检索结果-内蒙古大学图书馆

Application of Acinetobacter indicus to promote cigarette smoke particulate matter phytoremediation: removal efficiency and plant–microbe interactions

引用

Environmental Science and Pollution Research 2024年第39期31卷 52352-52370页

作者： Nookongbut, Phitthaya Thiravetyan, Paitip Salsabila, Salma Widiana, Ana Krobthong, Sucheewin Yingchutrakul, Yodying Treesubsuntorn, Chairat School of Bioresources and Technology King Mongkut’s University of Technology Thonburi Bangkok10150 Thailand Department of Biology Faculty of Science and Technology State Islamic University Sunan Gunung Djati Bandung West Java Bandung City40614 Indonesia Interdisciplinary Graduate Program in Genetic Engineering Kasetsart University Bangkok10900 Thailand Proteomics Research Team National Omics Center NSTDA Khlong Luang Pathum Thani12120 Thailand Pilot Plant Development and Training Institute King Mongkut’s University of Technology Thonburi Bangkok10150 Thailand

Particulate matter (PM) is one of the most hazardous atmospheric pollutants. Several plant species show high potential to reduce air pollutants and are widely used as green belts to provide clean outdoor spaces for human well-being. However, high PM concentrations cause physiological changes and stress in plants. In this study, 11 species of Thai native perennial plants were exposed to PM generated from tobacco smoke. Wrightia religiosa (Teijsm. & Binn.) Benth. ex Kurz, Bauhinia purpurea DC. ex Walp. and Tectona grandis L.f. reduced PM effectively (which is in the typical range of 43.95 to 52.97%) compared to other plant species. In addition, the responses of perennial plants under PM stress at the proteomic level were also evaluated. Proteomic analysis of these three plant species showed that plants respond negatively to high PM concentrations, such as reducing several photosynthetic-related proteins and increasing plant stress response proteins. To improve PM phytoremediation efficiency and reduce plant stress from PM, perennial plant–microbe interactions were investigated. W. religiosa was inoculated with Acinetobacter indicus PS1, and high biosurfactant-producing strains clearly showed a higher PM removal efficiency than non-inoculated plants (9.48, 9.5 and 12.6% for PM1.0, PM2.5 and PM10, respectively). Inoculating W. religiosa with A. indicus PS1 maintained chlorophyll a and b concentrations. Moreover, the malondialdehyde (MDA) concentration of W. religiosa inoculated with A. indicus PS1 was lower than that of non-inoculated W. religiosa. The leaf wax content (µg/cm2) and biosurfactant (µg/cm2) of W. religiosa inoculated with A. indicus PS1 were also higher than those of non-inoculated W. religiosa. This study clearly showed that inoculating plants with A. indicus PS1 can help plants remediate PM and improve their PM stress response. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.

关键词： Air pollution

来源：评论

学校读者我要写书评

暂无评论

Genome-wide analysis of detoxification genes conferring diamide insecticide resistance in Spodoptera exigua identifies CYP9A40

引用

Chemosphere 2024年 367卷 143623页

作者： Han, Changhee Rahman, Md-Mafizur Kim, Juil Lueke, Bettina Nauen, Ralf Interdisciplinary Graduate Program in Smart Agriculture Kangwon National University Chuncheon24341 Korea Republic of Department Biotechnology and Genetic Engineering Faculty of Biological Science Islamic University Kushtia7003 Bangladesh Agriculture and Life Science Research Institute Kangwon National University Chuncheon24341 Korea Republic of Bayer AG Crop Science Division R&D Monheim40789 Germany

For over a decade, diamide insecticides have been effective against lepidopteran pests like beet armyworm, Spodoptera exigua (Hübner, 1808). However, the evolution of resistance poses a challenge to their sustainable use. We identified an I4790 M mutation in the S. exigua ryanodine receptor (RyR) gene, but its correlation with resistance varied across the field-collected Korean populations of S. exigua. RNA sequencing and differential gene expression analysis were performed to investigate other resistance mechanisms. Diamide-resistant and susceptible strains and F1 hybrids were compared by mapping RNA-seq reads to the S. exigua reference genome. CYP9A40 was identified as a critical gene in diamide resistance due to its high expression in the resistant strains. Synergist bioassays with piperonyl butoxide supported the role of P450s in diamide metabolic resistance in S. exigua. A strong positive correlation between CYP9A40 over-expression levels (up to 80-fold) and diamide LC50 values was obtained for field-collected populations uniformly showing a 100% frequency of the RyR I4790 M target-site resistance allele. To validate the function of CYP9A40 in diamide detoxification, we recombinantly expressed the gene and tested its ability to bind and degrade chlorantraniliprole as a substrate. The results confirmed its catalytic role in diamide metabolism. CYP9A40 has been identified and validated to confer metabolic resistance in Korean S. exigua populations. It works alongside the RyR target-site I4790 M mutation to enhance diamide resistance. These mechanisms offer insights for resistance monitoring and support insecticide resistance management programs to improve control strategies for S. exigua. © 2024 The Author(s)

