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label-Free sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device

MICROMACHINES 2023年第2期14卷 407-407页

作者： Yang, Bowen Wang, Chao Liang, Xinyi Li, Jinchao Li, Shanshan Wu, Jie Jayne Su, Tanbin Li, Junwei Hebei Univ Technol Sch Mech Engn Hebei Key Lab Smart Sensing & Human Robot Interact Tianjin 300130 Peoples R China Hebei Univ Technol Inst Biophys Sch Hlth Sci & Biomed Engn Tianjin 300401 Peoples R China Hebei Univ Technol State Key Lab Reliabil & Intelligence Elect Equipm Tianjin 300132 Peoples R China Univ Tennessee Dept Elect Engn & Comp Sci Knoxville TN 37919 USA

Cell viability is an essential physiological status for drug screening. While cell staining is a conventional cell viability analysis method, dye staining is usually cytotoxic. Alternatively, impedance cytometry provides a straightforward and label-free sensing approach for the assessment of cell viability. A key element of impedance cytometry is its sensing electrodes. Most state-of-the-art electrodes are made of expensive metals, microfabricated by lithography, with a typical size of ten microns. In this work, we proposed a low-cost microfluidic impedance cytometry device with 100-micron wide indium tin oxide (ITO) electrodes to achieve a comparable performance to the 10-micron wide Au electrodes. The effectiveness was experimentally verified as 7 mu m beads can be distinguished from 10 mu m beads. To the best of our knowledge, this is the lowest geometry ratio of the target to the sensing unit in the impedance cytometry technology. Furthermore, a cell viability test was performed on MCF-7 cells. The proposed double differential impedance cytometry device has successfully differentiated the living and dead MCF-7 cells with a throughput of similar to 1000 cells/s. The label-free and low-cost, high-throughput impedance cytometry could benefit drug screening, fundamental biological research and other biomedical applications.

关键词： microfluidic impedance cytometry low-cost ITO electrodes cell viability analysis label-free sensing sensitive electrodes

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Cellulose nanofiber enabled high-mass loading N-doped porous carbon electrode for zinc-ion supercapacitors

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SUSTAINABLE MATERIALS AND TECHNOLOGIES 2024年 41卷

作者： Zhao, Zhen Hu, Yong-Peng Liu, Kai-Yang Yu, Wei Li, Guo-Xian Meng, Chui-Zhou Guo, Shi-Jie Hebei Univ Technol Hebei Key Lab Smart Sensing & Human Robot Interact 5340 Xiping Rd Tianjin 300401 Peoples R China Hebei Univ Technol Engn Res Ctr Minist Educ Intelligent Rehabil Equipment & Detect 5340 Xiping Rd Tianjin 300401 Peoples R China Hebei Univ Technol Sch Mech Engn 5340 Xiping Rd Tianjin 300401 Peoples R China

Zinc-ion hybrid supercapacitors (ZHSCs) are among the most promising electrochemical energy storage technologies because of their high energy density, environmental sustainability, low price, and inherent security. High-load, high-capacity cathodes are critical for the practical application of ZHSCs. However, it is challenging to achieve optimal performance of high-loading electrodes due to slow reaction kinetics, retarded ion transport, and structural instability. Herein, a salt-template technique was used to fabricate nitrogen-doped porous carbon (NPC) with large surface area, abundant mesopores, and electroactive nitrogen dopants. When combined with a fibrous network scaffold of carbon nanotubes and cellulose nanofibers (CNT/CNF), the optimized NPC delivered an outstanding specific capacitance of 133.4 mAh g-1 and a remarkable capacity retention of 95.4% after 10,000 cycles. More impressively, we achieved a cathode with high mass-loading of 10 mg cm-2 without significantly sacrificing the electrochemical performance (area capacity of 2.05 mAh cm-2 and good cycling stability with capacity retention of 86.2% after 3000 cycles). The excellent performance can be attributed to the continuous electron/ion conduction pathways of CNT/CNF scaffold and redox-active surface generated by the favorable heteroatom-doped porous carbon. In sum, this work offers a platform for making high mass-loading carbon electrodes for practical ZHSCs.

