检索结果-内蒙古大学图书馆

Bone tissue engineering based on bead-cell sheets composed of calcium phosphate beads and bone marrow cells

materials science and engineering C 2004年第3期24卷 437-440页

作者： Furukawa, Katsuko S. Miyauchi, Shunsuke Suzuki, Daisuke Umezu, Yoshikazu Shinjo, Tsuneo Ushida, Takashi Eguchi, Miki Tateishi, Tetsuya Biomed. Eng. Ushida/Furukawa Department of Mechanical Eng. Graduate School of Engineering 7-3-1 Hongo Bunkyo Tokyo 113-8656 Japan Advance Co. Saitama Japan Biomaterials Center Natl. Inst. for Materials Science Ibaraki Japan

We developed a new tissue-engineered bone graft model composed of bovine bone marrow stromal cells (BMSCs), and beta tricalcium phosphate (beta TCP) porous beads. Such cell-bead sheets were prepared by means of cultivation of BMSCs with the beads in alpha-MEM culture medium supplemented with 10% fetal bovine serum. The shape of the sheets was possible to change depending on that of a plastic mold having a circle or quadrangle shape. Addition of poundage on the overlaid cell-bead sheets induced the connection of these sheets with each other, and achievement of thicker implant formation. A nonwoven fabric sheet of poly-l-lactic acid (PLLA) could also be inserted in between each cell-bead sheet. Consequently, bone implants having a size of 20×20×5 mm were successfully developed. This kind of techniques would be useful in thick implants for other tissue implants. © 2004 Elsevier B.V. All rights reserved.

关键词： Bone

来源：评论

学校读者我要写书评

暂无评论

Heterotelechelic poly(ethylene glycol) tethered chain surface on gold for high-performance surface plasmon sensing

Heterotelechelic poly(ethylene glycol) tethered chain surfac...

引用

Transactions - 7th World biomaterials Congress

作者： Kato, M. Nagasaki, Y. Uchida, K. Takahashi, T. Kataoka, K. Preventure Program JST Department of Materials Science Tokyo University of Science 2641 Yamazaki Noda 278-8510 Japan Department of Materials Science Graduate School of Engineering University of Tokyo 7-3-1 Kongo Bunkyo-ku Tokyo 113-8656 Japan

ISBN: (纸本)1877040193

The specific sensing of antigen-antibody interaction was examined by using a PEGylated sensor. For the surface modification, acetal-PEG-SH was prepared and by using acetal-PEG-SH, PEG brush was constructed on the gold sensor chip surface. Modification of antibody was carried out by conjugation of SH group in Fab' to the maleimide group at the distal end of PEG chains on the surface. The result shows that the suitable modification of antibody on the PEG brush surface is very important to obtain high sensing character.

关键词： Polyethylene glycols

来源：评论

学校读者我要写书评

暂无评论

Improved synthesis with high yield and increased molecular weight of poly(α, β-malic acid) by direct polycondensation

Improved synthesis with high yield and increased molecular w...

引用

Transactions - 7th World biomaterials Congress

作者： Kajiyama, T. Kobayashi, H. Taguchi, T. Komatsu, Y. Kataoka, K. Tanaka, J. Biomaterials Center Natl. Institute of Materials Science Ibaraki Japan Graduate School of Engineering University of Tokyo Tokyo Japan Coll. of Environ. Eng. and Arch. Kanazawa Institute of Technology Ishikawa Japan

ISBN: (纸本)1877040193

The improved synthesis of poly(α,β-malic acid) (PMA) by direct polycondensation with high yield and increased molecular weight, was analyzed. To determine the chemical structure of the products, the 1NMR and 13C NMR spectra were also measured by an IR spectrometer. It was observed that the polymerization reaction proceeded faster at 130°C, but the molecular weight increased to about 3,500. The results show that the equilibrium of polymerization may shift toward the depolymerization at a higher reaction temperature, exhibiting a decrease in maximum molecular weight of α,β-PMA with an increase in the reaction temperature.

关键词： Carboxylic acids

来源：评论

学校读者我要写书评

暂无评论

New type of biodegradable porous scaffolds for tissue-engineered articular cartilage

引用

materials science and engineering C 2004年第3期24卷 431-435页

作者： Kawanishi, Makoto Ushida, Takashi Kaneko, Tadashi Niwa, Hideo Fukubayashi, Toru Nakamura, Kozo Oda, Hiromi Tanaka, Sakae Tateishi, Tetsuya Department of Orthopaedic Surgery Graduate School of Medicine University of Tokyo Tokyo Japan Biomedical Engineering Laboratory Department of Mechanical Engineering Graduate School of Engineering 7-3-1 Hongo Bunkyo Tokyo 113-8656 Japan Res./Devmt.Dept.Biomaterial Grp. GC Corporation Tokyo Japan Takasago Res. Lab. Life Sci. Ctr. Kaneka Corporation Hyogo Japan Department of Life Science Graduate School of Arts and Sciences University of Tokyo Tokyo Japan Biomaterials Center Natl. Inst. for Materials Science Ibaraki Japan

Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering. We created double-layered porous scaffolds composed of synthetic biodegradable polymers by combining freeze-drying and porogen leaching techniques to create tissue- engineered articular cartilage. The pore structures had two layers, a leaching side (upper) and a freeze-drying side (lower). The latter had columnar pores to simulate a radial zone, while the leaching side simulated the tangential zone of the articular cartilage. In this study, we showed that this structure enabled the uniform seeding of goat chondrocytes in the scaffolds, and with low rates of leakage. © 2004 Published by Elsevier B.V.