关键词： Spodoptera exigua Diamide P450 CYP9A40 Heterologous expression RNA-Seq

来源：评论

学校读者我要写书评

暂无评论

Genome-Wide Analysis of Detoxification Genes Conferring Diamide Insecticide Resistance in Spodoptera Exigua Identifies Cyp9a40

SSRN

引用

SSRN 2024年

作者： Kim, Juil Han, Changhee Rahman, Md-Mafizur Lueke, Bettina Nauen, Ralf Interdisciplinary Graduate Program in Smart Agriculture Kangwon National University Chuncheon24341 Korea Republic of Department Biotechnology and Genetic Engineering Faculty of Biological Science Islamic University Kushtia7003 Bangladesh Agriculture and Life Science Research Institute Kangwon National University Chuncheon24341 Korea Republic of Bayer AG Crop Science Division Monheim40789 Germany

For over a decade, diamide insecticides have been effective against lepidopteran pests like beet armyworm, Spodoptera exigua (Hübner, 1808). However, the evolution of resistance poses a challenge to their sustainable use. We identified an I4790M mutation in the S. exigua ryanodine receptor (RyR) gene, but its correlation with resistance varied across the field-collected Korean populations of S. exigua. RNA sequencing and differential gene expression analysis were performed to investigate other resistance mechanisms. Diamide-resistant and susceptible strains and F1 hybrids were compared by mapping RNA-seq reads to the S. exigua reference genome. CYP9A40 was identified as a critical gene in diamide resistance due to its high expression in the resistant strains. Synergist bioassays with piperonyl butoxide supported the role of P450s in diamide metabolic resistance in S. exigua. A strong positive correlation between CYP9A40 over-expression levels (up to 80-fold) and diamide LC50 values was obtained for field-collected populations uniformly showing a 100% frequency of the RyR I4790M target-site resistance allele. To validate the function of CYP9A40 in diamide detoxification, we recombinantly expressed the gene and tested its ability to bind and degrade chlorantraniliprole as a substrate. The results confirmed its catalytic role in diamide metabolism. CYP9A40 has been identified and validated to confer metabolic resistance in Korean S. exigua populations. It works alongside the RyR target-site I4790M mutation to enhance diamide resistance. These mechanisms offer insights for resistance monitoring and support insecticide resistance management programs to improve control strategies for S. exigua. © 2024, The Authors. All rights reserved.

关键词： RNA

来源：评论

学校读者我要写书评

暂无评论

Brassinosteroid Signalling and an Alternate Carbon Metabolism Pathway in the Particulate Matter and Volatile Organic Compound Stress Response of Sansevieria Trifasciata

SSRN

引用

SSRN 2022年

作者： Permana, Bayu Hadi Krobthong, Sucheewin Yingchutrakul, Yodying Saithong, Treenut Thiravetyan, Paitip Treesubsuntorn, Chairat School of Bioresources and Technology King Mongkut’s University of Technology Thonburi Bangkok10150 Thailand Interdisciplinary Graduate Program in Genetic Engineering Kasetsart University Bangkok10900 Thailand Proteomics Research Team National Omics Center NSTDA Pathum Thani12120 Thailand

Phytoremediation has become a popular technology due to its low cost, sustainability, and environmentally friendly use for removing particulate matter (PM) and volatile organic compound (VOC) pollution. However, under stress conditions, pollution is detrimental to plant growth and can even cause plant death. To provide better knowledge of plants’ response under PM and VOC stress, physiology and proteomics were used to explain a comprehensive mechanism of the stress response. In this study, Sansevieria trifasciata, a common air phytoremediation plant, was exposed to PM and VOC stress conditions generated from cigarette smoke in a 15 L chamber for six cycles, with the fifth cycle being a recovery period (30 days without smoke). Plant physiological stress responses, such as chlorophyll, carotenoid, and malondialdehyde (MDA) content, were examined. Exposure to PM and VOCs affected MDA content over time but not chlorophyll and carotenoid content. The exposure continuously caused plant stress, while the recovery period of 30 days relieved the stress. The proteomics results showed that plants under PM and VOCs activated brassinosteroid signalling, which might have enhanced the photosynthesis process and antioxidant system. The plant uses an alternate carbon metabolism pathway through the folate cycle to provide carbon sources for the synthesis of other metabolites. This study is the first proteomics approach to clearly show the response of proteins under PM and VOC stress. Understanding the plant stress response may enable a guideline to provide well-being conditions for phytoremediation. © 2022, The Authors. All rights reserved.

