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Bifunctional nanoprobe for simultaneous detection of intracellular reactive oxygen species and temperature in single cells

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Microsystems & Nanoengineering 2024年第6期10卷 161-172页

作者： Yanmei Ma Weikang Hu Jian Hu Muyang Ruan Jie Hu Ming Yang Yi Zhang Hanhan Xie Chengzhi Hu Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems Department of Mechanical and Energy EngineeringSouthern University of Science and TechnologyShenzhen 518000China Department of Electrical and Electronic Engineering Southern University of Science and TechnologyShenzhen 518000China

Living cells can rapidly adjust their metabolic activities in response to external stimuli,leading to fluctuations in intracellular temperature and reactive oxygen species(ROS)*** these parameters is essential for understanding cellular metabolism,particularly during dynamic biological *** this study,we present a bifunctional nanoprobe capable of simultaneous measurement of ROS levels and temperature within single *** nanoprobe features two individually addressable nanoelectrodes,with platinum(Pt)and nickel(Ni)coatings on both *** the tip,these two metal layers form a nano-thermocouple,enabling precise intracellular temperature measurements,while the Pt layer facilitates selective ROS *** dual functionality allows for real-time monitoring of cellular responses during synergistic chemo-photothermal therapy of cancer cells and zebrafish embryos subjected to mitochondrial toxic *** results demonstrate that the nanoprobe effectively measures increases in temperature and ROS levels in HeLa cells undergoing chemo-photothermal therapy,as well as in chemically stimulated zebrafish *** providing detailed analysis of submicrometer-scale temperature and ROS variations within living cells,this nanoprobe offers valuable insights into cellular processes and holds promise for early disease detection and drug development.

关键词： metabolism simultaneous holds

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Aggressive and robust low-level control and trajectory tracking for quadrotors with deep reinforcement learning

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Neural Computing and Applications 2025年第3期37卷 1223-1240页

作者： Chen, Shiyu Li, Yanjie Lou, Yunjiang Lin, Ke Department of Control Science and Engineering Harbin Institute of Technology Shenzhen Shenzhen518055 China Guangdong Key Laboratory of Intelligent Morphing Mechanisms and Adaptive Robotics Shenzhen518055 China

Executing accurate trajectory tracking tasks using a high-performance low-level controller is crucial for quadrotors to be applied in various scenarios, especially those involving uncertain disturbances. However, due to the uncertainties in disturbed environments, developing effective low-level controllers with traditional model-based control schemes is challenging. This paper presents an aggressive and robust reinforcement learning (RL)-based low-level control policy for quadrotors. The policy maps the observed quadrotor state directly to motor thrust commands, without requiring the quadrotor dynamics. Additionally, a trajectory generation pipeline is developed to improve the accuracy of trajectory tracking tasks based on differential flatness. With the learned low-level control policy, extensive simulations and real-world experiments are implemented to validate the performance of the policy. The results indicate that our RL-based low-level control policy outperforms traditional proportional–integral–derivative (PID) control methods and related learning-based policies in terms of accuracy and robustness, particularly in environments with uncertain disturbances. Furthermore, the proposed RL-based control policy exhibits an aggressive response in trajectory tracking, even when the speed of the desired trajectory is increased to 6 m/s. Moreover, the learned policy demonstrates strong vibration suppression capabilities and enables the quadrotor to recover to a hovering state from random initial conditions with shorter response time. © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2024.

关键词： Deep reinforcement learning

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Time-Varying Formation Tracking control of Heterogeneous Multi-Agent Systems With Intermittent Communications and Directed Switching Networks

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IEEE/CAA Journal of Automatica Sinica 2025年第1期12卷 294-296页

作者： Yuhan Wang Zhuping Wang Hao Zhang Huaicheng Yan the Shanghai Research Institute for Intelligent Autonomous Systems Tongji University the National Key Laboratory of Autonomous Intelligent Unmanned Systems the Frontiers Science Center for Intelligent Autonomous System of Ministry of Education the College of Electronic and Information Engineering the Department of Control Science and Engineering Tongji University the Key Laboratory of Advanced Control and Optimization for Chemical Processes of Ministry of Education the School of Information Science and Engineering East China University of Science and Technology

