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real-time Cell Sorting with Scalable In Situ FPGA-Accelerated Deep learning

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arXiv 2025年

作者： Islam, Khayrul Forelli, Ryan F. Han, Jianzhong Bhadane, Deven Huang, Jian Agar, Joshua C. Tran, Nhan Ogrenci, Seda Liu, Yaling Department of Mechanical Engineering Lehigh University BethlehemPA18015 United States Department of Electrical and Computer Engineering Northwestern University EvanstonIL60208 United States Coriell Institute for Medical Research CamdenNJ United States Department of Computer Science Lehigh University BethlehemPA18015 United States Cooper Medical School of Rowan University CamdenNJ08103 United States Center for Metabolic Disease Research Temple University Lewis Katz School of Medicine PhiladelphiaPA19122 United States Department of Mechanical Engineering and Mechanics Drexel University PhiladelphiaPA19104 United States Real-time Processing Systems Division Fermi National Accelerator Laboratory BataviaIL60510 United States Department of Bioengineering Lehigh University BethlehemPA18015 United States

Precise cell classification is essential in biomedical diagnostics and therapeutic monitoring, particularly for identifying diverse cell types involved in various diseases. Traditional cell classification methods such as flow cytometry depend on molecular labeling which is often costly, time-intensive, and can alter cell integrity. To overcome these limitations, we present a label-free machine learning framework for cell classification, designed for real-time sorting applications using bright-field microscopy images. This approach leverages a teacher-student model architecture enhanced by knowledge distillation, achieving high efficiency and scalability across different cell types. Demonstrated through a use case of classifying lymphocyte subsets, our framework accurately classifies T4, T8, and B cell types with a dataset of 80,000 preprocessed images, accessible via an open-source Python package for easy adaptation. Our teacher model attained 98% accuracy in differentiating T4 cells from B cells and 93% accuracy in zero-shot classification between T8 and B cells. Remarkably, our student model operates with only 0.02% of the teacher model’s parameters, enabling field-programmable gate array (FPGA) deployment. Our FPGA-accelerated student model achieves an ultra-low inference latency of just 14.5 µs and a complete cell detection-to-sorting trigger time of 24.7 µs, delivering 12x and 40x improvements over the previous state-of-the-art real-time cell analysis algorithm in inference and total latency, respectively, while preserving accuracy comparable to the teacher model. This framework provides a scalable, cost-effective solution for lymphocyte classification, as well as a new SOTA real-time cell sorting implementation for rapid identification of subsets using in situ deep learning on off-the-shelf computing hardware. © 2025, CC BY.

关键词： Deep learning

Development of Efficient Wireless Sensor Network for IoT Applications

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Development of Efficient Wireless Sensor Network for IoT App...

Pervasive Computing and Social Networking (ICPCSN), International Conference on

作者： S. Shankar G. Deepika G. Devi S. Ramesh Suraj Srivastava S Sandeep Kumar Department of Electronics and Communication Systems Sri Krishna Arts and Science College Coimbatore Tamil Nadu India Department of Electronics and Communication Engineering St. Peter's Engineering College Hyderabad Telangana India Department of Electrical and Electronics Engineering PSNA College of Engineering and Technology Dindigul Tamil Nadu India Depattment of Computational Intelligence Institute of Artificial Intelligence and Machine Learning Saveetha University Chennai Tamil Nadu India Department of Computer science and Engineering I.K. Gujral Punjab Technical University SahibzadaAjit Singh Nagar Punjab India Department of Computer Science and Engineering Koneru Lakshmaiah Education Foundation Vaddeswaram Andhra Pradesh India

This research study provides an overview of the effort that went into developing reliable “Wireless sensor networks” for IoT applications, including both theoretical study and practical testing. The report also includes the findings of an analysis of variance and regression analysis, which pinpoint the significance of such aspects as network architecture, traffic load, and ambient conditions on the performance of Wireless Sensor Network (WSN). Finally, study provides a discussion on the future research directions, as well as the design and implementation of a Wireless Sensor Network (WSN) for Internet of Things (IoT) applications.