关键词： Volatile organic compounds

来源：评论

学校读者我要写书评

暂无评论

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites

引用

Cancer Communications 2024年第1期44卷 47-75页

作者： Ji Eon Kim So-Young Park Chulhwan Kwak Yoonji Lee Dae-Geun Song Jae Woo Jung Haesong Lee Eun-Ae Shin Yangie Pinanga Kyung-hee Pyo Eun Hae Lee Wonsik Kim Soyeon Kim Chang-Duck Jun Jeanho Yun Sun Choi Hyun-Woo Rhee Kwang-Hyeon Liu Jung Weon Lee Department of Pharmacy College of PharmacySeoul National UniversitySeoulRepublic of Korea Research Institute of Pharmaceutical Sciences College of PharmacySeoul National UniversitySeoulRepublic of Korea BK21 FOUR Community-Based Intelligent Novel Drug Discovery Education Unit College of Pharmacy and Research Institute of Pharmaceutical SciencesKyungpook National UniversityDaeguRepublic of Korea Department of Chemistry Seoul National UniversitySeoulRepublic of Korea College of Pharmacy Chung-Ang UniversitySeoulRepublic of Korea Natural Product Informatics Research Center Korea Institute of Science and Technology(KIST)Gangneung-siGangwon-doRepublic of Korea School of Life Sciences Gwangju Institute of Science and Technology(GIST)GwangjuRepublic of Korea Department of Biochemistry College of MedicineDong-A UniversityBusanRepublic of Korea Global AI Drug Discovery Center College of Pharmacy and Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoulRepublic of Korea Interdisciplinary Program in Genetic Engineering Seoul National UniversitySeoulRepublic of Korea

Background:Transmembrane 4 L six family member 5(TM4SF5)translocates subcellularly and functions metabolically,although it is unclear how intracellu-lar TM4SF5 translocation is linked to metabolic *** is thus of interests to understand how the traffic dynamics of TM4SF5 to subcellular endosomal membranes are correlated to regulatory roles of ***:Here,we explored the metabolic significance of TM4SF5 localization at mitochondria-lysosome contact sites(MLCSs),using in vitro cells and in vivo animal systems,via approaches by immunofluorescence,proximity labelling based proteomics analysis,organelle reconstitution ***:Upon extracellular glucose repletion following depletion,TM4SF5 became enriched at MLCSs via an interaction between mitochondrial FK506-binding protein 8(FKBP8)and lysosomal *** labeling showed molecular clustering of phospho-dynamic-related protein I(DRP1)and certain mitophagy receptors at TM4SF5-enriched MLCSs,leading to mitochondrial fis-sion and ***4SF5 bound NPC intracellular cholesterol transporter 1(NPC1)and free cholesterol,and mediated export of lysosomal cholesterol to mitochondria,leading to impaired oxidative phosphorylation but intact tri-carboxylic acid(TCA)cycle andβ-*** mouse models,hepatocyte Tm4sf5 promoted mitophagy and cholesterol transport to mitochondria,both with positive relations to liver ***:Our findings suggested that TM4SF5-enriched MLCSs regu-late glucose catabolism by facilitating cholesterol export for mitochondrial reprogramming,presumably while hepatocellular carcinogenesis,recapitulating aspects for hepatocellular carcinoma metabolism with mitochondrial repro-gramming to support biomolecule synthesis in addition to glycolytic energetics.

关键词： cholesterol fluorescent imaging glucose catabolism hepatocellular carcinogenesis mem-brane contact sites mitochondria function mitophagy oxidative phosphorylation protein-protein interaction tetraspanin

来源：评论

学校读者我要写书评

暂无评论

Bioluminescence-Activated Proximity Labeling for Spatial Multi-Omics

SSRN

引用

SSRN 2024年

作者： Wang, Ruixiang Fang, Yuxin Hu, Youyue Liu, Yanjun Chen, Peng R. Zou, Peng College of Chemistry and Molecular Engineering Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering Ministry of Education PKU-IDG/McGovern Institute for Brain Research Peking University Beijing100871 China Peking-Tsinghua-NIBS Joint Graduate Program Peking University Beijing100871 China National Key Laboratory of Crop Genetic Improvement Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan430070 China Academy for Advanced Interdisciplinary Studies PKU-Tsinghua Center for Life Science Peking University Beijing100871 China Beijing102206 China