关键词： Cartilage

来源：评论

学校读者我要写书评

暂无评论

Application of PLGA-collagen hybrid mesh for the construction of osteochondral implants

Application of PLGA-collagen hybrid mesh for the constructio...

引用

Transactions - 7th World biomaterials Congress

作者： Chen, G. Sato, T. Ohgushi, H. Ushida, T. Tateishi, T. Tissue Engineering Research Center Natl. Inst. Adv. Indust. Sci./Tech. Amagasaki Hyogo Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Bunkyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

ISBN: (纸本)1877040193

The application of hybrid mesh as a three-dimensional scaffold for the culture of canine articular chondrocytes and bone marrow stromal cell to generate osteochondral implants was discussed. The hybrid mesh was prepared by forming collagen microsponges in the openings of poly(lactic-co-glycolic acid) (PLGA) knitted mesh. It was found that both the chondrocytes and the stromal cells stick onto the hybrid scaffold and proliferated filling the spaces in the scaffold. The result show the possibility of construction of osteochondral implants by culturing chondrocytes and bone marrow stromal cells in the PLGA-collagen hybrid mesh.

关键词： Collagen

来源：评论

学校读者我要写书评

暂无评论

Regeneration of cartilage tissue by using a hybrid mesh scaffold of biodegradable synthetic polymer PLGA and collagen

Regeneration of cartilage tissue by using a hybrid mesh scaf...

引用

Transactions - 7th World biomaterials Congress

作者： Chen, G. Sato, T. Ohgushi, H. Ushida, T. Tateishi, T. Tissue Engineering Research Center Natl. Inst. Adv. Indust. Sci./T. Amagasaki Hyogo Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Ibaraki Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Bunkyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

ISBN: (纸本)1877040193

The use of a hybrid mesh scaffold of biodegradable synthetic polymer PLGA and collagen for cartilage tissue engineering was discussed. The hybrid mesh was prepared by forming collagen microsponges in the opening of a PLGA knitted mesh made polylactine 910. The PLGA mesh and PLGA-collagen hybrid mesh structure were observed by scanning electron microscopy (SEM). Articular cartilage implant was reconstructed when bovine chondrocytes were cultured in the hybrid mesh. The bright safranin-O-positive stain indicated that glycosaminoglycans (GAG) were abundant and homogeneously distributed throughout the implants.

关键词： Cartilage

来源：评论

学校读者我要写书评

暂无评论

Regeneration of cartilage tissue by combination of canine chondrocyte and a hybrid mesh scaffold

引用

materials science and engineering C 2004年第3期24卷 373-378页

作者： Chen, Guoping Sato, Takashi Ushida, Takashi Hirochika, Rei Ochiai, Naoyuki Tateishi, Tetsuya Tissue Engineering Research Center AIST Kansai Natl.Inst.Adv.Industr.Sci./Technol. 3-11-46 Nakoji Amagasaki Hyogo 661-0974 Japan Biomaterials Center Natl. Inst. for Materials Science Tsukuba Japan Department of Orthopedics Institute of Clinical Medicine University of Tsukuba Tsukuba Japan Ctr.for DiseaseBiol./IntegrativeMed. Graduate School of Medicine University of Tokyo Tokyo Japan School of Science and Engineering Tokyo Denki University Saitama Japan

Canine chondrocytes isolated from articular cartilage were cultured in a PLGA-collagen hybrid mesh in DMEM containing 10% FBS in vitro for 10 days, and subsequently implanted subcutaneously in the dorsum of nude mouse. The formation of neo-cartilage tissue was examined. HE staining showed a spherical morphology of chondrocytes in the center of the explants and a fibroblast-like morphology in the periphery of the explants. The safranin O/fast green staining showed that the central part was positively stained for GAG while the periphery was negatively stained. The toluidine blue staining showed a similar result. The histological results demonstrated spherical chondrocytes surrounded by abundant cartilaginous matrices in the center of the explants. The fibro-tissue in the periphery became denser and the central cartilage tissue became thinner as the implantation period increased, suggesting the effects such as invasion of host fibroblasts and diffusion of soluble factors on the degeneration of newly formed cartilage tissue. Gene expression study using reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that the expressions of type II collagen and aggrecan were detected and up-regulated, while that for type I collagen was down-regulated when the cells were cultured in the hybrid mesh. These results suggest the formation of articular cartilage in the subcutaneous dorsum of nude mouse. © 2004 Elsevier B.V. All rights reserved.

