检索结果-内蒙古大学图书馆

AI-driven multi-omics integration for multi-scale predictive modeling of genotype-environment-phenotype relationships

引用

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 2025年 27卷 265-277页

作者： Wu, You Xie, Lei CUNY Grad Ctr PhD Program Comp Sci New York NY 10016 USA CUNY Grad Ctr PhD Program Biol & Biochem New York NY USA CUNY Hunter Coll Dept Comp Sci New York NY USA Cornell Univ Weill Cornell Med Helen & Robert Appel Alzheimers Dis Res Inst Feil Family Brain & Mind Res Inst New York NY USA

Despite the wealth of single-cell multi-omics data, it remains challenging to predict the consequences of novel genetic and chemical perturbations in the human body. It requires knowledge of molecular interactions at all biological levels, encompassing disease models and humans. Current machine learning methods primarily establish statistical correlations between genotypes and phenotypes but struggle to identify physiologically significant causal factors, limiting their predictive power. Key challenges in predictive modeling include scarcity of labeled data, generalization across different domains, and disentangling causation from correlation. In light of recent advances in multi-omics data integration, we propose a new artificial intelligence (AI)-powered biologyinspired multi-scale modeling framework to tackle these issues. This framework will integrate multi-omics data across biological levels, organism hierarchies, and species to predict genotype-environment-phenotype relationships under various conditions. AI models inspired by biology may identify novel molecular targets, biomarkers, pharmaceutical agents, and personalized medicines for presently unmet medical needs.

关键词： Machine learning Deep learning Single cell Omics data Drug discovery Precision medicine Complex disease

来源：评论

学校读者我要写书评

暂无评论

Optimizing adhesion protocols between 3D-printed ceramic-containing composite and resin cement

引用

INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES 2025年 140卷

作者： Souza, Luiza Freitas Brum Teixeira, Ketlin Fagundes Cadore-Rodrigues, Ana Carolina Binotto, Felipe Somavilla Daudt, Natalia de Freitas Valandro, Luiz Felipe Moraes, Rafael R. Ozcan, Mutlu Pereira, Gabriel Kalil Rocha Fed Univ Santa Maria UFSM Fac Dent MSciD & PhD Postgrad Program Oral Sci Santa Maria RS Brazil Fed Univ Santa Maria UFSM Fac Mech Engn MSciEng & PhD Postgrad Program Mech Engn Santa Maria RS Brazil Univ Fed Pelotas Sch Dent Pelotas RS Brazil Univ Zurich Ctr Dent Med Clin Masticatory Disorders & Dent Biomat Zurich Switzerland

This study evaluated the microshear bond strength (mu SBS) of resin cement to a 3D-printed ceramic-containing resin composite (PriZma 3D Bio Crown, Makertech) subjected to different adhesive protocols (surface pretreatments and adhesive systems), in both baseline and aging conditions. For this, 3D-printed resin composite slices were fabricated and divided into groups based on adhesive protocols, considering three surface pretreatments: alumina grit-blasting (AlOx), 37 % phosphoric acid (PA), and a combination of both (AlOx + PA);and two adhesive systems: conventional (Adper Single Bond 2, 3M) and universal (Single Bond Universal, 3M). The resin cement (RelyX Ultimate, 3M) cylinder specimens (n = 18) underwent mu SBS testing before and after thermocycling (12,000 cycles, 5-55 degrees C). Surface roughness (R-a and R-z), SEM topography, and failure modes were analyzed. Statistical analysis included One- and Two-Way ANOVA with Tukey tests for roughness and mu SBS, respectively, and paired t-test to compare baseline and aging conditions considering the same adhesive protocol (alpha = 0.05). AlOx + PA with the universal adhesive demonstrated the highest bond strength in both baseline and aged conditions, with a minimal reduction after aging (11.68 %, p = 0.294). The PA-conventional adhesive group had the greatest reduction post-aging (50.32 %, p < 0.001). After aging, AlOx and PA with universal adhesive showed intermediate bond strengths, similar to AlOx + PA-universal, while the use of conventional adhesive resulted in lower bonding performance. In Baseline, AlOx + PA-universal exhibited the highest Weibull modulus, while AlOx + PA-conventional had the lowest. After aging, AlOx + PA-universal remained the highest, differing from the other groups but similar to AlOx + PA-conventional, whereas PA-universal had the lowest modulus. The other groups showed intermediate values without significant differences. The combination of alumina grit-blasting and phosphoric acid with a

