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Vanadium(Ⅳ)solvent extraction enhancement in high acidity using di-(2-ethylhexyl)phosphoric acid with[Cl^(-)]present:an experimental and theoretical study

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Frontiers of Chemical Science and Engineering 2023年第1期17卷 56-67页

作者： Hong Liu Yi-Min Zhang Jing Huang Tao Liu School of Resource and Environmental Engineering Wuhan University of Science and TechnologyWuhan 430081China State Environmental Protection Key Laboratory of Mineral Metallurgical Resources Utilization and Pollution Control Wuhan University of Science and TechnologyWuhan 430081China Hubei Collaborative Innovation Center for High Efficient Utilization of Vanadium Resources Wuhan University of Science and TechnologyWuhan 430081China Hubei Provincial Engineering Technology Research Center of High Efficient Cleaning Utilization for Shale Vanadium Resource Wuhan University of Science and TechnologyWuhan 430081China

Separation of vanadium from black shale leaching solution at low pH is very meaningful,which can effectively avoid the generation of alkali neutralization slag and the resulting vanadium *** this study,coordination mechanism of vanadium in acid leaching solution at low pH was investigated with the intervention of chloride *** the conditions of pH 0.8,di-(2-ethylhexyl)phosphoric acid concentration of 20%,phase ratio of 1:2,and extraction time of 8 min,the vanadium extraction could reach 80.00%.The Fourier transform infrared and electrospray ionization results reveal that,despite the fact that the chloride ion in the leachate could significantly promote vanadium extraction,the chloride ion does not enter the organic phase,indicating an intriguing *** Cl^(-)-V,SO_(4)^(2-)-V,and H_(2)O-V,the V-Cl bond is longer and the potential difference between coordinate ions and vanadium is ***,Vo^(2+)gets easily desorbed with chloride ions and enter the organic *** the same time,the hydrogen ions of di-(2-ethylhexyl)phosphoric acid also enter the water phase more easily,which reduces the pH required for the extraction reaction.

关键词： vanadium black shale solvent extraction high acidity extraction

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Accumulation Patterns of Flavonoids and Phenolic Acids in Different Colored Sweet Potato Flesh Revealed Based on Untargeted Metabolomics

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SSRN 2024年

作者： Wan, Xiaolin Wu, Jiaqi Wang, Xiuzhi Cui, Lingjun Xiao, Qiang Hubei Key Laboratory of Biological Resources Protection and Utilization Hubei Minzu University Enshi44500 China

Currently, limited information is known about the composition of secondary metabolites in different parts of sweet potato. In this investigation, we conducted a comprehensive analysis of the secondary metabolite profiles in the flesh of sweet potatoes exhibiting different colors. Metabolomic profiles of BS, CS and ZS sweetpotato pulp were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 4447 secondary metabolites, including 1540, 1949 and 1931 differentially accumulated metabolites, were identified in the CS vs BS, ZS vs BS and ZS vs CS comparisons. Among them, there were significant differences in flavonoids and phenolic acids. There were 20 flavonoids and 13 phenolic acids that were common differential metabolites among the three comparison groups. And the accumulation of paeoniflorin-like and delphinidin-like compounds may be responsible for the purple coloration of sweet potato pulp. These findings provide new rationale and insights for the development of functional foods for sweet potato. © 2024, The Authors. All rights reserved.

