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Environmental Functional Materials 2025年

作者： Aqib Zahoor Xiao Liu Yuxin Liu Shengyong Liu Wang Yi Shahdev Sajnani Lingyu Tai Nadeem Tahir Boré Abdoulaye Mahaveer Yuan Liu Zia ur Rahman Martina Damizia Benedetta de Caprariis International Joint Research Center for the Control and Prevention of Environmental Pollution on Tropical lslands of Hainan Province Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province School of Environmental Science and Engineering Hainan University Haikou 570228 P. R. China Collaborative Innovation Center of Biomass Energy Henan Agricultural University Zhengzhou 450002 School of Environmental Science and Engineering Tianjin University Tianjin 300350 P. R. China Faculty of Civil and Environmental Engineering Gdańsk University of Technology ul. Narutowicza 11/12 80-233 Gdańsk Poland Interdisciplinary Research Center for Refining and Advanced Chemicals King Fahd University of Petroleum & Minerals Dhahran 31261 Saudi Arabia Department of Chemical Engineering Materials and Environment Sapienza University of Rome Via Eudossiana 18 00184 Rome Italy

Globally, roughly 380 trillion liters of wastewater are generated annually, with a significant portion remaining untreated due to expensive treatment and lack of expertise, consequently, leading severe environmental and public health problems. This research aims to identify the most environmentally sustainable and eco-friendly approach to wastewater treatment. Results shows that, agricultural lignocellulose-based biomass (ALCB) biochar is highly effective for wastewater treatment due to its abundance, cost-effectiveness, and excellent adsorption properties. The ability of sustaining high carbon content makes it a valuable carbon sink beneficial for the carbon reduction. Annually, ALCB is widely available as a byproduct in developing countries like China 716 (million tons) Mt, India 605 Mt, Brazil 451 Mt, Pakistan 285 Mt as well as in developed regions U.S.A 682 Mt, and Europe 580 Mt. Pyrolysis, gasification, and torrefaction are mostly used methods for ALCB biochar production. Physicochemical properties including surface area, porosity, functional groups, pH, and elemental composition notably affected by production methods, feedstock types and reactor designs determine the adsorption performance and environmental safety of ALCB biochar. The adsorption mechanisms of the organic and inorganic pollutant on biochar are ion exchange, complexation, electrostatic attraction, pore-filings, and physical adsorption, etc. LCA assessment revealed that the utilization of ALCB biochar on wastewater treatment offers several economic, environmental, and educational benefits through the aspect of waste management, revenue generation, job creations, pollution control, trading and skill developments.

关键词： Agricultural lignocellulose-based biochar Pyrolysis Pollutant adsorption Sustainable water treatment Environmental and economic benefits

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Advanced converter collaborative control method based on smart contract

Advanced converter collaborative control method based on sma...

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The 11th IET International Conference on Advances in Power System control, Operation and Management (APSCOM 2018)

作者： Kaihua Xin Bin Duan Yinxin Yan Hunan Province Engineering Research Center for Multi-Energy Collaborative Control Technology Hunan Province Cooperative Innovation Center for Wind Power Equipment and Energy Conversion College of Information Engineering Xiangtan University Xiangtan Hunan 411105 China

ISBN: (数字)9781839530043

In this paper, a cooperative control method of advanced converter using smart contract is proposed in the Micro-grid with uncertain renewable energy output. The problem of power quality in Micro-grid is solved by using the active operation decision of the microgrid converter as the goal. First, the information interaction mechanism and model between the central Micro-grid converters is proposed in this paper. When the different strategies in the distribution network are switched, the real-time transaction requests can be initiated to other converters in the Micro-grid, and the disturbance of the power quality can be eliminated by the other converters in Micro-grid, and the dynamic balance of the power quality of the Micro-grid can be maintained. Then, a Vickrey-Clarke-Groves(VCG) auction method was introduced to encourage proprietor bidding of her neighbors, and the mechanism of distributed decision making and cooperative autonomous operation is realized by using smart contract. Among them, smart contract is signed between the various converters. On the one hand, the update of the state in the control cycle is realized, which makes the converter take more responsibilities and participate in the power quality control. To a certain extent, the effectiveness of the method is ensured. In addition, rewards and penalties are given according to the achievement of the smart contract between the converters, which reduces the default rate of the owners. This degrading transaction process improves the security and completeness of the transaction. It provides more means to solve the power quality of the power grid and ensures the safe and reliable operation of the distribution network.

