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Title Biodecolorization of Biogas Power Plant Effluent Derived from Anaerobically Digested Distillery Wastewater by Natural Selected Pseudomonas Putida from Activated Sludge of the Wastewater Treatment Plant

SSRN

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SSRN 2023年

作者： Kumkaew, Ponruedee Suaisom, Pitchaya Mukkata, Kanokwan Koonaphapdeelert, Sirichai Sawatdeenarunat, Chayanon Nitayavardhana, Saoharit Master’s Degree Program in Environmental Engineering Faculty of Engineering Chiang Mai University Chiang Mai50200 Thailand Graduate School Chiang Mai University Chiang Mai50200 Thailand Energy Research and Development Institute – Nakornping Chiang Mai University Chiang Mai50200 Thailand Department of Environmental Engineering Faculty of Engineering Chiang Mai University Chiang Mai50200 Thailand Asian Development College for Community Economy and Technology Chiang Mai Rajabhat University Chiang Mai50300 Thailand Center of Excellence in Materials Science and Technology Chiang Mai University Chiang Mai50200 Thailand

The main aim of this study was to utilize naturally selected Pseudomonas putida strains isolated from activated sludge in a wastewater treatment plant, for the purpose of bioremediation of biogas effluent to remove organic content and color. Biogas effluent from anaerobically digestion of distillery wastewater treated with P. putida at the best evaluated condition (sample concentration of 50% v/v, agitation and aeration rates of 250 rpm and 1.0 vvm for 6 days) showed significant decolorization and organic removal efficacies of 34.29 ± 0.87% and 38.94 ± 0.63%, respectively. Bioremediation efficiency was dependent on bacterial growth which organic content in sample and dissolved oxygen during cultivation were key factors. The natural selected bacterium could tolerate higher organic content and work synergistically with other indigenous microorganisms found in the biogas effluent. Consequently, bioremediation of biogas effluent by natural selected P. putida is feasible to apply in biogas power plant where sterilization is not necessary in the process. © 2023, The Authors. All rights reserved.

关键词： Bioremediation

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Mechanism of Huayu Qutan recipe (化瘀祛痰方) anti-atherosclerosis mediates lipophagy via mammalian target of rapamycin complex 1/transcription factor EB signaling pathway in ApoE-/-mice

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Journal of Traditional Chinese Medicine 2025年第2期45卷 291-302页

作者： LI Yue PAN Jiaxiang YANG Guanlin YU Jiajia WU Xize MIN Dongyu CHENG Meijia YU Dongdong NAN Minghua GAO Xiaoyu PANG Linlin GONG Lihong JIA Lianqun Department of Cardiology the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Liaoning Provincial Key Laboratory of TCM Geriatric Cardio-Cerebrovascular Diseases Innovation Engineering Technology Center of Traditional Chinese Medicine Liaoning University of Traditional Chinese Medicine Postdoctoral Program of Liaoning University of Traditional Chinese Medicine Graduate School of Liaoning University of Traditional Chinese Medicine Experimental Center of Traditional Chinese Medicine the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Department of Osteology Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications of Liaoning University of Traditional Chinese Medicine Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Department of Oncology department Shengjing Hospital affiliated to China Medical University

OBJECTIVE: To investigate the effects of Huayu Qutan recipe(化瘀祛痰方, HYQT) on the atherosclerosis(AS) model of ApoE-/-mice with a high-fat diet and to illustrate the underlying mechanisms from modern pathophysiological conceptualizations. METHODS: High performance liquid chromatography of quadrupole time of flight-tandem mass spectrometry(HPLC-Q-TOF-MS/MS) analysis was used to identify the active compounds in the recipe. The mice were randomly allocated into 7 groups: control(CTRL) group, normal diet(ND) group, high-fat diet(HFD) group, HYQT groups(low dose, medium dose, and high dose), and simvastatin(SIM) group. Deferent doses of HYQT were gavaged twice a day, and then the protective effect of HYQT on plaque formation in ApoE-/-mice with a high-fat diet was verified via hematoxylin-eosin(HE) staining and oil red o(ORO) staining. We observed the co-localization in aortic macrophages and lipid droplets(LDs) by CD68 and the Bodipy fluorescence probe. Light chain 3 phosphoprotein class Ⅱ/light chain 3 phosphoprotein class Ⅰ(LC3Ⅱ/LC3 Ⅰ) was examined by western blotting, and sequestosome 1(SQSTM1/p62), Beclin1, Lamp1, mammalian target of rapamycin(mTOR), phosphorylated mammalian target of rapamycin(p-mTOR), and ATPbinding cassette transporter A1(ABCA1) were examined by real-time polymerase chain reaction(RT-PCR) and Western blotting. Transcription factor EB(TFEB) nuclear translocation was determined by immunofluorescence analysis. RESULTS: Five active compounds were identified using HPLC-Q-TOF-MS/MS analysis: ferulic acid, chlorogenic acid, calycosin, formononetin, and 8,2'-dihydroxy-7,4'-dimethoxy-isoflavane. The effect of HYQT on atherosclerotic plaque formation in Apo E-/-mice was investigated. These findings showed that HYQT decreased the co-localization of CD68 and Bodipy and increased the co-localization of CD68 and LC3B. Medium and high doses of HYQT increased autophagosome formation and promoted the maturation of LC3Ⅱ/LC3Ⅰ. Additionally, HYQT decreased the express