关键词： Metabolites

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Comparative Study of Polysaccharide Metabolites in Purple, Orange, and White Ipomoea Batatas Tubers

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SSRN 2024年

作者： Wang, Xiuzhi Wan, Xiaolin Cui, Lingjun Wu, Jiaqi Xiao, Qiang Hubei Key Laboratory of Biological Resources Protection and Utilization Hubei Minzu University Enshi44500 China

This study aimed to investigate the variations in polysaccharide metabolites and their associated metabolic pathways among Ipomoea batatas tubers of different hues. We utilized an untargeted metabolomics methodology to examine purple, orange, and white types. The study utilized multivariate statistical analyses, specifically PCA and OPLS-DA, along with metabolic pathway analysis, to examine the alterations in metabolic products linked to tuber color. Our study found differences in 23, 36, and 44 polysaccharide metabolites between white-orange, purple-orange, and purple-white tubers, respectively. The OPLS-DA analysis revealed distinct groupings of samples based on their respective tuber colors. The analysis of metabolic pathways primarily identifies and enhances the differential polysaccharide metabolites in various pathways, including Glactose metabolism, among others. These findings expand our comprehension of how color affects the metabolic composition of Ipomoea batatas tubers, which could help enhance crop quality and develop nutritionally varied types. © 2024, The Authors. All rights reserved.

关键词： Metabolites

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Identification of propranolol and derivatives that are chemical inhibitors of phosphatidate phosphatase as potential broad-spectrum fungicides

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Plant Communications 2024年第1期5卷 215-230页

作者： Juan Zhao Yu Chen Zhifen Ding Yaru Zhou Ruiqing Bi Ziting Qin Lei Yang Peng Sun Qiping Sun Guang Chen Daiyuan Sun Xianya Jiang Lu Zheng Xiao-Lin Chen Hu Wan Guanghui Wang Qiang Li Huailong Teng Guotian Li National Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Laboratory of Plant PathologyThe Center of Crop NanobiotechnologyHuazhong Agricultural UniversityWuhan 430070China College of Science Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYangling 712100China College of Chemistry and Life Sciences Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological ResourcesChengdu Normal UniversityChengdu 611130China Yangjiang Institute of Agricultural Sciences Yangjiang 529500China

Plant diseases cause enormous economic losses in agriculture and threaten global food security,and application of agrochemicals is an important method of crop disease *** of disease-resis-tance mechanisms and synthesis of highly bioactive agrochemicals are thus important research ***,we show that propranolol,a phosphatidate phosphatase(Pah)inhibitor,effectively suppresses fungal growth,sporulation,sexual reproduction,and infection of diverse *** MoPah1 enzyme activity of the rice blast fungus Magnaporthe oryzae is inhibited by *** in lipid metabolism are associated with inhibited hyphal growth and appressorium formation caused by propranolol in *** inhibits a broad spectrum of 12 plant pathogens,effectively inhibiting infection of barley,wheat,maize,tomato,and *** improve antifungal capacity,we synthesized a series of propranolol derivatives,one of which shows a 16-fold increase in antifungal ability and binds directly to *** and its derivatives can also reduce the severity of rice blast and Fusarium head blight of wheat in *** together,our results demonstrate that propranolol suppresses fungal development and infection through mechanisms involved in lipid *** and its derivatives may therefore be promising candidates for fungicide development.

关键词： propranolol molecular design chemical modification virulence lipid metabolism Pyricularia oryzae

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Droughts and windstorms due to climate change increase variability in species and trait composition of a subtropical monsoon evergreen broadleaf forest in China

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Forest Ecosystems 2025年第1期12卷 11-19页

作者： Wenjing Chen Lei Liu Daijun Liu Josep Penuelas Guoyi Zhou Zhen Yu Anchi Wu Zhurong Wu Langqin Hua School of Ecology and Applied Meteorology Nanjing University of Information Science&TechnologyNanjing210044China Department of Botany and Biodiversity Research University of ViennaRennweg 141030ViennaAustria CSIC Global Ecology Unit CREAF-CSIC-UAB08193BellaterraBarcelonaCataloniaSpain CREAF Cerdanyola del Valles08193BarcelonaCataloniaSpain Institute of Ecology School of Ecology and Applied MeteorologyNanjing University of Information Science&TechnologyNanjing210044China Key Laboratory of Ecosystem Carbon Source and Sink of China Meteorological Administration(ECSS-CMA) Nanjing University of Information Science&TechnologyNanjing210044China Hubei Key Laboratory of Biologic Resources Protection and Utilization Hubei Minzu UniversityEnshi445000China

