This paper deals with the computation of ground resistance, surface voltage, touch voltage and step voltage, to mesh with horizontal wires arranged in different angles. The computer program implemented used in the mat...
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Organizations face an urgent need to become faster, more efficient, and more successful in their commercial operations, given the current state of technological evolution and the arrival of Industry 4.0. Many business...
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We recently proposed the novel gene-regulation polymer responding to cAMP-dependent protein kinase (PKA). However this polymer alone only had poor ability for the gene delivery into living cells. Thus it is difficult ...
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We recently proposed the novel gene-regulation polymer responding to cAMP-dependent protein kinase (PKA). However this polymer alone only had poor ability for the gene delivery into living cells. Thus it is difficult to evaluate the function of this polymer in living cells. On the other hand, it has been reported Arg-Gly-Asp (RGD) peptides modified polymer was efficiently deliveried genes into cells. In the present study, we modified the signal responsive polymer to RGD peptide for the introduction of polymer-DNA complex into living cells.
We recently proposed an artificial gene regulation systems responding to the intracellular protease signal, such as Caspase signal, which is the key signal of the cell death. For this purpose, we designed polymer-pept...
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We recently proposed an artificial gene regulation systems responding to the intracellular protease signal, such as Caspase signal, which is the key signal of the cell death. For this purpose, we designed polymer-peptide conjugate for a gene carrier. This polymer interacts with DNA through an electrostatic interaction and suppressed the gene-transcription. On the other hand, in the presence of active Caspase, this complex is disintegrated and the gene transcription is recovered. In this report, we applied this system to the cell free system and cultured cell, and succeeded in regulating the delivered gene expression responding to the intracellular protease signal.
We propose a novel method for gene therapy using drug or gene delivery system based on responses to intracellular signals, especially to the phosphorylation of protein kinase C (PKC) alpha. A cationic peptide that has...
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We propose a novel method for gene therapy using drug or gene delivery system based on responses to intracellular signals, especially to the phosphorylation of protein kinase C (PKC) alpha. A cationic peptide that has high phosphoarylation sensitivity for PKC alpha was designed, and then a polymer with these peptides was synthesized. In the electrophoresis examination, the polymer containing the cationic peptide formed a stable complex with anionic DNA, but free DNA was found after phosphorylation by PKC alpha. In the intratumor delivery of polymer/DNA complex, the luciferase activity in the positive polymer for PKC alpha at charge ratios of 0.1 and 0.3 was 10- to 20-fold higher than that in the negative control polymer with a peptide which the phosphorylated site Serine was substituted by Alanine. These results indicate that the D-RECS is applicable to gene therapy in vivo.
We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Sinc...
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We recently proposed the novel gene-regulation system responding to cAMP-dependent protein kinase (PKA). For this system, we used the cationic polymer which includes the oligopeptide that is the substrate of PKA. Since this polymer forms complexes with DNA, the gene expression was totally suppressed. However, when PKA is being continuously activated, anions were inserted in the polymer by phospholilating the peptides of side chains, the complex of DNA and polymer collapses and transcription of the gene is activated. We attempted to apply this system to living cell, and were successful to intracellular target signal-responsive regulation of delivered gene-expression.
作者：
Rabiha, Suciana GhadatiWibowo, AntoniLukasHeryadi, YayaComputer Science Department
BINUS Graduate Program-Doctor of Computer Science. Information Systems Department BINUS Online Learning Bina Nusantara University Jakarta11480 Indonesia Computer Science Department
BINUS Graduate Program-Doctor of Computer Science Bina Nusantara University 11480 Indonesia
Faculty of Engineering Universitas Katolik Indonesia Atma Jaya Indonesia Computer Science Department
BINUS Graduate Program - Doctor of Computer Science Bina Nusantara University 11480 Indonesia
One of the health problems that require special attention is diabetes, besides the growth of this disease infection is increasing in various circles ranging from children, adults, men, women and the elderly. So to det...
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A wide range of applications in the field of the Internet-of-Things (IoT) possess only strongly limited energy resources. This rises the need for Ultra-Low Power (ULP) designs that enable processing with lowest energy...
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Anonymity can protect a user's privacy, however it is hard to manage. It is hard to disclose anonymously. In addition, it is also hard to make people trust anonymous information. In this paper, we provide a key ex...
ISBN:
(纸本)1581139551
Anonymity can protect a user's privacy, however it is hard to manage. It is hard to disclose anonymously. In addition, it is also hard to make people trust anonymous information. In this paper, we provide a key exchange scheme for not only anonymous, but also traceable disclosure. To be more precise, one who provides information can anonymously guarantee communication with particular person. Furthermore, his anonymity can be retained even if he discloses communication contents. These features can not realized by using signature scheme, or any other existing methods. Our method enable those features, and it can be useful when a group is dealing with confidential information. Copyright 2004 ACM.
In this paper we report on the evolution of Apache Ant, a build automation tool developed in Java. We observed a typical case of architectural mismatch in this system: its original simple design was lost due to mainte...
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