检索结果-内蒙古大学图书馆

American Control Conference (ACC)

作者： Jonathan Vieth Marcel Reith-Braun Albert Bauer Florian Pfaff Georg Maier Robin Gruna Thomas Längle Harald Kruggel-Emden Uwe D. Hanebeck Intelligent Sensor-Actuator-Systems Laboratory Karlsruhe Institute of Technology Karlsruhe Germany Intelligent Sensor-Actuator-Systems Laboratory (ISAS) Karlsruhe Institute of Technology Karlsruhe Germany Chair of Mechanical Process Engineering and Solids Processing (MVTA) Technical University of Berlin Berlin Germany Fraunhofer Institute of Optronics System Technologies and Image Exploitation (IOSB) Karlsruhe

Optical sorting is a key technology for the circular economy and is widely applied in the food, mineral, and recycling industries. Despite its widespread use, one typically resorts to expensive means of adjusting the accuracy, e.g., by reducing the mass flow or changing mechanical or software parameters, which typically requires manual tuning in a lengthy, iterative process. To circumvent these drawbacks, we propose a new layout for optical sorters along with a controller that allows re-feeding of controlled fractions of the sorted mass flows. To this end, we build a dynamic model of the sorter, analyze its static behavior, and show how material recirculation affects the sorting accuracy. Furthermore, we build a model predictive controller (MPC) employing the model and evaluate the closed-loop sorting system using a coupled discrete element–computational fluid dynamics (DEM–CFD) simulation, demonstrating improved accuracy.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

引用

STAR Protocols 2024年第2期5卷 103106页

作者： Schmitz, Julian Yermakov, Boris Grünberger, Alexander Multiscale Bioengineering Faculty of Technology Bielefeld University Universitätsstraße 25 Bielefeld 33615 Germany Institute of Process Engineering in Life Sciences Microsystems in Bioprocess Engineering Karlsruhe Institute of Technology (KIT) Fritz-Haber-Weg 2 Karlsruhe 76131 Germany

microfluidic single-cell cultivation (MSCC) is a powerful tool for investigating the cellular behavior of various cell types at the single-cell level. Here, we present a protocol specifically developed for the reliable and reproducible MSCC of industrially relevant Chinese hamster ovary (CHO) suspension cell lines. We summarize critical experimental steps from the initial seed train up to the final MSCC experiment, with a special focus on pre-culture management and medium preparation, device inoculation, and the establishment of a constant medium perfusion. © 2024 The Author(s)

关键词： Biotechnology and bioengineering Cell culture Single Cell

来源：评论

学校读者我要写书评

暂无评论

Lipid, biomass productivity and growth rates of freshwater picoplankton Nannochloropsis limnetica SAG 18.99 cultivated in variant nitrate concentrations

引用

Journal of Cellular Biotechnology 2020年第1期6卷 1-11页

作者： Garus-Oas, Carol Hermionee Posten, Clemens Karlsruhe Institute of Technology Institute for Bio and Food Technology Area III: Bio Process Engineering Karlsruhe Germany

BACKGROUND: microalgae species for industrialization are largely selected because of their high lipid and biomass productivity. Both marine and freshwater species exhibit variations in biochemical compositions (i.e. lipid, carbohydrate and protein accumulation in biomass) when cultivated under varying environmental conditions. There is very little research available on the physiological responses of N. limnetica SAG 18.99 in terms of growth rates, biomass and lipid productivity when cultivated under variant nitrogen concentrations. OBJECTIVES: The objective of this research was to observe the physiological responses of Nannochloropsis limnetica (N. limnetica SAG 18.99) in terms of growth rates, biomass and neutral intracellular lipid when cultivated under variant nitrate concentrations. The null hypothesis was there is no significant difference in growth rate, biomass and neutral intracellular lipid productivity of N. limnetica SAG 18.99 cultivated under variant nitrate concentrations. METHODS: N. limnetica SAG 18.99 was cultivated under 'normal nitrate' (3.53M), 'nitrate replete' (7.06M), 'moderate nitrate deplete' (1.765M), and 'high nitrate deplete' (0.8825M) under the same conditions of light, pH, temperature and CO2 concentration over the duration of 14 days. The parameters measured during the cultivation were optical density to measure growth rates, flow-cytometry to measure cell concentrations/density, gravimetrical measurements for bio dry mass/biomass (BDM), ion chromatography measurements for ions/macronutrients, pH as well as sterility tests. All statistical analyses were performed using the SPSS software package (IBM statistics Version 23) and differences in data were considered significant at p<0.05. RESULTS: Growth rates were statistically significant (p=0.001). Therefore, the null hypothesis was rejected. Bio dry mass was not significant (p=0.939). Therefore, the null hypothesis was accepted. Relative fluorescence data recorded for all of the four fla

