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arXiv 2021年

作者： Yang, Jijun Abdukerim, Abdusalam Chen, Wei Chen, Xun Chen, Yunhua Cheng, Chen Cui, Xiangyi Fan, Yingjie Fang, Deqing Fu, Changbo Fu, Mengting Geng, Lisheng Giboni, Karl Gu, Linhui Guo, Xuyuan Han, Ke He, Changda He, Shengming Huang, Di Huang, Yan Huo, Ran Huang, Yanlin Huang, Zhou Ji, Xiangdong Ju, Yonglin Li, Shuaijie Lin, Qing Liu, Huaxuan Liu, Jianglai Lu, Xiaoying Ma, Wenbo Ma, Yugang Mao, Yajun Meng, Yue Shaheed, Nasir Ni, Kaixiang Ning, Jinhua Ning, Xuyang Ren, Xiangxiang Shang, Changsong Shen, Guofang Si, Lin Tan, Andi Wang, Anqing Wang, Hongwei Wang, Meng Wang, QiuHong Wang, Siguang Wang, Wei Wang, Xiuli Wang, Zhou Wu, Mengmeng Wu, Shiyong Wu, Weihao Xia, Jingkai Xiao, Mengjiao Xiao, Xiang Xie, Pengwei Yan, Binbin Yang, Yong Yu, Chunxu Yu, Hai-Bo Yuan, Jumin Yuan, Ying Zeng, Xinning Zhang, Dan Zhang, Tao Zhao, Li Zheng, Qibin Zhou, Jifang Zhou, Ning Zhou, Xiaopeng School of Physics and Astronomy Shanghai Jiao Tong University MOE Key Laboratory for Particle Astrophysics and Cosmology Shanghai Key Laboratory for article Physics and Cosmology Shanghai200240 China Shanghai Jiao Tong University Sichuan Research Institude Chengdu610213 China Yalong River Hydropower Development Company Ltd. 288 Shuanglin Road Chengdu610051 China School of Physics Sun Yat-Sen University Gaungzhou510275 China Tsung-Dao Lee Institute Shanghai200240 China School of Physics Nankai University Tianjin300071 China Institute of Modern Physics Fudan University Shanghai200433 China School of Physics Peking University Beijing100871 China School of Physics Beihang University Beijing100191 China International Research Center for Nuclei and Particles in the Cosmos Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang University Beijing100191 China Shandong Institute of Advanced Technology Jinan250103 China School of Medical Instrument and Food Engineering University of Shanghai for Science and Technology Shanghai200093 China Department of Physics University of Maryland College ParkMD20742 United States School of Mechanical Engineering Shanghai Jiao Tong University Shanghai200240 China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Hefei230026 China Department of Modern Physics University of Science and Technology of China Hefei230026 China Key Laboratory of Particle Physics and Particle Irradiation of Ministry of Education Shandong University Jinan250100 China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary Scienc Shandong University Shandong Qingdao266237 China Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai201210 China Department of Physics and Astronomy University of California RiversideCA92507 United States Center of High Energy Physics Peking University Beijing100871 C

Self-interacting Dark Matter (SIDM) is a leading candidate proposed to solve discrepancies between predictions of the prevailing cold dark matter theory and observations of galaxies. Many SIDM models predict the existence of a light force carrier that mediate strong dark matter self-interactions. If the mediator couples to the standard model particles, it could produce characteristic signals in dark matter direct detection experiments. We report searches for SIDM models with a light mediator using the full dataset of the PandaX-II experiment, based on a total exposure of 132 tonne-days. No significant excess over background is found, and our likelihood analysis leads to a strong upper limit on the dark matter-nucleon coupling strength. We further combine the PandaX-II constraints and those from observations of the light element abundances in the early universe, and show that direct detection and cosmological probes can provide complementary constraints on dark matter models with a light mediator. © 2021, CC BY-NC-ND.

关键词： Dark Matter

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Spin-polarized GaAs surface studied by first-principles method with SO interaction for THz emission application

Spin-polarized GaAs surface studied by first-principles meth...