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Awareness in robotics: An early perspective from the viewpoint of the EIC Pathfinder Challenge "Awareness Inside"

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arXiv 2024年

作者： Santina, Cosimo Della Corbato, Carlos Hernandez Sisman, Burak Leiva, Luis A. Arapakis, Ioannis Vakalellis, Michalis Vanderdonckt, Jean D’Haro, LuisFernando Manzi, Guido Becchio, Cristina Elamrani, Aïda Alirezaei, Mohsen Castellano, Ginevra Dimarogonas, Dimos V. Ghosh, Arabinda Haesaert, Sofie Soudjani, Sadegh Stroeve, Sybert Verschure, Paul Bacciu, Davide Deroy, Ophelia Bahrami, Bahador Gallicchio, Claudio Hauert, Sabine Sanz, Ricardo Lanillos, Pablo Iacca, Giovanni Sigg, Stephan Gasulla, Manel Steels, Luc Sierra, Carles Department of Cognitive Robotics Delft University of Technology Delft Netherlands Oberpfaffenhofen Germany Department of Computer Science University of Luxembourg Luxembourg 4 Telefónica I+D Spain AEGIS IT Research GmbH Germany Université catholique de Louvain Belgium Speech Technology and Machine Learning Group ETSI de Telecomunicación Universidad Politécnica de Madrid Madrid Spain Alien Venture Studio Alien Technology Transfer Rome Italy Hamburg Germany Institut Jean Nicod École Normale Supérieure Paris France Research Technology and Development Department Digital Industry Software Helmond Netherlands Department of Information Technology Uppsala University Uppsala Sweden Division of Decision and Control Systems School of Electrical Engineering and Computer Science KTH Royal Institute of Technology Stockholm Sweden Max Planck Institute for Software Systems Kaiserslautern Germany Department of Electrical Engineering Control Systems Group Eindhoven University of Technology Eindhoven Netherlands Amsterdam Netherlands Donders Centre for Neuroscience Radboud University Nijmegen Netherlands Computer Science Department University of Pisa Pisa Italy Faculty of Philosophy Philosophy of Science The Study of Religion Ludwig Maximilian University of Munich Munich Germany School of Engineering Mathematics and Technology University of Bristol Bristol United Kingdom Autonomous Systems Laboratory Universidad Politécnica de Madrid Spain Madrid Spain Department of Information Engineering and Computer Science University of Trento Trento Italy Department of Information and Communications Engineering Aalto University Espoo Finland Department of Electronic Engineering Universitat Politècnica de Catalunya Barcelona Spain Studio Stelluti Brussels Belgium Barcelona Spain

Consciousness has been historically a heavily debated topic in engineering, science, and philosophy. On the contrary, awareness had less success in raising the interest of scholars in the past. However, things are changing as more and more researchers are getting interested in answering questions concerning what awareness is and how it can be artificially generated. The landscape is rapidly evolving, with multiple voices and interpretations of the concept being conceived and techniques being developed. The goal of this paper is to summarize and discuss the ones among these voices that are connected with projects funded by the EIC Pathfinder Challenge called "Awareness Inside", a nonrecurring call for proposals within Horizon Europe that was designed specifically for fostering research on natural and synthetic awareness. In this perspective, we dedicate special attention to challenges and promises of applying synthetic awareness in robotics, as the development of mature techniques in this new field is expected to have a special impact on generating more capable and trustworthy embodied systems. © 2024, CC BY.

关键词： Robotics

Enabling End-to-End Secure Federated learning in Biomedical Research on Heterogeneous Computing Environments with APPFLx

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arXiv 2023年

作者： Hoang, Trung-Hieu Fuhrman, Jordan Madduri, Ravi Li, Miao Chaturvedi, Pranshu Li, Zilinghan Kim, Kibaek Ryu, Minseok Chard, Ryan Huerta, E.A. Giger, Maryellen Department of Electrical and Computer Engineering Coordinated Science Laboratory University of Illinois at Urbana-Champaign Street UrbanaIL61801 United States University of Chicago ChicagoIL United States Data Science and Learning Division Argonne National Laboratory LemontIL United States Department of Computer Science University of Illinois at Urbana-Champaign Street UrbanaIL61801 United States School of Industrial and Systems Engineering Georgia Institute of Technology AtlantaGA United States School of Computing Augmented Intelligence Arizona State University TempeAZ United States