关键词： Zinc -ion hybrid supercapacitors Cellulose nanofiber Porous carbon Nitrogen doping

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Virtual Multiplexing Chamber-Based Digital PCR for Camel Milk Authentication Applications

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MICROMACHINES 2023年第8期14卷 1619-1619页

作者： Li, Jinchao Cheng, Jingmeng Li, Shanshan Wu, Jie Jayne Li, Junwei Hebei Univ Technol Sch Mech Engn Hebei Key Lab Smart Sensing & Human Robot Interact Tianjin 300130 Peoples R China Hebei Univ Technol State Key Lab Reliabil & Intelligence Elect Equip Tianjin 300130 Peoples R China Univ Tennessee Dept Elect Engn & Comp Sci Knoxville TN 37996 USA Hebei Univ Technol Inst Biophys Sch Hlth Sci & Biomed Engn Tianjin 300401 Peoples R China

In this work, we proposed a chamber-based digital PCR (cdPCR) microfluidic device that is compatible with fluorescence imaging systems for milk adulteration detection. The device enables the digitalization of PCR reagents, which are loaded into microchambers, and subsequent thermocycling for DNA amplification. Then, fluorescence images of the microchambers are captured and analyzed to obtain the total number of positive chambers, which is used to calculate the copy numbers of the target DNA, enabling accurate quantitative detections to determine intentional milk adulteration from accidental contaminations. The validation of this device is performed by camel milk authentication. We performed 25,600-chamber virtual multiplexing cdPCR tests using 40 x 40 chamber devices for the detection of DNA templates extracted from pure or mixed milk with different dilutions. Then, the cdPCR chip was used to authenticate blind milk samples, demonstrating its efficacy in real biotechnical applications.

关键词： microfluidics digital polymerase chain reaction camel authentication

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Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications

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MICROMACHINES 2021年第6期12卷 694-694页

作者： Hu, Hezhi Cheng, Jingmeng Wei, Chunyang Li, Shanshan Yu, Chengzhuang Meng, Xiaoshuai Li, Junwei Hebei Univ Technol State Key Lab Reliabil & Intelligence Elect Equip Tianjin 300132 Peoples R China Hebei Univ Technol Dept Elect & Informat Engn Langfang 065000 Peoples R China Sch Mech Engn Hebei Key Lab Smart Sensing & Human Robot Interac Tianjin 300130 Peoples R China Jiangsu Key Lab Adv Food Mfg Equipment & Technol Wuxi 214122 Jiangsu Peoples R China Hebei Univ Technol Sch Sci Inst Biophys Tianjin 300401 Peoples R China

Droplet digital polymerase chain reaction (ddPCR) suffers from the need for specific equipment and skilled personnel;thus, we here present a chamber-based digital PCR microfluidic device that is compatible with fluorescence image read-out systems and removes bubbles by a pre-degassed microfluidic device that consists of a pilot channel and micro chamber arrays. Digitalized PCR reagents are introduced into micro chambers, and thermocycles are taken to perform a DNA amplification process. Then, fluorescence images of a micro chamber array are read out and analyzed to obtain the total number of positive chambers. Thereby, the copy numbers of target DNA are calculated for quantitative detections. As a validation, this device is evaluated by the application of meat authentication. We performed dPCR tests using DNA templates extracted from a pure mutton DNA template with different dilutions. Then, the dPCR chip was used to identify the meat authentication using mutton-chicken mixtures with different mass ratios, showing its performance in real biotechnical applications.

关键词： microfluidics digital polymerase chain reaction degas meat authentication

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Easy-to-operate fabrication of tapered glass capillaries for microdroplet generation

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JOURNAL OF MICROMECHANICS AND MICROENGINEERING 2019年第3期29卷 037001-037001页

作者： Wei, Chunyang Yu, Chengzhuang Wu, Jie Jayne Li, Junwei Li, Shanshan Dai, Shijie Li, Tiejun Hebei Univ Technol Sch Mech Engn Tianjin 300401 Peoples R China Hebei Key Lab Smart Sensing & Human Robot Interac Tianjin 300130 Peoples R China Univ Tennessee Dept Elect Engn & Comp Sci Knoxville TN 37996 USA Hebei Univ Technol Dept Comp Sci & Elect Engn Langfang 000650 Peoples R China State Key Lab Reliabil & Intelligence Elect Equip Tianjin 300132 Peoples R China