Background:Climate change is accelerating alterations in forest species and community composition worldwide,especially following extreme events like severe droughts and *** these effects on subtropical forests is crucial for conservation and forest management,but it remains unclear whether the impacts are stochastic or ***:We analyzed a unique dataset from a 1-ha permanent plot in a subtropical monsoon broadleaf evergreen forest in China,monitored over 26 years with six surveys from 1994 to *** forest has been free from anthropogenic disturbances for over 400 *** each survey,we measured all trees with a diameter at breast height(DBH)≥1 cm,and recorded 11 plant functional traits relating to photosynthesis,wood properties,water use,and nutrient *** this data,we calculated species and trait dispersion,assessing short-term(~5years)and long-term(26 years)trends in species and trait composition following severe droughts and windstorm ***:Severe droughts,and subsequent droughts,increased both species and trait dispersion,while species composition converged,and trait dispersion remained relatively stable throughout the recovery *** events led to increased species dispersion but decreased trait *** observed a clear directional shift in both species and trait composition under these climatic stressors,with a more pronounced increase in trait dispersion compared to species ***:In the short term(~5 years),severe droughts and windstorms increased species composition divergence,while trait composition responses *** 26 years,deterministic processes mainly drove community composition changes,especially for trait composition,although stochastic processes also played a *** findings suggest enhancing forest resilience to climatic stressors by protecting adaptive species or increasing species diversity in management practices.

关键词： Community dynamics Functional traits Community composition Deterministic processes Climate change

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Entomopathogenic fungi-based silver nanoparticles: a potential substitute of synthetic insecticides to counter behavioral and physiological immunity in Aedes aegypti mosquito (Diptera: Culicidae)

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Environmental Science and Pollution Research 2024年第21期31卷 30793-30805页

作者： Mehmood, Nasir Hassan, Ali Zhong, Xueshan Zhu, Yongzhang Ouyang, Guang Raza, Taqi Zia, Samta Chen, Xiaomin Huang, Qiuying Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory Huazhong Agricultural University Wuhan430070 China Yuexiu Center for Disease Control and Prevention Guangdong Province Guangzhou510055 China Guangzhou Yongliang Environmental Protection Technology Service CO. LTD Guangdong Province Guangzhou510405 China Wuhan Center for Disease Control and Prevention Hubei Province Wuhan430070 China

Excessive use of synthetic insecticides has resulted in environmental contamination and adverse effects on humans and other non-target organisms. Entomopathogenic fungi offer eco-friendly alternatives;however, their application for pest control requires significant advancement owing to limitations like slow killing time and effectiveness only when applied in higher amounts, whereas exposure to UV radiation, high temperature, and humidity can also reduce their viability and shelf-life. The nanoparticles synthesized using fungal extracellular extracts provide a new approach to use fungal pathogens. Our study focused on the synthesis of Metarhizium anisopliae–based silver nanoparticles (AgNPs) and evaluation of their efficiency on various physiological and behavioral parameters of the mosquito Aedes aegypti. The synthesis, size (27.6 ***, PDI = 0.209), zeta potential (− 24.3 mV), and shape of the AgNPs were determined through dynamic light scattering, scanning and transmission electron microscopic, and UV-visual spectroscopic analyses (432 nm). Our results showed significantly reduced survival (100% decrease in case of 3.2 and 1.8 μL/cm2 volumes, and 60% decrease in case of 0.8 μL/cm2 volume), phenoloxidase activity (t = 39.91;p = 0.0001), and gut microbiota, with increased oxidative stress and cell apoptosis in AgNPs-challenged mosquitoes. Furthermore, the AgNPs-exposed mosquitoes presented a concentration-specific decrease in flight locomotor activity (F = 17.312;p © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.

