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Large-scale parametric study of electroporation in cancer cells to construct phase diagrams using micro cellarray chips

Large-scale parametric study of electroporation in cancer ce...

引用

9th International Conference on Miniaturized systems for Chemistry and Life Sciences, MicroTAS 2005

作者： He, Huiqi Chang, Donald C. Lee, Yi-Kuen Bioengineering Graduate Program Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Dept. of Biology Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Dept. of Mechanical Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)0974361119

In this study, a new micro electroporation cell chip was fabricated by MEMS technology and tested by using cervical cancer (HeLa) cells. Large statistical data of the critical voltage and duration time were determined to construct the "phase diagram" which delineating the boundary for effective electroporation and electric cell lysis at the single-cell level. Copyright © 2005 by the Transducer Research Foundation, Inc.

关键词： Cancer cells

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Electrical conductivity of lambda DNA-Pd wire

Electrical conductivity of lambda DNA-Pd wire

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Optomechatronic Micro/Nano Devices and Components

作者： Hosogi, Maho Hashiguchi, Gen Haga, Masa-Aki Yonezawa, Tetsu Kakushima, Kuniyuki Fujita, Hiroyuki Department of Intelligent Mechanical Systems Engineering Faculty of Engineering Kagawa University 2217-20 Hayashi-cho Takamatsu 761-0396 Japan Department of Applied Chemistry Faculty of Science and Engineering Chuo University 1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan Department of Chemistry School of Science University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Interdisciplinary Graduate School of Science Engineering Department of Electronics and Applied Physics Tokyo Institute of Technology 4259 Nagatsuta-cho Yokohama Kanagawa 226-8503 Japan Institute of Industrial Science University of Tokyo 4-6-1 Komaba Meguro-ku Tokyo 153-8505 Japan

Recently DNA molecules have been focused on as electronic elements in the field of nanometer-scale electronics. One of the fundamental issues in this research area is the development of methods to measure accurately an electrical conductivity of DNA wire. The DNA tweezers we have recently developed have a great advantage of measuring the electrical current passing through DNA wire. In this paper, we investigated the electric conduction of lambda DNA molecules covered with Pd colloids using micromachined DNA tweezers that has a pair of opposing probes for retrieving DNA molecules. The molecules were retrieved from a solution containing lambda DNA by applying RF power between the probes in the solution. The retrieved molecules were then soaked in a colloidal solution containing cationic Pd particles, which results in a DNA-Pd wire bridged between the tweezer probes. Current-voltage curves for the DNA-Pd wire can be measured between the DNA tweezers probes, and the resistivity of the DNA-Pd wire was approximately 74 Qcm. We found through an observation by a scanning transmission electron microscope (STEM) that the surface of the wire was covered by Pd particles closely. Wejalso measured the piezoresistance through a change in the distance between Pd particles on a DNA-Pd wire using the DNA tweezers.

关键词： Electric wire

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Online dense local 3D world reconstruction from stereo image sequences

Online dense local 3D world reconstruction from stereo image...

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2005 IEEE/RSJ International Conference on Intelligent Robots and systems

作者： S. Kagami Y. Takaoka Y. Kida K. Nishiwaki T. Kanade CREST Program Japan Science and Technology Agency Japan Graduate School of Information Science Nara Institute of Science and Technology Japan Digital Human Research Center National Institute for Advanced Industrial Science and Technology Japan Department of Mechanical Engineering Tokyo University of Science Japan Robotics Institute Carnegie Mellon University USA

This paper describes an online 3D reconstruction system from stereo image sequences to obtain a dense local world model for robot navigation. The proposed method consists of three components: 1) stereo depth map calculation, 2) correspondence calculation in time sequential images by tracking raw image features, 3) 6DOF camera motion estimation by RANSAC and integrate depth map into 3D reconstructed model. We examined and evaluated our method in a motion capture environment for comparison. Finally experimental results of a humanoid robot H7 are denoted.

关键词： Image reconstruction Stereo image processing Image sequences Cameras Stereo vision Humanoid robots Surface reconstruction Robot vision systems Mobile robots Tracking

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Behavior model of humanoid robots based on operant conditioning

Behavior model of humanoid robots based on operant condition...

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IEEE-RAS International Conference on Humanoid Robots

作者： K. Itoh H. Miwa M. Matsumoto M. Zecca H. Takanobu S. Roccella M.C. Carrozza P. Dario A. Takanishi Department of Mechanical Engineering Waseda University Tokyo Japan Institute for Biomedical Engineering ASMew Waseda University Tokyo Japan Digital Human Research Center National Institute for Advanced Industrial Science and Technology Tokyo Japan Graduate School of Science and Engineering Waseda University Tokyo Japan Scuola Superiore Sant'Anna ARTS Laboratory Pontedera Italy Humanoid Robotics Institute (HRI) Waseda University Tokyo Japan Department of Mechanical Systems Engineering Kogakuin University Tokyo Japan Advanced Research Institute for Science and Engineering Waseda University Shinjuku Tokyo Japan

