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A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling

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Chinese Chemical Letters 2022年第6期33卷 3091-3096页

作者： Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China The Key Laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China

The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular *** reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical *** this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination *** on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable ***,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation *** maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple *** show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La ***,statistical analysis was performed for the mean peak height and mean peak area of the cellular *** believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications.

关键词： Microfluidics Dynamic stimulation Time code High-throughput Single-cell array

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Building Supporting SLAM Community for IEEE TIV: From DHWs to Smart Academic Organizations

IEEE Transactions on Intelligent Vehicles

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IEEE Transactions on intelligent Vehicles 2024年第7期9卷 5119-5123页

作者： Fei-Yue Wang Wuhan Institute of Industrial Innovation and Development Hubei China State Key Laboratory for Management and Control of Complex Systems Institute of Automation Chinese Academy of Sciences Beijing China

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关键词： Simultaneous localization and mapping intelligent vehicles Buildings Organizations

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From RAG/RAT to SAGE for Social Transportation: A CoT and New Perspective on Smart Logistics and Mobility

IEEE Transactions on Intelligent Vehicles

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IEEE Transactions on intelligent Vehicles 2024年第6期9卷 5005-5008页

作者： Fei-Yue Wang Wuhan Institute of Industrial Innovation and Development Hubei China The State Key Laboratory for Management and Control of Complex Systems Institute of Automation Chinese Academy of Sciences Beijing China

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关键词： intelligent vehicles Transportation Logistics

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The Autonomous Right of Way: Smart Governance for Smart Mobility With intelligent Vehicles

IEEE Transactions on Intelligent Vehicles

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IEEE Transactions on intelligent Vehicles 2024年第8期9卷 5243-5246页

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Research on Urban Air Quality Prediction Method Based on the Fusion of Wide-area Spatiotemporal Distribution Data

Research on Urban Air Quality Prediction Method Based on the...

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IEEE International Conference on Industrial Technology (ICIT)

作者： Zixing Tao Weihua Cao Chao Gan School of Future Technology China University of Geosciences (Wuhan) Wuhan China School of Future Technology School of Automation Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems Engineering Research Center of Intelligent Technology for Geo-Exploration Ministry of Education China University of Geosciences (Wuhan) Wuhan China

ISBN: (数字)9798331521950

ISBN: (纸本)9798331521967

Air quality data exhibit nonlinearity, sensitivity to environmental factors, and long-term dependencies. Numerous factors influence air quality, making accurate predictions based on a single-dimensional dataset impractical. This study proposes a method for urban air quality prediction that integrates wide-area spatiotemporal data, designed to address the unique characteristics of air quality datasets. First, relevant wide-area spatiotemporal data are selected, and their correlations with air quality are systematically analyzed. Second, a Long Short-Term Memory (LSTM) network-based Transformer model is utilized to capture the long-term dependencies in the air quality sequences. Finally, the model successfully generates hourly multi-step predictions for six air pollutants. The experimental results show that the proposed method outperforms the method that relies on air quality data alone for multi-step air quality prediction.

关键词： Deep learning Sensitivity Atmospheric modeling Time series analysis Predictive models Air quality Transformers Environmental factors Spatiotemporal phenomena Long short term memory

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Real-time In-situ Defect Detection in 3D Printing of Continuous Fiber-Reinforced Composites 4

Real-time In-situ Defect Detection in 3D Printing of Continu...

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4th IEEE International Conference on Digital Twins and Parallel Intelligence, DTPI 2024

作者： Yue, Zhijian Shen, Zhen Dong, Xiangyang Fang, Qihang Wang, Weixing Dong, Xisong Xiong, Gang Wang, Fei-Yue University of Chinese Academy of Sciences School of Artificial Intelligence Beijing100049 China Chinese Academy of Sciences State Key Laboratory of Multimodal Artificial Intelligence Systems Beijing Engineering Research Center of Intelligent Systems and Technology Institute of Automation Beijing100190 China Qingdao Academy of Intelligent Industries Intelligent Manufacturing Center Qingdao266109 China Chinese Academy of Sciences Guangdong Engineering Research Center of 3D Printing and Intelligent Manufacturing Cloud Computing Center Dongguan523808 China Chinese Academy of Sciences State Key Laboratory for Management and Control for Complex Systems Institute of Automation Beijing100190 China

ISBN: (纸本)9798350349252

With the development of continuous fiber-reinforced composites (CFRCs) 3D printing technology, timely, efficient and accurate detection of fiber path defects is essential for ensuring product quality and performance. However, challenges still need to be addressed in this field, such as inadequate training data coverage and the complexity of fiber path structures. These factors hinder the capability of traditional methods to accurately assess the status of printed products. Therefore, developing accurate and robust fiber path defect detection techniques is crucial for CFRCs 3D printing technology. In this paper, we propose a precise detection method for fiber path defects in CFRCs 3D printing using the YOLOv7 object detection model combined with a Squeeze-and-Excitation (SE) attention mechanism. By introducing the SE attention mechanism, we enhance the perception capability of the YOLOv7 model for crucial features, thereby improving the accuracy of fiber path defect detection. The experimental results show that the YOLOv7 model, when augmented with an attention mechanism, achieves an average accuracy of 93.9% in identifying fiber path defects during the printing process. Compared to models without this attention mechanism, the results show that there is 10.7% increase, with a notably enhanced learning capacity during the training phase, which substantiates the efficacy and practicality of the proposed approach. © 2024 IEEE.