关键词： Silver nanoparticles

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Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis

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Insect Science 2022年第1期29卷 139-150页

作者： Qiao Gao Bei Li Bing-Xin Wei Wen Liu Pan Wang Jia-Lu Wang Xing-Miao Zhou Xiao-Ping Wang Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Department of Plant Protection Wuhan Vegetable Research InstituteWuhan430065China

Juvenile hormone (JH) absence induces photoperiod-mediated reproductive diapause, which is characterized by reproductive cessation. Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female reproduction has been well documented, its role in male reproduction remains unclear. In this study, we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis (Pallas). In a previous study, we found that adult male H. axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development, but we were unable to identify the regulators of male reproductive diapause. In this study, we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod. Additionally, application of the JH analog (JHA) methoprene promoted IRS development. Furthermore, Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod, thereby slowing IRS development. Moreover, exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown. These results indicate that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway.

关键词： Harmonia axyridis juvenile hormone male reproductive diapause methoprene-tolerant photoperiod RNAi

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An insight on the mechanism of efficient leaching of vanadium from vanadium shale induced by microwave-generated hot spots

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International Journal of Minerals,Metallurgy and Materials 2023年第2期30卷 293-302页

作者： Sheng Li Yimin Zhang Yizhong Yuan Pengcheng Hu School of Resource and Environmental Engineering Wuhan University of Science and TechnologyWuhan 430081China State Environmental Protection Key Laboratory of Mineral Metallurgical Resources Utilization and Pollution Control Wuhan University of Science and TechnologyWuhan 430081China Collaborative Innovation Center of Strategic Vanadium Resources Utilization Wuhan 430081China Hubei Provincial Engineering Technology Research Center of High Efficient Cleaning Utilization for Shale Vanadium Resource Wuhan University of Science and TechnologyWuhan 430081China School of Resource and Environmental Engineering Wuhan University of TechnologyWuhan 430070China

Microwave heating can rapidly and uniformly raise the temperature and accelerate the reaction *** this paper,microwave heating was used to improve the acid leaching,and the mechanism was investigated via microscopic morphology analysis and numerical simulation by COMSOL Multiphysics *** effects of the microwave power,leaching temperature,CaF_(2) dosage,H_(2)SO_(4) concentration,and leaching time on the vanadium recovery were investigated.A vanadium recovery of 80.66%is obtained at a microwave power of 550 W,leaching temperature of 95℃,CaF_(2) dosage of 5wt%,H_(2)SO_(4) concentration of 20vol%,and leaching time of 2.5 *** with conventional leaching technology,the vanadium recovery increases by 6.18%,and the leaching time shortens by 79.17%.More obvious pulverization of shale particles and delamination of mica minerals happen in the microwave-assisted leaching *** simulation results show that the temperature of vanadium shales increases with an increase in electric field(E-field).The distributions of E-field and temperature among vanadium shale particles are relatively uniform,except for the higher content at the contact position of the *** analysis results of scaleup experiments and leaching experiments indicate high-temperature hot spots in the process of microwave-assisted leaching,and the local high temperature destroys the mineral structure and accelerates the reaction rate.

关键词： vanadium shale microwave-assisted leaching hot spots numerical simulation

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Comparative analysis and phylogenetic and evolutionary implications of mitogenomes of Chinese Sinocyclocheilus cavefish(Cypriniformes:Cyprinidae)

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Zoological Research 2023年第4期44卷 779-781页

作者： Wan-Sheng Jiang Jie Li Hong-Mei Xiang Chao Sun Jian-Bo Chang Jun-Xing Yang Hunan Engineering Laboratory for Chinese Giant Salamander’s Resource Protection and Comprehensive Utilization and Key Laboratory of Hunan Forest Products and Chemical Industry EngineeringJishou UniversityZhangjiajieHunan 427000China College of Biology and Environmental Sciences Jishou UniversityJishouHunan 416000China Hubei Fisheries Science Research Institute WuhanHubei 430071China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China State Key Laboratory of Water Resources Engineering and Management School of Water Resources and Hydropower EngineeringWuhan UniversityWuhanHubei 430072China