Tapered glass capillaries play an important role in microdroplet generation and related microfluidic applications such as particle synthesis, digital PCR and microrectors. However, its controlled production poses a challenge. Specifically, there is difficulty controlling the tip sizes of capillaries with good repeatability at an affordable cost, which is critical to produce standard droplets for use by research groups. To produce tapered capillaries, micro needle geometries are first pulled by a commercial pipette puller, which yields a sharp tip (i.e. 1-3 mu m). Those tips need to be enlarged and standardized by post microfabrication. Although larger tips could be achieved by advanced pulling-forging, sanding-forging or breakingforging methods, the uniformity and repeatability do not meet the requirements for mass production. In this paper, several easy-to-operate fabrication approaches, including mechanical and chemical fabrication methods, are studied. Chemical etching fabrication method shows good repeatability and robust control of dimensions. A capillary droplet microfluidic device assembled by tapered glass capillaries fabricated by chemical etching method is also presented, which shows good performance for droplet generations. This non-labor intensive fabrication is straightforward and be readily adopted for industrial scale mass production.

关键词： micro fabrication glass capillary taper droplet generation

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Study on Effect of Surface Micro-Texture of Cemented Carbide on Tribological Properties of Bovine Cortical Bone

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MICROMACHINES 2024年第8期15卷 994-994页

作者： Shang, Peng Liu, Bingfeng Guo, Chunhai Cui, Peijuan Hou, Zhanlin Jin, Fengbin Zhang, Jianjun Guo, Shijie Huang, Yuping Zhang, Wenwu Hebei Univ Technol Sch Mech Engn Hebei Key Lab Smart Sensing & Human Robot Interact Minist Educ Intelligent Rehabil Device & Detect Te Tianjin 300401 Peoples R China Chinese Acad Sci Ningbo Inst Mat Technol & Engn Ningbo 315201 Peoples R China Beijing Inst Precis Mechatron & Controls Beijing 100076 Peoples R China

In bone-milling surgical procedures, the intense friction between the tool and bone material often results in high cutting temperatures, leading to the thermal necrosis of bone cells. This paper aims to investigate the effect of micro-texture on the tribological properties of YG8 cemented carbide in contact with bone. The main objective is to guide the design of tool surface microstructures to reduce frictional heat generation. To minimize experimental consumables and save time, numerical simulations are first conducted to determine the optimal machining depth for the texture. Subsequently, micro-textures with different shapes and pitches are prepared on the surface of YG8 cemented carbide. These textured samples are paired with bovine cortical bone pins featuring various bone unit arrangements, and friction and wear tests are conducted under physiological saline lubrication. The experimental results indicate that the appropriate shape and pitch of the micro-texture can minimize the coefficient of friction. The parallel arrangement of bone units exhibits a lower coefficient of friction compared to the vertical arrangement. This study holds significant implications for the design and fabrication of future micro-texture milling cutters.

关键词： micro-texture fluid simulation cemented carbide bovine cortical bone friction coefficient

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Antidisturbance Control for Hidden Markovian Jump Systems: Asynchronous Disturbance Observer Approach

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IEEE TRANSACTIONS ON AUTOMATIC CONTROL 2023年第11期68卷 6982-6989页

作者： Song, Jun Zhou, Sai Niu, Yugang Cao, Zhiru He, Shuping Anhui Univ Minist Educ Engn Res Ctr Autonomous Unmanned Syst Technol Hefei 230601 Peoples R China Anhui Univ Sch Artifcial Intelligence Anhui Prov Engn Res Ctr Unmanned Syst & Intelligen Hefei 230601 Peoples R China East China Univ Sci & Technol Minist Educ Key Lab Smart Mfg Energy Chem Proc Shanghai 200237 Peoples R China Shanghai Univ Sch Mechatron Engn & Automat Shanghai 200072 Peoples R China Anhui Univ Informat Mat & Intelligent Sensing Lab Anhui Prov Hefei 230601 Peoples R China Anhui Univ Sch Elect Engn & Automat Anhui Engn Lab Human Robot Integrat Syst & Intelli Hefei 230601 Peoples R China