关键词： Huayu Qutan recipeSupporting information atherosclerosis diet, high-fat foam cells lipid metabolism

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IoT for Water Quality Categorization

IoT for Water Quality Categorization

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International Conference on Cybernetics and Intelligent System (ICORIS)

作者： Hermantoro Sastrohartono Andreas Wahyu Krisdiarto Arief Ika Uktoro Reza Rahutomo Teddy Suparyanto Bens Pardamean Agricultural Engineering Department Faculty of Agricultural Technology Institute of Agriculture STIPER Yogyakarta Indonesia Information System Department School of Information Systems Bina Nusantara University Jakarta Indonesia Bioinformatics & Data Science Research Center Bina Nusantara University Jakarta Indonesia Computer Science Department BINUS Graduate Program Master of Computer Science Bina Nusantara University Jakarta Indonesia

In agricultural water research, the adoption of Internet of Things (IoT) technology has emerged as a pivotal approach for large-scale data collection. Water availability in the context of water quality is very important, both for domestic and industrial purposes. For domestic purposes, drinking water and bathing water are separated. Meanwhile, for the palm oil industry, boiler filler is differentiated from additional process water (dilution water). Water quality parameters can be assessed from turbidity and Total Dissolve Solid (TDS). Measurements using measuring instruments separately and repeatedly require significant energy, time, and costs. This research was conducted with the primary objective of presenting a novel method for categorizing water quality with the approach of IoT sensor technology. The research methodology entailed the utilization of an integrated IoT water sensors system in conjunction with manual water categorization. The methods consist of (1) system design, (2) design and installation of sensor and IoT-based microcontrollers, and (3) accuracy and precision testing compared with laboratory measurements. The precision of the integrated IoT water sensors was assessed through a dedicated sensor precision test, resulting in an accuracy rate of 94.4% for the turbidity sensor and 97.5% for the TDS sensor. Notably, this approach successfully discriminated drinking water with valid categorization, while other water types, including groundwater, water with tea, and water with coffee, yielded null categorization results.

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Is it Possible to Produce Canned Champignon Mushrooms with Less Sulfite?

SSRN

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SSRN 2023年

作者： Coelho, Vinicius Serafim Modolo, Mariana Uliana de Jesus Filho, Milton Macedo, Leandro Levate Cardoso, Wilton Soares Pinheiro, Flávia de Abreu Federal Institute of Espírito Santo Venda Nova do Imigrante Espírito Santo Brazil Graduate Program in Food Science and Technology Center for Agricultural Sciences and Engineering Federal University of Espírito Santo Espírito Santo Alegre Brazil Department of Pharmacy Federal University of Juiz de Fora Governador Valadares Minas Gerais Governador Valadares Brazil

This study aimed to reduce sulfite levels in canned mushroom (Agaricus bisporus). Formulations were developed: without sulfite and condiment (F1);without sulfite and with condiment (F2);with sulfite and without condiment (F3);with sulfite and condiment (F4). Control (C) consisted of traditional canned mushrooms prepared by the producer. The treatments were evaluated over 42 days. Acidity and pH analysis showed that canned C and F1 maintained equilibrium pH ( © 2023, The Authors. All rights reserved.

关键词： Fungi

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Is Attention All You Need for EEG to Predict Neurological Outcomes in Cardiac Arrest?

Is Attention All You Need for EEG to Predict Neurological Ou...

