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Transactions - 7th World biomaterials Congress

作者： Chen, G. Sato, T. Ohgushi, H. Ushida, T. Tateishi, T. Tissue Engineering Research Center Natl. Inst. Adv. Indust. Sci./Tech. Amagasaki Hyogo Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Bunkyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

ISBN: (纸本)1877040193

The application of hybrid mesh as a three-dimensional scaffold for the culture of canine articular chondrocytes and bone marrow stromal cell to generate osteochondral implants was discussed. The hybrid mesh was prepared by forming collagen microsponges in the openings of poly(lactic-co-glycolic acid) (PLGA) knitted mesh. It was found that both the chondrocytes and the stromal cells stick onto the hybrid scaffold and proliferated filling the spaces in the scaffold. The result show the possibility of construction of osteochondral implants by culturing chondrocytes and bone marrow stromal cells in the PLGA-collagen hybrid mesh.

关键词： Collagen

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Regeneration of cartilage tissue by using a hybrid mesh scaffold of biodegradable synthetic polymer PLGA and collagen

Regeneration of cartilage tissue by using a hybrid mesh scaf...

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Transactions - 7th World biomaterials Congress

作者： Chen, G. Sato, T. Ohgushi, H. Ushida, T. Tateishi, T. Tissue Engineering Research Center Natl. Inst. Adv. Indust. Sci./T. Amagasaki Hyogo Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Bunkyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

ISBN: (纸本)1877040193

The use of a hybrid mesh scaffold of biodegradable synthetic polymer PLGA and collagen for cartilage tissue engineering was discussed. The hybrid mesh was prepared by forming collagen microsponges in the opening of a PLGA knitted mesh made polylactine 910. The PLGA mesh and PLGA-collagen hybrid mesh structure were observed by scanning electron microscopy (SEM). Articular cartilage implant was reconstructed when bovine chondrocytes were cultured in the hybrid mesh. The bright safranin-O-positive stain indicated that glycosaminoglycans (GAG) were abundant and homogeneously distributed throughout the implants.

关键词： Cartilage

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Regeneration of cartilage tissue by combination of canine chondrocyte and a hybrid mesh scaffold

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materials science and engineering C 2004年第3期24卷 373-378页

作者： Chen, Guoping Sato, Takashi Ushida, Takashi Hirochika, Rei Ochiai, Naoyuki Tateishi, Tetsuya Tissue Engineering Research Center AIST Kansai Natl.Inst.Adv.Industr.Sci./Technol. 3-11-46 Nakoji Amagasaki Hyogo 661-0974 Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Japan Department of Orthopedics Institute of Clinical Medicine University of Tsukuba Tsukuba Japan Ctr.for DiseaseBiol./IntegrativeMed. Graduate School of Medicine University of Tokyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

Canine chondrocytes isolated from articular cartilage were cultured in a PLGA-collagen hybrid mesh in DMEM containing 10% FBS in vitro for 10 days, and subsequently implanted subcutaneously in the dorsum of nude mouse. The formation of neo-cartilage tissue was examined. HE staining showed a spherical morphology of chondrocytes in the center of the explants and a fibroblast-like morphology in the periphery of the explants. The safranin O/fast green staining showed that the central part was positively stained for GAG while the periphery was negatively stained. The toluidine blue staining showed a similar result. The histological results demonstrated spherical chondrocytes surrounded by abundant cartilaginous matrices in the center of the explants. The fibro-tissue in the periphery became denser and the central cartilage tissue became thinner as the implantation period increased, suggesting the effects such as invasion of host fibroblasts and diffusion of soluble factors on the degeneration of newly formed cartilage tissue. Gene expression study using reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that the expressions of type II collagen and aggrecan were detected and up-regulated, while that for type I collagen was down-regulated when the cells were cultured in the hybrid mesh. These results suggest the formation of articular cartilage in the subcutaneous dorsum of nude mouse. © 2004 Elsevier B.V. All rights reserved.

关键词： materials science

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Preparation of CdSe/ZnS quantum Dots-PEG-b-PAMA conjugate and application for immunohistochemistry

Preparation of CdSe/ZnS quantum Dots-PEG-b-PAMA conjugate an...

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Transactions - 7th World biomaterials Congress

作者： Nakamae, M. Takahashi, T. Ishii, T. Nagasaki, Y. Kataoka, K. Preventure Program JST Department of Materials Science Tokyo University of Science Yamazaki2641 Noda 278-8510 Japan Department of Matereals Science Graduate School of Engineering University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-8656 Japan

ISBN: (纸本)1877040193

The stable dispersion of quantum dot nanoparticles (QD) in aqueous solution using polymers containing poly(ethylene glycol) (PEG) segment was investigated. It was found that using PEG/poly(2-N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) enables stabilization of QD in aqueous phase. The photochemical characteristics of the PEG-modified QD were found to be almost same as unmodified QD. It was observed that as he PEG/PAMA block copolymer-modified QDs possess PEG tethered chains on the surface, it is highly dispersion stable in aqueous media and also showed non-fouling character.