关键词： biomass growth rate microalgae Nannochloropsis limnetica (N. limnetica SAG 18.99) neutral intracellular lipid

来源：评论

学校读者我要写书评

暂无评论

Machine learning for rapid discovery of laminar flow channel wall modifications that enhance heat transfer

arXiv

引用

arXiv 2021年

作者： Koide, Yuri Kaithakkal, Arjun J. Schniewind, Matthias Ladewig, Bradley P. Stroh, Alexander Friederich, Pascal Institute of Theoretical Informatics Karlsruhe Institute of Technology Karlsruhe Germany Institute of Fluid Mechanics Karlsruhe Institute of Technology Karlsruhe Germany Institute for Micro Process Engineering Karlsruhe Institute of Technology Karlsruhe Germany Institute of Nanotechnology Karlsruhe Institute of Technology Karlsruhe Germany

Numerical simulation of fluids plays an essential role in modeling many physical phenomena, which enables technological advancements, contributes to sustainable practices, and expands our understanding of various natural and engineered systems. The calculation of heat transfer in fluid flow in simple flat channels is a relatively easy task for various simulation methods. However, once the channel geometry becomes more complex, numerical simulations become a bottleneck in optimizing wall geometries. We present a combination of accurate numerical simulations of arbitrary, flat, and non-flat channels and machine learning models predicting drag coefficient and Stanton number. We show that convolutional neural networks (CNN) can accurately predict the target properties at a fraction of the time of numerical simulations. We use the CNN models in a virtual high-throughput screening approach to explore a large number of possible, randomly generated wall architectures. Data Augmentation was applied to existing geometries data to add generated new training data which have the same number of parameters of heat transfer to improve the model's generalization. The general approach is not only applicable to simple flow setups as presented here but can be extended to more complex tasks, such as multiphase or even reactive unit operations in chemical engineering. © 2021, CC BY.

关键词： Geometry

来源：评论

学校读者我要写书评

暂无评论

Spin-correlation transport and multiple resistive states in multiferroic tunnel junctions

引用

Physical Review B 2024年第2期110卷 024428-024428页

作者： Xiaolin Zhang Lei Yin Sicong Zhu Ruiqing Cheng Yao Wen Jun He Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education and School of Physics and Technology Hubei Province Key Laboratory of Systems Science in Metallurgical Process The State Key Laboratory for Refractories and Metallurgy Collaborative Innovation Center for Advanced Steels International Research Institute for Steel Technology Department of Mechanical Engineering Wuhan Institute of Quantum Technology Wuhan 430206 China

Multiferroic tunneling junctions (MFTJs), which comprise magnetic electrodes and extremely thin ferroelectric tunneling barriers, are promising contenders for nonvolatile memory applications. Noncollinear antiferromagnetic Mn3Sn with time-reversal symmetry-breaking polarization properties and ferroelectric α−In2Se3 may open up the possibility of constructing room-temperature MFTJs. In this study, we investigate the spin-correlation transport in the MFTJs with Mn3Sn/BN/α−In2Se3/Mn3Sn structure using first-principles calculations. The resistance in this structure can be manipulated by tuning the directions of both the Néel vector of Mn3Sn and the electric polarization of the α−In2Se3 layer. Thus, multiple tunneling resistive states can be realized. We predict that huge tunneling magnetoresistance up to 6650% can be obtained by switching the magnetically oriented Néel vectors of Mn3Sn, and more than 8000% tunneling electrical resistance can be obtained by controlling the ferroelectric structure of α−In2Se3. Our work underscores the potential applications of Mn3Sn in multiferroic nonvolatile memories and lays the foundation for the development of ultrafast and efficient spintronic devices utilizing antiferromagnets.