Personal robots, which are expected to become popular in the near future, are required to be active in work and community life alongside humans. Therefore, we have been developing new mechanisms and functions in order to realize natural bilateral interaction by expressing emotions, behaviors and personality in a human-like manner. We have proposed a mental model with emotion, mood, personality and needs. However, the robot behavior was very simple since the robot shows just a single kind of behavior in response to a robot mental state. In this paper, we present a new behavior model for humanoid robots based on operant conditioning, which is a well-known psychological behavior model. We implemented this new behavior model into the emotion expression humanoid robot WE-4RII (Waseda Eye No.4 Refined II), developed in 2004. Through the experimental evaluations, we confirmed that the robot with the new behavior model could autonomously select suitable behavior for the situation within a predefined behavior list

关键词： Humanoid robots Robot kinematics Humans Service robots Biomedical engineering Cognitive science Psychology Orbital robotics Mood Robotic assembly

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Object detection based on radial reach filter under the change of background

Object detection based on radial reach filter under the chan...

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Optomechatronic Machine Vision

作者： Wajima, N. Takahashi, S. Ito, M. Satoh, Y. Kaneko, S. Department of Intelligent Mechanical Systems Engineering Kagawa University 2217-20 Hayashi-Cho Takamatsu-City Kagawa 761-0396 Japan Hitachi Research Laboratory Hitachi Ltd. 7-1-1 Oomika-Cho Hitachi-City Ibaragi 319-1292 Japan National Institute of Advanced Industrial Science and Technology 1-1-1 Umezono Tukuba-City Ibaragi 305-8568 Japan Graduate School of Information Science and Technology Hokkaido University Kita14 Nishi9 Sapporo-City Hokkaido 060-0814 Japan

In this paper, we propose a new method of object detection. In the past, there are various methods of object detection. Especially, the method of the background subtraction has the effectiveness. However, the methods based on brightness differences are easily influenced by change in lighting condition. In this paper, we use Radial Reach Filter (RRF). RRF is called as the effective method of the change in lighting conditions. However, RRF is not considered change that caused by moving objects on the background image. Then, we propose the new method of object detection that considered motion of the moving objects on the background image. And. we verify the effectiveness by the experiments using a time series image.

关键词： Object recognition

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Economic impact for integrating constructivism, project-based learning and practice into high quality professional graduate education for engineers in industry to enhance corporate advantage and U.S. competitiveness in the global economy

Economic impact for integrating constructivism, project-base...

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2005 ASEE Annual Conference and Exposition: The Changing Landscape of engineering and Technology Education in a Global World

作者： Snellenberger, J.M. Quick, D.H. Tidwell, J.P. O'Brien, J. Davis, T.I. McHenry, A.L. Bardo, J.W. Dunlap, D.D. Deloatch, E.M. Lee, P.Y. Palmer, H.J. Tricamo, S.J. Depew, D.R. Bertoline, G.R. Dyrenfurth, M.J. Keating, D.A. Stanford, T.G. Rolls-Royce Corporation United States Boeing Company United States Hewlett-Packard United States Raytheon Missile Systems United States Arizona State University East United States Western Carolina University United States Morgan State University United States California Polytechnic State University United States Rochester Institute of Technology United States New Jersey Institute of Technology United States Purdue University United States Rolls-Royce Corporation Indianapolis IN United States ASEE Corporate Members Council United States Allison Advanced Development Company Rolls-Royce Corporation Indianapolis IN United States Boeing Company Meza AN United States Hewlett-Packard Company Palo Alto CA United States Raytheon Missile Systems Tucson AZ United States College of Technology and Applied Sciences Arizona State University East United States Department of Engineering and Technology Western Carolina University United States ASEE Graduate Studies Division School of Engineering Morgan State University United States ASEE College of Engineering California State Polytechnic University United States Kate Gleason College of Engineering Rochester Institute of Technology United States Department of Industrial and Manufacturing Engineering College of Technology Purdue University United States Discovery Resources Envision Center Department of Computer Graphics College of Technology Purdue University United States Department of Graduate Studies College of Technology Purdue University United States Department of Mechanical Engineering University of South Carolina United States Department of Chemical Engineering University of South Carolina United States

This is the fourth of four papers prepared for a special panel session of the National Collaborative Task Force on engineering graduate Education Reform focusing on new educational approaches and processes that better meet the development needs of the U.S. engineering workforce in industry to enhance global competitiveness. Further graduate development of the U.S. engineering workforce in industry is critical to the continuous improvement, invention, development, and innovation of new technology which is the engine for U.S. economic prosperity and competitiveness. This paper focuses on a unique model for workforce development that represents a significant advancement in professional graduate education extending through the professional masters, professional doctorate and fellow levels of engineering practice. This advancement in professional graduate education forms a new partnership for university-industry engagement for U.S. engineering workforce development that completes a missing piece of the process to better enable U.S. technological innovation which is long overdue. The impact of project-based learning on industrial innovation is evidenced. This model is designed specifically to support the on-site engineering process for continuous improvement and innovation in industry. It purposefully integrates postgraduate professional education for industry's employed engineers with on-site technology development projects that are chosen to be directly relevant to industry's continuous innovation needs and high-end engineering projects. The returns for enhanced corporate advantage generated through this unique model are measured as a matrix of increasing complexity of economic worth of on-site projects and of increasing human proficiency gained for leadership of technological innovation.

