检索结果-内蒙古大学图书馆

HSP90 and the cancer transcriptome: a comprehensive review of inhibitors and mechanistic insights

International Journal of Clinical Oncology 2025年 1-15页

作者： Shahana, M.V. Choudhary, Bibha Institute of Bioinformatics and Applied Biotechnology Electronic City Phase 1 Karnataka Bangalore 560100 India Research scholar Manipal Academy of Higher Education (MAHE) Manipal 576104 India

This review summarizes the structure, function, expression, and inhibitors of HSP90, the chaperone, in cancers. It systematically investigates the effects of HSP90 inhibitors, including AUY922, B11B021, CCT-018159, D7-gedunin, geldanamycin, and gedunin, across a range of cancer cell lines (HCC151, HT29, MCF7, PC3, VCAP, and A375) and a normal HA1E cell line, using data from the CLUE database. Our analysis reveals that treatment with these HSP90 inhibitors induces significant stress responses in tumor cells, initiating intrinsic and extrinsic apoptotic pathways. The HSP90AA1, HSP90AB1, HSP27, HSP70, VEGF, and NOTCH exhibited notable upregulation at 24 h post-treatment compared to 6 h, indicating a time-dependent increase in cellular stress (heat shock response) and activation of pro-survival signaling mechanisms. Additionally, the study highlights a significant upregulation of immune-related pathways, including those involving IL10, IL3, and IL7, following HSP90 inhibition, indicating that these inhibitors not only directly affect tumor cell viability but also modulate the tumor microenvironment by enhancing immune cell activation and cytokine release. The elevated levels of IL10 point to a dual role, where immune suppression mechanisms are also at play, potentially facilitating immune evasion by the tumor. The findings suggest that HSP90 inhibitors exhibit varying mechanisms of action across different cancer cell lines despite the presence of some common targets. These insights highlight the need for further investigation into the precise mechanisms of HSP90 inhibitors to optimize their therapeutic potential in different cancers. © The Author(s) under exclusive licence to Japan Society of Clinical Oncology 2025.

关键词： Combination therapy Drug resistance Heat shock protein Molecular chaperone Targeted therapy

来源：评论

学校读者我要写书评

暂无评论

Next-generation T cell-based in-silico multi-epitope vaccines: bridging cross-protection across diverse Shiga toxin variants

引用

Journal of Proteins and Proteomics 2025年第2期16卷 195-211页

作者： Dhanushkumar, T. Deepika, J. Aishwarya, C. S. Sunila, B. G. Doss, C. George Priya Vasudevan, Karthick Department of Biotechnology School of Applied Sciences REVA University Bengaluru India Laboratory of Integrative Genomics Department of Integrative Biology School of BioSciences and Technology Vellore Institute of Technology (VIT) Vellore India Manipal Academy of Higher Education (MAHE) Manipal India Institute of Bioinformatics International Technology Park Bangalore India

Shiga toxin-producing Escherichia coli (STEC) is a major public health concern due to its association with severe illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Vaccination offers a promising strategy to mitigate STEC carriage in cattle, thereby reducing human exposure. This study utilized a computational framework to design multi-epitope vaccine candidates targeting Shiga toxins. The key epitopes from Stx1A, Stx1cB, Stx2A, Stx2B, and Stx2f subunits were identified and prioritized based on immunogenicity and allergenicity profiles. Eight vaccine constructs were designed, incorporating diverse epitopes connected via linkers and adjuvants. Physicochemical analyses confirmed the constructs stability, solubility, and antigenicity. Secondary and tertiary structure predictions demonstrated accurate folding and structural reliability. Molecular docking revealed strong interactions with Toll-like receptor 4 (TLR4), with vaccine candidates VC-3 and VC-5 showing the highest binding affinities. Molecular dynamics simulations confirmed the stability and compactness of these complexes and revealed VC5 as the best stable complex. Codon optimization and in silico cloning ensured efficient expression in Escherichia coli. These findings highlight the potential of the proposed vaccine constructs to elicit robust immune responses and provide broad-spectrum protection against STEC infections. Experimental validation and preclinical studies are warranted to advance these candidates toward clinical applications.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Members of Enterobacterales Isolated from the Rice-Based Cropping System of the Sub-Himalayan Terai Region Promote Aggregation in Soil and Improve Seed Vigor