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IEEE EMBS International Conference on Information technology Applications in Biomedicine (ITAB)

作者： Jefferson Dionisio Muhammad Rizwan Hidetaka Suzuki Kazi Mahmudul Hassan Ingon Chanpornpakdi Lian-Yu Lin Toshihisa Tanaka Che Lin Department of Smart Medicine and Health Informatics National Taiwan University Taipei Taiwan Artificial Intelligence of Things Taiwan International Graduate Program Academia Sinica Taipei Taiwan Tokyo University of Agriculture and Technology Tokyo Japan Department of Emergency and Critical Care Japanese Red Cross Musashino Hospital Tokyo Japan Department of Internal Medicine Division of Cardiology National Taiwan University College of Medicine and Hospital Taipei Taiwan Department of Electrical Engineering National Taiwan University Taipei Taiwan Graduate Institute of Communication Engineering National Taiwan University Taipei Taiwan Center for Advanced Computing and Imaging in Biomedicine National Taiwan University Taipei Taiwan

ISBN: (数字)9798350351552

ISBN: (纸本)9798350351569

Sudden cardiac arrest (SCA) poses a significant health challenge, necessitating accurate predictions of neurological outcomes in comatose patients, where good outcomes are defined as the recovery of most cognitive functions. Electroencephalogram (EEG) serves as a valuable biomarker for monitoring neurological states due to its rich, time-dependent information. This study aims to predict neurological outcomes using early EEG data by employing the Transformer model, which leverages multi-headed attention to identify patterns in lengthy sequences such as hour-long EEG recordings. Unlike traditional methods that use subsampled EEG epochs, we utilize the entire EEG sequences, subdivided into time steps, allowing our model to capture detailed temporal patterns via the attention mechanism. Moreover, we trained our proposed model using each EEG recording as an individual data sample but evaluated our model through aggregated patient-wise predictions. This approach allows us to boost the data sample size. Our results demonstrate promising predictive performance, achieving an AUROC of 0.82 and AUPRC of 0.90 on the holdout test set and an AUROC of 0.73 and AUPRC of 0.93 on an external test set with patient-wise predictions. This study highlights the potential of utilizing attention mechanisms to capture important time series progressions across EEG sequences for improving SCA prognosis.

关键词： Attention mechanisms Biological system modeling Time series analysis Cardiac arrest Predictive models Brain modeling Transformers Electroencephalography Data models Recording

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The biocompatibility and hemostatic efficacy of silk fibroin nanofibrils fabricated by Shear-Induced phase separation process

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Chemical engineering Journal 2023年 474卷

作者： Lu, Po-Wen Wu, Yu-Tzu Chang, Liang-Yu Lu, Tzu-Wei Jaihao, Chonlachat Huang, Ching-Shuan Murphy Chuang, Mo-Fei Lin, Chunche Chen, Innie Brangule, Agnese Zarkov, Aleksej Kareiva, Aivaras Yang, Jen-Chang Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei11052 Taiwan Division of Gastroenterology and Hepatology Department of Internal Medicine Shuang Ho Hospital Taipei Medical University Zhongzheng Rd. Taipei Zhonghe23561 Taiwan Graduate Institute of Nanomedicine and Medical Engineering College of Biomedical Engineering Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Department of Dentistry Taipei Medical University Hospital Taipei11031 Taiwan Institute of Organic and Polymeric Materials National Taipei University of Technology Taipei106344 Taiwan Research and Development Center for Smart Textile Technology National Taipei University of Technology Taipei106344 Taiwan Innie Chen Department of Obstetrics and Gynecology University of Ottawa OttawaONK1H 7W9 Canada Department of Pharmaceutical Chemistry Riga Stradins University RīgaLV-1007 Latvia Institute of Chemistry Vilnius University Naugarduko 24 VilniusLT-03225 Lithuania International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Research Center of Biomedical Device Taipei Medical University Taipei11031 Taiwan Research Center of Digital Oral Science and Technology Taipei Medical University Taipei11031 Taiwan