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International Conference on infrared, Millinmeter, and Terahertz Waves

作者： Mary Clare S. Escano Hideaki Kasai Masahiko Tani Research Center for Development of Far-Infrared Region University of Fukui Fukui Japan National Institute of Technology Akashi Hyogo Japan

Because of the broken inversion symmetry of GaAs, we studied the unreconstructed GaAs surface, using first principles method with spin-orbit (SO) interaction. We found that the highest-energy valence band is split, giving rise to spin-polarized electrons. The band originate mainly from the surface, leading to 2D spin-polarized electron spin source.

关键词： Gallium arsenide Magnetic semiconductors Two dimensional displays Topological insulators Mathematical model Magnetic confinement Atomic layer deposition

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Improved thermal model considering hydrate formation and deposition in gas-dominated systems with free water

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Fuel 2019年 236卷 870-879页

作者： Zhiyuan Wang Jing Yu Jianbo Zhang Shun Liu Yonghai Gao Hua Xiang Baojiang Sun Offshore Petroleum Engineering Research Center School of Petroleum Engineering China University of Petroleum (East China) Qingdao 266580 China Shaanxi Key Laboratory of Advanced Stimulation Technology for Oil&Gas Reservoirs Engineering Research Center of Development and Management for Low to Ultra-low Permeability Oil&Gas Reservoirs in West China (Ministry of Education) Xi’an Shiyou University Xi’an 710065 China National Engineering Laboratory for Subsea Equipment Testing and Detection Technology School of Petroleum Engineering China University of Petroleum (East China) Qingdao 266580 China Department of Development of Offshore Oil and Gas Fields Gubkin Russian State University of Oil and Gas (National Research University) Moscow 119991 Russia

The accurate prediction of temperature is the basis for predicting hydrate formation. Presently available methods for the calculation of temperature distributions in pipelines are based solely on the traditional heat transfer mechanism between the high-temperature fluid in the pipeline and the low-temperature surroundings. The influences of hydrate formation and deposition behaviors on the temperature variation have not been considered in the heat transfer process. Hydrate formation is exothermic, and the hydrate layer formed by the deposition of hydrates has a resistance effect to the heat transfer process. In this study, an improved thermal model for predicting the temperature distribution in gas-dominated systems is proposed. In this model, the influences of hydrate formation and deposition behaviors on the variation in temperature are considered. The model is verified by comparing with experimental data from literature. The transient variation in temperature along the pipeline can be obtained using the model. The simulation results indicate that hydrate formation and deposition have a significant influence on the variation in temperature in the pipeline, and can cause an inhibiting effect on the later hydrate formation. This work adds further insight into the heat transfer process and can serve as useful reference for the accurate prediction of temperature distributions in pipelines.

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grxB gene as a potential target for the development of Cronobacter sakazakii detection method in infant formula milk using real-time PCR

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AIP Conference Proceedings 2024年第1期2982卷

作者： M. Nurjayadi D. A. Juliansyah J. L. Declan G. I. Putri I. Krisdawati M. Azzahra I. Maulana A. M. A. Putri I. R. Kartika F. Kurniadewi D. Sukmawati S. Rahayu V. Saamia D. A. O. Saputro I. M. Wiranatha H. E. Enshasy Department of Chemistry Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Gedung K.H. Hasjim Asj'ari 6th Floor Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Research Center for Detection of Pathogenic Bacteria Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Negeri Jakarta Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Department of Biology Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Building K.H. Hasjim Asj'ari 9th Floor Rawamangun Muka Street East Jakarta 13220 Indonesia. Center Forensic Laboratory of the Criminal Investigation Police of the Republic of Indonesia Ciapmbuan Babakan Madang Bogor 1681 Indonesia Institute of Bioproduct Development Universiti Teknologi Malaysia (UTM) Skudai Johor Barhru Malaysia. School of Chemical and Energy Engineering Faculty of Engineering Universiti Teknologi Malaysia (UTM) Skudai Johor Bharu Malaysia. City of Scientific Research and Technology Applications New Burg Al Arab Alexandria Egypt.