DEAR EDITOR,Cavefish are of considerable interest due to the remarkable morphological changes that occurred during their adaptation to cave or subterranean river *** changes are thought to involve trade-offs in metabolic requirements and energy *** play a vital role in oxygen use and energy metabolism;thus,mitochondrial genes are likely to have experienced specific selective pressures during cavefish *** this study,we assembled and annotated the mitogenome of Sinocyclocheilus sanxiaensis,a typical cavefish species in *** this sequence and other available mitogenomes from the NCBI database,we reconstructed an updated phylogeny of Sinocyclocheilus based on 29 *** species groups were revealed,with the recently erected *** species group *** determine whether divergent selective pressures have acted on the protein-coding genes(PCGs)of the 29 Sinocyclocheilus species exhibiting different morphological features(including eye shape and body color),the fish were classified into three groups according to their cave-related *** branch model test revealed higher nonsynonymous/synonymous substitution ratios(ω)in the cave-dwelling groups,potentially resulting from a balance between adaptive selection and relaxed functional *** analysis using site and branch-site models identified 24 highly credible positive selection sites located on nine PCGs,with most sites also showing radical changes in amino acid *** study sheds light on the phylogeny and mitogenomic evolution of Sinocyclocheilus cavefish,highlighting their successful adaptation and survival in subterranean environments.

关键词： fish phylogeny habitat

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Combining Transcriptome and Metabolome Analyses to Reveal the Response of Rheum Officinale Baill. To Smut Disease and Preliminary Exploration of Ronpr1 Gene Function

SSRN

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SSRN 2025年

作者： Yang, Yuying Duan, Yuqing Luo, Yuan Jiang, Xiaogang Wang, Hua Liu, Haihua You, Jinwen Zhang, Meide Zhou, Wuxian Shandong Agricultural University China Key Laboratory of Biology and Cultivation of Herb Medicine Ministry of Agriculture and Rural Affairs Institute of Chinese Herbal Medicine Hubei Academy of Agricultral Science Enshi China Hubei Key Laboratory of Biologic Resources Protection and Utilization Hubei Minzu University Enshi China

The rampant outbreak of smut disease has led to a sharp decline in the yield and quality of Rheum officinale Baill. (R. officinale), so research on the molecular mechanisms and regulatory pathways of smut disease is urgent. After long-term observation and screening, we have identified three-year-old healthy plants that has not yet developed disease in the infected area, and selected infected plants as the control. Combining transcriptome and metabolome analysis to identify differential genes and metabolites in healthy and disease susceptible materials. These results indicated that the occurrence of smut disease has a significant impact on the secondary metabolites of R. officinale. At the same time,we identified a broad-spectrum disease resistant gene and named it RoNPR1. Bioinformatics analysis revealed that its structural domains and protein functions are extremely conserved. The RoNPR1 gene is located in the nucleus and expressed in various tissues. The occurrence of smut induces a high accumulation of RoNPR1 gene in stems, leaves, and petioles. As the invasion of smut on stems deepens, the expression level of RoNPR1 gene increases more significantly from stage I to stage V. During the stage of V to VIII, the expression level of RoNPR1 gene gradually decreased, indicating that smut disease may have caused serious damage to the growth of R. officinale. Through yeast hybridization, we verified that RoNPR1 interacts physically with RoTGA1, RoTGA2, RoTGA3, RoTGA5, and RoTGA8. In R. officinale, RoNPR3 and RoNPR4 have high functional similarity with RoNPR1 and also play a role in the occurrence of smut disease. These results reveal the changes of the secondary metabolite biosynthesis in infected R. officinale and help to further understand the expression profiles of RoNPR1 gene in response to smut disease and provide theoretical basis for breeding new resistant varieties. © 2025, The Authors. All rights reserved.

关键词： Transcription

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