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Double-stranded anchored "DNA seesaw" nanodevices for ultrasensitive photoelectrochemical biosensing in complex aquatic environments

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Sensors and Actuators B: Chemical 2025年 438卷

作者： Li, Yishuang Liu, Xinyi Chen, Guixiang Liu, Xingyu Li, Xin Lai, Guosong Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

Due to the intricate composition of environmental matrices and the inherent thermodynamic instability of single-stranded DNA, conventional photoelectrochemical (PEC) aptamer sensors, which employ DNA structure transition as signal transduction unit, exhibit significant susceptibility to environmental interference. In this study, a double-stranded anchored DNA seesaw nanodevices integrated PEC sensor was ingeniously designed to promote the ultrasensitive detection of antibiotic residues exposed in complex aquatic environments. Two different types of DNA seesaws nanodevices (with and without double stranded functionalization) were designed to validate the role of double-stranded anchoring in elevating the stability and efficiency of PEC signal transduction. Comparative results reveal that the PEC sensor equipped with double-stranded DNA-anchored nanodevices exhibits superior quantitative performance for chloramphenicol (CAP) residue with a wider linear range of 0.001–100 ng mL−1 and a lower detection limit of 0.027 pg mL−1. Density functional theory (DFT) calculations systematically demonstrate the mechanism by which DNA seesaw nanodevices regulate PEC signal transduction. Commercial enzyme-linked immunosorbent assay (ELISA) kits and spiking recovery experiments in actual aquatic samples were employed to investigate the promising detection accuracy and the favorable practicability of the fabricated sensors. We believe this study might provide new insights for the construction of more stable and efficient PEC sensors using DNA nanodevices as signal unit. © 2025 Elsevier B.V.

关键词： Signal transduction

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Synthesis of Semaxanib-like 3-alkylideneoxindoles and their binding investigation with ctDNA by multiple methods

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Journal of Molecular Liquids 2025年 419卷

作者： Li, Xiao-Yun Gao, Ruo-Hui Zhu, Jie Yang, Xiao-Hong Wang, Yu-Jie Jiang, Yu-Han Wang, Xue-Chao Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

Compounds with 3-alkylideneoxindole units are common seen in natural products, medicines and active molecules. Here, three Semaxanib-like 3-alkylideneoxindole derivatives were designed, synthesized, and characterized by 1H NMR, 13C NMR, HRMS and FT-IR. And their binding information with ctDNA were investigated by multiple methods including UV spectroscopy, fluorescence spectroscopy, 1H NMR, viscometric method and MOE-docking. Fluorescence competition experiments reflected that all of these compounds bound to the groove region of ctDNA with the binding constants 104 by orders of magnitude. Minor alterations were observed from viscometric method, which also indicated that all compounds interacted with ctDNA in a grooved manner. UV spectroscopy and 1H NMR further proved the above conclusion. Furthermore, MOE docking provided visualization of the binding results, and demonstrated that hydrogen bonds play a crucial role in the binding between the three compounds and ctDNA. This study offered detailed information about 3-alkylideneoxindole compounds binding to ctDNA, and provided valuable reference for more fully developing of drugs with 3-alkylideneoxindole units. © 2024 Elsevier B.V.

关键词： Fluorescence spectroscopy

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The Interaction Mechanism of Ziprasidone With Calf Thymus DNA by Multiple Spectrums And Molecular Docking

SSRN

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SSRN 2025年

作者： Li, Xiao-Yun Wang, Xue-Chao Gao, Ruo-Hui Chen, Xiao-Juan Lu, Jing-Jing Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

Ziprasidone (Zi) is a 2-indolone drug that has been recommended as first-line medication for schizophrenic treatment by major guides of China and America. In this paper, the binding mode of Zi and calf thymus (ctDNA) was investigated for the first time. Fluorescence spectrum studies preliminary verified that Zi binding to groove region of ctDNA. UV-vis spectra showed obvious hyperchromicity at 260 nm for ctDNA-Zi system compared to the absorption sum of ctDNA and Zi, which also could be concluded that the interaction mode of Zi to ctDNA was probably groove binding. In 1H NMR of Zi, the addition of ctDNA had no influence on its spectrum, and the viscosity of ctDNA was also unaffected by the addition of Zi. These phenomenon proved once again that Zi interacted with ctDNA at groove region. Besides, molecular docking results reflected that Zi indeed binding to minor groove region of ctDNA with hydrogen bonds as main acting force. This study elaborated detailedly the groove binding mode of antipsychotic drug Zi interacting with ctDNA, and provided effective information for the design and development of new-type antipsychotic drugs. © 2025, The Authors. All rights reserved.