Cronobacter sakazakii is a pathogen that causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates, with reported case-fatality rates varying from 40 to 80 percent. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii. This study aims to get the primer pairs and optimal annealing temperature for the Polymerase Chain Reaction (PCR) method to detect C. sakazakii. The glutaredoxin B (grxB) gene was found suitable for detecting Cronobacter sakazakii, and a primer pair based on the grxB gene sequence was designed and synthesized. The genomic DNA of Cronobacter sakazakii strain ATCC 29544 was isolated and then used as a template for PCR assay. Optimization of running PCR conditions was carried out by varying the annealing temperature at 53-62°C, and the template DNA concentration of C. sakazakii is 53 ng/µL with a purity (A260/280) of 1,853. The result showed that the primer grxB gave optimal results at the annealing temperature range between 57-61°C and could amplify the grxB C. sakazakii fragment to produce an amplicon with a size of 151 base pairs (bp), and only a single band was formed. Furthermore, the primer design results and the optimum temperature obtained will be used to develop a sensitive and specific rapid detection method of C. sakazakii in food samples using real-time PCR.

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Optimal annealing temperature of Yersinia enterocolitica ymoA gene primers using the polymerase chain reaction method

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AIP Conference Proceedings 2024年第1期2982卷

作者： M. Nurjayadi G. I. Putri J. L. Declan I. Krisdawati D. A. Juliansyah M. Azzahra I. Maulana I. R. Kartika F. Kurniadewi N. K. Liman T. Fahriza D. Sukmawati S. Rahayu V. Saamia D. A. O. Saputro I. M. Wiranatha H. E. Enshasy Department of Chemistry Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Gedung KH. Hasjim Asj'ari 6th Floor Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Research Center for Detection of Pathogenic Bacteria Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Negeri Jakarta Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Department of Biology Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Building KH. Hasjim Asj'ari 9th Floor Rawamangun Muka Street East Jakarta 13220 Indonesia. Center Forensic Laboratory of the Criminal Investigation Police of the Republic of Indonesia Ciapmbuan Babakan Madang Bogor 1681 Indonesia Institute of Bioproduct Development Universiti Teknologi Malaysia (UTM) Skudai Johor Barhru Malaysia. School of Chemical and Energy Engineering Faculty of Engineering Universiti Teknologi Malaysia (UTM) Skudai Johor Bharu Malaysia. City of Scientific Research and Technology Applications New Burg Al Arab Alexandria Egypt.

The case of foodborne diseases has now become a serious global problem. Foodborne diseases occur due to the contamination of pathogenic bacteria. One of the pathogenic bacteria that cause foodborne diseases is Yersinia enterocolitica. This research aimed to determine the annealing temperature of Yersinia enterocolitica ymoA gene primers using the PCR method to ensure that the primers can amplify ymoA gene fragments in Yersinia enterocolitica. In this research, the concentration of DNA as a template used was 70.5 ng/µL with a purity of A260/280 1.88. Optimization of the annealing temperature of Yersinia enterocolitica ymoA gene primers using the Gradient PCR in a range of temperatures between 53°C - 62°C based on the melting temperature value of the ymoA primers. The results of the primers design of the ymoA gene were confirmed to amplify the DNA fragment of Yersinia enterocolitica with an amplicon length of 185 bp. Based on the results, the temperature of 60°C was chosen as the optimum primer annealing temperature for the ymoA gene in Yersinia enterocolitica because only a brightness band was produced from the electrophoresis. This result can be continued with primers testing the ymoA gene on meat samples using the Real-Time PCR method.