Facilitating large-scale, cross-institutional collaboration in biomedical machine learning projects requires a trustworthy and resilient federated learning (FL) environment to ensure that sensitive information such as protected health information is kept confidential. In this work, we introduce APPFLx, a low-code FL framework that enables the easy setup, configuration, and running of FL experiments across organizational and administrative boundaries while providing secure end-to-end communication, privacy-preserving functionality, and identity management. APPFLx is completely agnostic to the underlying computational infrastructure of participating clients. We demonstrate the capability of APPFLx as an easy-to-use framework for accelerating biomedical studies across institutions and healthcare systems while maintaining the protection of private medical data in two case studies: (1) predicting participant age from electrocardiogram (ECG) waveforms, and (2) detecting COVID-19 disease from chest radiographs. These experiments were performed securely across heterogeneous compute resources, including a mixture of on-premise high-performance computing and cloud computing, and highlight the role of federated learning in improving model generalizability and performance when aggregating data from multiple healthcare systems. Finally, we demonstrate that APPFLx serves as a convenient and easy-to-use framework for accelerating biomedical studies across institutions and healthcare system while maintaining the protection of private medical data. Copyright © 2023, The Authors. All rights reserved.

关键词： Privacy-preserving techniques

Spectroscopic detection of pigments in tissues: correlation with tissue aging and cancer development

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Spectroscopic detection of pigments in tissues: correlation ...

2022 International Conference Laser Optics, ICLO 2022

作者： Oliveira, L.M. Goncalves, T.M. Botelho, A.R. Martins, I.S. Silva, H.F. Carneiro, I. Carvalho, S. Henrique, R. Tuchin, V.V. Porto Portugal Center of Innovation in Engineering and Industrial Technology Isep Porto Portugal Department of Electrical and Computer Engineering Porto University-School of Engineering Porto Portugal Department of Pathology and Cancer Biology and Epigenetics Group Portuguese Oncology Institute of Porto Porto Portugal Porto Portugal Viana do Castelo Portugal Dept. of Pathol. and Molecular Immunology Porto University-Institute of Biomedical Sciences Abel Salazar Porto Portugal Science Medical Center Saratov State University Saratov Russia Laboratory of Laser Molecular Imaging and Machine Learning National Research Tomsk State University Tomsk Russia Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision Mechanics and Control Frc-Saratov Scientific Centre-of the Russian Academy of Sciences Saratov Russia

ISBN: (纸本)9781665466646

The direct calculation of the absorption coefficient spectra of various tissues from spectral measurements allowed to retrieve the contents of melanin and lipofuscin. In the rabbit brain cortex, 1.8 times higher melanin content is explained by the neuron degeneration process. Similar melanin and lipofuscin contents were found in the rabbit pancreas as a result of the tissue aging process. The conversion of 83 % of the melanin in the human normal kidney into lipofuscin in the cancer kidney indicates that lipofuscin can be considered a kidney cancer marker in humans. © 2022 IEEE.

关键词： Tissue

Vision-Language Models in Remote Sensing: Current Progress and Future Trends

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arXiv 2023年

作者： Li, Xiang Wen, Congcong Hu, Yuan Yuan, Zhenghang Zhu, Xiao Xiang The King Abdullah University of Science and Technology Thuwal23955 Saudi Arabia Department of Electrical and Computer Engineering New York University Abu Dhabi Abu Dhabi129188 United Arab Emirates The Institute of Remote Sensing and Geographic Information Systems Peking University Beijing100871 China Data Science in Earth Observation Technical University of Munich Munich80333 Germany The Munich Center for Machine Learning Munich80333 Germany