引用

Journal of Soil Science and Plant Nutrition 2025年 1-13页

作者： Pal, Srija Kolte, Vaishnavi Biswas, Nipa Pal, Poulomi Deb, Shovik Singh, Mohini Koul, Monika Sharma, Gaurav Karmakar, Kapudeep Department of Soil Science and Agricultural Chemistry Uttar Banga Krishi Viswavidyalaya Coochbehar 736165 India Institute of Bioinformatics and Applied Biotechnology Karnataka Bangalore 560100 India Department of Seed Science and Technology Uttar Banga Krishi Viswavidyalaya Coochbehar 736165 India School of Medical Science and Technology Indian Institute of Technology Kharagpur 721302 India Department of Life Science School of Basic Science and Research Sharda University Greater Noida 201310 India Department of Botany Hansraj College University of Delhi New Delhi 110007 India Department of Biotechnology Indian Institute of Technology Hyderabad Telangana 502284 India Regional Research Station Terai Zone Uttar Banga Krishi Viswavidyalaya Pundibari West Bengal Coochbehar 736165 India

The sub-Himalyan terai region of West Bengal, India, falls under the Cwa zone (according to Köppen climate classification). This region experiences high rainfall (> 3500 mm annually), which causes excessive soil erosion, loss of dissolved nutrients, and acidity in the soil. Many unexplored microbes native to this region have unknown ecological functions. This report discusses the order Enterobacterales and its members. Soil metagenome analysis and culture-dependent enumeration and isolation of Enterobacterales were used to determine their population yearly using a bile salt-containing medium. This work was carried out to find if these organisms have any alternate function in preserving the structure of soil and promoting seed vigor. The metagenome analysis from this region displayed the Enterobacterales as the 11th dominant order. Moreover, the dominance of these organisms, pinpoints that the native vegetation supports them in the soil. The results suggested that the presence of Serratia sp. and Enterobacter sp. in soil increased the soil macroaggregate formation due to their EPS-producing nature. Concomitantly, it was found that these bacteria can improve the seed vigor because of their Indole Acetic Acid-producing ability. Though the members of this order contain various bacteria whose presence in the soil indicates fecal contamination, the report lists some interesting functions like P-solubilization, aggregation, and phytohormone production. To the best of our knowledge, this is the first report showing the role of Serratia sp. in facilitating aggregate formation in soil. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2025.

关键词： Aggregation Enterobacterales High rainfall PGPR Soil acidity

来源：评论

学校读者我要写书评

暂无评论

Microbial disease resistance mechanisms with special reference to quorum sensing and quorum quenching: a review

引用

Discover biotechnology 2025年第1期2卷 1-29页

作者： Nachiyar, C. Valli Sunkar, Swetha Nellore, Jayshree Renugadevi, K. Namasivayam, S. Karthick Raja Bavanilatha, M. Prakash, P. Prabavathy, D. Sudha, S. Department of Research Meenakshi Academy of Higher Education and Research Chennai India Department of Bioinformatics Sathyabama Institute of Science and Technology Chennai India Department of Biotechnology Sathyabama Institute of Science and Technology Chennai India Department of Preventive Medicine UTHSC Memphis USA Centre for Applied Research Saveetha School of Engineering Saveetha Institute of Medical and Technical Sciences (SIMATS) Chennai India Faculty of Allied Health Sciences Shri Ramachandra Institute for Higher Education and Research Chennai India

Antimicrobial resistance (AMR) represents one of the most significant global health challenges of the twenty-first century. The emergence of drug-resistant pathogens, particularly multidrug-resistant (MDR) strains, threatens to undermine decades of progress in medicine, rendering infections once easily treatable with antibiotics increasingly difficult to manage. In addition, Climatic change and surrounding environmental conditions have led to a pragmatic shift to evolve through, adapt to new environments and surface new pathogenic emerging infectious diseases by existing pathogens. These pathogens acquire certain traits by various gene transfer mechanisms exhibiting wide host specificity and thereby influence surfacing epidemics or pandemics upon appropriate favourable conditions. Efforts to combat diseases are primarily focused on developing countries by medical sectors. This review summarizes various microbial disease resistance mechanisms, with emphasis on quorum sensing as one the important mechanism of resistance to drugs. In addition, advances in quorum sensing inhibitors/quenchers involving small molecules, bio-actives from microbial sources, natural compounds and quenching enzymes inhibiting microbial infections were discussed.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Strategies for enhancing interfacial built-in electric field strength in S-scheme heterojunction photocatalysts: A review

