggregates and in the avoidance of apoptosis. The culture medium was supplemented with fibroblast growth factor-2 (FGF-2) or transforming growth factor-β1 (TGF-β1), expected to prompt the formation of cell aggregates. This study enabled the formation of numerous aggregates of BMSCs by rotation culture. Reverse transcription- polymerase chain reaction (RT-PCR) analysis indicated that FGF-2 and TGF-β1 increased expression of N-cadherin and β1-integrin. Morphological analysis by light microscopy indicated that FGF-2 and TGF-β1 increased cell-cell adhesion and formation of cell aggregates. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay indicated that FGF-2 and TGF-β1 prevented apoptosis. These results suggest that numerous aggregates of BMSCs were formed efficiently by rotation culture since FGF-2 and TGF-β1 increase cell-cell adhesion and rotation increases cell-cell contact. © 2004 Published by Elsevier B.V.

关键词： Cells

来源：评论

学校读者我要写书评

暂无评论

Two-dimensional multi-array formation of hepatocyte spheroids on a micro-fabricated PEG-brush surface

Two-dimensional multi-array formation of hepatocyte spheroid...

引用

Transactions - 7th World biomaterials Congress

作者： Otsuka, H. Satomi, T. Itadani-Harada, J. Nagasaki, Y. Horiike, Y. Kataoka, K. Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Department of Materials Science Tokyo University of Science Noda Chiba Japan Dept. of Mat. Sci. and Engineering Graduate School of Engineering University of Tokyo Tokyo Japan

ISBN: (纸本)1877040193

The micropatterning of rat primary hepatocyte hetero-spheroids was prepared on microfabricated glass substrates coated with poly(ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes and endothelial cells was used to stabilize hepatocyte viability and liver-specific functions. Rat primary hepatocytes, suspended in a culture medium, were applied to the patterned dishes with cultured endothelial cells selectively located in the circular domains. Rat primary hepatocytes formed spheroids within 24-h only on the circular regions of existing endothelial cells, generating a two-dimensional (2D)-arrayed structure of the hepatocyte spheroids.

关键词： Cells

来源：评论

学校读者我要写书评

暂无评论

Construction of micro-patterned poly (ethylene glycol) brush surface for controlling protein and cell interactions

Construction of micro-patterned poly (ethylene glycol) brush...

引用

Transactions - 7th World biomaterials Congress

作者： Satomi, T. Otsuka, H. Nagasaki, Y. Horiike, Y. Kataoka, K. Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Department of Materials Science Tokyo University of Science Noda Chiba Japan Dept. of Mat. Sci. and Engineering Graduate School of Engineering University of Tokyo Tokyo Japan

ISBN: (纸本)1877040193

The micro-patterned poly(ethylene glycol) (PEG) brush surface for controlling protein and cell interactions was constructed. PEGylated substrates with two-dimensional arrays of plasma-etched circular domains were prepared by coating of mercapto-functionalized (PEG) on Au surface, followed by plasma-etching through a metal mask pattern with circular holes. The micro-patterning of bovine articular chondrocytes or rat primary hepatocytes hetero-spheroids underlaid with human umbilical endothelial cells (HUVEC) was achieved. These arrayed spheroids are promising materials for tissue and cell-based biosensors (TBB and CBB) as well as tissue engineering technologies.

关键词： Polyethylene glycols

来源：评论

学校读者我要写书评

暂无评论

Bone tissue engineering based on bead-cell sheets composed of calcium phosphate beads and bone marrow cells

引用

materials science and engineering C 2004年第3期24卷 437-440页

作者： Furukawa, Katsuko S. Miyauchi, Shunsuke Suzuki, Daisuke Umezu, Yoshikazu Shinjo, Tsuneo Ushida, Takashi Eguchi, Miki Tateishi, Tetsuya Biomed. Eng. Ushida/Furukawa Department of Mechanical Eng. Graduate School of Engineering 7-3-1 Hongo Bunkyo Tokyo 113-8656 Japan Advance Co. Saitama Japan Biomaterials Center Natl. Inst. for Materials Science Ibaraki Japan

We developed a new tissue-engineered bone graft model composed of bovine bone marrow stromal cells (BMSCs), and beta tricalcium phosphate (beta TCP) porous beads. Such cell-bead sheets were prepared by means of cultivation of BMSCs with the beads in alpha-MEM culture medium supplemented with 10% fetal bovine serum. The shape of the sheets was possible to change depending on that of a plastic mold having a circle or quadrangle shape. Addition of poundage on the overlaid cell-bead sheets induced the connection of these sheets with each other, and achievement of thicker implant formation. A nonwoven fabric sheet of poly-l-lactic acid (PLLA) could also be inserted in between each cell-bead sheet. Consequently, bone implants having a size of 20×20×5 mm were successfully developed. This kind of techniques would be useful in thick implants for other tissue implants. © 2004 Elsevier B.V. All rights reserved.