关键词： 3D printed dental resin Resin composite Dental materials Microshear bond strength Surface treatment Aging

来源：评论

学校读者我要写书评

暂无评论

Encapsulation of Gold-Based Anticancer Agents in Protease-Degradable Peptide Nanofilaments Enhances Their Potency

引用

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2023年第1期145卷 234-246页

作者： Marciano, Yaron del Solar, Virginia Nayeem, Nazia Dave, Dhwanit Son, Jiye Contel, Maria Ulijn, Rein, V CUNY Adv Sci Res Ctr Nanosci Initiat Grad Ctr New York NY 10031 USA CUNY Brooklyn Coll Dept Chem Brooklyn NY 11210 USA Guys Hosp Cell & Gene Therapy Catapult 12th Floor Tower Wing London SE1 9RT England CUNY PhD Program Chem Grad Ctr New York NY 10016 USA CUNY Hunter Coll Dept Chem New York NY 10065 USA CUNY PhD Program Biochem PhD Program Chem Grad Ctr New York NY 10016 USA CUNY PhD Program Biochem Grad Ctr New York NY 10016 USA

We investigated the use of amphiphilic, protease-cleavable peptides as encapsulation moieties for hydrophobic metallodrugs, in order to enhance their bioavailability and consequent activity. Two hydrophobic, gold-containing anticancer agents varying in aromatic ligand distribution (Au(I)-N-heterocyclic carbene compounds 1 and 2) were investigated. These were encapsulated into amphiphilic decapeptides that form soluble filamentous structures with hydrophobic cores, varying supramolecular packing arrangements and surface charge. Peptide sequence strongly dictates the supramolecular packing within the aromatic core, which in turn dictates drug loading. Anionic peptide filaments can effectively load 1, and to a lesser extent 2, while their cationic counterparts could not, collectively demonstrating that loading efficiency is dictated by both aromatic and electrostatic (mis)matching between drug and peptide. Peptide nanofilaments were nontoxic to cancerous and noncancerous cells. By contrast, those loaded with 1 and 2 displayed enhanced cytotoxicity in comparison to 1 and 2 alone, when exposed to Caki-1 and MDA-MB-231 cancerous cell lines, while no cytotoxicity was observed in noncancerous lung fibroblasts, IMR-90. We propose that the enhanced in vitro activity results from the enhanced proteolytic activity in the vicinity of the cancer cells, thereby breaking the filaments into drug-bound peptide fragments that are taken up by these cells, resulting in enhanced cytotoxicity toward cancer cells.

关键词： Cells Encapsulation Filaments Gold Peptides and proteins

来源：评论

学校读者我要写书评

暂无评论

Unleashing viral mimicry: A combinatorial strategy to enhance the efficacy of PARP7 inhibitors

引用

BIOESSAYS 2025年第2期47卷 e2400087页

作者： Manetsch, Patrick Hottiger, Michael O. Univ Zurich Dept Mol Mech Dis Zurich Switzerland Univ Zurich Life Sci Zurich Grad Sch Mol Life Sci PhD Program Zurich Switzerland