引用

Journal of Industrial and Engineering Chemistry 2025年 148卷 1-26页

作者： Ahmad, Irshad Lama Tamang, Tensangmu Ali, Ijaz Alanazi, Abdullah K. Farahim, Farha Ali, Talat Ahmad Khasawneh, Mohammad Rauf, Abdur Department of Physics University of Agriculture Faisalabad38040 Pakistan Department of Biotechnology Yeungnam University Gyeongbuk Gyeongsan38541 Korea Republic of Centre for Applied Mathematics and Bioinformatics Gulf University for Science and Technology Hawally32093 Kuwait Department of Chemistry College of Science Taif University Taif Saudi Arabia Department of Community Psychiatric and Mental Health Nursing King Khalid University Abha61413 Saudi Arabia Department of Basic Medical Sciences King Khalid University Abha61413 Saudi Arabia Department of Chemistry College of Science U.A.E. University Al-AinP.O. Box 15551 United Arab Emirates Institute of Physics Bahauddin Zakariya University Multan Pakistan

S-scheme heterojunctions, regarded as high-performance photocatalysts, have gained increasing attention in various applications due to their effective charge separation and migration, enhanced visible light absorption, high redox capacity, and remarkable stability. However, despite achieving effective charge separation, the built-in electric field (BIEF) driving charge separation process often lacks the desired strength. The BIEF strength is influenced by the Fermi level difference between the constituent semiconductors in an S-scheme heterojunction. Several emerging strategies have been explored to increase charge separation by enhancing the BIEF, particularly through modulation of band structure and Fermi levels. Despite significant progress in strengthening the BIEF, a review addressing these strategies is still lacking. This review consolidates recent advances in strategies to increase BIEF strength in S-scheme photocatalysts. Finally, the challenges and future perspectives for increasing BIEF strength through these strategies are discussed. This review provides valuable insights for researchers aiming to optimize charge separation and overall photocatalytic performance in S-scheme photocatalysts. © 2024 The Korean Society of Industrial and Engineering Chemistry

关键词： Fermi level

来源：评论

学校读者我要写书评

暂无评论

Multimodal evaluation of lipoxygenase-targeting NSAIDs using integrated in vitro, SAR, in silico, cytotoxicity towards MCF-7 cell line, DNA docking and MD simulation approaches

引用

International Journal of Biological Macromolecules 2025年 314卷 143665页

作者： Iqbal, Ambar Abbas, Wasim Ejaz, Samina Riaz, Naheed Ashok, Avinash Karkada Hayat, Muhammad Munawar Ashraf, Muhammad Institute of Chemistry Baghdad-ul-Jadeed Campus The Islamia University of Bahawalpur Bahawalpur 63100 Pakistan Department of Biochemistry Institute of Biochemistry Biotechnology & Bioinformatics (IBBB) B.J. Campus The Islamia University of Bahawalpur Bahawalpur 63100 Pakistan Health Biotechnology Division National Institute for Biotechnology and Genetic Engineering College Pakistan Institute of Engineering and Applied Sciences (NIBGE-C PIEAS) Punjab Faisalabad Pakistan Department of Biotechnology Siddaganga Institute of Technology Karnataka Tumakuru 572103 India Punjab Drug Testing Laboratory P & SH Department 1-Bird Wood Road Lahore Pakistan

Lipoxygenase (LOX) and cyclooxygenase (COX) pathways generate biologically active mediators implicated in inflammatory disorders and several classes of cancer. Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit the COX pathway by inhibiting the COX-1 and COX-2 enzymes. We reported earlier that several NSAIDs, including naproxen, aspirin and acetaminophen, inhibited lipoxygenase (LOX) enzyme at sub-micromolar concentrations. In continuation, the present work demonstrates the anti-LOX activity of nine more NSAIDs supported by in vitro, in silico, MD simulation and breast cancer cell line studies. All tested drugs displayed potent to excellent inhibitory profiles with IC₅₀ values <24.93 ± 0.64 μM. Aceclofenac (IC₅₀ 0.85 ± 0.06 μM) was the most active drug, followed by indomethacin (IC₅₀ 1.13 ± 0.07 μM), meloxicam (IC₅₀ 1.94 ± 0.07 μM) and ketorolac (IC₅₀ 9.26 ± 0.82 μM). Celecoxib (IC₅₀ 15.81 ± 0.71 μM), lornoxicam (IC₅₀ 16.54 ± 0.28 μM) and nimesulide (IC₅₀ 19.87 ± 0.85 μM) showed excellent inhibitory profiles. Flurbiprofen (IC₅₀ 21.73 ± 0.93 μM) and etoricoxib (IC₅₀ 24.93 ± 0.64 μM) moderately inhibited the target enzyme. SAR studies revealed that active molecules decorated with the carboxylate group afforded strong binding interactions as observed by in vitro assays and structural features. Other drugs, including enol derivatives and celecoxib, also showcased enhanced binding interactions. However, the cytotoxic effects of NSAIDs against the MCF-7 breast cancer cell line did not disclose significant anticancer activity. Molecular docking studies against human 5-LOX offered the best binding affinities for aceclofenac (−13.54 kcal/mol), accompanied by conventional hydrogen bonding and hydrophobic interactions as supported by the in vitro results. Docking studies with DNA dodecamer established minor groove binding with their possible role in DNA replication