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The potential of the ToxR gene as a detection tool for vibrio parahaemolyticus in seafood

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AIP Conference Proceedings 2024年第1期2982卷

作者： M. Nurjayadi I. Krisdawati J. L. Declan G. I. Putri D. A. Juliansyah M. Azzahra I. Maulana A. M. A. Putri I. R. Kartika F. Kurniadewi D. Sukmawati S. Rahayu V. Saamia D. A. O. Saputro I. M. Wiranatha H. E. Enshay Department of Chemistry Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Gedung KH. Hasjim Asj'ari 6th Floor Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Research Center for Detection of Pathogenic Bacteria Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Negeri Jakarta Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Department of Biology Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Building KH. Hasjim Asj'ari 9th Floor Rawamangun Muka Street East Jakarta 13220 Indonesia. Center Forensic Laboratory of the Criminal Investigation Police of the Republic of Indonesia Ciapmbuan Babakan Madang Bogor 1681 Indonesia Institute of Bioproduct Development Universiti Teknologi Malaysia (UTM) Skudai Johor Barhru Malaysia. School of Chemical and Energy Engineering Faculty of Engineering Universiti Teknologi Malaysia (UTM) Skudai Johor Bharu Malaysia. City of Scientific Research and Technology Applications New Burg Al Arab Alexandria Egypt.

Vibrio parahaemolyticus must be detected quickly and precisely. This study uses the Gradient Polymerase Chain Reaction (PCR) method to evaluate the primer that can produce an amplicon at the ideal temperature for the Vibrio parahaemolyticus ToxR gene. The ToxR primer pair could amplify Vibrio parahaemolyticus ToxR fragments to form an amplicon with size 171 base pairs, according to the in-silico analysis conducted for the primer design. Gradient PCR was used to assess the primer's suitability for the design outcome and determine the ideal temperature to generate amplicons of the appropriate size with temperatures ranging from 53 to 62°C. The purity (A260/280) of the Vibrio parahaemolyticus isolate used as a template is 1.92, and its concentration is 51 ng/µl. The study's findings kindly reveal that annealing temperatures of 58°C produce the best outcomes for the ToxR primer, which results in the formation of a clear DNA band with a thickness band intensity and a size of 171 base pairs. Additionally, the primers and ideal temperatures discovered were utilized for the Real-Time PCR stage in creating sensitive, targeted, and quicker tools for detecting Vibrio parahaemolyticus in Seafood.

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Horizontal position reconstruction in PandaX-II

arXiv

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arXiv 2021年

作者： Zhang, Dan Tan, Andi Abdukerim, Abdusalam Chen, Wei Chen, Xun Chen, Yunhua Cheng, Chen Cui, Xiangyi Fan, Yingjie Fang, Deqing Fu, Changbo Fu, Mengting Geng, Lisheng Giboni, Karl Gu, Linhui Guo, Xuyuan Han, Ke He, Changda He, Shengming Huang, Di Huang, Yan Huang, Yanlin Huang, Zhou Ji, Xiangdong Ju, Yonglin Li, Shuaijie Lin, Qing Liu, Huaxuan Liu, Jianglai Liu, Liqiang Lu, Xiaoying Ma, Wenbo Ma, Yugang Mao, Yajun Meng, Yue Namwongsa, Parinya Ni, Kaixiang Ning, Jinhua Ning, Xuyang Ren, Xiangxiang Shaheed, Nasir Shang, Changsong Shen, Guofang Si, Lin Wang, Anqing Wang, Hongwei Wang, Meng Wang, Qiuhong Wang, Siguang Wang, Wei Wang, Xiuli Wang, Zhou Wu, Mengmeng Wu, Shiyong Wu, Weihao Xia, Jingkai Xiao, Mengjiao Xiao, Xiang Xie, Pengwei Yan, Binbin Yang, Jijun Yang, Yong Yu, Chunxu Yuan, Jumin Yuan, Ying Zeng, Xinning Zhang, Tao Zhao, Li Zheng, Qibin Zhou, Jifang Zhou, Ning Zhou, Xiaopeng School of Physics and Astronomy Shanghai Jiao Tong University MOE Key Laboratory for Particle Astrophysics and Cosmology Shanghai Key Laboratory for Particle Physics and Cosmology Shanghai200240 China Department of Physics University of Maryland College ParkMD20742 United States Shanghai Jiao Tong University Sichuan Research Institute Chengdu610213 China Yalong River Hydropower Development Company Ltd. 288 Shuanglin Road Chengdu610051 China School of Physics Sun Yat-Sen University Guangzhou510275 China Tsung-Dao Lee Institute Shanghai200240 China School of Physics Nankai University Tianjin300071 China Institute of Modern Physics Fudan University Shanghai200433 China School of Physics Peking University Beijing100871 China School of Physics Beihang University Beijing100191 China International Research Center for Nuclei and Particles in the Cosmos Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang University Beijing100191 China School of Medical Instrument and Food Engineering University of Shanghai for Science and Technology Shanghai200093 China School of Mechanical Engineering Shanghai Jiao Tong University Shanghai200240 China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Hefei230026 China Department of Physics Princeton University PrincetonNJ United States Department of Modern Physics University of Science and Technology of China Hefei230026 China Shandong University Jinan250100 China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary Science Shandong University Shandong Qingdao266237 China Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai201210 China