Mapping the spatial organization of proteins and cellular interactions is crucial for understanding their biological functions. Herein, we report a biocompatible, multi-functional luminescence-activated proximity labeling (LAP) strategy for profiling subcellular proteomes and cell-cell interactions in live cells and animals. Our method capitalizes on fusing the photocatalyst miniSOG to NanoLuc luciferase, whose bioluminescence activates miniSOG via a resonance energy transfer mechanism, generating reactive oxygen species in situ to mediate proximity labeling. We demonstrated the high spatial specificity of LAP in a C57BL6/N mouse model transplanted with MC38 cells. Our data revealed tumor microenvironment-dependent remodeling of secretome. LAP was further applied to identify ligand-receptor mediated cell-cell interactions both in vitro and in vivo. We also achieved local transcriptome profiling by combining LAP with next-generation sequencing. Overall, LAP was proved to be a versatile PL technique with strong biocompatibility for spatial multi-omic applications. © 2024, The Authors. All rights reserved.

关键词： Bioluminescence

来源：评论

学校读者我要写书评

暂无评论

Serum proteomic approach to identifying differentially expressed proteins in effusive feline infectious peritonitis

引用

Scientific Reports 2025年第1期15卷 1-12页

作者： Wassamon Moyadee Jatuporn Rattanasrisomporn Sittiruk Roytrakul Janthima Jaresitthikunchai Narumon Phaonakrop Kiattawee Choowongkomon Sekkarin Ploypetch Natthasit Tansakul Amonpun Rattanasrisomporn Graduate Program in Animal Health and Biomedical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand Department of Companion Animal Clinical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Pathum Thani Thailand Department of Biochemistry Faculty of Science Kasetsart University Bangkok Thailand Department of Clinical Sciences and Public Health Faculty of Veterinary Science Mahidol University Nakhon Pathom Thailand Department of Pharmacology Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand Interdisciplinary of Genetic Engineering and Bioinformatics Graduate School Kasetsart University Bangkok Thailand

Feline infectious peritonitis (FIP) is a lethal, viral-induced immune-mediated disease that remains a challenge for diagnosis and treatment in cats. Proteomic profiling, which analyzes the protein content of biological samples, offers the potential to identify novel biomarkers that could improve the diagnosis and management of FIP. This study aims to assess the serum proteome and identify proteins that differentiate healthy cats from cats diagnosed with effusive FIP using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). A total of 30 cats diagnosed with effusive FIP and 27 clinically normal cats were enrolled. Twenty-three proteins were significantly (p< 0.01, ≥ fivefold change in abundance) differentially expressed between cats with effusive FIP and controls. Among these, the P2X purinoceptor, DNA topoisomerase, Notch receptor 2, and cadherin-17 were identified as key proteins of interest in cats with effusive FIP. Our findings suggest that these differentially expressed proteins could serve as potential diagnostic biomarkers and therapeutic targets for FIP. However, further studies are needed to validate these findings and explore their potential applications.

关键词： Biomarkers Cats Coronavirus Feline infectious peritonitis Proteomics Serum

来源：评论

学校读者我要写书评

暂无评论

Correction: Resistance QTLs controlling leaf and neck blast disease identified in a doubled haploid rice population

引用

Euphytica 2023年第7期219卷 1-1页

作者： Korinsak, Siripar Sriprakhon, Saengchai Sirithunya, Kanyanat Sriwongchai, Tanee Wongsaprom, Chanakarn Plabpla, Anucha Vanavichit, Apichart Toojinda, Theerayut National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Khlong Luang Thailand Faculty of Science and Agricultural Technology Rajamangala University of Technology Lanna Chiangmai Thailand Department of Agronomy Faculty of Agriculture Kasetsart University Bangkok Thailand Interdisciplinary Graduate Program in Genetic Engineering and Bioinformatics Kasetsart University Bangkok Thailand Department of Agronomy Faculty of Agriculture at Kamphaeng Saen Kasetsart University Nakhon Pathom Thailand Rice Science Center Kasetsart University Nakhon Pathom Thailand

来源：评论

学校读者我要写书评

暂无评论

New potent epitopes from Leptospira borgpetersenii for the stimulation of humoral and cell-mediated immune responses: Experimental and theoretical studies