Cancer cells exploit mechanisms to evade immune detection triggered by aberrant self-nucleic acids (NA). PARP7, a key player in this immune evasion strategy, has emerged as a potential target for cancer therapy. PARP7 inhibitors reactivate NA sensing, resulting in type I interferon (IFN) signaling, programmed cell death, anti-tumor immunity, and tumor regression. Cancer cells with elevated IFN-stimulated gene (ISG) scores, representing a viral mimicry-primed state, are particularly sensitive to PARP7 inhibition. This review focuses on the endogenous sources of NA in cancer and the potential to exploit elevated aberrant self-NA in cancer therapy. We describe strategies to increase cytoplamic NA levels, including targeting epigenetic control, DNA damage response, and mitochondrial function. We also discuss targeting RNA processing pathways, such as splicing and RNA editing, to enhance the immunostimulatory potential of existing NA. Combining PARP7 inhibitors with NA elevating strategies may improve cancer immunotherapy, especially for tumors with high ISG scores.

关键词： anti-tumor immunity cancer therapy FRA1 PARP7 programmed cell death viral mimicry

来源：评论

学校读者我要写书评

暂无评论

Optical Aptamer-Based Cytokine Nanosensor Detects Macrophage Activation by Bacterial Toxins

引用

ACS SENSORS 2024年第7期9卷 3697-3706页

作者： Ryan, Amelia K. Rahman, Syeda Williams, Ryan M. CUNY City Coll New York Dept Biomed Engn New York NY 10031 USA CUNY Grad Ctr PhD Program Chem New York NY 10016 USA

Overactive or dysregulated cytokine expression is a hallmark of many acute and chronic inflammatory diseases. This is true for acute or chronic infections, neurodegenerative diseases, autoimmune diseases, cardiovascular diseases, cancer, and others. Cytokines such as interleukin-6 (IL-6) are known therapeutic targets and biomarkers for such inflammatory diseases. Platforms for cytokine detection are, therefore, desirable tools for both research and clinical applications. Single-walled carbon nanotubes (SWCNT) are versatile nanomaterials with near-infrared fluorescence that can serve as transducers for optical sensors. When functionalized with an analyte-specific recognition element, SWCNT emission may become sensitive and selective toward the desired target. SWCNT-aptamer sensors are easily assembled, inexpensive, and biocompatible. In this work, we introduced a nanosensor design based on SWCNT and a DNA aptamer specific to IL-6. We first evaluated several SWCNT-aptamer constructs based on this simple direct complexation method, wherein the aptamer both solubilizes the SWCNT and confers sensitivity to IL-6. The sensor limit of detection, 105 ng/mL, lies in the relevant range for pathological IL-6 levels. Upon investigation of sensor kinetics, we found rapid response within seconds of antigen addition which continued over the course of 3 h. We found that this sensor construct is stable and the aptamer is not displaced from the nanotube surface during IL-6 detection. Finally, we investigated the ability of this sensor construct to detect macrophage activation caused by bacterial lipopolysaccharides (LPS) in an in vitro model of disease, finding rapid and sensitive detection of macrophage-expressed IL-6. We are confident that further development of this sensor will have novel implications for diagnosis of acute and chronic inflammatory diseases, in addition to contributing to the understanding of the role of cytokines in these diseases.

关键词： IL-6 DNA aptamer inflammation nanocarbon SWCNT fluorescence

来源：评论

学校读者我要写书评

暂无评论

The increasing authorship trend in neuroscience: A scientometric analysis across 11 countries

引用

IBRO NEUROSCIENCE REPORTS 2024年 17卷 52-57页

作者： Paul, Ann Segreti, Mariella Pani, Pierpaolo Brunamonti, Emiliano Genovesio, Aldo Sapienza Univ Dept Physiol & Pharmacol Rome Italy Sapienza Univ Behav Neurosci PhD Program Rome Italy

Previous studies have demonstrated an increasing trend of the number of authors across various fields over the years. This trend has been attributed to the necessity for larger collaborations and, at times, to ethical issues regarding authorship attribution. Our study focuses on the evolution of authorship trends in the field of Neuroscience. We conducted our analysis based on a dataset containing 580,782 neuroscience publications produced from 2000 to 2022, focusing on the publications within the Group of ten (G10) countries. Using a matrix-based methodology, we extracted and analyzed the average number of authors per country. Our findings reveal a consistent rise in authorship across all G10 countries over the past two decades. Italy emerged with the highest average number of authors, while France stood out for experiencing the most significant increase, particularly in the last decade. The countries with the lowest number of authors per publication were the USA, UK and Canada. Differences between countries could result from variations in the size of collaboration between researchers in different countries. Additionally, these differences may depend on utilitarian considerations aimed at receiving higher scores in the individual evaluation of their own work. We propose that a normalization procedure for the number of authors should be implemented to ensure a fair evaluation of researchers.

