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The effect of continuous Ni(Ⅱ) exposure on the organic degradation and soluble microbial product(SMP) formation in two-phase anaerobic reactor

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Journal of Environmental Sciences 2015年第7期27卷 78-87页

作者： Wei Wu Tengteng Duan Hailiang Song Yan Li Ang Yu Long Zhang Aimin Li State Key Laboratory of Pollution Control and Resources Reuse School of the Environment Nanjing University Jiangsu Key Lab of Environmental Engineering Jiangsu Provincial Academy of Environmental Science School of Energy and Environment Southeast University

A two-phase anaerobic reactor fed with glucose substrate（3 g chemical oxygen demand（COD）/L） was used to investigate the effects of toxic metals on the degradation of organics and the soluble microbial product（SMP） formation. Low concentrations of Ni（II）（5 and10 mg/L） promoted the acid phase, whereas high concentrations（15, 20, and 25 mg/L）exhibited an inhibitory effect on, but did not alter the fermentative method, which mainly involved the fermentation of propionic acid. The methanogenic microorganism exhibited a strong capability adapting constantly increased Ni（II） levels. The acid phase was an accumulation stage of SMP. In the absence of Ni（II）, the high-molecular-weight material in the effluent SMP mainly contained polysaccharide, tryptophan, and casein. Methanogens metabolized most of the polysaccharide, the whole tryptophan content, and part of the casein, leading to the presence of humic acid and protein in effluent. After Ni（II） dosage, the protein and polysaccharide of the acid phase increased, and tryptophan changed, while casein remained stable. More protein than polysaccharide was produced, suggesting the prominent function of protein when addressing the negative effect of toxic metals. The analysis of DNA confirmed the change of bacterial activity.

关键词： Soluble microbial products （SMP） Two-phase anaerobic reactor Upflow anaerobic sludge bed （UASB） Ni（II）

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Four Plants' Efficiency on Restoring Uranium-containing Wastewater

Four Plants' Efficiency on Restoring Uranium-containing Wast...

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2015 International Conference on Computer Science and Environmental Engineering(CSEE 2015)

作者： L.S.Rong R.L.Yang C.Q.Dong C.Zhao Z.Yan S.B.Xie Hunan Provincial Key Laboratory of Pollution Control and Resources Technology University of South China Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy University of South China

By using hydroponics experiments,we observed the change of photosynthetic pigments and Malondialdehyde(MDA) content of Eichhornia crassipes,Pistia stratiotes,Vetiveria zizanioides and Acorus calamus Linns under different Uranium concentration(0,0.1,1,5,10,20 mg·L-1 respectively).The result showed that 0.1 mg·L-1 and 1 mg·L-1 Uranium could promote to increase the content of photosynthetic pigment,but as the concentration keptincreasing,it had opposite *** the other hand,MDA increased along with the increase of Uranium *** concentration effect of Eichhornia crassipes,Pistia stratiotes,Vetiveria *** Acorus calamus Linns increased as Uranium concentration *** four plants can be used as enrichment plant to absorb Uranium,however,Acorus calamus Linn would be a better choice comparing to other three species.

关键词： uranium enrichment plant restoring

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Responses of Seed Yield and Economic Benefit of Winter Oilseed Rape(Brassica napus L.) to Different NPK Ratio and Dose

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Agricultural Science & technology 2015年第11期16卷 2361-2366页

作者： Yingzi WANG Chang TIAN Xuan ZHOU Wenming WANG Jianwei PENG Zhenhua ZHANG Haixing SONG Chunyun GUAN College of Resources and Environment Hunan Agricultural University Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use & Hunan Provincial Key Laboratory of Plant Nutrition in Common University & National Engineering Laboratory of College of Resources and Environment College of Horticulture and Landscape Hunan Agricultural University College of Environmental Science and Resources Zhejiang University Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China Hunan Branch National Center of Oilseed Crops Improvement