Dual-phase noble-gas time projection chambers (TPCs) have improved the sensitivities for dark matter direct search in past decades. The capability of TPCs to reconstruct 3-D vertexes of keV scale recoilings is one of the most advantageous features. In this work, we develop two horizontal position reconstruction algorithms for the PandaX-II dark matter search experiment using the dual-phase liquid xenon TPC. Both algorithms are optimized by the 83mKr calibration events and use photon distribution of ionization signals among photomultiplier tubes to infer the positions. According to the events coming from the gate electrode, the uncertainties in the horizontal positions are 3.4 mm (3.9 mm) in the analytical (simulation-based) algorithm for an ionization signal with several thousand photon electrons in the center of the TPC. © 2021, CC BY-NC-SA.

关键词： Galaxies

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Dark Matter Search Results from the PandaX-4T Commissioning Run

arXiv

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arXiv 2021年

作者： Meng, Yue Wang, Zhou Tao, Yi Abdukerim, Abdusalam Bo, Zihao Chen, Wei Chen, Xun Chen, Yunhua Cheng, Chen Cheng, Yunshan Cui, Xiangyi Fan, Yingjie Fang, Deqing Fu, Changbo Fu, Mengting Geng, Lisheng Giboni, Karl Gu, Linhui Guo, Xuyuan Han, Ke He, Changda He, Jinrong Huang, Di Huang, Yanlin Huang, Zhou Hou, Ruquan Ji, Xiangdong Ju, Yonglin Li, Chenxiang Li, Mingchuan Li, Shu Li, Shuaijie Lin, Qing Liu, Jianglai Lu, Xiaoying Luo, Lingyin Ma, Wenbo Ma, Yugang Mao, Yajun Shaheed, Nasir Ning, Xuyang Qi, Ningchun Qian, Zhicheng Ren, Xiangxiang Shang, Changsong Shen, Guofang Si, Lin Sun, Wenliang Tan, Andi Wang, Anqing Wang, Meng Wang, Qiuhong Wang, Shaobo Wang, Siguang Wang, Wei Wang, Xiuli Wu, Mengmeng Wu, Weihao Xia, Jingkai Xiao, Mengjiao Xiao, Xiang Xie, Pengwei Yan, Binbin Yan, Xiyu Yang, Jijun Yang, Yong Yu, Chunxu Yuan, Jumin Yuan, Ying Zhang, Dan Zhang, Minzhen Zhang, Peng Zhang, Tao Zhao, Li Zheng, Qibin Zhou, Jifang Zhou, Ning Zhou, Xiaopeng Zhou, Yong School of Physics and Astronomy Shanghai Jiao Tong University MOE Key Laboratory for Particle Astrophysics and Cosmology Shanghai Key Laboratory for Particle Physics and Cosmology Shanghai 200240 China Shanghai Jiao Tong University Sichuan Research Institute Chengdu610213 China Tsung-Dao Lee Institute Shanghai Jiao Tong University Shanghai 200240 China Yalong River Hydropower Development Company Ltd. 288 Shuanglin Road Chengdu610051 China School of Physics Sun Yat-Sen University Guangzhou510275 China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary Science Shandong University Shandong Qingdao266237 China Key Laboratory of Particle Physics and Particle Irradiation of Ministry of Education Shandong University Shandong Qingdao266237 China School of Physics Nankai University Tianjin300071 China Institute of Modern Physics Fudan University Shanghai 200433 China School of Physics Peking University Beijing100871 China School of Physics Beihang University Beijing100191 China International Research Center for Nuclei and Particles in the Cosmos Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang University Beijing100191 China School of Medical Instrument and Food Engineering University of Shanghai for Science and Technology Shanghai 200093 China Department of Physics University of Maryland College ParkMD20742 United States School of Mechanical Engineering Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Hefei230026 China Department of Modern Physics University of Science and Technology of China Hefei230026 China SJTU Paris Elite Institute of Technology Shanghai Jiao Tong University Shanghai 200240 China