The remarkable achievements of ChatGPT and GPT-4 have sparked a wave of interest and research in the field of large language models for Artificial General Intelligence (AGI). These models provide intelligent solutions close to human thinking, enabling us to use general artificial intelligence to solve problems in various applications. However, in remote sensing (RS), the scientific literature on the implementation of AGI remains relatively scant. Existing AI-related research in remote sensing primarily focuses on visual understanding tasks while neglecting the semantic understanding of the objects and their relationships. This is where vision-language models excel, as they enable reasoning about images and their associated textual descriptions, allowing for a deeper understanding of the underlying semantics. Vision-language models can go beyond visual recognition of RS images, model semantic relationships, and generate natural language descriptions of the image. This makes them better suited for tasks requiring visual and textual understanding, such as image captioning, and visual question answering. This paper provides a comprehensive review of the research on vision-language models in remote sensing, summarizing the latest progress, highlighting challenges, and identifying potential research opportunities. Specifically, we review the application of vision-language models in mainstream remote sensing tasks, including image captioning, text-based image generation, text-based image retrieval, visual question answering, scene classification, semantic segmentation, and object detection. For each task, we analyze representative works and discuss research progress. We also summarize the commonly used remote sensing vision-language datasets, codebases, and online accessible resources. Finally, we delineate the limitations of current studies and suggest potential avenues for future advancements. This review aims to provide a comprehensive review of the current research pro

关键词： Semantics

Looking Beyond Two Frames: End-to-End Multi-Object Tracking Using Spatial and Temporal Transformers

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arXiv 2021年

作者： Zhu, Tianyu Hiller, Markus Ehsanpour, Mahsa Ma, Rongkai Drummond, Tom Reid, Ian Rezatofighi, Hamid The Department of Electrical and Computer Systems Engineering Monash University Australia The School of Computing and Information Systems The University of Melbourne Australia The Australian Institute for Machine Learning The University of Adelaide Australia The Department of Data Science and AI Monash University Australia The Australian Centre for Robotic Vision Australia

Tracking a time-varying indefinite number of objects in a video sequence over time remains a challenge despite recent advances in the field. Most existing approaches are not able to properly handle multi-object tracking challenges such as occlusion, in part because they ignore long-term temporal information. To address these shortcomings, we present MO3TR: a truly end-to-end Transformer-based online multi-object tracking (MOT) framework that learns to handle occlusions, track initiation and termination without the need for an explicit data association module or any heuristics. MO3TR encodes object interactions into long-term temporal embeddings using a combination of spatial and temporal Transformers, and recursively uses the information jointly with the input data to estimate the states of all tracked objects over time. The spatial attention mechanism enables our framework to learn implicit representations between all the objects and the objects to the measurements, while the temporal attention mechanism focuses on specific parts of past information, allowing our approach to resolve occlusions over multiple frames. Our experiments demonstrate the potential of this new approach, achieving results on par with or better than the current state-of-the-art on multiple MOT metrics for several popular multi-object tracking benchmarks. © 2021, CC BY.

关键词： Tracking (position)

Cultural Differences in Students' Privacy Concerns in learning Analytics across Germany, South Korea, Spain, Sweden, and the United States

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arXiv 2023年

作者： Viberg, Olga Kizilcec, René F. Jivet, Ioana Monés, Alejandra Martínez Oh, Alice Mutimukwe, Chantal Hrastinski, Stefan Scheffel, Maren School of Electrical Engineering and Computer Science KTH Royal Institute of Technology Lindstedsvägen 3 Stockholm10044 Sweden Department of Information Science Cornell University 107 Hoy Rd IthacaNY14853 United States DIPF Leibniz Institute for Research and Information in Education Rostocker Straße 6 Frankfurt am Main60323 Germany Scuela de Ingeniería Informática Valladolid University Paseo de Belén 15 Valladolid47011 Spain 291 Daehak-ro Yuseong-gu Daejeon34141 Korea Republic of Department of Computer and Systems Science Stockholm University NOD-huset Borgarfjordsgatan 12 Kist Stockholm164 55 Sweden Department of Learning and Engineering Sciences KTH Royal Institute of Technology Osquars backe 31 Stockholm10044 Sweden Institut für Erziehungswissenschaft Ruhr-Universität Bochum Universitätsstraße 150 BochumD - 44801 Germany