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research on Characteristics of Morphological Filters

Research on Characteristics of Morphological Filters

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第二届电气、控制与自动化国际会议

作者： Fang Xie Kangkang Liang Wenming Wu Wenjie Hong Wei Hu School of Electrical Engineering and Automation Anhui University National Engineering Laboratory of Energy-Saving Motor & Control Technology Anhui University Engineering Research Center of Power Quality Ministry of Education Anhui University Anhui Province Laboratory of Industrial Energy-Saving and Safety Anhui University Collaborative Innovation Center of Industrial Energy-Saving and Power Quality Control Anhui University

As a kind of non-linear filter, morphology filter is widely applied in sampled power system single processing and motor control with its effective denoising performance. If the structural elements are selected properly, the morphological filter can do better in maintaining the original characteristics of the signal. However, in practical applications, it is a major difficulty to grasp the factors that affect the cut-off frequency of morphological filters. This paper will solve these problems. In this paper, the frequency response characteristic of the combined morphological filter is presented by excitation signal inputted into the given morphological filter. The research indicates when the sampling frequency is fixed, the cut-off frequency increases with the increase of the length of the structural elements. The cut-off frequency gets smaller as the width of structure element grows, and the relationship between them is nonlinear. When the width of the structure element is fixed, as the sampling frequency changed, the cut-off frequency varies directly with the sampling frequency. Experiments show the corresponding rule of morphological filter frequency.

关键词： filter research on Characteristics of Morphological Filters

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二维材料最新研究进展

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物理化学学报 2021年第12期37卷 1-151页

作者：常诚陈伟陈也陈永华陈雨丁峰樊春海范红金范战西龚成宫勇吉何其远洪勋胡晟胡伟达黄维黄元季威李德慧李连忠李强林立凌崇益刘鸣华刘楠刘庄 Kian Ping Loh 马建民缪峰彭海琳邵明飞宋礼苏邵孙硕谭超良唐智勇王定胜王欢王金兰王欣王欣然 Andrew T.S.Wee 魏钟鸣吴宇恩吴忠帅熊杰熊启华徐伟高尹鹏曾海波曾志远翟天佑张晗张辉张其春张铁锐张翔赵立东赵美廷赵伟杰赵运宣周凯歌周兴周喻朱宏伟张华刘忠范 Institute of Science and Technology Austria Am Campus 13400 KlosterneuburgAustria Department of Chemistry National University of Singapore3 Science Drive 3117543Singapore Department of Chemistry The Chinese University of Hong KongShatinNew TerritoriesHong KongChina Key Laboratory of Flexible Electronics and Institute of Advanced Materials Nanjing Tech UniversityNanjing 211816China School of Life Sciences Shanghai UniversityShanghai 200444China Centre for Multidimensional Carbon Materials Institute for Basic ScienceUlsan 44919Korea School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Physical and Mathematical Sciences Nanyang Technological UniversitySingapore 637371Singapore Department of Chemistry City University of Hong KongKowloonHong KongChina Department of Electrical and Computer Engineering and Quantum Technology Center University of MarylandCollege ParkMaryland 20742USA School of Materials Science and Engineering Beihang UniversityBeijing 100191China Department of Materials Science and Engineering City University of Hong KongKowloonHong KongChina Center of Advanced Nanocatalysis(CAN) Hefei National Laboratory for Physical Sciences at the MicroscaleDepartment of Applied ChemistryUniversity of Science and Technology of ChinaHefei 230026China College of Chemistry and Chemical Engineering State Key Laboratory of Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy Materials(iChEM)Xiamen UniversityXiamen361005Fujian ProvinceChina State Key Laboratory of Infrared Physics Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing100081China Beijing Key Laboratory of Optoelectronic Functional Materials&Micro-Nano Devices Department of PhysicsRenmin University of ChinaBeijing 100872China School of Optical and Electronic Information Wuhan National Laboratory