关键词： Semiconductor quantum dots

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Extruded LZS glass-ceramics

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American Ceramic Society Bulletin 2004年第8期83卷

作者： Montedo, O.R.K. Reitz, G.M. Bertan, F.M. De Novaes Oliveira, A.P. Hotza, D. Siligardi, C. Graduate Program in Materials Science and Engineering Federal University of Santa Catarina Florianópolis Brazil Dept. of Materials and Environment Engineering University of Modena and Reggio Emilia Modena Italy

Experimental results regarding Li2O-ZrO2-SiO 2 (LZS) sintered glass ceramic materials obtained by extrusion of glass powders with an added inorganic material as binder, are presented. Each of the composition were characterized by chemical analysis, laser scattering particle-size analysis, differential thermal analysis (DTA), Xray diffractometry (XRD), and thermal expansion. It was observed that after a sintering process at near by 750°C, crystallization takes place as soon as the process gets over. Once heated, the the glass powder compacts crystallize into zircon and quartz and then to lithium disilicate.

关键词： Glass ceramics

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Basic fibroblast growth factor supports in vitro chondrogenesis of bone marrow-derived mesenchymal stem cells from patients with osteoarthritis

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materials science and engineering C 2004年第3期24卷 403-406页

作者： Akaogi, Hiroshi Akimoto, Takayuki Miyaki, Shigeru Ushida, Takashi Ochiai, Naoyuki Tateishi, Tetsuya Tanaka, Junzo Department of Orthopaedic Surgery Institute of Clinical Medicine University of Tsukuba Tennodai 1-1-1 Tsukuba Ibaraki 305-8575 Japan Biomaterials Center Natl. Inst. of Materials Science Namiki 1-1 Tsukuba Ibaraki 305-0044 Japan Department of Life Sciences Graduate School of Arts and Sciences The University of Tokyo Komaba 3-8-1 Meguro Tokyo 153-8902 Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Hongo 7-3-1 Bunkyo Tokyo 113-8656 Japan Biomaterial Center Natl. Inst. of Materials and Science Namiki 1-1 Tsukuba Ibaraki 305-0044 Japan

Bone marrow-derived mesenchymal stem cells (BMDMSCs) possess pluripotent differentiation potential including differentiation into osteoblasts, chondrocytes, adipocytes, and muscle cells. Using tissue engineering technology, the treatment of osteochondral lesions has been attempted with chondrocytes differentiated from mesenchymal stem cells in vitro. However, the capability of these stem cells for chondrogenic differentiation becomes gradually decreased with the passage of cell culture. Furthermore, previous studies showed that the cells from patients with osteoarthritis (OA) seemed to have reduced chondrogenic differentiation potential. It was reported that basic fibroblast growth factor (b-FGF) retained the multilinage differentiation potential of mesenchymal cells during expansion at the monolayer culture. In this study, we determined whether the BMDMSCs from patients with osteoarthritis could maintain their capability to differentiate into chondrocytes in the presence or absence of b-FGF in culture medium. We demonstrated that the chondrogenic differentiation capability of the BMDMSCs was improved by the addition of b-FGF. We concluded that bone marrow-derived stem cells from patients with osteoarthritis might be an acceptable cell source for cartilage tissue engineering. © 2004 Published by Elsevier B.V.

关键词： materials science

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The effect of coculture of chondrocytes with mesenchymal stem cells on their cartilaginous phenotype in vitro

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materials science and engineering C 2004年第3期24卷 391-396页

作者： Tsuchiya, Kohei Chen, Gouping Ushida, Takashi Matsuno, Takeo Tateishi, Tetsuya Department of Orthopedic Surgery Asahikawa Medical College Midorigaoka 2-1 Higashi Asahikawa 078-8510 Japan Biomaterials Center Natl. Inst. for Materials Science 1-2-1 Sengen Tsukuba Ibaraki 305-0047 Japan Tissue Engineering Research Center AIST Kansai Natl.Inst.Adv.Industr.Sci./Technol. 3-11-46 Nakoji Amagasaki Hyogo 661-0974 Japan Biomedical Engineering Laboratory Graduate School of Engineering The University of Tokyo Tokyo 113-8656 Japan

One of the major problems in autologous chondrocyte transplantation for cartilage defect repair is the acquisition of a sufficient number of chondrocytes. Chondrocytes lose their cartilaginous phenotype during expansion in monolayer culture. Bone marrow mesenchymal stem cells (MSCs) have the ability to differentiate into chondrocytes under appropriate conditions. In order to explore the feasibility of applying these cells simultaneously for cartilage tissue engineering, human MSC and bovine mature articular chondrocytes (BCH) were mixed and cocultured. Mixed pellet culture showed upregulation of cell proliferation, cartilaginous extracellular matrix production and type II collagen gene expression. MSCs mediated a paracrine effect on BCH through growth factor secretion or cell-to-cell interaction, upregulating the cartilaginous phenotype of BCH. © 2004 Elsevier B.V. All rights reserved.