This article investigates the antidisturbance control issue for a class of Markovian jump systems with matched/mismatched disturbances by using disturbance observer approach. A key feature here is that the external disturbance is considered to be generated by a Markovian jump exogenous dynamics, in which the disturbance mode may not be synchronized with the system mode. Meanwhile, it is supposed that both system and disturbance modes can just be estimated via a hidden Markov mode detector. To overcome the possible obstacle in handling dual hidden Markov modes, a mapping technique for continuous-time Markov chain is provided first. Then, a novel asynchronous disturbance observer is developed to estimate the external disturbance by utilizing the detected mode. Based upon the estimated disturbance, asynchronous antidisturbance controllers are investigated for matched and mismatched disturbance cases, respectively. By employing a decoupling technique with the idea of completing the square, sufficient conditions are established for guaranteeing the stability in probability of the resultant closed-loop systems. Finally, two numerical examples are provided to verify the effectiveness of the proposed asynchronous antidisturbance control approaches under inaccessible system and disturbance modes.

关键词： Antidisturbance control disturbance observer hidden Markov detector Markovian jump systems (MJSs)

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Self-Healing, Reconfigurable, Thermal-Switching, Transformative Electronics for Health Monitoring

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ADVANCED MATERIALS 2023年第15期35卷 2207742-2207742页

作者： Yang, Li Wang, Zihan Wang, Hao Jin, Biqiang Meng, Chuizhou Chen, Xue Li, Runze Wang, He Xin, Mingyang Zhao, Zeshang Guo, Shijie Wu, Jinrong Cheng, Huanyu Hebei Univ Technol Sch Hlth Sci & Biomed Engn State Key Lab Reliabil & Intelligence Elect Equipm Tianjin 300130 Peoples R China Hebei Univ Technol Sch Mech Engn State Key Lab Reliabil & Intelligence Elect Equipm Hebei Key Lab Smart Sensing & Human Robot Interact Tianjin 300401 Peoples R China Sichuan Univ Coll Polymer Sci & Engn State Key Lab Polymer Mat Engn Chengdu 610065 Peoples R China Hebei Univ Technol Sch Elect Engn State Key Lab Reliabil & Intelligence Elect Equipm Key Lab Bioelectromagnet & Neuroengn Hebei Prov Tianjin 300130 Peoples R China Penn State Univ Dept Engn Sci & Mech University Pk PA 16802 USA

Soft, deformable electronic devices provide the means to monitor physiological information and health conditions for disease diagnostics. However, their practical utility is limited due to the lack of intrinsical thermal switching for mechanically transformative adaptability and self-healing capability against mechanical damages. Here, the design concepts, materials and physics, manufacturing approaches, and application opportunities of self-healing, reconfigurable, thermal-switching device platforms based on hyperbranched polymers and biphasic liquid metal are reported. The former provides excellent self-healing performance and unique tunable stiffness and adhesion regulated by temperature for the on-skin switch, whereas the latter results in liquid metal circuits with extreme stretchability (>900%) and high conductivity (3.40 x 10(4) S cm(-1)), as well as simple recycling capability. Triggered by the increased temperature from the skin surface, a multifunctional device platform can conveniently conform and strongly adhere to the hierarchically textured skin surface for non-invasive, continuous, comfortable health monitoring. Additionally, the self-healing and adhesive characteristics allow multiple multifunctional circuit components to assemble and completely wrap on 3D curvilinear surfaces. Together, the design, manufacturing, and proof-of-concept demonstration of the self-healing, transformative, and self-assembled electronics open up new opportunities for robust soft deformable devices, smart robotics, prosthetics, and Internet-of-Things, and human-machine interfaces on irregular surfaces.

关键词： liquid metal printed circuits multifunctional electronic devices self-healing films thermal-switching three-dimensional conformal self-assembly transformative characteristics

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Stretchable Triboelectric Nanogenerator Based on Liquid Metal with Varying Phases

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ADVANCED SCIENCE 2024年第39期11卷 e2405792页