关键词： Fiber reinforced plastics

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Multi-Modal Based Fuzzy Atmosfield in Human-Robot Interaction

Multi-Modal Based Fuzzy Atmosfield in Human-Robot Interactio...

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Chinese control Conference (CCC)

作者： Xin-Heng Li Zhen-Tao Liu School of Automation China University of Geosciences (Wuhan) Wuhan China Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems Wuhan China Engineering Research Center of Intelligent Technology for Geo-Exploration Ministry of Education Wuhan China

Recently, the emotional robot has basic functions of perceiving and expressing emotions, but it still hard to communicate naturally between humans and robots. One major reason is that communication atmosphere is seldom considered in Human-robot Interaction (HRI). We propose Multi-modal (i.e., music background, speech, and semantics) Based Fuzzy Atmosfield (FA), which can not only realize the dynamic adjustment of FA but also dynamically regulate human emotions. In the experiment, a Pepper robot is used and one hundred volunteers are invited for HRI, and soothing piano pieces are used as background music. Questionnaires were filled by the volunteers after the experiments, from which the results show that 90% of the volunteers felt the dynamic changes in the communication atmosphere and 77% of the volunteers felt significant emotional regulation, which demonstrates the effectiveness of our method.

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Prediction Method of Truck Travel Time in Open Pit Mines Based on LSTM Model

Prediction Method of Truck Travel Time in Open Pit Mines Bas...

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第42届中国控制会议

作者： Mengting Ao Changhe Li Shengxiang Yang School of Automation China University of Geosciences Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems Engineering Research Center of Intelligent Technology for Geo-Exploration Ministry of Education School of Computer Science and Informatics De Montfort University

Aiming at the prediction of truck travel time in open pit mines,we established a prediction model based on long short-term memory(LSTM).This model fully accounts for 11 factors,including the nature of trucks,weather,road conditions,and driver's behaviors,as well as the influence of neighbor road segments in the route on the current predicted road *** experiment shows that the error of the LSTM prediction model is significantly reduced compared with S VR and BP *** addition,the maximum absolute mean error under different conditions is less than 12 seconds.

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Multi view 3D reconstruction method for weak texture objects based on "eye-in-hand" model

Multi view 3D reconstruction method for weak texture objects...

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2021 China automation Congress, CAC 2021

作者： Gong, Xuan Chen, Xin Jian, Xu He, Wenpeng School of Automation China University of Geosciences Wuhan430074 China Hubei Key Laboratory of Advanced Control Intelligent Automation for Complex Systems China Engineering Research Center of Intelligent Technology for Geo-Exploration Ministry of Education China

ISBN: (纸本)9781665426473

Size measurement and defect detection of ceramics are required in the production process of ceramic products. Therefore, it is necessary to perform high-precision and fast three-dimensional reconstruction of such targets with smooth surfaces and weak textures. However, the reconstruction of a large number of point clouds from multiple perspectives is time-consuming due to the matching of adjacent feature points. Based on this, a fast point cloud registration strategy based on the "eye-in-hand" model is proposed, which can obtain RGB-D images at multi-view around weak-textured objects with RGB-D camera for rapid point cloud registration and reconstruction. Meanwhile, a 3D point cloud segmentation method based on voxel-grid and euclidean distance clustering is applied to divide the scene point and obtain an accurate 3D model. The experimental results indicate that the multi view 3D reconstruction speed of this method is faster and the accuracy of objects point cloud is within 2 mm. Hence, this method can be well applied in reconstructing the 3D point clouds model for ceramic products. © 2021 IEEE

关键词： Textures

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Adaptive Sliding Mode control for Flexible Dual-manipulator with Friction Compensation

Adaptive Sliding Mode Control for Flexible Dual-manipulator ...

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Chinese automation Congress (CAC)

作者： Tao Song Shiqi Zheng Jiachun Sun Liangguang Li Qing Chang Zhipeng Li School of Automation China University of Geosciences Wuhan Campus Hubei Key Laboratory of Advanced Control and Intelligent Automation fo Complex Systems Wuhan China

This paper focuses on the coordinated tracking control scheme of dual-manipulator based on friction compensation. First, a new dual-manipulator model with flexible joints and friction is constructed; Second, a new adaptive neural control method is proposed for the dual-manipulators with flexible joints. Neural networks are used to approximate the unknown nonlinear dynamics in the model; Third, new state observers are constructed to estimate the joint friction. Based on the observers, the friction can be well compensated. The stability of the system is derived by using Lyapunov method. The simulation results verify the effectiveness of the practical method.

关键词： Adaptation models Uncertainty Torque Friction Simulation Neural networks Observers

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