关键词： Cell culture

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Anomalous binding profile of phenylboronic acid with N-acetylneuraminic acid (Neu5Ac) in aqueous solution with varying pH

Anomalous binding profile of phenylboronic acid with N-acety...

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Transactions - 7th World biomaterials Congress

作者： Otsuka, Hidenori Uchimura, Eiichiro Koshino, Hiroyuki Okano, Teruo Kataoka, Kazunori Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Dept. of Mat. Sci. and Engineering Graduate School of Engineering University of Tokyo Tokyo Japan Molecular Characterization Division RIKEN 2-1 Hirosawa Wako Saitama Japan Institute of Biomedical Engineering Tokyo Women's Medical University Shinjuku-ku Tokyo Japan

ISBN: (纸本)1877040193

The binding profile of 3-(propionomido) phenylboronic acid (PAPBA) with Neu5Ac was investigated using 1H, 13C, 15N, and 11B-NMR spectroscopies. PAPBA was prepared by the condensation reaction of propionic acid with 3-aminophenylboronic acid monohydrate in water at pH4.8, 0°C using [1-ethyl-3-(dimethyl-amino)propyl]carbodiimide hydrochloride (EDCHCl) as the condensation agent. The 11B NMR spectra were measured using a JEOL EX-400 fourier transform spectrometer operating at 128.15MHz for 11B.

关键词： Carboxylic acids

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Molecular recognition by stable dispersion of gold nanoparticles modified with heterobifunctional poly (ethylene glycol)

Molecular recognition by stable dispersion of gold nanoparti...

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Transactions - 7th World biomaterials Congress

作者： Aboshi, F. Takahashi, T. Ishii, T. Nagasaki, Y. Otsuka, Hidenori Kataoka, K. Pre-venture Program JST 4-1-8 Hon-machi Kawaguchi Saitama 332-0012 Japan Department of Materials Science Tokyo University of Science 2641 Yamazaki Noda Chiba 278-8510 Japan Department of Matereals Science Graduate School of Engineering University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-8656 Japan

ISBN: (纸本)1877040193

The stability of gold nanoparticles modified with heterobifunctional poly (ethylene glycol) (PEG) were investigated. An effective method for preparing hetero-bifunctional PEG by anionic ring-opening polymerization was also developed. Streptavidin-PEG gold nanoparticle showed extremely high response with biotinylated surface plasmon resonance (SPR) sensors. PEG-modified gold nanoparticles showed remarkable stability in physiological conditions, such as the solution of high ionic strength.

关键词： Nanostructured materials

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PH dependence of polypseudorotaxane formation between cationic linear polyethylenimine and cyclodextrins

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Macromolecules 2004年第18期37卷 6705-6710页

作者： Choi, Hak Soo Ooya, Tooru Lee, Sang Cheon Sasaki, Shintaro Kurisawa, Motoichi Uyama, Hiroshi Yui, Nobuhiko School of Materials Science Japan Adv. Inst. Sci. and Technol. 1-1 Asahidai Tatsunokuchi Ishikawa 923-1292 Japan Inst. of Bioeng. and Nanotechnology Singapore Science Park II #01-01/10 The Aries 117586 Singapore Department of Materials Chemistry Graduate School of Engineering Osaka University Suita Osaka 565-0871 Japan 21st Century COE Program Japan Adv. Inst. Sci. and Technol. 1-1 Asahidai Tatsunokuchi Ishikawa 923-1292 Japan

A series of linear polyethylenimines (LPEIs) with various molecular weights were synthesized and used for pH-dependent polypseudorotaxane formation with α- or γ-cyclodextrins (α-, γ-CDs). The polypseudorotaxane formation was significantly dependent on the pH of the aqueous media. The maximum yield of the recovered polypseudorotaxane precipitates was observed at pH 11.0, whereas no complexation was observed in the pH range below 8.0 due to the protonation of secondary amine groups in LPEI backbones. This suggests that the ionization of the secondary amine groups leads to dethreading of CD molecules because PEI chains with cationic nature disfavor the hydrophobic cavities of CDs. The solid-state 13C CP/MAS NMR and X-ray diffraction measurements verified the successful formation of crystalline polypseudorotaxanes through the inclusion complexation. In addition, 1H NMR analysis showed that LPEI formed a 2:1 inclusion complex with α-CD ([EI]:[α-CD] = 2:1) and a 4:1 complex with γ-CD ([EI]:[γ-CD] = 4:1) as reported in the poly(ethylene glycol)-based polypseudorotaxane system. Such unique pH-controllable polypseudorotaxane formation may be useful in designing many building blocks for stimuli-responsive polyrotaxanes.

关键词： Polyethylenes

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