research on two-dimensional(2D) materials has been explosively increasing in last seventeen years in varying subjects including condensed matter physics, electronic engineering, materials science, and chemistry since the mechanical exfoliation of graphene in 2004. Starting from graphene, 2D materials now have become a big family with numerous members and diverse categories. The unique structural features and physicochemical properties of 2D materials make them one class of the most appealing candidates for a wide range of potential applications. In particular, we have seen some major breakthroughs made in the field of 2D materials in last five years not only in developing novel synthetic methods and exploring new structures/properties but also in identifying innovative applications and pushing forward commercialisation. In this review, we provide a critical summary on the recent progress made in the field of 2D materials with a particular focus on last five years. After a brief backgroundintroduction, we first discuss the major synthetic methods for 2D materials, including the mechanical exfoliation, liquid exfoliation, vapor phase deposition, and wet-chemical synthesis as well as phase engineering of 2D materials belonging to the field of phase engineering of nanomaterials(PEN). We then introduce the superconducting/optical/magnetic properties and chirality of 2D materials along with newly emerging magic angle 2D superlattices. Following that, the promising applications of 2D materials in electronics, optoelectronics, catalysis, energy storage, solar cells, biomedicine, sensors, environments, etc. are described sequentially. Thereafter, we present the theoretic calculations and simulations of 2D materials. Finally, after concluding the current progress, we provide some personal discussions on the existing challenges and future outlooks in this rapidly developing field.

关键词： Two-dimensional materials Transition metal dichalcogenides Phase engineering of nanomaterials Electronics Optoelectronics Catalysis energy storage and conversion

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I3−-Mediated Oxygen Evolution Activities to Boost Rechargeable Zinc-Air Battery Performance with Low Charging Voltage and Long Cycling Life

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Angewandte Chemie 2024年第4期137卷

作者： Dr. Xiaohong Zou Dr. Qian Lu Lizhen Wu Kouer Zhang Mingcong Tang Haijiao Xie Prof. Xiao Zhang Prof. Zongping Shao Prof. Liang An Department of Mechanical Engineering The Hong Kong Polytechnic University Hung Hom Kowloon Hong Kong SAR PR China Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control School of Environmental Science and Technology Nanjing University of Information Science and Technology Nanjing 210044 PR China Department of Chemistry The Chinese University of Hong Kong Ma Lin building Shatin 999077 Hong Kong SAR PR China Hangzhou Yanqu Information Technology Co. Ltd. Y2 2 nd Floor Building 2 Xixi Legu Creative Pioneering Park No. 712 Wen'er West Road Xihu District Hangzhou City Zhejiang Province 310003 P.R.O.C. Research Institute for Advanced Manufacturing The Hong Kong Polytechnic University Hung Hom Kowloon Hong Kong SAR PR China Research Institute for Smart Energy The Hong Kong Polytechnic University Hung Hom Kowloon Hong Kong SAR PR China WA School of Mines: Minerals Energy and Chemical Engineering (WASM-MECE) Curtin University Perth WA-6102 Australia

An effective strategy to facilitate oxygen redox chemistry in metal-air batteries is to introduce a redox mediator into the liquid electrolyte. The rational utilization of redox mediators to accelerate the charging kinetics while ensuring the long lifetime of alkaline Zn-air batteries is challenging. Here, we apply commercial acetylene black catalysts to achieve an I 3 − -mediated Zn-air battery by using ZnI 2 additives that provide I 3 − to accelerate the cathodic redox chemistry and regulate the uniform deposition of Zn 2+ on the anode. The Zn-air battery performs an ultra-long cycle life of over 600 h at 5 mA cm −2 with a final charge voltage of 1.87 V. We demonstrate that I − mainly generates I 3 − on the surface of carbon catalysts during the electrochemically charging process, which can further chemically react with OH − to generate oxygen and further revert to I − , thus obtaining a stable electrochemical system. This work offers a strategy to simultaneously improve the cycling life and reduce the charging voltage of Zn-air batteries through redox mediator methods.