Dear Editor,This letter is concerned with the problem of time-varying formation tracking for heterogeneous multi-agent systems(MASs) under directed switching networks. For this purpose, our first step is to present so... 详细信息

关键词： Switches Topology Observers Formation control Event detection Switched systems Multi-agent systems control theory Autonomous systems Stability

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A piezoresistive-based 3-axial MEMS tactile sensor and integrated surgical forceps for gastrointestinal endoscopic minimally invasive surgery

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Microsystems & Nanoengineering 2024年第5期10卷 71-83页

作者： Cheng Hou Huxin Gao Xiaoxiao Yang Guangming Xue Xiuli Zuo Yanqing Li Dongsheng Li Bo Lu Hongliang Ren Huicong Liu Lining Sun School of Mechanical and Electrical Engineering Jiangsu Provincial Key Laboratory of Advanced RoboticsSoochow UniversitySuzhouChina Faculty of Mechanical and Electrical Engineering Kunming University of Science and TechnologyJingming South RoadKunmingChina Yunnan Key Laboratory of Intelligent Control and Application KunmingChina Department of Electronic Engineering The Chinese University of Hong Kong(CUHK)Hong KongChina. Department of Gastroenterology Qilu HospitalShandong UniversityJinanShandongChina

In robotic-assisted surgery(RAS),traditional surgical instruments without sensing capability cannot perceive accurate operational forces during the task,and such drawbacks can be largely intensified when sophisticated tasks involving flexible and slender arms with small end-effectors,such as in gastrointestinal endoscopic surgery(GES).In this study,we propose a microelectromechanical system(MEMS)piezoresistive 3-axial tactile sensor for GES forceps,which can intuitively provide surgeons with online force feedback during robotic *** MEMS fabrication process facilitates sensor chips with miniaturized *** fully encapsulated tactile sensors can be effortlessly integrated into miniature GES forceps,which feature a slender diameter of just 3.5 mm and undergo meticulous calibration procedures via the least squares *** experiments,the sensor's ability to accurately measure directional forces up to 1.2 N in the Z axis was validated,demonstrating an average relative error of only 1.18%compared with the full-scale *** results indicate that this tactile sensor can provide effective 3-axial force sensing during surgical operations,such as grasping and pulling,and in ex vivo testing with a porcine *** compact size,high precision,and integrability of the sensor establish solid foundations for clinical application in the operating theater.

关键词： axial surgery intensified

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Adaptive Closed-Loop Functional Electrical Stimulation System With Visual Feedback for Enhanced Grasping in Neurological Impairments

IEEE Transactions on Medical Robotics and Bionics

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IEEE Transactions on Medical robotics and Bionics 2025年第2期7卷 678-686页

作者： Lin, Chengyu Cheng, Kong Hoi Pan, Wei Sun, Jinxin Gou, Guotao Fu, Junyun Leng, Yuquan Fu, Chenglong Southern University of Science and Technology Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems Guangdong Provincial Key Laboratory of Human-Augmentation Rehabilitation Robotics in Universities Department of Mechanical and Energy Engineering Shenzhen518055 China Guilin University of Electronic Technology Guangxi Human Physiological Information Non Invasive Detection Engineering Technology Research Center School of Life and Environmental Sciences Guilin541004 China

Grasping is a critical motor skill essential for daily activities, but it is often compromised in individuals with neural impairments. Functional Electrical Stimulation (FES) has emerged as a promising intervention, utilizing electrical pulses to stimulate muscles and thereby restore impaired motor functions. However, existing closed-loop FES systems depend on pre-calibrated angles or forces specific to individual objects, which limits their practicality in dynamic, real-world environments with varying object properties. This paper presents a novel closed-loop FES (CLFES) system with visual feedback, designed to dynamically adjust stimulation parameters based on real-time interaction states without requiring object-specific calibration. The system employs a finite state machine to manage sequential grasp-release tasks and integrates a visual perception module for slip detection and intent recognition. The system was tested with two individuals with disabilities on five common household objects. Experimental results demonstrate significant improvements, including a 42.6% increase in success rate and a 45.9% reduction in task completion time compared to tasks performed without the system. These results underscore the system's potential to improve daily task performance for individuals with neural impairments. © 2018 IEEE.