作者： Yang, Li Guo, Langang Wang, Zihan Meng, Chuizhou Wu, Jinrong Chen, Xue Abu Musa, Abdullah Jiang, Xiaoqi Cheng, Huanyu Hebei Univ Technol Sch Hlth Sci & Biomed Engn State Key Lab Reliabil & Intelligence Elect Equip Tianjin 300130 Peoples R China Hebei Univ Technol Sch Mech Engn State Key Lab Reliabil & Intelligence Elect Equip Hebei Key Lab Smart Sensing & Human Robot Interac Tianjin 300401 Peoples R China Sichuan Univ Coll Polymer Sci & Engn State Key Lab Polymer Mat Engn Chengdu 610065 Peoples R China Hebei Univ Technol Sch Elect Engn State Key Lab Reliabil & Intelligence Elect Equip Key Lab Bioelectromagnet & Neuroengn Hebei Prov Tianjin 300130 Peoples R China Penn State Univ Dept Engn Sci & Mech University Pk PA 16802 USA

Stretchable triboelectric nanogenerators (TENGs) represent a new class of energy-harvesting devices for powering wearable devices. However, most of them are associated with poor stretchability, low stability, and limited substrate material choices. This work presents the design and demonstration of highly stretchable and stable TENGs based on liquid metalel ectrodes with different phases. The conductive and fluidic properties of eutectic gallium-indium (EGaIn) in the serpentine microfluidic channel ensure the robust performance of the EGaIn-based TENG upon stretching over several hundred percent. The bi-phasic EGaIn (bGaIn) from oxidation lowers surface tension and increases adhesion for printing on diverse substrates with high output performance parameters. The optimization of the electrode shapes in the bGaIn-based TENGs can reduce the device footprint and weight, while enhancing stretchability. The applications of the EGaIn- and bGaIn-based TENG include smart elastic bands for human movement monitoring and smart carpets with integrated data transmission/processing modules for headcount monitoring/control. Combining the concept of origami in the paper-based bGaIn TENG can reduce the device footprint to improve output performance per unit area. The integration of bGaIn-TENG on a self-healing polymer substrate with corrosion resistance against acidic and alkaline solutions further facilitates its use in various challenging and extreme environments. This work presents stretchable and stable triboelectric nanogenerators (TENGs) based on liquid metal with different crystalline phases. The eutectic gallium-indium(EGaIn)-based TENG can be stretched over 800%, whereas the bi-phasic-EGaIn (bGaIn) lowers surface tension and increases adhesion for printing on diverse substrates. The optimization of electrode shapes in the bGaIn-based TENGs reduces the device footprint and weight while providing stretchable properties. image

关键词： liquid metal multi-substrate electrode stretchable triboelectric nanogenerators

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Nucleic Acid Detection with Ion Concentration Polarization Microfluidic Chip for Reduced Cycle Numbers of Polymerase Chain Reaction

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MICROMACHINES 2022年第9期13卷 1394-1394页

作者： Yu, Chengzhuang Dai, Shijie Li, Shanshan Li, Junwei Hu, Hezhi Meng, Jiyu Wei, Chunyang Wu, Jie Jayne Hebei Univ Technol Sch Mech Engn Hebei Key Lab Smart Sensing & Human Robot Interac Tianjin 300130 Peoples R China Hebei Univ Technol Sch Elect Engn State Key Lab Reliabil & Intelligence Elect Equip Tianjin 300130 Peoples R China Hebei Univ Technol Sch Hlth Sci & Biomed Engn Inst Biophys Tianjin 300130 Peoples R China Hebei Univ Technol Dept Elect & Informat Engn Langfang 065099 Peoples R China Univ Tennessee Dept Elect Engn & Comp Sci Knoxville TN 37996 USA

Nucleic acid detection is widely used in disease diagnosis, food safety, environmental monitoring and many other research fields. The continuous development of rapid and sensitive new methods to detective nucleic acid is very important for practical application. In this study, we developed a rapid nucleic-acid detection method using polymerase chain reaction (PCR) combined with electrokinetic preconcentration based on ion concentration polarization (ICP). Using a Nafion film, the proposed ICP microfluidic chip is utilized to enrich the nucleic acid molecules amplified by PCR thermal cycles. To demonstrate the capability of the microfluidic device and the hybrid nucleic-acid detection method, we present an animal-derived component detection experiment for meat product identification applications. With the reduced cycle numbers of 24 cycles, the detection can be completed in about 35 min. The experimental results show that this work can provide a microfluidic device and straightforward method for rapid detection of nucleic acids with reduced cycle numbers.

关键词： microfluidic nucleic acid detection polymerase chain reaction electrokinetic preconcentration ion concentration polarization

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