Field experiments were conducted during 2008-2010 to investigate the ef- fects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape （Brassica napus L.） - Xiangzayou763, and to determine the optimum NPK ratio and dose in hunan, China. The results showed that NPK ratio （1：0.50：0.50） with 180 kg/hm2 N, 90 kg/hm2 P2O5 and 90 kg/hm2 K20 was the optimum combination for the highest seed yield （2 231.13 kg/hm2） and economic benefit （9 816.97 Yuan/hm2）, of which 1 641.23 Yuan/hm2 being set for fertilizer in- vestment （VCR=4.11）. Besides, the effects of N, P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical. N had the greatest ef- fect on plant growth of winter oilseed rape, followed by P and K. Total leaf number, green leaf number, leaf length and width, rhizome diameter, branch number, dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments. By correlation and path analysis of yield components, the contribution rate to seed yield was found： effective silique number per plant〉seed number per silique〉l 000-seed weight.

关键词： NPK ratio anddose Seed yield Economic benefit Winter oilseed rape

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Characteristics and mechanism of uranium(VI) absorbed by anaerobic granular sludge

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Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals 2014年第9期24卷 2418-2425页

作者： Chen, Hua-Bai Xie, Shui-Bo Liu, Jin-Xiang Xiao, Shi-Hong Zeng, Tao-Tao Ling, Hui Wang, Jin-Song Hunan Provincial Key Laboratory of Pollution Control and Resources Reuse Technology University of South China Hengyang421001 China

The anaerobic granular sludge from a paper mill was used to prepare powdered anaerobic granular sludge (PAGS) as a novel biological adsorbent. Experimental parameters such as pH value, adsorbent PAGS dosage, initial U(VI) concentration and adsorption time were examined to investigate their effects on the adsorption of U(VI) by PAGS. The adsorption mechanism was studied by model simulation, scanning electron microscopy, energy-dispersive spectrometry, infrared spectroscopy and ion exchange experiments. The results show that the maximum adsorption occurs at pH value of 6, adsorbent PAGS dosage of 2.0 g/L and adsorption time of 60 min for an initial U(VI) concentration of 10 mg/L and 30 ℃. The equilibrium data fit well with the pseudo-second-order model (R2≈1) and Freundlich model (R2>0.99). SEM images, EDS analysis results and ion exchange experiments verify that ion exchange is the main adsorption mechanism with Ca2+ as the main exchange ion. FTIR spectra show that complexation is also one of the adsorption mechanisms with -OH, C=O, Si-OH and -NH2 as the major functional groups involved. The used PAGS can be desorbed by 0.1 mol/L HCl (the first desorption rate is up to 99%) and can be reusable. PAGS is nontoxic and eco-friendly, which has a good prospect in the treatment of uranium-containing wastewater. ©, 2014, Science Press. All right reserved.

关键词： Desorption

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Responses of soil microbial biomass and bacterial community structure to closed-off management (an ecological natural restoration measures): A case study of Dongting Lake wetland, middle China

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Journal of bioscience and bioengineering 2016年第3期122卷 345-50页

作者： Juan Dai Haipeng Wu Chang Zhang Guangming Zeng Jie Liang Shenglian Guo Xiaodong Li Lu Huang Lunhui Lu Yujie Yuan College of Environmental Science and Engineering Hunan University Changsha 410082 PR China Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China Changjiang River Scientific Research Institute Wuhan 430072 PR China. Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: zgming@***. State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan 430072 PR China. Key Laboratory of Reservoir Aquatic Environment of CAS Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing 400714 China.

Soil microbial biomass (SMB) and bacterial community structure, which are critical to global ecosystem and fundamental ecological processes, are sensitive to anthropogenic activities and environmental conditions. In this study, we examined the possible effects of closed-off management (an ecological natural restoration measures, ban on anthropogenic activity, widely employed for many important wetlands) on SMB, soil bacterial community structure and functional marker genes of nitrogen cycling in Dongting Lake wetland. Soil samples were collected from management area (MA) and contrast area (CA: human activities, such as hunting, fishing and draining, are permitted) in November 2013 and April 2014. Soil properties, microbial biomass carbon (MBC), and bacterial community structure were investigated. Comparison of the values of MA and CA showed that SMB and bacterial community diversity of the MA had a significant increase after 7 years closed-off management. The mean value of Shannon-Weiner diversity index of MA and CA respectively were 2.85 and 2.07. The gene copy numbers of 16S rRNA and nosZ of MA were significant higher than those of CA. the gene copy numbers of ammonia-oxidizing archaea (AOA) and nirK of MA were significant lower than those of CA. However, there was no significant change in the gene copy numbers of ammonia-oxidizing bacteria (AOB) and nirS.