关键词： Zinc-air batteries Redox mediators oxygen electrocatalysts Redox chemistry Stability

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Catenary Fault Identification Based on PSO-ELM

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Journal of Physics: Conference Series 2019年第3期1302卷

作者： Lingzhi Yi Jian Zhao Wenxin Yu Yue Liu Chuyang Yi Dan Jiang Hunan Province Engineering Research Center for Multi-Energy Collaborative Control Technology Hunan Province Cooperative Innovation Center for Wind Power Equipment and Energy Conversion College of Information Engineering Xiangtan University. Xiangtan Hunan 411105 China College of Electrical and Information Engineering Hunan University Changsha Hunan 410082 China School of Information and Electrical Engineering Hunan University of Science and Technology Xiangtan Hunan 411201 China Hunan Railway Professional Technology College Zhuzhou Hunan 412000 China

Catenary has been exposed outdoors for a long term, and its failure rate is very high, which has seriously affected the operation and development of traction power supply system. Due to the problems of long detection time, backward detection means and influenced by human factors in traditional catenary fault identification methods, this paper proposed a fault identification method based on PSO-ELM. This method could reduce the hidden layer nodes of traditional ELM and improve the accuracy of identification. In this paper, this method was compared with ELM, GA-ELM, BP, GA-BP and PSO-BP. A sample of catenary detection data of a power supply section in 2018 was selected. The results show that PSO-ELM is an efficient method for the fault identification of catenary.

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2020 Roadmap on gas-involved photo-and electro-catalysis

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Chinese Chemical Letters 2019年第12期30卷 2089-2109页

作者： Yulu Yang Yang Tang Haomin Jiang Yongmei Chen Pingyu Wan Maohong Fan Rongrong Zhang Sana Ullah Lun Pan Ji-Jun Zou Mengmeng Lao Wenping Sun Chao Yang Gengfeng Zheng Qiling Peng Ting Wang Yonglan Luo Xuping Sun Alexander S.Konev Oleg V.Levin Panagiotis Lianos Zhuofeng Hu Zhurui Shen Qinglan Zhao Ying Wang Nadia Todorova Christos Trapalis Matthew V.Sheridan Haipeng Wang Ling Zhang Songmei Sun Wenzhong Wang Jianmin Ma School of Physics and Electronics Hunan UniversityChangsha 410082China College of Chemistry Beijing University of Chemical TechnologyBeijing 100029China School of Civil and Environmental Engineering Georgia Institute of TechnologyAtlantaGA 30332United States College of Engineering and Applied Sciences and School of Energy ResourcesUniversity of WyomingLaramieWY 82071United States Key Laboratory for Green Chemical Technology of the Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Collaborative Innovative Center of Chemical Science and Engineering(Tianjin) Tianjin 300072China Institute for Superconducting and Electronic Materials Australian Institute for Innovative MaterialsUniversity of WollongongNSW 2522Australia Laboratory of Advanced Materials Department of ChemistryFudan UniversityShanghai 200438China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province College of Chemistry and Chemical EngineeringChina West Normal UniversityNanchong 637002China Institute of Chemistry St.Petersburg State University7/9 Universitetskaya emb.St.Petersburg 199034Russian Federation Department of Chemical Engineering University of PatrasPatras 26500Greece School of Environmental Science and Engineering Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation TechnologySun Yat-sen UniversityGuangzhou 510275China School of Materials Science and Engineering Nankai UniversityTianjin 300350China Department of Chemistry Chinese University of Hong KongHong KongChina Institute of Nanoscience and Nanotechnology NCSR"Demokritos"Athens 15341Greece State Key Laboratory of School of Radiation Medicine and Protection and School for Radiological and interdisciplinary Sciences(RAD-X) Soochow UniversitySuzhou 215123China State Key Laboratory of High Performance Ceramics and Superf

Green reactions not only provide us chemical products without any pollution,but also offer us the viable technology to realize difficult tasks in normal ***-,photoelectro-,and electrocatalytic reactions are indeed powerful tools to help us to embrace bright ***,some gas-involved reactions are extremely useful to change our life environments from energy systems to liquid fuels and cost-effective products,such as H2 evolution(H2 production),02 evolution/reduction,CO2 reduction,N2 reduction(or N2 fixation) *** can provide fuel cells clean H2 for electric vehicles from H2 evolution reaction(HER),at the same time,we also need highly efficient 02 reduction reaction(ORR) in fuel cells for improving the reaction ***,we can get the clean oxidant O2 from water through O2 evolution reaction(OER),and carry out some reactions without posing any pollution to reaction ***,we can translate the greenhouse gas CO2 into useful liquid fuels through CO2 reduction reaction(CRR).Last but not the least,we can get ammonia from N2 reduction reaction(NRR),which can decrease energy input compared to the traditional Hubble *** reactions,such as HER,ORR,OER,CRR and NRR could be realized through solar-,photoelectro-and electro-assisted *** them,the catalysts used play crucial roles in determining the efficiency and kinds of products,so we should consider the efficiency of ***,the cost,synthetic methods of catalysts should also be ***,significant progress has been achieved,however,many challenges still exist,reaction systems,catalysts underlying mechanisms,and so *** extremely active fields,we should pay attention to *** the background,it has motivated us to contribute with a roadmap on ’GasInvolved Photo-and Electro-Catalysis’.