关键词： Bone

来源：评论

学校读者我要写书评

暂无评论

Heterotelechelic poly(ethylene glycol) tethered chain surface on gold for high-performance surface plasmon sensing

Heterotelechelic poly(ethylene glycol) tethered chain surfac...

引用

Transactions - 7th World biomaterials Congress

作者： Kato, M. Nagasaki, Y. Uchida, K. Takahashi, T. Kataoka, K. Preventure Program JST Department of Materials Science Tokyo University of Science 2641 Yamazaki Noda 278-8510 Japan Department of Materials Science Graduate School of Engineering University of Tokyo 7-3-1 Kongo Bunkyo-ku Tokyo 113-8656 Japan

ISBN: (纸本)1877040193

The specific sensing of antigen-antibody interaction was examined by using a PEGylated sensor. For the surface modification, acetal-PEG-SH was prepared and by using acetal-PEG-SH, PEG brush was constructed on the gold sensor chip surface. Modification of antibody was carried out by conjugation of SH group in Fab' to the maleimide group at the distal end of PEG chains on the surface. The result shows that the suitable modification of antibody on the PEG brush surface is very important to obtain high sensing character.

关键词： Polyethylene glycols

来源：评论

学校读者我要写书评

暂无评论

Improved synthesis with high yield and increased molecular weight of poly(α, β-malic acid) by direct polycondensation

Improved synthesis with high yield and increased molecular w...

引用

Transactions - 7th World biomaterials Congress

作者： Kajiyama, T. Kobayashi, H. Taguchi, T. Komatsu, Y. Kataoka, K. Tanaka, J. Biomaterials Center Natl. Institute of Materials Science Ibaraki Japan Graduate School of Engineering University of Tokyo Tokyo Japan Coll. of Environ. Eng. and Arch. Kanazawa Institute of Technology Ishikawa Japan

ISBN: (纸本)1877040193

The improved synthesis of poly(α,β-malic acid) (PMA) by direct polycondensation with high yield and increased molecular weight, was analyzed. To determine the chemical structure of the products, the 1NMR and 13C NMR spectra were also measured by an IR spectrometer. It was observed that the polymerization reaction proceeded faster at 130°C, but the molecular weight increased to about 3,500. The results show that the equilibrium of polymerization may shift toward the depolymerization at a higher reaction temperature, exhibiting a decrease in maximum molecular weight of α,β-PMA with an increase in the reaction temperature.

关键词： Carboxylic acids

来源：评论

学校读者我要写书评

暂无评论

New type of biodegradable porous scaffolds for tissue-engineered articular cartilage

引用

materials science and engineering C 2004年第3期24卷 431-435页

作者： Kawanishi, Makoto Ushida, Takashi Kaneko, Tadashi Niwa, Hideo Fukubayashi, Toru Nakamura, Kozo Oda, Hiromi Tanaka, Sakae Tateishi, Tetsuya Department of Orthopaedic Surgery Graduate School of Medicine University of Tokyo Tokyo Japan Biomedical Engineering Laboratory Department of Mechanical Engineering Graduate School of Engineering 7-3-1 Hongo Bunkyo Tokyo 113-8656 Japan Res./Devmt.Dept.Biomaterial Grp. GC Corporation Tokyo Japan Takasago Res. Lab. Life Sci. Ctr. Kaneka Corporation Hyogo Japan Department of Life Science Graduate School of Arts and Sciences University of Tokyo Tokyo Japan Biomaterials Center Natl. Inst. for Materials Science Ibaraki Japan

Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering. We created double-layered porous scaffolds composed of synthetic biodegradable polymers by combining freeze-drying and porogen leaching techniques to create tissue- engineered articular cartilage. The pore structures had two layers, a leaching side (upper) and a freeze-drying side (lower). The latter had columnar pores to simulate a radial zone, while the leaching side simulated the tangential zone of the articular cartilage. In this study, we showed that this structure enabled the uniform seeding of goat chondrocytes in the scaffolds, and with low rates of leakage. © 2004 Published by Elsevier B.V.

关键词： Cartilage

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：