关键词： Learning systems

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Sequencing with limited flexibility on moving assembly lines

Sequencing with limited flexibility on moving assembly lines

引用

IIE Annual Conference and Exhibition 2004

作者： Lahmar, Maher Benjaafar, Saif Department of Industrial Engineering University of Houston Houston TX 77204-4008 Graduate Program in Industrial Engineering Department of Mechanical Engineering University of Minnesota Minneapolis MN 55455-0111

We consider the problem of resequencing a set of prearranged jobs when there is limited resequencing flexibility and sequence dependent changeover costs. Resequencing flexibility is limited by how far forward or backward a job can shift in the sequence relative to its original position. We show how the problem can be solved using dynamic programming in time polynomial in the number of jobs. We also provide numerical results to support managerial insights regarding the value of flexibility. We show that our procedure can be extended to solve a variety of sequencing problems on moving assembly lines.

关键词： Dynamic programming

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Strategies to attract information technology students: An extrapolation of worker experiences

Strategies to attract information technology students: An ex...

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ASEE 2004 Annual Conference and Exposition, "engineering Researchs New Heights"

作者： Cullinane, Thomas P. Perry, Ronald F. Yanmaz, Baris Department of Mechanical Engineering Northeastern University Boston MA Graduate Information Systems Program Northeastern University Boston MA Northeastern University Boston MA

The predicted dramatic increase in demand for IT workers in the next decade suggests an opportunity for IT educators to establish plans to ramp up to meet these needs. The purpose of this paper is to help educators understand factors that are important to IT workers and that can be influenced by the educational process. The work reported here is part of an NSF grant that explored current IT worker experiences and motivations. Data for the study was collected using a forty-four-item questionnaire sent to currently employed IT workers in the Boston area. Stepwise multiple linear regression was used to relate certain outcome variables to attributes and opinions of the respondents as represented by question item responses. Relationships have been discovered that will help educators craft academic programs and identify potentially successful students in IT. For example, the analysis indicates questionnaire items associated with the challenge presented by an IT profession and family educational backgrounds in technical areas are important outcome predictors, while gender and age are less important.

关键词： Strategic planning

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Mixed 2/∞ control for uncertain systems under pole placement constraints using Genetic Algorithms and LMIs

Mixed 2/∞ control for uncertain systems under pole placemen...

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Proceedings of the 2004 IEEE International Symposium on Intelligent Control - 2004 ISIC

作者： Neumann, Donald De Araújo, Humberto X. Industrial and Systems Engineering Graduate Program - PPGEPS Pontifical Catholic University of Paraná PUC-PR Brazil PPGEPS PUC-PR Brazil

This paper is concerned with the mixed 2/ ∞ control problem combined with robust pole placement in linear matrix inequality (LMI) regions. Continuous-time linear systems subject to polytopic parametric uncertainties are studied. Based on Genetic Algorithms (GAs) and LMIs, a hybrid algorithm is presented for numerical computation of a robust fixed-order, static or dynamic, output feedback controller. This approach does not require that all specifications are enforced by a single closed-loop Lyapunov function. This allows to reduce the conservatism of the usual existing methods. In addition, this approach can be used for synthesis for reduced or full order controllers. Examples borrowed from the, literature are discussed to validate this approach. © 2004 IEEE.

关键词： Uncertain systems

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Leveraging expertise in time of economic constraints

Leveraging expertise in time of economic constraints

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ASEE 2004 Annual Conference and Exposition, "engineering Researchs New Heights"

作者： Thongsamak, Sasima Scales, Glenda R. Peed, Cheryl Virginia Tech. Industrial Engineering Department Virginia Tech. Distance Learning and Computing College of Engineering Cmw. Graduate Engineering Program Instructional and Research Services College of Engineering

To address the need to improve the Commonwealth graduate engineering program (CGEP) at Virginia Tech, the college relied upon the expertise of students completing graduate theses and group projects in the industrial and systems engineering Department. This arrangement allowed graduate students to work on real problems as well as conduct applied research projects for an organization - the College of engineering. The students benefited from the experience and the college benefited from the result. CGEP is a coalition of five Virginia universities that deliver engineering graduate degree programs through distance learning. Three years ago this program was under review by the State Council of Higher Education for Virginia. Given that the CGEP director and administrators were new to this program, it was a high priority to establish metrics to determine the program's success. Another high priority was to search for expertise in the area of performance measurements. During the past three years, progress has been made in creating a continuous-process improvement plan for the program through the application of management systems methodologies. This paper discusses how graduate student expertise was used to assist with improving administrative aspects of the CGEP. It also describes the progression of projects and how data was analyzed and used to establish future direction. The concept described in this paper is traditional, yet the success of the methods used to improve the Commonwealth graduate engineering program offers a new way to apply management system methodologies.

关键词： engineering education

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