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Deep-learning-assisted medium optimization improves hyaluronic acid production by Streptococcus zooepidemicus

Journal of Bioscience and Bioengineering 2025年第6期139卷 429-435页

作者： Watanabe, Kazuki Kawai, Yoshizumi Kagenishi, Tomoko Chiou, Tai-Ying Konishi, Masaaki Department of Biotechnology and Environmental Chemistry of Engineering Kitami Institute of Technology 165 Koen-cho Hokkaido Kitami090-8507 Japan Biotechnology and Food Chemistry Course Program School of Regional Innovation and Social Design Engineering Kitami Institute of Technology 165 Koen-cho Hokkaido Kitami090-8507 Japan

To improve the efficiency of hyaluronic acid production by Streptococcus zooepidemicus, the growth medium was optimized with a pipeline involving a deep learning (DL) algorithm. To train the DL model, the initial training dataset (OA01–18) was designed with the L18 orthogonal array, and hyaluronic acid (HA) was produced in small-scale cultures in deepwell plates. The range of HA production was 0.09–1.39 g/L under these conditions. In searching for the optimal medium composition, 54 candidate optimized media (OM01–54) were proposed by the system. According to the confirming culture experiment, the best production of HA (1.66 g/L) was achieved with OM30. During confirmation in a stirred-tank reactor, the volumetric production of HA in OA30 was larger than that in the control medium. In fed batch culture, HA accumulated to 5.13 and 9.96 g/Linitial volume after 10 and 30 h in culture, respectively. To avoid the suppression of HA production by the high viscosity of the medium conferred by HA, repeated batch culture with OM30 was performed by replacing 90 % of the broth volume approximately every 6 h. As a result, 21.4 g of HA was produced in 46 h, and productivity reached 0.465 g/Linitial volume/h. © 2025 The Society for Biotechnology, Japan

关键词： Batch cell culture

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Leveraging ROTI map derived from Indonesian GNSS receiver network for advancing study of Equatorial Plasma Bubble in Southeast/East Asia

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Earth and Planetary Physics 2025年第1期9卷 101-116页

作者： Prayitno Abadi Ihsan N.Muafiry Teguh N.Pratama Angga Y.Putra Suraina Gatot H.Pramono Sidik T.Wibowo Febrylian F.Chabibi Umar A.Ahmad Wildan P.Tresna Asnawi Research Center for Climate and Atmosphere Indonesian National Research and Innovation Agency(BRIN)BandungIndonesia School of Electrical Engineering Telkom UniversityBandungIndonesia Directorate of Laboratory Management Facilities and Science and Technology Park Indonesian National Research and Innovation Agency(BRIN)PontianakIndonesia Geophysics Laboratory Master Program in PhysicsUniversitas Gadjah Mada(UGM)YogyakartaIndonesia Research Center for Space Indonesian National Research and Innovation Agency(BRIN)BandungIndonesia Indonesian Geospatial Information Agency(BIG) JakartaIndonesia Research Center for Photonics Indonesian National Research and Innovation Agency(BRIN)Tangerang SelatanIndonesia Department of Earth and Planetary Sciences Hokkaido UniversitySapporoJapan

This paper highlights the crucial role of Indonesia’s GNSS receiver network in advancing Equatorial Plasma Bubble(EPB)studies in Southeast and East Asia,as ionospheric irregularities within EPB can disrupt GNSS signals and degrade positioning *** by the Indonesian Geospatial Information Agency(BIG),the Indonesia Continuously Operating Reference Station(Ina-CORS)network comprises over 300 GNSS receivers spanning equatorial to southern low-latitude ***-CORS is uniquely situated to monitor EPB generation,zonal drift,and dissipation across Southeast *** provide a practical tool for EPB research,by sharing two-dimensional rate of Total Electron Content(TEC)change index(ROTI)derived from this *** generate ROTI maps with a 10-minute resolution,and samples from May 2024 are publicly available for further scientific *** preliminary findings from the ROTI maps of Ina-CORS are ***,the Ina-CORS ROTI maps reveal that the irregularities within a broader EPB structure persist longer,increasing the potential for these irregularities to migrate farther ***,we demonstrate that combined ROTI maps from Ina-CORS and GNSS receivers in East Asia and Australia can be used to monitor the development of ionospheric irregularities in Southeast and East *** have demonstrated the combined ROTI maps to capture the development of ionospheric irregularities in the Southeast/East Asian sector during the G5 Geomagnetic Storm on May 11,*** observed simultaneous ionospheric irregularities in Japan and Australia,respectively propagating northwestward and southwestward,before midnight,whereas Southeast Asia’s equatorial and low-latitude regions exhibited irregularities *** sharing ROTI maps from Indonesia and integrating them with regional GNSS networks,researchers can conduct comprehensive EPB studies,enhancing the understanding of EPB behavior across Southeast and East Asia and contributing significantly to ionosphe