关键词： Bacterial community diversity Ecological management Ecological restoration Microbial biomass carbon Nitrogen Wetland

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Effect and mechanism of zero-valent iron on reducing U(VI) by S. oneidensis

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Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals 2015年第8期25卷 2309-2316页

作者： Liu, Jin-Xiang Xie, Shui-Bo Ma, Hua-Long Wang, Yong-Hua Chen, Hua-Bai Li, Biao Hunan Provincial Key Laboratory of Pollution Control and Resources Reuse Technology University of South China Hengyang421001 China Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy University of South China Hengyang421001 China

The effects and mechanism of U(VI) reduction by Shewanella oneidensis (***) MR-1 with zero-valent iron(ZVI) were evaluated. The results show that either ZVI or bacteria can reduce U(VI) in the solution, and under anaerobic conditions, a cooperative action of ZVI and the bacteria *** is observed in reducing process of U(VI). Under condition of pH 7.0, temperature 30 ℃, the initial concentration of U (VI) 20 mg/L and ZVI addition 1.0 g/L, the reduction rate of U(VI) reaches 96.9% by *** reduction for 24 h. The reduction rate of U(VI) is improved with increasing amount of ZVI in the range of 0.5-2.0 g/L, and the reduction rate of U(VI) is positive correlated with its initial concentration in the range of 5.0-50.0 mg/L. Fe3O4, Fe2O3 and ZVI are effective accelerators for bioreduction of U(VI), and soluble Fe(III) can remarkably inhibit U(VI) bioreduction. The other coexistence ions, such as Cu2+, Ca2+, Mn2+ and NO3-, show a remarkable negative effect on U(VI) reduction, and among these ions, the negative effect of Cu2+ is the greatest and that of Ca2+ is the second. SO42- has less influence on U(VI) bioreduction. XPS results indicate the deposition of U element are in forms of U(VI) and U(IV) on iron filings surface, and the reduction product U(IV) is most likely to be in the form of UO2. ©, 2015, Central South University of technology. All right reserved.

关键词： Bacteria

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Application of gray relational analysis to the experimental design on adsorption of U(VI) by Pseudomonas aeruginosa loaded by nano-Fe3O4

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Biotechnology: An Indian Journal 2014年第6期10卷 1640-1643页

作者： Rong, Li-Shan Xie, Shui-Bo Zeng, Tao-Tao Yang, Rui-Li Wu, Wei-Lin Hunan Provincial Key Laboratory of Pollution Control and Resources Technology University of South China Hengyang China Key Discipline Lab. for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy University of South China Hengyang China Hunan Sheng Long Project Management Ltd Zhuzhou China

This paper determines the main influence factors on the experiment of adsorption of U(VI) by Pseudomonas aeruginosa loaded by nano- Fe3O4 (NFPA) through gray correlation analysis, based on the experimental design on adsorption of U(VI) by Pseudomonas aeruginosa loaded by nano-Fe3O4. Results show that the method is simple, objective, reliable, and providing a scientific basis for experimental design to treatment the wastewater containing U(VI).

关键词： Magnetite

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Study of U(VI) removal by sulfate reducing bacteria granular sludge under micro-aerobic condition

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Yuanzineng Kexue Jishu/Atomic Energy Science and technology 2015年第1期49卷 26-33页

作者： Xie, Shui-Bo Ma, Hua-Long Tang, Zhen-Ping Ling, Hui Zeng, Tao-Tao Chen, Sheng Wang, Jin-Song Hunan Provincial Key Laboratory of Pollution Control and Resources Technology University of South China Hengyang421001 China Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy University of South China Hengyang421001 China