关键词： H2 evolution reaction O2 reduction reaction O2 evolution reaction CO2 reduction reaction N2 reduction reaction Electrocatalysis Photocatalysis Photoelectrocatalysis

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Tunable Topological energy Bands in 2D Dialkali-Metal Monoxides

arXiv

引用

arXiv 2019年

作者： Hua, Chenqiang Li, Si Xu, Zhu-An Zheng, Yi Yang, Shengyuan A. Lu, Yunhao Zhejiang Province Key Laboratory of Quantum Technology and Device Department of Physics in Zhejiang University State Key Lab of Silicon Materials School of Materials Science and Engineering in Zhejiang University Hangzhou310027 China School of Physics and Electronics Hunan Normal University ChangshaHunan410081 China Research Laboratory for Quantum Materials in Singapore University of Technology and Design Singapore487372 Singapore Center for Quantum Transport and Thermal Energy Science School of Physics and Technology Nanjing Normal University Nanjing210023 China Zhejiang Province Key Laboratory of Quantum Technology and Device Department of Physics in Zhejiang University Hangzhou310027 China Collaborative Innovation Centre of Advanced Microstructures in Nanjing University Nanjing210093 China

2D materials with nontrivial energy bands are highly desirable for exploring various topological phases of matter, as low dimensionality opens unprecedented opportunities for manipulating the quantum states. Here, it is reported that monolayer (ML) dialkali-metal monoxides, in the well-known 2H-MoS2 type lattice, host multiple symmetry-protected topological phases with emergent fermions, which can be effectively tuned by strain engineering. Based on first-principles calculations, it is found that in the equilibrium state, ML Na2O is a 2D double Weyl semimetal, while ML K2O is a 2D pseudospin-1 metal. These exotic topological states exhibit a range of fascinating effects, including universal optical absorbance, super Klein tunneling, and super collimation effect. By introducing biaxial or uniaxial strain, a series of quantum phase transitions between 2D double Weyl semimetal, 2D Dirac semimetal, 2D pseudospin-1 metal, and semiconductor phases can be realized. The results suggest monolayer dialkali-metal monoxides as a promising platform to explore fascinating physical phenomena associated with novel 2D emergent fermions. Copyright © 2019, The Authors. All rights reserved.

关键词： Phase transitions

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Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

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Nature Communications 2020年第1期11卷 2118-2118页

作者： Zhang, Mingxia Zou, Yuan Xu, Xing Zhang, Xuebing Gao, Mingxuan Song, Jia Huang, Peifeng Chen, Qin Zhu, Zhi Lin, Wei Zare, Richard N. Yang, Chaoyong State Key Laboratory of Physical Chemistry of Solid Surfaces The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation Key Laboratory for Chemical Biology of Fujian Province Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China Department of Chemistry Stanford University Stanford 94305 CA United States Hangzhou Weizhu Biological Technology Co. Ltd Hangzhou China Institute of Molecular Medicine State Key Laboratory of Oncogenes and Related Genes Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China Translational Genomics Research Institute Molecular Medicine Division Phoenix AZ United States Hunan Provincial Key Lab of Emergency and Critical Care Hunan People’s Hospital Changsha China

ScRNA-seq has the ability to reveal accurate and precise cell types and states. Existing scRNA-seq platforms utilize bead-based technologies uniquely barcoding individual cells, facing practical challenges for precious samples with limited cell number. Here, we present a scRNA-seq platform, named Paired-seq, with high cells/beads utilization efficiency, cell-free RNAs removal capability, high gene detection ability and low cost. We utilize the differential flow resistance principle to achieve single cell/barcoded bead pairing with high cell utilization efficiency (95%). The integration of valves and pumps enables the complete removal of cell-free RNAs, efficient cell lysis and mRNA capture, achieving highest mRNA detection accuracy (R = 0.955) and comparable sensitivity. Lower reaction volume and higher mRNA capture and barcoding efficiency significantly reduce the cost of reagents and sequencing. The single-cell expression profile of mES and drug treated cells reveal cell heterogeneity, demonstrating the enormous potential of Paired-seq for cell biology, developmental biology and precision medicine. © 2020, The Author(s).