关键词： Equatorial Plasma Bubble(EPB) GNSS receivers’network Indonesia Continuously Operating Reference Station(Ina-CORS) ionospheric map Rate of TEC change index(ROTI)map

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Structure-based design of new anticancer N3-Substituted quinazolin-4-ones as type I ATP-competitive inhibitors targeting the deep hydrophobic pocket of EGFR

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Computers in Biology and Medicine 2025年 186卷 109640-109640页

作者： El-Mahdy, Ragaa Y. Galal, Noha Lotfy, Rahma Arafa, Reem K. Drug Design and Discovery Lab Helmy Institute of Medical Sciences Zewail City of Science Technology and Innovation Giza12578 Egypt Biomedical Sciences Program University of Science and Technology Zewail City of Science Technology and Innovation Giza12578 Egypt

Epidermal growth factor receptor (EGFR) is amongst the earliest targeted kinases by small-molecule inhibitors for the management of EGFR-positive cancer types. While a few inhibitors are granted FDA approval for clinical use, discovery of new inhibitors is still of merit to enhance ligand-binding stability and subsequent enzyme inhibition. Thus, a structure-based design approach was adopted to devise a new series of twenty-nine N3-substituted quinazolin-4-ones as type I ATP-competitive inhibitors targeting the deep hydrophobic pocket of EGFR. The most active compounds demonstrated potent IC50s against MDA-MB-231 and HepG2 cancer cells being comparable to or better than the reference drugs erlotinib and lapatinib. IC50s of 5f and 15a against MDA-MB-231 were 3.34 and 4.99, whilst those against HepG2 were 6.37 and 2.18 μM, respectively. Also, members of this series demonstrated selective cytotoxicity against cancer cell lines showing low toxicity on human skin fibroblast normal cells hFB-4. Both 5f and 15a also effectively inhibited EGFR with sub-micromolar respective IC50s of 0.07 and 0.12 μM. The two derivatives halted the cell cycle progression of treated cancer cells and induced apoptosis as affirmed by flow cytometry along with RT-PCR-determined overexpression of the pro-apoptotic genes p53, Caspase 3, and Bax. Notably, docking and molecular dynamics simulations of members of this series of quinazolin-4-one derivatives showed that analogs with a short linker at the N3 position of the quinazoline ring exemplified by 5f bind to the active form of EGFR with their terminal aryl ring dwelling in the BPI pocket similar to erlotinib, while those with a longer linker represented by 15a bind to the inactive form in a comparable manner to lapatinib lodging the terminal phenyl in the BPII pocket. © 2024 Elsevier Ltd

关键词： Enzyme inhibition

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Path Planning Method for Mobile Robots in Pedestrian-Friendly Environments Using Drone Bird's-Eye View

Path Planning Method for Mobile Robots in Pedestrian-Friendl...

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2025 IEEE/SICE International Symposium on System Integration, SII 2025

作者： Ye, Linghao Chugo, Daisuke Muramatsu, Satoshi Yokota, Sho Hashimoto, Hiroshi Kwansei Gakuin University Graduate School of Science and Technology Hyogo Sanda6691330 Japan Tokai University School of Information Science and Technology Kanagawa Hiratsuka2591292 Japan Toyo University Faculty of Science and Engineering Saitama Kawagoe3508585 Japan Advanced Institute of Industrial Technology Master Program of Innovation for Design and Engineering Shinagawa Tokyo1400011 Japan

ISBN: (纸本)9798331531614

The objective of this paper is to develop a robot system that can transmit the video taken by the drone to the computer in real time, use YOLO to recognize the image of pedestrians, and enable the ground robot to plan the path to avoid pedestrians. External sensors, such as laser range finders (LRF) and CCD cameras, are often used on mobile robots. As we all know, these sensors are susceptible to occlusion. Therefore, even if the mobile robot avoids the pedestrian in front of it, it is likely to collide with other pedestrians that cannot be detected due to occlusion. In this study, we used a drone to shoot videos at high altitudes, transferred the videos to a computer, used YOLO on the computer to perform image recognition of pedestrians around the robot, and passed the recognition results to ROS, which enabled it to plan a path to avoid pedestrians around it. As a result, showed that the videos shot at high altitudes could effectively identify pedestrians around the robot after being recognized by YOLO. © 2025 IEEE.

