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13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Han, Ji-Hyung Kim, Kwang Bok Kim, Hee Chan Chung, Taek Dong Department of Chemistry Seoul National University Korea Republic of Interdisciplinary Program Bioengineering Major Graduate School Seoul National University Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Korea Republic of

ISBN: (纸本)9780979806421

A sophisticatedly designed polyelectrolyte diode was fabricated on a microchip and exhibited well-defined nonlinear rectifying behavior. Multiple polyelectrolyte diodes were integrated on a microchip to produce a variety of logic gates based on ionic circuits. By employing a fluorescent dye, the polyelectrolyte micro plugs also acted as optical indicators displaying the input signals applied to individual diodes in the ionic circuit on a microchip. Also, this system visualized the dynamic distribution of ions in a charged polymer phase under an electric field on a real time basis through fluorescence images. © 2009 CBMS.

关键词： Polyelectrolytes

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Janus micro gold shells fabricated by wireless electrochemistry

Janus micro gold shells fabricated by wireless electrochemis...

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13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Jo, Ji-Hum Noh, Jongmin Kim, Hee Chan Chung, Taek Dong Department of Chemistry Seoul National University Seoul Korea Republic of Interdisciplinary Program Graduate School Seoul National University Seoul Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Seoul Korea Republic of

ISBN: (纸本)9780979806421

This work reports a new method to partially coat a film of our interest on a microbead with a thin gold shell, briefly 'gold ball', using wireless electrochemistry. The key point of this method is to control the potential of the external electrodes, which will drive an electrochemical reaction on the gold ball in a microchannel without any electric contact. This method allows partial modification of a variety of organic or inorganic compounds that can electrochemical reaction. © 2009 CBMS.

关键词： Surface treatment

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SERS decoding using functionalized micro gold balls flowing in a microfluidic channel

SERS decoding using functionalized micro gold balls flowing ...

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13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Lee, Sa Ram Kim, Hee Chan Chung, Taek Dong Interdisciplinary Program Graduate School Seoul National University Seoul Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Seoul Korea Republic of Department of Chemistry Seoul National University Seoul Korea Republic of

ISBN: (纸本)9780979806421

This paper reports the decoding strategy of surface-enhanced Raman scattering (SERS) signals from microspheres with Au shell structures, or gold balls, with Raman-tags in a microfluidic channel. On the surface of the gold balls, Raman tags were functionalized. We measured SERS signals from functionalized gold balls on a glass plate and in a fluidic channel at static and flowing conditions, respectively. © 2009 CBMS.

关键词： Microfluidics

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Fluorescence resonance energy transfer detection of mouse immunoglobulin G and Octamer-4 on protein nanoarray

Fluorescence resonance energy transfer detection of mouse im...

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IEEE/ICME International Conference on Complex Medical engineering, CME

作者： Phat. L. Tran Yee Tchao Jeong-Yeol Yoon Biomedical Engineering Graduate Interdisciplinary Program University of Arizona Tucson Tucson AZ USA Department of Veterinary Science and Microbiology University of Arizona Tucson Tucson AZ USA Department of Agricultural and Biosystems Engineering and Biomedical Engineering Graduate Interdisciplinary Program University of Arizona Tucson Tucson AZ USA

An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent micro- and nanospheres each conjugated with different proteins are size-dependently self-assembled (SDSA) onto these nanometer wells that were created on the polymethyl methacrylate (PMMA) substrate by electron beam lithography (EBL). Particles of different diameters are added serially, and electrostatically attached to the corresponding wells through electrostatic attraction between the carboxylic groups of the spheres and p-doped silicon substrate underneath the PMMA layer. Target detection utilizes fluorescence resonance energy transfer (FRET) from fluorescent beads to target (mouse immunoglobulin G = mIgG or Octamer-4 = Oct4) and its antibody bound on the beads. The 180 nm blue beads are conjugated with mIgG to capture anti-mIgG-FITC. The 50 nm green and 100 nm yellow-green beads are conjugated with anti-Oct4 to capture Oct4 peptides; where the secondary anti-Oct4 tagged with phycoerythrin via F(ab)2 fragment is then added to function as an indicator of Oct4 detection. These protein-conjugated particles are added serially from the largest to the smallest and the particles are successfully self-assembled to the respective nanometer wells to achieve size-dependent self-assembly. FRET signals are detected through fluorescence and confocal microscopes, and further confirmed by Fluorolog3 spectrofluorometer. Therefore, SDSA is a valuable approach for the fabrication of multiple components array; and FRET is a useful biorecognition technique for the detection of mIgG, Oct4 or other targets of interest.