关键词： Lab-on-a-chip Sequencing RNA sequencing Stem-cell differentiation

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Unveiling microbial communities with EasyAmplicon: A user-centric guide to perform amplicon sequencing data analysis

iMetaOmics

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iMetaOmics 2024年第2期1卷

作者： Salsabeel Yousuf Hao Luo Meiyin Zeng Lei Chen Tengfei Ma Xiaofang Li Maosheng Zheng Xin Zhou Liang Chen Jiao Xi Hongye Lu Huiluo Cao Xiaoya Ma Bian Bian Pengfan Zhang Jiqiu Wu Renyou Gan Baolei Jia Linyang Sun Zhicheng Ju Yunyun Gao Waqar Afzal Malik Chuang Ma Hujie Lyu Yahui Li Huiyu Hou Yuanping Zhou Defeng Bai Yao Wang Haifei Yang Jiani Xun Shengda Du Tianyuan Zhang Xiulin Wan Kai Peng Shanshan Xu Tao Wen Tong Chen Yong-Xin Liu Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen Guangdong China Department of Vascular Surgery Fu Xing Hospital Capital Medical University Beijing China State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems Centre for Grassland Microbiome College of Pastoral Agriculture Science and Technology Lanzhou University Lanzhou Gansu China Centre for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Shijiazhuang Hebei China College of Environmental Science and Engineering North China Electric Power University Beijing China State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences Beijing China Biomedical Innovation Center and Beijing Key Laboratory for Therapeutic Cancer Vaccines Beijing Shijitan Hospital Capital Medical University Beijing China College of Natural Resources and Environment Northwest A&F University Yangling Shaanxi China Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Zhejiang Provincial Clinical Research Center for Oral Diseases Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province Hangzhou Zhejiang China Department of Microbiology University of Hong Kong Pok Fu Lam Hong Kong China Center for Energy Metabolism and Reproduction Institute of Biomedicine and Biotechnology Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen Guangdong China Department of Cardiology Shenzhen Guangming District People's Hospital Shenzhen Guangdong China Department of Computational Biology and Medical Sciences Graduate School of Frontier Sciences The University of Tokyo Kashiwa Chiba Japan Innovative Genomics Institute University of California Berkeley C

The advent of next-generation sequencing has revolutionized microbiome research, enabling in-depth exploration of microbial communities through amplicon sequencing. The widespread adoption of sequencing across diverse fields, coupled with decreasing costs, underscores the critical need for validated, fully automated, reproducible, and adaptable analysis pipelines. However, analyzing these high-throughput datasets often necessitates extensive bioinformatics expertize, hindering accessibility for many researchers. To address this challenge, in 2023 we developed EasyAmplicon, a comprehensive, user-friendly pipeline that integrates popular tools such as USEARCH and VSEARCH, offering a streamlined workflow from raw data to results. Remarkably, EasyAmplicon has garnered significant recognition within a year, as evidenced by 127 citations to date. To further facilitate the researchers and enhance usability, we present a detailed protocol with a video recording that guides users through each step of the pipeline, including data preprocessing (quality filtering, chimera removal), amplicon sequence variant analysis, diversity analysis, and data visualization. The protocol is designed for ease of use, with each step documented, allowing researchers to execute the workflow without requiring complex scripting skills. The EasyAmplicon pipeline is freely available on GitHub ( https://***/YongxinLiu/EasyAmplicon ). EasyAmplicon offers a fully automated, user-friendly pipeline for comprehensive amplicon sequencing analysis, seamlessly integrating various bioinformatics tools. The pipeline supports efficient data preprocessing, diversity analysis, and advanced visualization, making high-throughput data analysis accessible to a broader range of researchers. Each step is documented, along with troubleshooting tips and solutions, for users' convenience.

关键词： computational biology EasyAmplicon metagenome microbiome

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