关键词： Drones

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Physicochemical and sensory properties of novel cookies enriched with soy and anchovy protein isolate (Stolephorus insularis)

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Applied Food Research 2025年第1期5卷

作者： Canti, Meda Putra, Maximilliam Febriand Hutagalung, Rory Anthony Hartanti, Anastasia Tatik Della, Florence Food Technology Study Program School of Bioscience Technology and Innovation Atma Jaya Catholic University of Indonesia Tangerang 15345 Indonesia Master of Biotechnology Study Program School of Bioscience Technology and Innovation Atma Jaya Catholic University of Indonesia Jakarta 12930 Indonesia

Novel formula cookies are needed to meet nutritional requirements in the community and prevent diseases related to diet. This study aimed to evaluate the physicochemical and sensory properties of cookies enriched with a combination of proteins from soy protein isolate (SPI) and anchovy protein isolate (API). This study included the preparation of API, cookie production, analysis of the nutritional composition, amino acids, physical properties (colour, spread ratio, baking loss, density, texture profile), and sensory evaluation of cookies. Cookies were made with a ratio of wheat flour and isolate protein (SPI and API) of 80:20. The addition of SPI and API with a ratio of 0 % SPI and 0 % API (F0), 100 % SPI and 0 % API (F1), 75 % SPI and 25 % API (F2), 50 % SPI and 50 % API (F3);25 % SPI and 75 % API (F4), 0 % SPI and 100 % API (F5). The ash and protein content of cookies were found to increase significantly along with the addition of API concentration (p ≤ 0.05). The amino acid profile of cookies met the recommended standards of the Agriculture Organization of the United Nations/World Health Organization/United Nations University (FAO/WHO/UNU) for adults and children, namely F1 of 62.5 %;F2 and F3 of 75 %, F4 and F5 of 87.5 %. The most abundant amino acids in cookies were glutamic acid, aspartic acid, and serine. The redness (a*), yellowness (b*), colour difference (Δ_E), spread ratio, and texture profiles of cookies increased, while the lightness (L*), baking loss and density values decreased with increasing API addition. SPI-API cookies were acceptable in all sensory attributes. The results showed that the developed cookies can improve nutritional status in adults or children. © 2025 The Authors

关键词： Anchovy protein isolate Cookies Physicochemical Sensory characteristics Soy protein isolate

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Exploring Generative AI for Dynamic Carrying Capacity Assessment in Mountainous and Cave Areas: A Framework Development Approach 10

Exploring Generative AI for Dynamic Carrying Capacity Assess...

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10th International Conference on Digital Arts, Media and technology and 8th ECTI Northern Section Conference on Electrical, Electronics, Computer and Telecommunications Engineering, ECTI DAMT and NCON 2025

作者： Putjorn, Pruet Putjorn, Jittasak Sutumma, Janyawath Putjorn, Tipsuda Sangchoey, Thanakrit Wachirasirodom, Rachakorn Sangchatkaew, Supparat Kaewuthai, Kanokwan Sawasdee, Hathairat Jitpakdee, Pit Suksamran, Apalai Dokchan, Thanawat Mae Fah Luang University Center of Excellence in AI and Emerging Technologies School of Applied Digital Technology Chiang Rai Thailand Silpakorn University Tourism Management Program Faculty of Management Science Petchaburi Thailand Silpakorn University Community Management Program Faculty of Management Science Petchaburi Thailand Rajabhat University Sustainable Agriculture Innovation Program Faculty of Agricultural Technology Phuket Thailand Rajamangala University of Technology Tawan-ok Tourism Management Program Faculty of Liberal Arts Bangkok Thailand Maejo University Program in Public Administration School of Administrative Studies Chiang Mai Thailand Faculty of Liberal Arts Maejo University Thai and Eastern Languages Section Chiang Mai Thailand

ISBN: (纸本)9798331543273

This study explores the potential of Generative Artificial Intelligence (GenAI) in developing a dynamic framework for assessing tourism carrying capacities in natural destinations, with a specific focus on mountainous and cave areas in Thailand. The proposed framework, termed SAFER (Sustainable Economic Development, Allocating Resources Optimally, Fostering Community Engagement, Enhancing Visitor Satisfaction, and Resource Conservation), integrates GenAI's advanced capabilities in creating data-driven and adaptive solutions to address the complexities of sustainable tourism management. Unlike traditional AI applications, which emphasize pattern recognition and decision-making, GenAI uniquely enables the creation of predictive and prescriptive tools tailored to specific ecological and cultural contexts. The study employs a mixed-methods approach, involving data collection from 14 pilot sites, stakeholder feedback sessions, and collaborative workshops. These methodologies were critical in refining the SAFER framework and identifying its usability and scalability for diverse tourism settings. By leveraging GenAI's ability to analyze and generate content dynamically, the framework offers innovative strategies to balance tourism growth with environmental conservation and community welfare. Results highlight the SAFER framework's applicability in managing tourism's carrying capacity in sensitive ecosystems, demonstrating how GenAI enhances decision-making processes and visitor management strategies. While the focus of this research is on mountainous and cave regions due to their ecological significance and pressing conservation challenges, the SAFER framework can be adapted to other natural and cultural *** study contributes to sustainable tourism research by introducing an adaptable framework that aligns technological innovation with conservation principles, promoting long-term ecological and social well-being. Policymakers, tourism managers, and conservationis