We report the first dark matter search results using the commissioning data from PandaX-4T. Using a time projection chamber with 3.7-tonne of liquid xenon target and an exposure of 0.63 tonne·year, 1058 candidate events are identified within an approximate nuclear recoil energy window between 5 and 100 keV. No significant excess over background is observed. Our data set a stringent limit to the dark matter-nucleon spin-independent interactions, with a lowest excluded cross section (90% C.L.) of 3.8 × 10−47cm2 at a dark matter mass of 40 GeV/c2 Copyright © 2021, The Authors. All rights reserved.

关键词： Germanium alloys

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Light yield and field dependence measurement in PandaX-II dual-phase xenon detector

arXiv

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arXiv 2021年

作者： Huang, Zhou Abdukerim, Abdusalam Bo, Zihao Chen, Wei Chen, Xun Chen, Yunhua Cheng, Chen Cheng, Yunshan Cui, Xiangyi Fan, Yingjie Fang, Deqing Fu, Changbo Fu, Mengting Geng, Lisheng Giboni, Karl Gu, Linhui Guo, Xuyuan Han, Ke He, Changda He, Jinrong Huang, Di Huang, Yanlin Hou, Ruquan Ji, Xiangdong Ju, Yonglin Li, Chenxiang Li, Mingchuan Li, Shu Li, Shuaijie Lin, Qing Liu, Jianglai Lu, Xiaoying Luo, Lingyin Ma, Wenbo Ma, Yugang Mao, Yajun Meng, Yue Shaheed, Nasir Ning, Xuyang Qi, Ningchun Qian, Zhicheng Ren, Xiangxiang Shang, Changsong Shen, Guofang Si, Lin Sun, Wenliang Tan, Andi Tao, Yi Wang, Anqing Wang, Meng Wang, Qiuhong Wang, Shaobo Wang, Siguang Wang, Wei Wang, Xiuli Wang, Zhou Wu, Mengmeng Wu, Weihao Xia, Jingkai Xiao, Mengjiao Xiao, Xiang Xie, Pengwei Yan, Binbin Yan, Xiyu Yang, Jijun Yang, Yong Yu, Chunxu Yuan, Jumin Yuan, Ying Zhang, Dan Zhang, Minzhen Zhang, Peng Zhang, Tao Zhao, Li Zheng, Qibin Zhou, Jifang Zhou, Ning Zhou, Xiaopeng Zhou, Yong School of Physics and Astronomy Shanghai Jiao Tong University MOE Key Laboratory for Particle Astrophysics and Cosmology Shanghai Key Laboratory for Particle Physics and Cosmology Shanghai 200240 China School of Physics Beihang University Beijing100191 China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Hefei230026 China Department of Modern Physics University of Science and Technology of China Hefei230026 China International Research Center for Nuclei and Particles in the Cosmos Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang University Beijing100191 China School of Physics Peking University Beijing100871 China Yalong River Hydropower Development Company Ltd. 288 Shuanglin Road Chengdu610051 China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary Science Shandong University Shandong Qingdao266237 China Key Laboratory of Particle Physics and Particle Irradiation of Ministry of Education Shandong University Shandong Qingdao266237 China Department of Physics University of Maryland College ParkMD20742 United States Tsung-Dao Lee Institute Shanghai Jiao Tong University Shanghai 200240 China School of Mechanical Engineering Shanghai Jiao Tong University Shanghai 200240 China School of Physics Sun Yat-Sen University Guangzhou510275 China School of Physics Nankai University Tianjin300071 China Institute of Modern Physics Fudan University Shanghai 200433 China School of Medical Instrument and Food Engineering University of Shanghai for Science and Technology Shanghai 200093 China Shanghai Jiao Tong University Sichuan Research Institute Chengdu610213 China SJTU Paris Elite Institute of Technology Shanghai Jiao Tong University Shanghai 200240 China