关键词： Fermi surface Ferroelectricity Spintronics First-principles calculations

来源：评论

学校读者我要写书评

暂无评论

Plug & Produce – A 3D-printed sensor system concept combined with cloud-based process monitoring for data-driven decision support

引用

Procedia CIRP 2023年 118卷 348-353页

作者： Valeryia Sidarava Maximilian Backenstos Sebastian Rehfeldt Harald Klein Technical University of Munich TUM School of Engineering and Design Department of Energy and Process Engineering Institute of Plant and Process Technology Boltzmannstr. 15 85748 Garching Germany DatenBerg GmbH Haid-Und-Neu-Straße 7 76131 Karlsruhe Germany

Digitalization of industrial processes has become a more pressing issue in recent years. As complex industrial operations are improved by data-driven decision support, the availability of process data is a key requirement. This contribution describes a concept for flexible and cost-effective sensing systems and subsequent data processing. Application of additive manufacturing, IO-Link technology, and cloud-based analysis allows for high degrees of customizability and assembly simplification. An example is given for decision support facilitated by the aforementioned system in the area of process control. The proposed concept offers a simple plug-and-produce solution for cross-industry process monitoring and analytics.

关键词： Industry 4.0 process monitoring cloud-based data analysis sensing systems smart manufacturing

来源：评论

学校读者我要写书评

暂无评论

Hazard Analysis of Collaborative Automation Systems: A Two-layer Approach based on Supervisory Control and Simulation

Hazard Analysis of Collaborative Automation Systems: A Two-l...

引用

IEEE International Conference on Robotics and Automation (ICRA)

作者： Tom P. Huck Yuvaraj Selvaraj Constantin Cronrath Christoph Ledermann Martin Fabian Bengt Lennartson Torsten Kröger Intelligent Process Automation and Robotics Lab Institute of Anthropomatics and Robotics (IAR-IPR) Karlsruhe Institute of Technology India Department of Electrical Engineering Division of Systems and Control Chalmers University of Technology Gothenburg Sweden

Safety critical systems are typically subjected to hazard analysis before commissioning to identify and analyse potentially hazardous system states that may arise during operation. Currently, hazard analysis is mainly based on human reasoning, past experiences, and simple tools such as checklists and spreadsheets. Increasing system complexity makes such approaches decreasingly suitable. Furthermore, testing-based hazard analysis is often not suitable due to high costs or dangers of physical faults. A remedy for this are model-based hazard analysis methods, which either rely on formal models or on simulation models, each with their own benefits and drawbacks. This paper proposes a two-layer approach that combines the benefits of exhaustive analysis using formal methods with detailed analysis using simulation. Unsafe behaviours that lead to unsafe states are first synthesised from a formal model of the system using Supervisory Control Theory. The result is then input to the simulation where detailed analyses using domain-specific risk metrics are performed. Though the presented approach is generally applicable, this paper demonstrates the benefits of the approach on an industrial human-robot collaboration system.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Solute excretion improves trehalose uptake and desiccation tolerance of Metarhizium brunneum blastospores

引用

Biotechnology for the Environment 2025年第1期2卷 1-17页

作者： Dietsch, Robin Jakobs-Schönwandt, Desiree Blöbaum, Luisa Persicke, Marcus Grünberger, Alexander Patel, Anant WG Fermentation and Formulation of Biologicals and Chemicals Faculty of Engineering and Mathematics Bielefeld Germany Bioengineering and Sustainability Westfälische Hochschule Recklinghausen Germany Multiscale Bioengineering Technical Faculty Bielefeld University Bielefeld Germany OMICS Core Facility – Proteomics Metabolomics Unit (under construction) Bielefeld University Bielefeld Germany Microbial Genomics and Biotechnology Center for Biotechnology Bielefeld University Bielefeld Germany Institute of Process Engineering in Life Sciences: Microsystems in Bioprocess Engineering Karlsruhe Institute of Technology Karlsruhe Germany