关键词： Neuroscience Authorship G10 countries

来源：评论

学校读者我要写书评

暂无评论

A preliminary design of a 3D maritime gamified mentoring platform to support tanker pre-vetting inspection training: 'Maritime Gamentor'

引用

SHIPS AND OFFSHORE STRUCTURES 2023年第12期18卷 1627-1637页

作者： Gurbuz, Suleyman Cihan Celik, Metin Istanbul Tech Univ Dept Marine Transportat Engn PhD Program Istanbul Turkey Istanbul Tech Univ Dept Basic Sci Istanbul Turkey

Pre-vetting inspection, enhanced with onsite training, is a critical process to improve safety and environmental compliance onboard tanker ships. As a part of pre-vetting services, this study develops a maritime gamified mentoring platform, namely Maritime Gamentor, to manage crew upskilling in tanker vetting requirements. In this stage, the study considers the recently updated requirements of the Ship Inspection Report (SIRE 2.0) programme of Oil Companies International Marine Forum (OCIMF) to conceptualise a holistic serious game-based training solution. Then, a step-by-step approach to the preliminary design, development and prototyping of maritime serious games is provided in detail. Particular to the SIRE 2.0 Clause# 5.1.4, the study conducts a case study on Maritime Gamentor Module-01 illustrated with a task-based risk assessment (TBRA) on enclosed space entry operation. Consequently, the Maritime Gamentor is conceptualised as a powerful training and mentorship platform for global shipping companies.

关键词： Serious games maritime mentoring risk assessment tanker vetting safety training

来源：评论

学校读者我要写书评

暂无评论

Sub-5-min RP-UHPLC-TIMS for high-throughput untargeted lipidomics and its application to multiple matrices

引用

ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2024年第4期416卷 959-970页

作者： Merciai, Fabrizio Basilicata, Manuela Giovanna La Gioia, Danila Salviati, Emanuela Caponigro, Vicky Ciaglia, Tania Musella, Simona Crescenzi, Carlo Sommella, Eduardo Campiglia, Pietro Univ Salerno Dept Pharm Via Giovanni Paolo II 132 I-84084 Fisciano SA Italy Univ Salerno PhD Program Drug Discovery & Dev Fisciano SA Italy

Untargeted lipidomics, with its ability to take a snapshot of the lipidome landscape, is an important tool to highlight lipid changes in pathology or drug treatment models. One of the shortcomings of most untargeted lipidomics based on UHPLC-HRMS is the low throughput, which is not compatible with large-scale screening. In this contribution, we evaluate the application of a sub-5-min high-throughput four-dimensional trapped ion mobility mass spectrometry (HT-4D-TIMS) platform for the fast profiling of multiple complex biological matrices. Human AC-16 cells and mouse brain, liver, sclera, and feces were used as samples. By using a fast 4-min RP gradient, the implementation of TIMS allows us to differentiate coeluting isomeric and isobaric lipids, with correct precursor ion isolation, avoiding co-fragmentation and chimeric MS/MS spectra. Globally, the HT-4D-TIMS allowed us to annotate 1910 different lipid species, 1308 at the molecular level and 602 at the sum composition level, covering 58 lipid subclasses, together with quantitation capability covering more than three orders of magnitude. Notably, TIMS values were highly comparable with respect to longer LC gradients (CV% = 0.39%). These results highlight how HT-4D-TIMS-based untargeted lipidomics possess high coverage and accuracy, halving the analysis time with respect to conventional UHPLC methods, and can be used for fast and accurate untargeted analysis of complex matrices to rapidly evaluate changes of lipid metabolism in disease models or drug discovery campaigns.