The objectives of this study were to investigate the biocompatibility and hemostatic efficacy of silk fibroin nanofibrils (SFNFs). Unlike a conventional dialysis/freeze-drying process, SFNFs were prepared using a novel shear-induced phase-separation method. The morphology, particle size distribution, protein secondary structures, and crystalline structures of the resulting SFNFs were respectively investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), laser diffraction (LD), Fourier-transform infrared (FTIR) spectroscopy, and x-ray diffraction (XRD) deconvolution analyses. Polar and non-polar extractants of SFNFs under a bath ratio of 0.2 g/mL were collected after individually soaking them into 0.9% normal saline and cottonseed oil. In vitro hemolysis ratios, biocompatibility, and sensitization tests were carried out. Then, the hemostatic efficacy was assayed using a rat liver punch biopsy model. Compared to dialyzed/freeze-dried SF, the harvested SFNFs revealed high surface area in morphology. The median particle size distribution, D50, was 294 μm for SFNFs. Shear-induced hydrogen bonding, crystallization, and phase separation tended to give the SFNFs a high crystalline index (CI) and degree of crystallinity (DC). The hemolysis ratio of SFNFs was 0.86 ± 0.40%, and it was classified as being non-hemolytic (0%∼2%). The test results of toxicity and skin sensitization were both negative. The hemostasis time for SFNFs (2.8 ± 0.6 min) was statistically less than that of oxidized cellulose (4.3 ± 0.9 min) (p © 2023 Elsevier B.V.

关键词： Biocompatibility

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Enhanced Cuproptosis via Metabolic Reprogramming Using Copper-Delivering Co-N-C Single-Atom Nanozyme

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ACS nano 2025年第24期19卷 2025 Jun 11页

作者： Kang Kim Jaewoo Lee Ok Kyu Park Hyochul Lee Taekyu Jang Jungho Kim Bowon Lee Jeong Hyun Kim Jaeho Moon Seoin Back Nohyun Lee Seung Hong Choi Taeghwan Hyeon Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Republic of Korea. School of Chemical and Biological Engineering and Institute of Chemical Processes Seoul National University Seoul 08826 Republic of Korea. Department of Radiology Seoul National University College of Medicine Seoul 03080 Republic of Korea. Interdisciplinary Program in Cancer Biology Seoul National University College of Medicine Seoul 03080 Republic of Korea. Department of Chemical and Biomolecular Engineering Sogang University Seoul 04107 Republic of Korea. KU-KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Republic of Korea. Department of Integrative Energy Engineering Korea University Seoul 02841 Republic of Korea. School of Advanced Materials Engineering Kookmin University Seoul 02707 Republic of Korea.

Cuproptosis, a copper-dependent cell death, has shown promise as a cancer therapy characterized by mitochondrial protein aggregation. However, this distinct feature makes its efficacy highly dependent on the metabolic state of cancer cells, yielding poor outcomes in hypoxic tumors with a reduced mitochondrial activity. Herein, we construct a Cu-delivering vehicle with catalase-like activity using oxygen-rich cobalt single-atom nanozymes (Cu@CoNC(O)) to reprogram metabolism while delivering copper. By introduction of oxygen-containing functional groups onto carbon supports, Cu@CoNC(O) exhibits significantly improved loading efficiency of Cu ions compared to its oxygen-deficient counterpart. Simultaneously, the outstanding catalase-like activity of Cu@CoNC(O) alleviates hypoxia, switching metabolism from glycolysis to mitochondrial respiration through the inhibition of lactate metabolism and activation of the pyruvate dehydrogenase complex. The synergistic effect of the metabolic shift and efficient Cu delivery promotes cuproptosis even under hypoxic conditions, leading to enhanced therapeutic effects. This study demonstrates the potential of single-atom nanozymes as catalytic ion delivery vehicles capable of concurrently performing catalytic functions and delivering metal ions, presenting an effective strategy to enhance cuproptosis through metabolic modulation.

关键词： catalase-like activity cuproptosis hypoxia relief ion delivery metabolic reprogramming single-atom nanozymes

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Measurement of Water Diffusion Coefficient in Cross-Linked Gelatins Using the Terahertz Time-Domain Spectroscopy Technique

Measurement of Water Diffusion Coefficient in Cross-Linked G...

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Conference on Lasers and Electro-Optics (CLEO)

作者： D. Walden D. Chakraborty J. Cheng A. Straksys A. Stirke W. CMA Melo A. Jukna I. Komissarov Roman Sobolewski Department of Physics and Astronomy University of Rochester Rochester New York USA Laboratory for Laser Energetics University of Rochester Rochester New York USA Materials Science Graduate Program University of Rochester Rochester New York USA State Research Institute Center for Physical Sciences & Technology Vilnius Lithuania Department of Physics Vilnius Gediminas Technical University Vilnius Lithuania Department of Electrical and Computer Engineering University of Rochester Rochester New York USA

A novel method has been developed to evaluate the water diffusion dynamics in gelatin-based gels, based on transient terahertz time-domain spectroscopy measurements. Such gels are widely used models for human skin.