关键词： Risk perception

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Systematic Review on Work-Related Musculoskeletal Health Risks in Haemodialysis Nurses 26th

Systematic Review on Work-Related Musculoskeletal Health Ris...

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26th International Conference on Human-Computer Interaction, HCII 2024

作者： Tsao, Yu-Chung Pei, Yu-Cheng Liu, Kai-Fen Tseng, Kevin C. Department of Occupational Medicine Chang Gung Memorial Hospital Taoyuan Taiwan Department of Physical Medicine and Rehabilitation Chang Gung Memorial Hospital Taoyuan Taiwan Department of Industrial Design and Master Program of Innovation and Design National Taipei University of Technology Taipei Taiwan Product Design and Development Laboratory Taoyuan Taiwan

ISBN: (纸本)9783031768088

In Taiwan, there are 364,000 patients suffering from chronic kidney disease, with over 90% of them requiring haemodialysis to sustain their lives due to kidney function failure. However, the substantial demand for haemodialysis care has often resulted in nurses’ overwork. The nature of their work, which requires high concentration and puts them under constant high pressure for prolonged periods, significantly impacts the physical and psychological well-being of haemodialysis nurses. Despite this, only a limited number of studies have addressed the health problems caused by their work. Therefore, this study attempts to conduct a systematic literature review to investigate and summarise health problems and risk factors related to musculoskeletal disorders caused by the work of haemodialysis nurses. The study conducted a systematic literature search in PubMed and Web of Science databases, eventually including ten articles for discussion. The systematic review covers topics that may contribute to haemodialysis nurses’ health problems and musculoskeletal disorders. Furthermore, the study aims to analyse whether musculoskeletal symptoms among haemodialysis nurses are due to the specific nature of haemodialysis tasks, work intensity, stress, fatigue, and other factors. The research findings indicate that haemodialysis nurses experience significant work-related stress due to their unique responsibilities, labour-intensive and skill-intensive tasks, repetitive nature of their tasks, and improper posture. These factors may lead to musculoskeletal problems, with hand-related musculoskeletal discomfort twice as prevalent in haemodialysis nurses as in other specialities. Musculoskeletal disorders are one of the leading causes of absenteeism among haemodialysis nurses. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.

关键词： Ergonomics

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Structural analysis of a neutral polysaccharide ATP-1 from the red seaweed Asparagopsis taxiformis and its immunomodulatory effects on RAW264.7 macrophages

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Food Science and Human Wellness 2025年

作者： Kun Yang Baojun Xu Wei Hu Yifan Tang Amy Hui-Mei Lin Saiyi Zhong College of Food Science and Technology， Guangdong Ocean University， Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety，Guangdong Province Engineering Laboratory for Marine Biological Products， Guangdong Provincial Engineering Technology Research Center of Seafood Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control Food Science and Technology Program， Department of Life Sciences， BNU-HKBU United International College Singapore Institute of Food and Biotechnology Innovation (SIFBI) Shenzhen Research Institute， Guangdong Ocean University