关键词： Fluorescence Resonance Energy exchange Mice Proteins Self-assembly Immune system Electron beams Lithography Electrostatics

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Quantifying the microstructure of human sclera using small angle light scattering

Quantifying the microstructure of human sclera using small a...

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10th ASME Summer Bioengineering Conference, SBC2008

作者： Eskinazi, Amanda E. Vande Geest, Jonathan P. Department of Biosystems Engineering University of Arizona Tucson AZ United States Department of Aerospace and Mechanical Engineering University of Arizona Tucson AZ United States Biomedical Engineering Graduate Interdisciplinary Program University of Arizona Tucson AZ United States BIO5 Institute University of Arizona Tucson AZ United States

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A portable flow cytometer with simultaneous detection of impedance and fluorescence

A portable flow cytometer with simultaneous detection of imp...

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13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Kim, Kee Hyun Joo, Segyeong Noh, Jong Min Chung, Taek Dong Kim, Hee Chan Interdisciplinary Program Graduate School Seoul National University Korea Republic of Institute of Medical and Biological Engineering Medical Research Center Seoul National University Korea Republic of Department of Chemistry Seoul National University Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Korea Republic of

ISBN: (纸本)9780979806421

A portable flow cytometer with simultaneous detection of impedance and fluorescence was constructed incorporating polyelectrolytic gel electrodes (PGE) based impedance measurement and fluorescence measurement with integrated solid-state photonic devices. Simultaneous detection of fluorescence and impedance signal was performed with fluorescent and plain beads. The fluorescence and impedance signal were in good sync with flow of beads. © 2009 CBMS.

关键词： Fluorescence

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Non-contact finger vein acquisition system using NIR laser

Non-contact finger vein acquisition system using NIR laser

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Sensors, Cameras, and Systems for Industrial/Scientific Applications X

作者： Kim, Jiman Kong, Hyoun-Joong Park, Sangyun Noh, SeungWoo Lee, Seung-Rae Kim, Taejeong Kim, Hee Chan Interdisciplinary Program Graduate School Seoul National University Seoul Korea Republic of Department of Electrical Engineering and Computer Sciences Seoul National University Seoul Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Seoul Korea Republic of Institute of Medical and Biological Engineering Medical Research Center Seoul National University Seoul Korea Republic of

Authentication using finger vein pattern has substantial advantage than other biometrics. Because human vein patterns are hidden inside the skin and tissue, it is hard to forge vein structure. But conventional system using NIR LED array has two drawbacks. First, direct contact with LED array raise sanitary problem. Second, because of discreteness of LEDs, non-uniform illumination exists. We propose non-contact finger vein acquisition system using NIR laser and Laser line generator lens. Laser line generator lens makes evenly distributed line laser from focused laser light. Line laser is aimed on the finger longitudinally. NIR camera was used for image acquisition. 200 index finger vein images from 20 candidates are collected. Same finger vein pattern extraction algorithm was used to evaluate two sets of images. Acquired images from proposed non-contact system do not show any non-uniform illumination in contrary with conventional system. Also results of matching are comparable to conventional system. We developed Non-contact finger vein acquisition system. It can prevent potential cross contamination of skin diseases. Also the system can produce uniformly illuminated images unlike conventional system. With the benefit of non-contact, proposed system shows almost equivalent performance compared with conventional system. © 2009 SPIE-IS&T.

关键词： Biometrics

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Development of wireless urodynamic pressure-flow study system

Development of wireless urodynamic pressure-flow study syste...