关键词： 15-LOX inhibition DFT calculation DNA docking studies MCF-7 cell line MD simulations Molecular docking NSAIDs

来源：评论

学校读者我要写书评

暂无评论

Characterization and comparative profiling of piRNAs in serum biopsies of pediatric Wilms tumor patients

引用

Cancer Cell International 2025年第1期25卷 1-15页

作者： Mohamed, Fatma S. Jalal, Deena Fadel, Youssef M. El-Mashtoly, Samir F. Khaled, Wael Z. Sayed, Ahmed A. Ghazy, Mohamed A. Biotechnology Program Institute of Basic and Applied Science Egypt-Japan University of Science and Technology Alexandria New Borg El-Arab City Egypt Biochemistry ProgramFaculty of Science Minia University El-Minia Egypt Genomics and Metagenomics Program Department of Basic Research Children’s Cancer Hospital Egypt Cairo 57357 Egypt Bioinformatics Group Center for Informatics Science School of Information Technology and Computer Science Nile University Giza Egypt Leibniz Institute of Photonic Technology Albert Einstein-Straße 9 Jena 07745 Germany Department of Pediatric Oncology National Cancer Institute Cairo Egypt Department of Pediatric Oncology Children’s Cancer Hospital Egypt Cairo 57357 Egypt Consultant Pediatric Oncology Mouwasat Hospital Dammam Saudi Arabia Department of Biochemistry Faculty of Science Ain Shams University Cairo Egypt

Piwi-interacting RNAs (piRNAs) are small non-coding RNAs involved in transposon silencing and linked to cancer progression. However, their role in Wilms tumors (WT) remains unexplored. We conducted a thorough analysis and characterization of piRNAs in serum liquid biopsies of WT patients. Our study examined their expression patterns and functional annotations related to WT pathogenesis, as well as their clinical potential for diagnosis and monitoring. We identified 307 piRNAs expressed in WT serum samples, with 4% classified as repeat-related and 96% as non-repeat-related. The most abundant repeat-related piRNAs originated from LINEs retrotransposon, while tRNA-derived piRNAs were the most prevalent among non-repeat-related piRNAs. Furthermore, a distinct profile of 34 piRNAs showed significant differential expression in WT patients compared to healthy controls—22 downregulated and 12 upregulated. The target genes of differentially expressed piRNAs exhibited significant enrichment in biological pathways related to cytokine activity, inflammatory responses, TGF-beta signaling, p38 MAPK, and ErbB signaling. These genes are also involved in DNA damage response, DNA methylation, cell cycle regulation, as well as kidney development and function. Low expression levels of several piRNAs, especially piR-hsa-1,913,711, piR-hsa-28,190, piR-hsa-28,849, piR-hsa-28,848, and piR-hsa-28,318, showed significant diagnostic potential as non-invasive biomarkers for WT (AUC > 0.8, p < 0.05). Their expression levels also significantly correlated with adverse pathological features, including metastasis, anaplasia, and bilateral WT development. In conclusion, non-transposon-related piRNAs may serve as reliable biomarkers for WT and possess potential non-germline functions, particularly in regulating DNA methylation, cell growth, immune responses, and immune responses. Further studies are warranted to elucidate their functional significance. © The Author(s) 2025.

关键词： Blood-based biomarkers Diagnostic potential Non-germline functions Non-transposon-related PiRNAs PiRNAome profiling Wilms tumor

来源：评论

学校读者我要写书评

暂无评论

Correction to: Autophagy-related gene BECN1 single nucleotide polymorphisms in diseases

引用

Molecular and cellular biochemistry 2025年第6期480卷 2025 Jan 4页

作者： Sargeet Kaur Jitendraa Vashistt Ajay Kumar Jyoti Parkash Harish Changotra Department of Biotechnology and Bioinformatics Jaypee University of Information Technology Waknaghat Solan Himachal Pradesh 173 234 India. Translational Health Science and Technology Institute (THSTI) Faridabad Haryana 121001 India. Department of Zoology School of Basic and Applied Sciences Central University of Punjab Bathinda 151 001 India. Department of Molecular Biology and Biochemistry Guru Nanak Dev University Amritsar Punjab 143 005 India. hchangotra@***.