引用

Informatics in Medicine Unlocked 2021年 25卷

作者： Tansiri, Yada Sritrakul, Tepyuda Saparpakorn, Patchreenart Boondamnern, Timporn Chimprasit, Aunlika Sripattanakul, Sineenat Hannongbua, Supa Prapong, Siriwan Center for Advanced Studies for Agriculture and Food (CASAF) Kasetsart University Bangkok 10900 Thailand Faculty of Medicine King Mongkut's Institute of Technology Ladkrabang Bangkok 10520 Thailand Faculty of Veterinary Medicine Kasetsart University Kamphaeng Sean Campus Nakhon Pathom 73140 Thailand Department of Chemistry Faculty of Science Kasetsart University Bangkok 10900 Thailand Department of Physiology Faculty of Veterinary Medicine Kasetsart University Bangkok 10900 Thailand The Interdisciplinary Graduate Program in Genetic Engineering The Graduate School Kasetsart University Bangkok 10900 Thailand Akkhraratchakumari Veterinary College Walailak University. 222 Thaiburi Thasala District Nakhon Si Thammarat 80160 Thailand

The rhKU_Sej_LRR_2271 protein is introduced as one of the leptospiral vaccine candidates since it contains the predicted immunogenic epitopes. The in-silico sequence and structural based analysis have been used in this study to analyze MHC class I and class II-restricted epitopes of the protein. Six epitope prediction programs were employed, which the epitopes with high prediction scores from each program were aligned. The 21 of potentially unrestrained epitopes with prediction scores above the cut-off value from at least two prediction programs were selected. The 3D-molecular modeling, docking, and molecular dynamic simulation were performed to evaluate the affinity binding between peptide-MHC complex and T-cell receptor. One promising epitope, which is 171-LLFLPLIKI, showed the potency in binding to both MHC class I and II alleles. Two newly designed peptides containing epitopes which can bind to over 3 of MHC alleles, LL17:171-LLFLPLIKILYVDRNKL-187 and SL19:209-SLNSGIKALPFNYEKLVNL-227, significantly increased interferon-gamma (IFNg)-producing specific T-cell responses in the rhKU_Sej_LRR_2271 immunized rabbits compared to nonimmunized rabbits. The LL17 peptide can also induce interferon-gamma-producing specific CD4⁺ T-cell responses in the immunized rabbits. For the evaluation of humoral immune responses, the immunized rabbits have a significantly greater amount of specific IgG in plasma than the nonimmunized rabbits. Ex-vivo study of T-cell responses in animal model using flow cytometry confirmed an accomplishment of the theoretically in-silico analysis for discovering potential T-cell epitopes of the protein. The results show that the rhKU_Sej_LRR_2271 protein containing promiscuous T-cell epitopes, which can induce both humoral and cell-mediated immune responses, is a prospective protein candidate for leptospiral vaccine development. © 2021 The Authors

关键词： Epitopes Leptospiral vaccine MHC Molecular docking Molecular dynamic simulation T-cell receptor (TCR)

来源：评论

学校读者我要写书评

暂无评论

Potential anti-biofilm producing marine actinomycetes isolated from sea sediments in Thailand

引用

Agriculture and Natural Resources 2018年第3期52卷 228-233页

作者： Leetanasaksakul, Kantinan Thamchaipenet, Arinthip Department of Genetics Faculty of Science Kasetsart University Bangkok 10900 Thailand Interdisciplinary Graduate Program in Genetic Engineering Graduate School Kasetsart University Bangkok 10900 Thailand Center for Advanced Studies in Tropical Natural Resources Kasetsart University Bangkok 10900 Thailand

Marine actinomycetes are well known as novel resources of bioactive compounds including anti-biofilm agents with various therapeutic applications. In this study, one hundred and one marine actinomycetes were isolated from sea sediments collected from Andaman sea and the Gulf of Thailand. Morphological study and partial 16S rDNA sequence analysis revealed that most of isolates belong to genus Streptomyces (n = 90, 89.11%) and the rest are rare actinomycetes (n = 11, 10.89%) including Actinopolymorpha, Actinomycetospora, Dietzia Nocardiopsis, Micromonospora and Mycobacterium. Among them, ten and thirteen strains significantly reduced more than 60% of biofilm formation of Escherichia coli and Staphylococcus aureus, respectively. But no such activity was found against Pseudomonas aeruginosa. Further analysis of their supernatants indicated that most of marine actinomycetes secreted non-toxic anti-biofilm agents and some accompanied with protease activity. These are preferred characteristics of anti-biofilm substances that potentially restricted biofilm forming of E. coli and S. aureus and simultaneously prevented bacteria to develop resistance. © 2018 Kasetsart University

关键词： Anti-biofilm Marine actinomycetes

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：