In this study, a novel approach was developed to increase the intracellular trehalose concentration in M. brunneum blastospores by uptake from the medium, improving their desiccation tolerance. Briefly, placing the blastospores in a hypotonic environment induced solute excretion, allowing significantly increased trehalose uptake during subsequent trehalose treatment. Conductivity changes and GC–MS analysis revealed that glycerol and lactate excretion is triggered in a hypotonic NaCl solution. After hypotonic + trehalose treatment, intracellular glycerol, lactate, and glucose levels increased, while other desiccation-protective solutes remained unaffected. A fluorescence-based single-cell analysis of membrane stress and death during osmotic desiccation and rehydration showed that membrane stress and cell death were strongly amplified upon rehydration. Trehalose treatment reduced this amplification, while hypotonic + trehalose treatment minimised cell death and prevented a stress response during rehydration, though it increased membrane stress during desiccation. Newly formed germ tubes were identified as critical points of membrane failure during desiccation. Ultimately, the drying survival of blastospores was significantly improved by the hypotonic + trehalose treatment compared to untreated or trehalose-only-treated blastospores. In summary, this technique successfully enhanced blastospore desiccation tolerance and could be adapted to load cells with other solutes.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Customizable and Interactive Visualizations for Investigating Spatio-Temporal Single-Cell Information

Customizable and Interactive Visualizations for Investigatin...

引用

IEEE International Symposium on Biomedical Imaging

作者： Johannes Seiffarth Luisa Blöbaum Alexander Grünberger Katharina Nöh Institute of Bio- and Geosciences IBG-1: Biotechnology Forschungszentrum Jülich Jülich Germany Computational Systems Biotechnology (AVT.CSB) RWTH Aachen University Aachen Germany Multiscale Bioengineering Technical Faculty Bielefeld University Bielefeld Germany Institute of Process Engineering in Life Sciences Karlsruhe Institute of Technology Karlsruhe Germany

ISBN: (数字)9798350313338

ISBN: (纸本)9798350313345

Modern live-cell imaging and machine-learning based image processing methods shed light on the secrets of the behavior and characteristics of individual cells including their growth and development into colonies. However, the amount and complexity of spatio-temporal live-cell data makes gaining insights notoriously difficult. Therefore, we present novel visualizations that combine spatial and temporal information in interconnected and highly customizable view components. We demonstrate the flexibility of these components by designing experiment-specific visualizations at the example of three live-cell imaging data sets and gain insights into cell division, population formation and dynamic growth rates. The visualizations are implemented in Python notebooks and publicly available at https://***/JuBiotech/Supplement-to-Seiffarth-et-al.-ISBI-2024.

关键词： Image processing Sociology Data visualization Machine learning Complexity theory Statistics Biomedical imaging

来源：评论

学校读者我要写书评

暂无评论

Mechanistic Modeling of the Elution Behavior and Convective Entrapment of Vesicular Stomatitis Virus (Vsv) on an Ion Exchange (Iex) Chromatography Monolith

SSRN

引用

SSRN 2025年

作者： Schimek, Adrian Ng, Judy K.M. Rischawy, Federico Will Hubbuch, Jürgen ViraTherapeutics GmbH Bundesstraße 27 Rum6063 Austria Boehringer Ingelheim Pharma GmbH & Co.KG Birkendorfer Str. 65 Biberach88397 Germany Karlsruhe Institute of Technology Institute of Process Engineering in Life Sciences Section IV Biomolecular Separation Engineering Fritz-Haber-Weg 2 Karlsruhe76131 Germany

Developing a downstream purification process for replication-competent enveloped virus particles presents a significant challenge. This is largely due to the highly complex particle structures, as well as complexities of emerging purification modalities for such virus particles. In this study, an unexpected fluid-dynamic effect was observed during the elution of enveloped virus particles from an ion exchange chromatography monolith. This effect led to peak tailing and the separation of virus particle subpopulations. Upon considering possible causes, convective entrapment was identified as a plausible explanation. To investigate this effect, a mechanistic modeling approach representing the electrostatic resin interactions and the convective entrapment effect was implemented. The introduced Langmuir approximation of the convective entrapment showed good alignment with reference data. The model could not only reproduce the retention effect, but also suggested two virus particle populations due to the stronger retention effect on the tailing subpopulation caused by convective entrapment. © 2025, The Authors. All rights reserved.

关键词： Ion chromatography

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：