来源：评论

学校读者我要写书评

暂无评论

Bridging data gaps: African reference genomes advancing inclusive microbiome research

引用

Trends in microbiology 2025年第5期33卷 479-483页

作者： Soumaya Kouidhi Jo-Ann S Passmore Mathabatha Evodia Setati Lamech M Mwapagha Adijat Jimoh Ovokeraye H Oduaran Laboratory of Biotechnology and Valorisation of Bio-Geo Resources (LR11ES31) Higher Institute of Biotechnology of Sidi Thabet BiotechPole of Sidi Thabet University of Manouba Ariana Tunisia. Electronic address: soumaya.kouidhi@isbst.uma.tn. Institute of Infectious Disease and Molecular Medicine Department of Pathology University of Cape Town Cape Town South Africa National Health Laboratory Service Cape Town South Africa. South African Grape and Wine Research Institute Stellenbosch University Stellenbosch South Africa African Microbiome Institute Division of Molecular Biology and Human Genetics Department of Biomedical Sciences Stellenbosch University Stellenbosch South Africa. Department of Biology Chemistry and Physics Faculty of Health Natural Resources and Applied Sciences Namibia University of Science and Technology Private Bag 13388 13 Jackson Kaujeua Street Windhoek Namibia. Genetics Genomics and Bioinformatics Department National Biotechnology Research and Development Agency Abuja Nigeria. Sydney Brenner Institute for Molecular Bioscience University of the Witwatersrand Johannesburg South Africa.

The need for African-specific microbiome reference genomes is critical as their absence limits research in health, agriculture, and sustainability. Current global guidelines often do not take into account the region's unique needs. This commentary advocates for Africa-focused microbiome initiatives and community-driven genome projects to better reflect the continent's ecological and demographic diversity, leading to more applicable outcomes.

关键词： African-specific microbiome data sharing locally-led genome projects precision microbiome research reference genomes regional customization

来源：评论

学校读者我要写书评

暂无评论

From thermodynamics to protein design: Diffusion models for biomolecule generation towards autonomous protein engineering

arXiv

引用

arXiv 2025年

作者： Li, Wenran Cadet, Xavier F. Medina-Ortiz, David Davari, Mehdi D. Sowdhamini, Ramanathan Damour, Cedric Li, Yu Miranville, Alain Cadet, Frederic University of Paris City University of Reunion INSERM BIGR DSIMB ParisF-75015 France Department of Computing Imperial College London United Kingdom Department of Computer Engineering University of Magallanes Punta Arenas Chile Centre for Biotechnology and Bioengineering Universidad de Chile Santiago Chile Department of Bioorganic Chemistry Leibniz Institute of Plant Biochemistry Halle06120 Germany National Centre for Biological Science TIFR Bangalore India Molecular Biophysics Unit Indian Institute of Science Bangalore India Institute of Bioinformatics and Applied Biotechnology Bangalore India EnergyLab EA 4079 Faculty of Sciences and Technology University of Reunion France School of Information Science and Technology Beijing Institute of Artificial Intelligence China Laboratoire de Mathématiques Appliquées University Le Havre Normandie le Havre France PEACCEL Artificial Intelligence Department AI for Bi-ologics Paris75013 France

Protein design with desirable properties has been a significant challenge for many decades. Generative artificial intelligence is a promising approach and has achieved great success in various protein generation tasks. Notably, diffusion models stand out for their robust mathematical foundations and impressive generative capabilities, offering unique advantages in certain applications such as protein design. In this review, we first give the definition and characteristics of diffusion models and then focus on two strategies: Denoising Diffusion Probabilistic Models (DDPM) and Score-based Generative Models (SGM), where DDPM is the discrete form of SGM. Furthermore, we discuss their applications in protein design, peptide generation, drug discovery, and protein-ligand interaction. Finally, we outline the future perspectives of diffusion models to advance autonomous protein design and engineering. The E(3) group consists of all rotations, reflections, and translations in three-dimensions. The equivariance in the E(3) group can maintain the physical stability of the N − Cα − C frame of each amino acid as much as possible, and we reflect on how to keep the diffusion model E(3) equivariant for protein generation. © 2025, CC BY.

关键词： Biomolecules

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：