关键词： High-throughput Trapped ion mobility PASEF Untargeted lipidomics

来源：评论

学校读者我要写书评

暂无评论

Strategies of positron emission tomography (PET) tracer development for imaging of tau and α-synuclein in neurodegenerative disorders

引用

RSC MEDICINAL CHEMISTRY 2025年第2期16卷 605-639页

作者： Mekala, Shekar Wu, You Li, Yue-Ming Mem Sloan Kettering Canc Ctr Chem Biol Program 1275 York Ave New York NY 10065 USA Mem Sloan Kettering Canc Ctr Triinst PhD Program Chem Biol New York NY 10065 USA

Alzheimer's disease (AD) is a progressive neurodegenerative disorder, characterized by the presence of extracellular amyloid plaques consisting of beta-amyloid peptides (A beta) and intracellular neurofibrillary tangles (NFTs) composed of hyperphosphorylated tau (pTau) protein in the brain. Genetic and animal studies strongly indicate that A beta, tau and neuroinflammation play important roles in the pathogenesis of AD. Several staging models showed that NFTs correlated well with the disease progression. Positron emission tomography (PET) imaging has become a widely used non-invasive technique to image NFTs for early diagnosis of AD. Despite the remarkable progress made over the past few years, tau PET imaging is still challenging due to the nature of tau pathology and the technical aspects of PET imaging. Tau pathology often coexists with other proteinopathies, such as A beta plaques and alpha-synuclein aggregates. Distinguishing tau-specific signals from other overlapping pathologies is difficult, especially in the context of AD, where multiple protein aggregates are present, as well as the spectrum of different tau isoforms (3R and 4R) and conformations. Moreover, tracers should ideally have optimal pharmacokinetic properties to penetrate the blood-brain barrier (BBB) while maintaining specificity, low toxicity, low non-specific binding, rapid uptake and clearance from the brain, and formation of no radiolabeled metabolites in the brain. On the other hand, Parkinson's disease (PD) is a progressive neurodegenerative movement disorder characterized by the abnormal accumulations of alpha-synuclein in neurons. Heterogeneity and the unclear pathogenesis of PD hinder early and accurate diagnosis of the disease for therapeutic development in clinical use. In this review, while referring to existing reviews, we focus on the design strategies and current progress in tau (NFTs) targeting new PET tracers for AD;evolution of non-AD tau targeting PET tracers for applications

关键词：

来源：评论

学校读者我要写书评

暂无评论

miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells

引用

GENOMICS 2024年第2期116卷 110820页

作者： van Gelderen, Tosca A. Ribas, Laia Consejo Super Invest Cient ICM CSIC Inst Ciencies Mar Barcelona Spain Autonomous Univ Barcelona UAB PhD Program Genet Bellaterra 08193 Spain

microRNA-210 (miRNA), a well-documented miRNA, has been implicated in a myriad of biological processes, including responses to hypoxia, angiogenesis, cell proliferation, and male infertility in humans. However, a comprehensive understanding of its functions in fish requires further investigation. This study pursued to elucidate the downstream effect of dre-miR-210-5p on primary ovarian cell culture in zebrafish (Danio rerio), an animal model. A protocol was settled down by incubations with either an miR-210 mimic or a scrambled miRNA in the isolated ovaries. RNA-sequencing analysis identified similar to 6000 differentially expressed target genes revealing that downregulated genes were associated with reproduction-related pathways while immune-related pathways displayed an upregulated pattern. To identify molecular markers, predicted target genes were classified into reproduction and immune cell types. These findings underscore the existence of a profound interplay between the reproductive and immune systems, with miR-210 emerging as a pivotal player in orchestrating transcriptomic alterations within fish ovaries.

关键词： Epigenetics Cell culture Reproduction Infection Gamete Protocol Marker

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：