关键词： Visual servoing

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Real-time litchi detection in complex orchard environments: A portable, low-energy edge computing approach for enhanced automated harvesting

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Artificial Intelligence in Agriculture 2024年第1期11卷 13-22页

作者： Zeyu Jiao Kai Huang Qun Wang Zhenyu Zhong Yingjie Cai Guangdong Key Laboratory of Modern Control Technology Institute of Intelligent ManufacturingGuangdong Academy of SciencesGuangzhouChina School of Economics and Management Beihang UniversityBeijingChina Department of Electronic Engineering The Chinese University of Hong KongHong Kong

Litchi,a succulent and perishable fruit,presents a narrow annual harvest window of under two *** advent of smart agriculture has driven the adoption of visually-guided,automated litchi harvesting ***,conventional approaches typically rely on laboratory-based,high-performance computing equipment,which presents challenges in terms of size,energy consumption,and practical application within litchi *** address these limitations,we propose a real-time litchi detection methodology for complex environments,utilizing portable,low-energy edge computing ***,the litchi orchard imagery is collected to enhance data ***,a convolutional neural network(CNN)-based single-stage detector,YOLOx,is constructed to accurately pinpoint litchi fruit locations within the *** facilitate deployment on portable,low-energy edge devices,we employed channel pruning and layer pruning algorithms to compress the trained model,reducing its size and ***,the knowledge distillation technique is harnessed to fine-tune the *** findings demonstrated that our proposed method achieved a 97.1%compression rate,yielding a compact litchi detection model of a mere 6.9 MB,while maintaining 94.9%average precision and 97.2%average *** 99 frames per second(FPS),the method exhibited a 1.8-fold increase in speed compared to the unprocessed ***,our approach can be readily integrated into portable,low-computational automatic harvesting equipment,ensuring real-time,precise litchi detection within orchard settings.

关键词： Litchi detection Automated harvesting Edge computing Neural networks Model compression

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A practical PID variable stiffness control and its enhancement for compliant force-tracking interactions with unknown environments

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Science China(Technological Sciences) 2023年第10期66卷 2882-2896页

作者： ZHANG Xin ZHOU Hao LIU JinGuo JU ZhaoJie LENG YuQuan YANG ChenGuang State Key Laboratory of Robotics Shenyang Institute of AutomationChinese Academy of SciencesShenyang 110016China Institutes for Robotics and Intelligent Manufacturing Chinese Academy of SciencesShenyang 110169China University of Chinese Academy of Sciences Beijing 100049China School of Computing University of PortsmouthPortsmouth PO13HEUK Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems Department of Mechanical and Energy EngineeringSouthern University of Science and TechnologyShenzhen 518055China Key Laboratory of Autonomous Systems and Networked Control College of Automation Science and EngineeringSouth China University of TechnologyGuangzhou 510640China

Compliant interaction control is a key technology for robots performing contact-rich manipulation *** design of the compliant controller needs to consider the robot hardware because complex control algorithms may not be compatible with the hardware performance,especially for some industrial robots with low bandwidth *** paper focuses on effective and easy-to-use compliant control algorithms for position/velocity-controlled *** by human arm stiffness adaptation behavior,a novel variable target stiffness(NVTS)admittance control strategy is proposed for adaptive force tracking,in which a proportional integral derivative(PID)variable stiffness law is designed to update the stiffness coefficient of the admittance function by the force and position ***,its stability and force-tracking capability are theoretically *** addition,an impact compensator(Impc)is integrated into the NVTS controller to enhance its disturbance-suppression capability when the robot is subjected to strong vibration disturbances in complicated surface polishing *** proposed controllers are validated through four groups of experimental tests using different robots and the corresponding results demonstrate that they have high-accuracy tracking capability and strong adaptability in unknown environments.