The dual-phase xenon time projection chamber (TPC) is one of the most sensitive detector technology for dark matter direct search, where the energy deposition of incoming particle can be converted into photons and electrons through xenon excitation and ionization. The detector response to signal energy deposition varies significantly with the electric field in liquid xenon. We study the detector’s light yield and its dependence on the electric field in the PandaX-II dual-phase detector containing 580 kg liquid xenon in the sensitive volume. From our measurements, the light yield at electric fields from 0 V/cm to 317 V/cm is obtained for energy depositions up to 236 keV. Copyright © 2021, The Authors. All rights reserved.

关键词： Deposition

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The potential of inlA gene as a target detection for Listeria monocytogenes in vegetables sample using polymerase chain reaction method

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AIP Conference Proceedings 2024年第1期2982卷

作者： M. Nurjayadi J. L. Declan G. I. Putri I. Krisdawati D. A. Juliansyah M. Azzahra I. Maulana R. G. Anggraeni I. R. Kartika F. Kurniadewi D. Sukmawati S. Rahayu V. Saamia D. A. O. Saputro I. M. Wiranatha B. Abomoelak H. E. Enshasy Department of Chemistry Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Building KH. Hasjim Asj'ari 6 Floor Rawamangun Muka Street East Jakarta 13220 Indonesia. Research Center for Detection of Pathogenic Bacteria Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Negeri Jakarta Jl. Rawamangun Muka Jakarta Timur 13220 Indonesia Department of Biology Faculty of Mathematics and Natural Science Universitas Negeri Jakarta Building KH. Hasjim Asj'ari 9 Floor Rawamangun Muka Street East Jakarta 13220 Indonesia. Center Forensic Laboratory of the Criminal Investigation Police of the Republic of Indonesia Cipambuan Babakan Madang Bogor West Java 1681 Indonesia Arnold Palmer Hospital Paediatric Speciality Diagnostic Laboratory Orlando FL 32806 USA Institute of Bioproduct Development Universiti Teknologi Malaysia (UTM) Skudai Johor Bahru Malaysia. School of Chemical and Energy Engineering Faculty of Engineering Universiti Teknologi Malaysia (UTM) Skudai Johor Bahru Malaysia. City of Scientific Research and Technology Applications New Burg Al Arab Alexandria Egypt.

Listeria monocytogenes is a bacteria that causes food poisoning and is found in various types of food. It can infect humans with a mortality rate of up to 30%. This study aims to determine the potential of the internalin A (inlA) gene with Gradient Polymerase Chain Reaction (PCR) as a basis for developing a detection method for Listeria monocytogenes in vegetables using Real-Time PCR. This study obtained a round white colony on the Brain Heart Infusion (BHI) agar. In liquid media, the OD600 value is 1.418, indicating the bacteria's growth. The DNA used as a template had a concentration of 72 ng/µL with A260/280 purity of around 1.802. Variation of annealing temperature in Gradient PCR between 53 – 62°C based on melting temperature of the primer. The primer successfully amplifies the inlA gene fragment with an amplicon size 161 base pair. In addition, the optimum annealing temperature for these primers was 55-60°C based on the bright band and a single band formed in electrophoresis. In the future, primers and optimum annealing temperature can be used to develop a detection kit for Listeria monocytogenes bacteria in vegetables using the Real-Time PCR method.

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