As a seaweed supplement， the powdered， entire herb of Asparagopsis taxiformis can effectively promote immune response in animals. However， the active factors responsible for this effect are currently unknown. A novel polysaccharide， ATP-1， was isolated and purified from the red alga A. taxiformis， and its structural and immunomodulatory properties were determined. ATP-1 is a neutral polysaccharide with a molecular weight of 307.978 kDa and is primarily composed of galactose (94.07%)， glucose (1.27%)， and xylose (4.66%). Scanning electron microscopy and atomic force microscopy revealed that ATP-1 had an amorphous structure and was composed of molecular chain entanglement and intramolecular hydrogen bonding interactions. The microstructure of ATP-1 exhibited asymmetric sheet–like cracks and pores. Results from immunomodulatory activity showed that ATP-1 promoted inducible nitric oxide (NO) synthase and cyclooxygenase (COX)-2 protein expression in RAW264.7 macrophages， improved macrophage phagocytic ability， and stimulated cytokine production (NO， tumor necrosis factor [TNF]-α， and interleukin [IL]-6). Quantitative proteome analysis identified 178 differentially expressed proteins (70 upregulated and 108 downregulated) that were involved in various molecular pathways， among which， TNF was one of the 20 most common and significant signaling pathways in inflammation and immunoregulation. Western blotting indicated that ATP-1 significantly upregulated the expression of IKKα， IKKβ， COX-2， and TNF-α proteins in the TNF pathway. In contrast， ATP-1 downregulated the expression of IκBα， Fas， and AIPI. Results from molecular docking suggested that hydrogen bonding predominantly contributed to the affinity between TNF-α and galactose， glucose， and xylose. These findings indicated that ATP-1 may play an immunomodulatory role by activating the TNF signaling pathway. ATP-1 enhanced macrophage phagocytic capacity and stimulated the production of cytokines NO， TNF-α， and IL-6， thereb

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Analysis of the Musculoskeletal Load Generated when a Small Dog Walks on a Slippery Floor

Analysis of the Musculoskeletal Load Generated when a Small ...

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2025 IEEE/SICE International Symposium on System Integration, SII 2025

作者： Li, Shijian Chugo, Daisuke Muramatsu, Satoshi Yokota, Sho She, Jin-Hua Hashimoto, Hiroshi Takashi, Uemura Kamishina, Hiroaki Hata, Yoshiharu Yamada, Takayuki Uchida, Takahiro Kwansei Gakuin University Graduate School of Science and Technology Hyogo Sanda6691330 Japan Tokai University School of Information Science and Technology Kanagawa Hiratsuka2591292 Japan Toyo University Faculty of Science and Engineering Saitama Kawagoe3508585 Japan Tokyo University of Technology School of Engineering Hachioji Tokyo1920982 Japan Advanced Institute of Industrial Technology Master Program of Innovation for Design and Engineering Shinagawa Tokyo1400011 Japan KyotoAR Animal Advanced Medical Center Kuse Kyoto6130034 Japan Rinrei Co. Ltd. Chuo Tokyo1040061 Japan

ISBN: (纸本)9798331531614

The objective of this paper is to identify the stresses placed on the musculoskeletal system when small dogs walk on floors with low friction coefficients. In many households, and small dogs in particular are often kept indoors. According to veterinary experience, it is known that small dogs kept indoors that walk with their lower limbs slipping cause a great deal of stress on the musculoskeletal system, and that the incidence of joint disease in the lower limbs of such small dogs is high. In this study, we measured the body movements of small dogs walking on slippery floors using a three-dimensional motion capture system and force plate system. Furthermore, by inputting these measurements into the musculoskeletal simulation model we have developed to simulate the musculoskeletal system of dogs, we derived the load on the musculoskeletal system of small dogs on slippery and non-slippery floors. As a result, it was identified that walking on a slippery floor causes a strong load on the lower limb muscles of small dogs. © 2025 IEEE.

关键词： Friction

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Multi-Partner Project: Enabling Digital Technologies for Holistic Health-Lifestyle Motivational and Assisted Supervision Supported by Artificial Intelligence Network (H2TRAIN)

Multi-Partner Project: Enabling Digital Technologies for Hol...

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Design, Automation and Test in Europe Conference and Exhibition

作者： Juan A. Montiel-Nelson Marco Ottella Paolo Azzoni Institute for Applied Microelectronics Univ. of Las Palmas de Gran Canaria Las Palmas de G.C. Spain Research and Innovation Xtremion Technology Velden Austria European Technology Program EUROTECH Group Amaro Italy

ISBN: (数字)9783982674100

ISBN: (纸本)9798331534646

H2TRAIN aligns with the ECS Strategic Research and innovation Agenda 2023 (ECS-SRIA), addressing key challenges in integrating digital technologies for health-focused lifestyles through AI-enhanced networks. This project pioneers the use of graphene to develop autonomous biosensors within CMOS technology, supporting advancements in AI-powered health services and IoT applications. Beyond digital integration, H2TRAIN innovates in energy detection, collection, and storage, essential for embedding health and sports functions in IoT wearables through smart textile and system integration. The solutions will be rigorously tested and validated with insights from medical, sports, social sciences, and end-user feedback. Focused on remote assisted living, amateur sports training, and post-operative monitoring, H2TRAIN aims to drive innovation in the smart healthcare sector, where investment in semiconductor nanofabrication is limited by the small scale of medical applications.

关键词： Technological innovation Image edge detection Real-time systems Assisted living Internet of Things Artificial intelligence Monitoring Sports System of systems Edge computing

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