It is widely acknowledged that environmental literacy, in the early stages of education, can provide a strong foundation for future environmental behaviours, as well as help in the transition towards more sustainable ...
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Abstract During our screening for novel proteases, a Serratia sp. was isolated from alkaline soils the Himalayas, a biodiversity rich hotspot and identified according to polyphasic approach which includes morphologica...
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ISBN:
(纸本)9780816910731
Abstract During our screening for novel proteases, a Serratia sp. was isolated from alkaline soils the Himalayas, a biodiversity rich hotspot and identified according to polyphasic approach which includes morphological, biochemical and physiological characteristics and 16S rDNA sequence analysis. The protease was purified to homogeneity by acetone precipitation followed by gel filtration using Sephacryl S-100. The intact molecular mass of purified protease was determined by MALDI TOF and SDS-PAGE. The enzyme was highly active in the pH range of 6.0 to 11.0 and the optimum pH and temperature were found to be 7.5 and 50°C, respectively. The protease was characterized as serine protease based on the studies on class specific inhibitors and retained 92 % and 99 % of its activity in the presence of sodium perborate (0.5 %, w/v) and dodecyl benzene sulphonate (0.1 %, w/v), respectively. High stability in presence of detergents such as sodium dodecyl sulphate (0.1 %, w/v), Tween 80 (1.0 %, w/v), Triton X-100 (1.0 %, w/v) and organic solvents such as dimethyl sulfoxide, ethyl acetate and hexane (25 % and 50 % v/v) and its performance in stain removal makes this Serratia protease as an ideal choice for industrial applications such as in detergent, leather, food, pharmaceutical and chemical synthesis industries.
An integration of solar thermal energy can reduce the utility cost and the environmental impact. A proper integration of solar thermal energy is required in order to achieve it. The objective of this study is to maxim...
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A strain WHAK1, identified as Aspergillus niger, was isolated from Yichang phosphate mines in Hubei province of China. The fungus developed a phosphate solubilization zone on modified National Botanical research Insti...
A strain WHAK1, identified as Aspergillus niger, was isolated from Yichang phosphate mines in Hubei province of China. The fungus developed a phosphate solubilization zone on modified National Botanical researchinstitute's phosphate growth (NBRIP) agar medium, supplemented with tricalcium phosphate. The fungus was applied in a repeated-batch fermentation process in order to test its effect on solubilization of rock phosphate (RP). The results showed that A. niger WHAK1 could effectively solubilize RP in NBRIP liquid medium and released soluble phosphate in the broth, which can be illustrated by the observation of scanning electron microscope, energy-dispersive X-ray microanalysis, and Fourier transform infrared spectroscopy. Acidification of the broth seemed to be the major mechanism for RP solubilization by the fungus. Indeed, multiple organic acids (mainly gluconic acid) were detected in the broth by high-performance liquid chromatography analysis. These organic acids caused a significant drop of pH and an obvious rise of titratable acidity in the broth. The fungus also exhibited high levels of tolerance against temperature, pH, salinity, and desiccation stresses, although a significant decline in the fungal growth and release of soluble phosphate was marked under increasing intensity of stress parameters. Further, the fungus was introduced into the soil supplemented with RP to analyze its effect on plant growth and phosphate uptake of wheat plants. The result revealed that inoculation of A. niger WHAK1 significantly increased the growth and phosphate uptake of wheat plants in the RP-amended soil compared to the control soil.
The application of the tube hydroforming process for the manufacture of aerospace components is relatively new and challenging due to the limited formability of high strength alloys used for aerospace applications. A ...
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Thermomechanical Controlled processing (TMCP) including accelerated cooling after the final hot rolling pass is a well-established technology, widely applied in HSLA steel plate production. However, there are still ce...
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A significant level of cold cracking has been observed within direct chill (DC) cast, high-strength magnesium alloy Elektron WE43. These cracks have been attributed to the formation of significant residual stresses du...
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