关键词： Molecular docking

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Rapid, visual and autocatalytic quantifying Ag(I) and Fe(Ⅲ) by ratiometric fluorescence sensor of N, Si, S-GQDs/OPD

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Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 2025年 340卷 126366页

作者： Zhou, Rui Dong, Miao Wu, Yiwei Hubei Collaborative Innovation Center for Rare Metal Chemistry Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

N, Si, S co-doped graphene quantum dots (N, Si, S-GQDs) were synthesized from waste toner and glutathione (GSH) via a one-pot hydrothermal method. Combined with o-phenylenediamine (OPD), a rapid, visual, and autocatalytic ratiometric fluorescence sensor of N, Si, S-GQDs/OPD was fabricated for detecting Ag(I) and Fe(III) ions by producing 2,3-diaminophenazine (oxOPD) under pH 8, which emits yellow fluorescence at 560 nm while quenching the blue fluorescence of N, Si, S-GQDs at 440 nm due to an inner filter effect. With the increase of Ag(I)/Fe(III), the blue fluorescence of N, Si, S-GQDs at 440 nm was gradually weakened, along with the enhancement of yellow fluorescence at 560 nm. Hence, this color change from blue to yellow under UV light enables semi-quantitative visual detection. The sensor demonstrates high sensitivity with detection limits of 0.016 µg mL−1 for Ag(I) and 0.010 µg mL−1 for Fe(III), and it successfully detects these ions in lake and tap water without pretreatment. The autocatalytic mechanism involves Ag(I) and Fe(III) reduction to Ag nanoparticles and Fe(II), respectively, which further catalyze the reaction, enhancing selectivity and efficiency. The method is cost-effective, simple, and suitable for on-site environmental monitoring. © 2025 Elsevier B.V.

关键词： Flow visualization

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Molecular mechanism of the enhancing of AIE effect by human serum albumin

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Sensors and Actuators B: Chemical 2025年 440卷

作者： Li, Jia-Yu Lin, Wen Li, Jun-Rong Li, Tian-Hua Qin, Si-Xiang Bai, Ai-Min Yin, Miao-Miao Huang, Xiao-Huan Ding, Xin Hu, Yan-Jun Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

To address the challenge of effective aggregation of aggregation-induced emission (AIE) molecules in biological systems, the study selected four AIE molecules with the same electron acceptor but different electron donors and alkyl side chains, and employed fluorescence spectroscopy, molecular docking techniques and electrochemical analysis to explore systematically the interaction between AIE molecules and HSA and elucidate the key role of HSA in activating AIE fluorescence and its impact on AIE effects. The research has shown that these AIE molecules are unstable and emit weak light in Tris-HCl buffer solution due to their propeller-like flexible structure, but their fluorescence intensity significantly increases by 40–309 times when combined with HSA. In addition, the binding products of AIE molecules and HSA exhibit excellent biocompatibility and can achieve high-resolution imaging of cells and kidneys. These findings not only enhance our understanding of the in vivo luminescence mechanism of AIE materials but also provide an experimental basis for the application of AIE materials in biological imaging technology. © 2025 Elsevier B.V.

关键词： Biocompatibility

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Nanosheet-on-nanosheet architecture-based CNS-MnO2 composite electrode for high-performance capacitive desalination

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Materials Today Communications 2025年 46卷

作者： Tang, Xinhuyue Wang, Hongchu Ma, Siyao Zhao, Yan Kong, Xinyu Xu, Yingsheng Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

Capacitive deionization (CDI) has emerged as a promising technological advancement in desalination, aiming to alleviate the global water resource crisis by producing fresh water. Manganese dioxide (MnO2) is recognized as a potential CDI electrode material attributed to its unique crystal structure and high redox activity. However, a significant limitation arises from the stacking and agglomeration of MnO2 particles during electrochemical processes. In this work, a MnO2 composite (CNS-MnO2) featuring a nanosheet-on-nanosheet architecture was synthesized, involving the pyrolysis of inexpensive raw material sodium dihydrogen citrate to create a carbon nanosheet (CNS) substrate, followed by a hydrothermal reaction to grow MnO2 nanosheets on the carbon substrate. The CNS-MnO2 electrode exhibited notable redox activity, achieving a maximum discharging capacity of 212 F g−1 at 2 A g−1. Desalination tests revealed a maximum adsorption capacity of 116.9 mg g−1 in a 200 mg L−1 NaCl solution at 1.2 V, with impressive stability characterized by 93.5 % retention of initial capacity over 100 cycles. Furthermore, performance comparison and impedance analyses among CNS, MnO2, and CNS-MnO2 illustrated the superiority of nanosheet-on-nanosheet architecture. Therefore, this work provides in-depth guidance for developing MnO2-based electrodes achieving high-capacity desalination. © 2025 Elsevier Ltd

关键词： Desalination

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3D Fe–MOFs mimick peroxidase to enable monitoring of ascorbic acid in rat brain following brain ischemia

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Microchemical Journal 2025年 213卷

作者： Chen, Jian-Mei Wang, Yu-Xin Zhou, Neng-Jing Ding, Yong-Qi Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi 435002 China