关键词： materials science

来源：评论

学校读者我要写书评

暂无评论

Preparation of CdSe/ZnS quantum Dots-PEG-b-PAMA conjugate and application for immunohistochemistry

Preparation of CdSe/ZnS quantum Dots-PEG-b-PAMA conjugate an...

引用

Transactions - 7th World biomaterials Congress

作者： Nakamae, M. Takahashi, T. Ishii, T. Nagasaki, Y. Kataoka, K. Preventure Program JST Department of Materials Science Tokyo University of Science Yamazaki2641 Noda 278-8510 Japan Department of Matereals Science Graduate School of Engineering University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-8656 Japan

ISBN: (纸本)1877040193

The stable dispersion of quantum dot nanoparticles (QD) in aqueous solution using polymers containing poly(ethylene glycol) (PEG) segment was investigated. It was found that using PEG/poly(2-N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) enables stabilization of QD in aqueous phase. The photochemical characteristics of the PEG-modified QD were found to be almost same as unmodified QD. It was observed that as he PEG/PAMA block copolymer-modified QDs possess PEG tethered chains on the surface, it is highly dispersion stable in aqueous media and also showed non-fouling character.

关键词： Semiconductor quantum dots

来源：评论

学校读者我要写书评

暂无评论

Extruded LZS glass-ceramics

引用

American Ceramic Society Bulletin 2004年第8期83卷

作者： Montedo, O.R.K. Reitz, G.M. Bertan, F.M. De Novaes Oliveira, A.P. Hotza, D. Siligardi, C. Graduate Program in Materials Science and Engineering Federal University of Santa Catarina Florianópolis Brazil Dept. of Materials and Environment Engineering University of Modena and Reggio Emilia Modena Italy

Experimental results regarding Li2O-ZrO2-SiO 2 (LZS) sintered glass ceramic materials obtained by extrusion of glass powders with an added inorganic material as binder, are presented. Each of the composition were characterized by chemical analysis, laser scattering particle-size analysis, differential thermal analysis (DTA), Xray diffractometry (XRD), and thermal expansion. It was observed that after a sintering process at near by 750°C, crystallization takes place as soon as the process gets over. Once heated, the the glass powder compacts crystallize into zircon and quartz and then to lithium disilicate.

关键词： Glass ceramics

来源：评论

学校读者我要写书评

暂无评论

Basic fibroblast growth factor supports in vitro chondrogenesis of bone marrow-derived mesenchymal stem cells from patients with osteoarthritis

引用

materials science and engineering C 2004年第3期24卷 403-406页

作者： Akaogi, Hiroshi Akimoto, Takayuki Miyaki, Shigeru Ushida, Takashi Ochiai, Naoyuki Tateishi, Tetsuya Tanaka, Junzo Department of Orthopaedic Surgery Institute of Clinical Medicine University of Tsukuba Tennodai 1-1-1 Tsukuba Ibaraki 305-8575 Japan Biomaterials Center Natl. Inst. of Materials Science Namiki 1-1 Tsukuba Ibaraki 305-0044 Japan Department of Life Sciences Graduate School of Arts and Sciences The University of Tokyo Komaba 3-8-1 Meguro Tokyo 153-8902 Japan Biomedical Engineering Laboratory Graduate School of Engineering University of Tokyo Hongo 7-3-1 Bunkyo Tokyo 113-8656 Japan Biomaterial Center Natl. Inst. of Materials and Science Namiki 1-1 Tsukuba Ibaraki 305-0044 Japan

Bone marrow-derived mesenchymal stem cells (BMDMSCs) possess pluripotent differentiation potential including differentiation into osteoblasts, chondrocytes, adipocytes, and muscle cells. Using tissue engineering technology, the treatment of osteochondral lesions has been attempted with chondrocytes differentiated from mesenchymal stem cells in vitro. However, the capability of these stem cells for chondrogenic differentiation becomes gradually decreased with the passage of cell culture. Furthermore, previous studies showed that the cells from patients with osteoarthritis (OA) seemed to have reduced chondrogenic differentiation potential. It was reported that basic fibroblast growth factor (b-FGF) retained the multilinage differentiation potential of mesenchymal cells during expansion at the monolayer culture. In this study, we determined whether the BMDMSCs from patients with osteoarthritis could maintain their capability to differentiate into chondrocytes in the presence or absence of b-FGF in culture medium. We demonstrated that the chondrogenic differentiation capability of the BMDMSCs was improved by the addition of b-FGF. We concluded that bone marrow-derived stem cells from patients with osteoarthritis might be an acceptable cell source for cartilage tissue engineering. © 2004 Published by Elsevier B.V.

关键词： materials science

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：