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World Congress on Medical Physics and biomedical engineering: Diagnostic and Therapeutic Instrumentation, Clinical engineering

作者： Kong, Hyoun-Joong Park, Sunmee Oh, Seung June Kim, Hee Chan Interdisciplinary Program Graduate School Seoul National University Seoul Korea Republic of Department of Urology College of Medicine Seoul National University Seoul Korea Republic of College of Medicine Seoul National University Department of Biomedical Engineering 101 Daehang-Ro Jongno-Gu Seoul Korea Republic of

ISBN: (纸本)9783642038846

This paper presents a natural-filling wireless pressure-flow study (NFW-PFS) system for the diagnosis of urologic problems which many patients in older population suffer from. For the benign prostatic hyperplasia (BPH) patients, NFW-PFS was performed in a private room. Several features were extracted from the pressure and flow data and used to make the artificial neural network classifier which was able to predict the status of BPH patient's bladder outlet. Results showed good agreement with theoretical predictions based on clinical evidence and significant improvement over conventional urodynamic study (UDS) system in terms of several subjective parameters. The work presented here has profound implications for future studies of wearable UDS system and may one day help solve the problem of urodynamic application to home healthcare. © 2009 Springer-Verlag.

关键词： Diagnosis

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A comparison of text reading speed using square and rectangular arrays for visual prosthesis

A comparison of text reading speed using square and rectangu...

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IEEE EMBS International Conference on Information Technology Applications in Biomedicine (ITAB)

作者： Min Hye Chang Hyun Seok Kim Jae Hyuk Shin Kwang Suk Park Interdisciplinary Program of Bioengineering Graduate School Seoul National University South Korea Department of Biomedical Engineering College of Medicine Seoul National University South Korea

With the increase of the number of the blind, demand for visual prosthesis is increasing. Text reading is one of the most important visual functions to the blind. Therefore human trials and simulations of prosthetic vision have already been carried out for testing an improved system or studying rehabilitation of people who implant prosthesis. But although forms of electrode arrays used in those studies were various, differences between performances of them were not considered for the text reading. Therefore we tested reading performances among three different array shapes (square (32×32), vertical(42×22) and horizontal(22×42) shape array) by blind simulation experiment with head mount display device. As a result, horizontal array showed the best performance when window length for horizontal array was 3. However, when the window width for horizontal array increased to 3.5, performances between horizontal array and square array were similar. In the experiment of lower resolution (8×8) and window width, square array showed the best performance than other arrays.

关键词： Visual prosthesis Testing Pixel Shape Image resolution Image processing biomedical engineering Prosthetics Implants Optical refraction

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REVIEW ARTICLE: Melatonin plus exercise-based neurorehabilitative therapy for spinal cord injury

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Journal of Pineal Research 2010年第3期49卷

作者： Yonggeun Hong K. J. Palaksha Kanghui Park Sookyoung Park Hyun-Dong Kim Russel J. Reiter Kyu-Tae Chang Cardiovascular & Metabolic Disease Center Department of Rehabilitation Science in Interdisciplinary PhD Program College of Biomedical Science & Engineering Inje University Gimhae National Primate Research Center (NPRC) Korea Research Institute of Bioscience and Biotechnology (KRIBB) Ochang Department of PM & R. (Physical Medicine and Rehabilitation) College of Medicine Inje University Busan Korea Department of Cellular & Structural Biology The UT Health Science Center San Antonio TX USA

Abstract: Spinal cord injury (SCI) is damage to the spinal cord caused by the trauma or disease that results in compromised or loss of body function. Subsequent to SCI in humans, many individuals have residual motor and sensory deficits that impair functional performance and quality of life. The available treatments for SCI are rehabilitation therapy, activity-based therapies, and pharmacological treatment using antioxidants and their agonists. Among pharmacological treatments, the most efficient and commonly used antioxidant for experimental SCI treatment is melatonin, an indolamine secreted by pineal gland at night. Melatonin’s receptor-independent free radical scavenging action and its broad-spectrum antioxidant activity makes it an ideal antioxidant to protect tissue from oxidative stress-induced secondary damage after SCI. Owing to the limitations of an activity-based therapy and antioxidant treatment singly on the functional recovery and oxidative stress-induced secondary damages after SCI, a melatonin plus exercise treatment may be a more effective therapy for SCI. As suggested herein, supplementation with melatonin in conjunction with exercise not only would improve the functional recovery by enhancing the beneficial effects of exercise but would reduce the secondary tissue damage simultaneously. Finally, melatonin may protect against exercise-induced fatigue and impairments. In this review, based on the documented evidence regarding the beneficial effects of melatonin, activity-based therapy and the combination of both on functional recovery, as well as reduction of secondary damage caused by oxidative stress after SCI, we suggest the melatonin combined with exercise would be a novel neurorehabilitative strategy for the faster recovery after SCI.

关键词： animal model exercise melatonin rehabilitation secondary/oxidative damage spinal cord injury

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