The bioremediation behavior and mechanism of sulfate reducing bacteria (SRB) granular sludge which was artificially domesticated were studied. U(VI) removal by SRB granular sludge was investigated under micro-aerobic condition with the concentration of oxygen 0.6-1.0 mg/L, then the removal mechanism was analyzed with FTIR, SEM and EDS, and finally the deposit morphology of uranium was studied with the sequential extraction procedure. The results show that U(VI) removal by SRB granular sludge mainly occurs following a 2-step process which is initial adsorption(the first 30 min) and microbial reduction process. The ultimate removal rate of U(VI) reaches 98.89%. Analyses of FTIR, SEM and EDS indicate that groups-amide group, carboxyl, hydroxyl and phosphoric acid group coming from the surface of SRB granular sludge are involved in uranium deposition. Ion exchange happens between Na+ or Mg2+ and UO22+. Finally, U(VI) mainly deposites on granular sludge in the form of residual state that has poor bioavailability, bad migratory aptitude and less secondary pollution. ©, 2015, Atomic Energy Press. All right reserved.

关键词： Ion exchange

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Influence factors and stability of U (VI) removal by sulfate reducing bacteria granular sludge

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Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals 2015年第6期25卷 1713-1720页

作者： Xie, Shui-Bo Chen, Sheng Ma, Hua-Long Tang, Zhen-Ping Zeng, Tao-Tao Ling, Hui Wu, Yu-Qi Hunan Provincial Key Laboratory of Pollution Control and Resources Technology University of South China Hengyang421001 China Key Discipline Laboratory of National Defence for Biotechnology in Uranium Mining and Hydrometallurgy University of South China Hengyang421001 China

The effect of initial U (VI) concentration, COD, SO42-, Cu2+ and Fe0 on U (VI) removal by sulfate reducing bacteria granular sludge (SRBGS) for U (VI) removal was investigated. The removal stability was analyzed, then the deposit valence of uranium was studied. The results show that U (VI) reduction prefers a higher COD and SO42- concentration at the range of 300-1500 mg/L. U (VI) removal is accelerated with increasing COD concentration and can be promoted when SO42- concentration is less than 1500 mg/L. The existing of Cu2+ do not affect U (VI) removal appreciably when its concentration is less than 100 mg/L, but U (VI) removal can not be inhibited completely while its concentration reaches 200 mg/L. Adding iron greatly improves U (VI) removal rate, U (VI) removal rate reaches 100% within 20 h. The SRBGS sludge can be long-term used, and the optimal hydraulic stay time (HRT) is 12.5 h. U (VI) can be reoxidized by NO3-. XPS analysis results indicate that the deposition of U element on SRBGS in main valence of U (IV). ©, 2015, Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals. All right reserved.

关键词： Bacteria

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An Integrated Socio-Economic and Ecological Framework for Evaluating the Societal Costs and Benefits of Fishing Activities in the Pearl River Delta

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Open Journal of Marine Science 2015年第4期5卷 477-497页

作者： Ying Wang Haoran Pan Shiyu Li Pierre Failler Zhejiang Provincial Key Research Institute of Philosophy and Social Sciences for Ecological Civilization School of Economics and Management Zhejiang Sci-Tech University Hangzhou China Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology School of Environmental Science and Engineering Sun Yat-sen University Guangzhou China School of Economics and Business Administration Beijing Normal University Beijing China Centre for the Economics and Management of Aquatic Resources (CEMARE) University of Portsmouth Portsmouth UK

This paper puts forward a model of Pearl River Delta (PRD) fishery in the South China Sea (SCS) that integrates the ecological, social and economic costs and benefits of fisheries activities in a multidisciplinary framework. In particular, an integrated ECOST model is composed of links between an ecological model constructed by Ecopath with Ecosim (EwE) software and a region Social Accounting Matrix (SAM). Then the costs and benefits of five fishing methods are compared from economic, ecological and social three dimensions base on the ECOST model. The potential effects of fishing effort reduction on fishing communication are explored by a series of dynamic simulations for a 10-year period. key results from prediction (2005-2015) and policy simulations illustrate that fisheries of PRE are geared toward short-term economic profits at the expense of ecological gains and the whole group of societal benefits associated with fishing. However, the status quo can be improved to better levels by reducing fishing efforts.

关键词： Integrated ECOST Model Ecopath with Ecosim Costs and Benefits Analysis Fishing Activities

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