ISBN: (纸本)9798350369311

关键词： Spectroscopy Measurement by laser beam Lasers and electrooptics Laser modes Skin Time-domain analysis Transient analysis Electro-optical waveguides

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Symmetry-driven Phononic Metamaterials

arXiv

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arXiv 2024年

作者： Yves, Simon Fleury, Romain Shmuel, Gal Vitelli, Vincenzo Haberman, Michael R. Alù, Andrea Photonics Initiative Advanced Science Research Center City University of New York New YorkNY10031 United States Laboratory of Wave Engineering EPFL Lausanne1015 Switzerland Faculty of Mechanical Engineering Technion Israel Institute of Technology Haifa32000 Israel University of Chicago James Franck Institute 929 E 57th Street ChicagoIL60637 United States University of Chicago Kadanoff Center for Theoretical Physics 933 E 56th St ChicagoIL60637 United States Walker Department of Mechanical Engineering The University of Texas at Austin AustinTX78712 United States Physics Program Graduate Center City University of New York New YorkNY10026 United States

Phonons are quasi-particles associated with mechanical vibrations in materials, at the root of the propagation of sound, elastic / mechanical waves, and of thermal phenomena, common to our every day life and many technologies. The fundamental understanding and control over phonon responses in natural and artificial media is of major importance in the context of telecommunications, shielding, energy harvesting and control, sensing and imaging, across multiple scales. In this context, it has been recently realized that controlling different classes of symmetries at the microscopic and mesoscopic scale offers a powerful rational tool to precisely tailor phononic responses, leading to advanced acoustic and elastodynamic wave control. In this paper, we review the recent advances in the design and synthesis of artificial phononic media, namely phononic metamaterials, guided by symmetry principles. Starting from tailored broken spatial symmetries, we discuss their interplay with time symmetries for non-reciprocity and non-conservative phenomena, and finally address broader concepts that combine multiple symmetry classes to support exotic phononic wave transport. © 2024, CC BY.

关键词： Phonons

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Electrospun Nanofibers from Poly (Ε-Caprolactone) with Copaiba (Copaifera Sp)Oleoresin as an Advanced Healer

SSRN

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SSRN 2024年

作者： Marcos Pinho da Silva, Andrey Miotto, Marília Afonso Provenzi, Marcel Lopes, Susane Maraschin, Marcelo Merlini, Claudia Sciences and Materials Engineering Graduate Program Federal University of Santa Catarina Reitor João David Ferreira Lima University Campus SC Florianópolis88040-900 Brazil Department of Materials Engineering Technology College Federal University of Amazonas AM Manaus Brazil Food Microbiology Laboratory Departament of Food Science and Technology Federal University of Santa Catarina Agrarian Sciences Center Av. Admar Gonzaga 1346 - Itacorubi SC . Florianópolis88034-001 Brazil Florianópolis Brazil Rua João Pessoa 2750 Bairro Velha Blumenau SC 89036-256 Brazil

Advanced healing developed using electrospinning show advantages such as highsurface area/volume relation, similarity with the skin extracellular matrix and ability toincorporate bioactive agents. The use of natural compounds, as the copaiba oleoresinresults in benefits due to its action as enhancer of biochemical agents during theinflammatory phase in the healing process. This study investigated the development ofnanofiber from polycaprolactone and copaiba oleoresin aiming at demonstrating itsantimicrobial and atoxic properties with healing effect on complex wounds. Thecopaiba oleoresin chemical composition revealed the predominance of sesquiterpenes,with majoritary components such as Caryophyllane, γ-Muurolene, and α-Copaene. Thenanofibers were anlyzed employing several techniques and revealed suitablephysicochemical characteristics for the development of advanced healing. The zetapotential indicated a negative surface load in the nanofibers, suggesting favorableinteractions for biomedical applications such as cell adherence. The copaiba oleoresinpresence significantly increased the liquid absorption ability, fundamental in advancedhealing, and accelerated the mass loss rate, emphasizing its influence in thebiodegradability of the nanofibers. The nanofibers showed efficacy againstStaphylococcus Aureus, while the cell viability assay indicared absence of *** results suggest the potential of these nanofibers as efficient and sustainableantimicrobial healing for clinical applications © 2024, The Authors. All rights reserved.

关键词： Nanofibers

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