The rational development of efficient nanozymes is important for construction of biosensors with high catalytic properties, and thus multifunctional structure and performance of metal organic frameworks (MOFs) with enzyme-like activity make them potential candidates. Herein, we prepared regular 3D concave octahedral Fe–MOFs nanozymes by a one-step solvothermal strategy, exhibiting excellent peroxidase-like catalytic activity. Mechanistic studies revealed that the peroxidase-like catalytic process in Fe–MOF involves H₂O₂ adsorption (H₂O_2ad) and rapid electron transfer at open Fe^II-O-Fe metal sites to generate hydroxyl radicals (^[rad]OH), which catalyze the oxidation of TMB to the blue product TMBox. Moreover, a platform for the quantitative detection of ascorbic acid (AA) was developed based on the good catalytic activity and stability of Fe–MOFs nanozymes. The constructed colorimetric platform for Fe–MOFs has increased sensitivity for AA measurements, possessing a good linear range of 0.01–50 µM with detection limit of about 4.5 nM. Furthermore, the ability of Fe–MOFs nanozymes to be used for facile colorimetric visualization of cerebral AA in rat following brain ischemia procedure illustrates promising applications of this platform. © 2025

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A Novel Biscoumarin Fluorescent Probe: Synthesis, Crystal Structure and Hydrazine Sensing Properties

SSRN

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SSRN 2025年

作者： Sheng-Li, Hu Luo, Mei-Ling Chen, Xin-Peng Min, Wei-Yi Liu, Yi-Kang Pan, Ji-Cheng Hubei Key Laboratory of Pollutant Analysis & Reuse Technology Department of Chemical and Enviromental Engineering Hubei Normal University Huangshi435002 China School of Life Science Hubei Normal University Hubei China Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization Hubei Normal University Huangshi435002 China

A novel biscoumarin-derived turn-on fluorescent probe for hydrazine detection has been developed and its structure has been verified by X-ray crystallography. The probe shows excellent specificity for hydrazine and its sensing mechanism was confirmed by NMR, MS and DFT calculations. Furthermore, the probe has demonstrated practical utility in quantitatively detecting hydrazine in water samples and imaging hydrazine in living cells. © 2025, The Authors. All rights reserved.

关键词： Probes

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O-phenylenediamine triggered two-step sequential fluorescence-enhanced sensor for speciation of Cr(VI)/Cr(III) in red wine/waters and photoreduction of Cr(VI)

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Food Chemistry 2025年 486卷 144601页

作者： Li, Jiahui Wu, Yiwei Hubei Collaborative Innovation Center for Rare Metal Chemistry Hubei Key Laboratory of Pollutant Analysis & Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China

Speciation of Cr(VI)/Cr(III) and degradation of Cr(VI) with high toxicity to Cr(III) are required. We constructed a technique of o-phenylenediamine (OPD) triggered two-step sequential fluorescence-enhanced sensor for speciation of Cr(VI)/Cr(III) and photoreduction of Cr(VI). With Fe[sbnd]N chelation between Fe3O4 QDs and OPD, the fluorescence intensity of Fe3O4 QDs was not only enhanced by 1.5 times, but that of Fe3O4 QDs/OPD sensor further increased by Cr(VI), and showed no response to Cr(III). Hence, OPD is the key triggered factor for establishing the sensor, and the detection limit of Cr(VI) by Fe3O4 QDs/OPD sensor was 2.44 μg L−1. Furthermore, under the irradiation at 385 nm, Fe3O4 QDs were activated and generated e−, which played a leading role in the reduction of Cr(VI) to Cr(III). The reduction efficiency of Cr(VI) was over 99 % within 2 h. This work provides an efficient, green and convenient method for rapid monitoring and treatment of Cr(VI). © 2025 Elsevier Ltd

关键词： Chelation

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Smartphone-Integrated Colorimetric Sensor Based on Cecu–Mofs Nanozymes with Enhanced Triple-Enzyme Mimetic Activity

SSRN

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SSRN 2025年

作者： Ding, Yongqi Chen, Jianmei Zhou, Nengjing Huang, Xin Sun, Zhongyu Hubei Key Laboratory of Pollutant Analysis and Reuse Technology College of Chemistry and Chemical Engineering Hubei Normal University Huangshi435002 China School of Chemistry and Materials Science Jiangsu Normal University 101 Shanghai Road Xuzhou221116 China

Metal-organic frameworks (MOFs) are often acted as potential candidates for the construction of efficient nanozymes due to their unique metal-ligand assembly structure, which can mimic different enzyme activities by modulating the metal nodes and ligand environment. In this study, a three-in-one composite metal-organic framework nanozymes (CeCu–MOFs) with excellent intrinsic peroxidase, oxidase and catalase-like catalytic properties have been successfully prepared in environmentally friendly manner by a one-step chemical deposition method. Meanwhile, a catalytic platform for signal amplification was designed for quantitative measurement of ascorbic acid (AA) using oxidase-like characteristic of CeCu–MOFs. The constructed colorimetric sensor achieved selective identification and accurate measurement of AA, demonstrated good linearity in the range of 1-50 μM with detection limit of 0.063 μM, and further developed a portable smartphone platform for visual colorimetric analysis of AA in real samples in point-of-care (POC) fashion by utilizing a portable paper-based device. © 2025, The Authors. All rights reserved.

关键词： Ligands

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