Applications of learning analytics (LA) can raise concerns from students about their privacy in higher education contexts. Developing effective privacy-enhancing practices requires a systematic understanding of students' privacy concerns and how they vary across national and cultural dimensions. We conducted a survey study with established instruments to measure privacy concerns and cultural values for university students in five countries (Germany, South Korea, Spain, Sweden, and the United States;N = 762). The results show that students generally trusted institutions with their data and disclosed information as they perceived the risks to be manageable even though they felt somewhat limited in their ability to control their privacy. Across the five countries, German and Swedish students stood out as the most trusting and least concerned, especially compared to US students who reported greater perceived risk and less control. Students in South Korea and Spain responded similarly on all five privacy dimensions (perceived privacy risk, perceived privacy control, privacy concerns, trusting beliefs, and non-self-disclosure behavior), despite their significant cultural differences. Culture measured at the individual level affected the antecedents and outcomes of privacy concerns. Perceived privacy risk and privacy control increase with power distance. Trusting beliefs increase with a desire for uncertainty avoidance and lower masculinity. Non-self-disclosure behaviors rise with power distance and masculinity, and decrease with more uncertainty avoidance. Thus, cultural values related to trust in institutions, social equality and risk-taking should be considered when developing privacy-enhancing practices and policies in higher education. © 2023, CC BY-NC-ND.

关键词： Students

C3BAM-XAI: Convolutional Block Attention Module Enhanced Explainable Artificial Intelligence-Based Parkinson’s Disease Stage Classification

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Cognitive Computation 2025年第3期17卷 1-19页

作者： Abbas, Muhammad John Khan, Muhammad Attique Hamza, Ameer Alsenan, Shrooq Rehman, Aleesha Baili, Jamel Zhang, Yudong Department of Computer Science HITEC University Taxila47080 Pakistan Center of Artificial Intelligence College of Computer Engineering and Science Prince Mohammad Bin Fahd University Al-Khobar Saudi Arabia Centre of Real Time Computer Systems Kaunas University of Technology Kaunas51368 Lithuania Information Systems Department College of Computer and Information Sciences Princess Nourah Bint Abdulrahman University P.O. Box 84428 Riyadh11671 Saudi Arabia Pak-Austria Fachhochschule Institute of Applied Sciences and Technology Haripur Mang Pakistan Department of Computer Engineering College of Computer Science King Khalid University Abha61413 Saudi Arabia Department of Informatics University of Leicester Leicester United Kingdom

Parkinson’s disease (PD) is a progressive neurodegenerative disorder that causes significant impairment in neurons, physiological structures, and behavior of people. However, these changes are very subtle in the early stages of PD, making diagnosis and treatment challenging. To overcome such challenges, we proposed a novel convolutional neural network (CNN) architecture called C3BAM-XAI that is based on the convolutional block attention module (CBAM) enhanced CNN and explainable artificial intelligence (XAI). In the proposed architecture, the data imbalance issue was resolved at the initial stage by employing augmentation techniques for the training data. After that, the proposed CNN architecture is designed with CBAM. The CBAM architecture is based on two sub-modules—the channel attention module and the spatial attention module. The input of the initial convolutional layer is passed to the channel attention module, which is further passed to the spatial attention module for more critical information extraction. For smooth training, hyperparameters are selected using Nadam Optimization (NO) instead of manual assignment. In the testing phase, features are extracted from the dense layer and passed to several neural network classifiers. In addition to this, the designed model is interpreted using the explainable AI technique to see the predicted label and insight decision features. The experimental process was performed on the publically available PD Kaggle dataset and obtained an improved accuracy of 93.33%. In addition, several ablation studies were conducted, and it is concluded that the CBAM not only improved accuracy but also made this model a reliable tool for clinical diagnosis, both in terms of accuracy and interpretability. The results thus open new avenues toward applying deep learning models practically for applications in the medical domain and early-stage detection of Parkinson’s disease, thereby helping to improve patient outcomes. © The Author(s), under

关键词： Convolutional neural networks