We hypothesize that periodically early, local suppression of cysteinyl leukotrienes (CysLTs), which are potent inflammatory mediators, can reduce the fibrotic capsular contracture around silicone implants. We tested t...
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We hypothesize that periodically early, local suppression of cysteinyl leukotrienes (CysLTs), which are potent inflammatory mediators, can reduce the fibrotic capsular contracture around silicone implants. We tested this hypothesis with the silicone implants enabled with the sustained release of montelukast, a CysLT receptor antagonist, for 3 and 15 days. In this work, we inserted each of the distinct implants into the pocket of the subpanniculus carnosus plane of living rats and performed histological and immunofluorescent (IF) analyses of the tissues biopsied at predetermined periods for 12 weeks after implant insertion. The implants with montelukast exhibited significantly reduced polymorphonuclear leukocytes (i.e., PMNs), implying a concurrent reduction of CysLT. This effect was more prominent after long-term local montelukast exposure. Thus, fewer fibroblasts were recruited, thereby reducing transforming growth factor (TGF)-I3 and myofibroblasts in the tissue around the implant. Therefore, the fibrotic capsule formation, which was assessed using the capsule thickness and collagen density, decreased along with the myofibroblasts. Additionally, the tissue biopsied at the experimental end point exhibited significantly decreased mechanical stiffness. Statement of Significance Capsular contracture is troublesome, making the tissues hardened around the silicone implant. This causes serious pain and discomfort to the patients, often leading to secondary surgery for implant replacement. To resolve this, we suggest a strategy of long-term, local suppression of cysteinyl leukotriene, an important mediator present during inflammation. For this, we propose a silicone implant abled to release a drug, montelukast, in a sustained manner. We tested our drug -release implant in living animals, which exhibited a significant decrease in capsule formation compared with the intact silicone implant. Therefore, we conclude that the sustained release of montelukast at the local insert
Many animals alter their development according to environmental changes. However, how temporal information is used for large-timescale developmental decisions is relatively unknown due to the difficulty in precise tem...
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ISBN:
(纸本)9780979806490
Many animals alter their development according to environmental changes. However, how temporal information is used for large-timescale developmental decisions is relatively unknown due to the difficulty in precise temporal environmental control for long developmental experiments. In this paper, we present an experimental platform capable of long-term large population culture of developing C. elegans while achieving spatial consistency and temporal environmental control of a variety of stimuli. In addition, we were also able to quantify the divergence of a population's behavioral and morphological developmental response to pheromone stimulation over several days.
In this study,Cold adapted protease gene from psychrophilic bacteria Enterobacter *** 25617 was cloned,purified and was *** protease was expression using *** expression *** recombinant strain expressed the protease fr...
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In this study,Cold adapted protease gene from psychrophilic bacteria Enterobacter *** 25617 was cloned,purified and was *** protease was expression using *** expression *** recombinant strain expressed the protease from Enterobacter *** 25617 on inclusion body and expressed protease size is 67 ***
In this study,production,purification,characterization,and gene cloning of an extracellular peptiase from a psychrophilic Janthinobacterium lividum PAMC 25641 strain were *** peptidase was purified to homogeneity by 8...
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In this study,production,purification,characterization,and gene cloning of an extracellular peptiase from a psychrophilic Janthinobacterium lividum PAMC 25641 strain were *** peptidase was purified to homogeneity by 80%ammonium sulphate precipitation,anion-exchange chromatography,with a 3.48-fold increase in specific *** purified peptidase with a molecular mass of about 106 kDa exhibited optimal activity at pH 7.5
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