A sophisticatedly designed polyelectrolyte diode was fabricated on a microchip and exhibited well-defined nonlinear rectifying behavior. Multiple polyelectrolyte diodes were integrated on a microchip to produce a vari...
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This work reports a new method to partially coat a film of our interest on a microbead with a thin gold shell, briefly 'gold ball', using wireless electrochemistry. The key point of this method is to control t...
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This paper reports the decoding strategy of surface-enhanced Raman scattering (SERS) signals from microspheres with Au shell structures, or gold balls, with Raman-tags in a microfluidic channel. On the surface of the ...
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An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent mi...
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An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent micro- and nanospheres each conjugated with different proteins are size-dependently self-assembled (SDSA) onto these nanometer wells that were created on the polymethyl methacrylate (PMMA) substrate by electron beam lithography (EBL). Particles of different diameters are added serially, and electrostatically attached to the corresponding wells through electrostatic attraction between the carboxylic groups of the spheres and p-doped silicon substrate underneath the PMMA layer. Target detection utilizes fluorescence resonance energy transfer (FRET) from fluorescent beads to target (mouse immunoglobulin G = mIgG or Octamer-4 = Oct4) and its antibody bound on the beads. The 180 nm blue beads are conjugated with mIgG to capture anti-mIgG-FITC. The 50 nm green and 100 nm yellow-green beads are conjugated with anti-Oct4 to capture Oct4 peptides; where the secondary anti-Oct4 tagged with phycoerythrin via F(ab)2 fragment is then added to function as an indicator of Oct4 detection. These protein-conjugated particles are added serially from the largest to the smallest and the particles are successfully self-assembled to the respective nanometer wells to achieve size-dependent self-assembly. FRET signals are detected through fluorescence and confocal microscopes, and further confirmed by Fluorolog3 spectrofluorometer. Therefore, SDSA is a valuable approach for the fabrication of multiple components array; and FRET is a useful biorecognition technique for the detection of mIgG, Oct4 or other targets of interest.
A portable flow cytometer with simultaneous detection of impedance and fluorescence was constructed incorporating polyelectrolytic gel electrodes (PGE) based impedance measurement and fluorescence measurement with int...
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Authentication using finger vein pattern has substantial advantage than other biometrics. Because human vein patterns are hidden inside the skin and tissue, it is hard to forge vein structure. But conventional system ...
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This paper presents a natural-filling wireless pressure-flow study (NFW-PFS) system for the diagnosis of urologic problems which many patients in older population suffer from. For the benign prostatic hyperplasia (BPH...
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With the increase of the number of the blind, demand for visual prosthesis is increasing. Text reading is one of the most important visual functions to the blind. Therefore human trials and simulations of prosthetic v...
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With the increase of the number of the blind, demand for visual prosthesis is increasing. Text reading is one of the most important visual functions to the blind. Therefore human trials and simulations of prosthetic vision have already been carried out for testing an improved system or studying rehabilitation of people who implant prosthesis. But although forms of electrode arrays used in those studies were various, differences between performances of them were not considered for the text reading. Therefore we tested reading performances among three different array shapes (square (32×32), vertical(42×22) and horizontal(22×42) shape array) by blind simulation experiment with head mount display device. As a result, horizontal array showed the best performance when window length for horizontal array was 3. However, when the window width for horizontal array increased to 3.5, performances between horizontal array and square array were similar. In the experiment of lower resolution (8×8) and window width, square array showed the best performance than other arrays.
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