关键词： compliant interaction control force tracking PID variable stiffness impact compensator unknown environments

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Collaborative Routing and Charging/Discharging Scheduling of Electric Autonomous Vehicles in Coupled Power-Traffic Networks: A Multi-Objective Approach

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IEEE Internet of Things Journal 2025年第11期12卷 17753-17764页

作者： Chu, Kai-Fung Chen, Tianlun Xie, Yue Lam, Albert Y.S. Song, Yue Iida, Fumiya University of Cambridge Department of Engineering CambridgeCB2 1PZ United Kingdom The University of Hong Kong Department of Electrical and Electronic Engineering Hong Kong The University of Hong Kong Department of Electrical and Electronic Engineering Department of Geography Hong Kong Fano Labs Hong Kong Tongji University Department of Control Science and Engineering Shanghai201804 China National Key Laboratory of Autonomous Intelligent Unmanned Systems Shanghai201210 China Ministry of Education Frontiers Science Center for Intelligent Autonomous Systems Shanghai200120 China

Autonomous vehicles (AVs) are vehicles that traverse on the road without active human intervention. With a coordinator, AVs can be connected to provide high-efficiency transport services, such as AV-based public transport networks. The controller can manage the network by coordinating the transport request assignment, traveling, and charging/discharging schedule. On the other hand, AVs are likely to be electric and benefit the smart grid via vehicle-to-grid technology. A well-designed mobility network connecting electric AVs (EAVs) and smart grid can substantially reduce unnecessary travel and energy costs. In this paper, we aim to maximize utilities in the AV-based public transport network and the power distribution network for the vehicle network containing EAVs, charging stations, and distributed power generations. We formulate the assignment and scheduling problem as a multi-objective mixed-integer program. To solve the optimization problem, we develop a hybrid heuristic approach based on Non-Dominated Sorting Genetic Algorithm II and branch-and-bound algorithms. Experiments are conducted on a modified 15-bus distribution system and a simulated traffic network. The results show that the proposed strategy effectively minimizes the total travel and energy purchase cost by 21%. This study provides valuable insights on vehicle coordination for multiple tasks, offering visionary guidance for stakeholders engaged in multifaceted transportation endeavors. © 2014 IEEE.

关键词： Vehicle-to-grid

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AIoT for Aircraft Final Assembly:An intelligent and Collaborative Framework

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Journal of Communications and Information Networks 2024年第3期9卷 262-276页

作者： Cheng Ren Xiaojing Wen Xinping Guan Cailian Chen Yehan Ma Department of Automation Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of System Control and Information Processing Ministry of Education of ChinaShanghai 200240China Shanghai Engineering Research Center of Intelligent Control and Management Shanghai 200240China School of Electronic Information and Electrical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Aircraft final assembly line(AFAL)involves thousands of processes that must be completed before ***,the heavy reliance on manual labor in most assembly processes affects the quality and prolongs the delivery *** the advent of artificial intelligence of things(AIoT)technologies has introduced advancements in certain AFAL scenarios,systematically enhancing the intelligence level of the AFAL and promoting the widespread deployment of artificial intelligence(AI)technologies remain significant *** address these challenges,we propose the intelligent and collaborative aircraft assembly(ICAA)framework,which integrates AI technologies within a cloud-edge-terminal *** ICAA framework is designed to support AI-enabled applications in the AFAL,with the goal of improving assembly efficiency at both individual and multiple process *** analyze specific demands across various assembly scenarios and introduce corresponding AI technologies to meet these *** three-tier ICAA framework consists of the assembly field,edge data platform,and assembly cloud platform,facilitating the collection of heterogeneous terminal data and the deployment of AI *** framework enhances assembly efficiency by reducing reliance on manual labor for individual processes and fostering collaboration across multiple *** provide detailed descriptions of how AI functions at each level of the ***,we apply the ICAA framework to a real AFAL,focusing explicitly on the flight control system testing *** practical implementation demonstrates the effectiveness of the framework in improving assembly efficiency and promoting the adoption of AIoT technologies.

关键词： intelligent aircraft final assembly line AIoT cloud-edge-terminal framework design

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Process Monitoring Based on Temporal Feature Agglomeration and Enhancement

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IEEE/CAA Journal of Automatica Sinica 2023年第3期10卷 825-827页

作者： Xiao Liang Weiwu Yan Yusun Fu Huihe Shao Department of Automation the School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Automation the School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240 Key Laboratory of System Control and Information Processing Ministry of Education of China Shanghai Engineering Research Center of Intelligent Control and Management Shanghai 200240China

Dear Editor,This letter proposes a process-monitoring method based on temporal feature agglomeration and enhancement,in which a novel feature extractor called contrastive feature extractor(CFE)extracts the temporal and relational features among process *** the feature representations are enhanced by maximizing the separation among different classes while minimizing the scatter within each class.

关键词： among while letter

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