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25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Kim, You-Jeong Lee, Ha-Sung Chung, Aram Department of Chemical and Biological Engineering Sookmyung Women's University Seoul Korea Republic of School of Biomedical Engineering Korea University Seoul Korea Republic of Interdisciplinary Program in Precision Public Health Korea University Korea Republic of

ISBN: (纸本)9781733419031

A novel droplet-based intracellular delivery platform is presented leveraging droplet microfluidics with mechanical cell permeabilization. A single cell and cargos were encapsulated in a droplet using a flow focusing device. Then, the droplet was accelerated by the sheath flow and passed through a single constriction at high speed, creating membrane discontinuities that facilitate the convective transport of biomolecules into the cell. Using this principle, the cell mechanoporation was achieved in a controlled and reproducible manner, allowing highly efficient, and dosage-controllable with substantially lower analyte amounts. The approach also enables vector-free delivery while maintaining high cell integrity without channel clogging. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Cell engineering

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Registration of finger vein image using skin surface information for authentication

Registration of finger vein image using skin surface informa...

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Image Processing: Machine Vision Applications II

作者： Noh, SeungWoo Kong, Hyoun-Joong Park, SangYun Kim, JiMan Lee, Seung-Rae Kim, Taejeong Kim, Hee Chan Interdisciplinary Program Bioengineering Major Graduate School Seoul National University Seoul Korea Republic of Interdisciplinary Program Biomedical Engineering Major Graduate School Seoul National University Seoul Korea Republic of Institute of Medical and Biological Engineering Medical Research Center Seoul National University Seoul Korea Republic of Department of Electrical Engineering and Computer Sciences Seoul National University Seoul Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Seoul Korea Republic of

ISBN: (纸本)9780819475015

The finger vein image acquired with an acquisition system should be properly aligned to proceed with comparing algorithm. However it is not easy to find control the points since the images are naturally blurred with an inherent scattering property. To overcome this problem, we propose a novel finger vein registration method utilizing skin surface information (i.e. wrinkles and outlines). We assumed that finger crooking was insignificant. Images were sampled with intended translation and rotation. Each time, two images were acquired successively by switching the light source;one with infrared light and the other with white light. Degree of rotation and translation of sampled image were calculated using outline features in the white light image and then the infrared image was transformed according to the calculated data. To validate our method, correlation values were computed between identical subjects and different subjects. High correlation values were shown between identical subjects whereas low values were shown between different subjects. © 2009 SPIE.

关键词： Authentication

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The smoking acute effect on the cyclic variation in blood echogenicity fromthe common carotid artery

The smoking acute effect on the cyclic variation in blood ec...

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IEEE International Ultrasonics Symposium

作者： Li, Ying Bok, Tae-Hoon Yang, Jeong-Hwa Choi, Min-Joo Paeng, Dong-Guk Department of Oceanic Information and System Engineering Korea Republic of Interdisciplinary Postgraduate Program in Biomedical Engineering Korea Republic of Department of Medicine Jeju National University Korea Republic of Department of Radiotechnology Cheju Halla College Korea Republic of

ISBN: (纸本)9781457703829

The cyclic variation in echogenicity (CVE) was observed from thecross-sectional images of the common carotid artery using coded harmonic imagingof a GE ultrasound system. The objective of this research is to study thesmoking acute effects on the amplitude of the CVE (Acve). A cve was analyzed among the 28 smokers before and after 1-2 cigaretteswere smoked. The smokers were optimally divided into two clusters with respectto the change in Acve and the intrinsic characteristics of smokers(i.e., daily consumed cigarettes and smoking years) through a two-step clusteranalysis (TSCA). The relative change of Acve after smoking wassignificantly higher in the heavy smoker cluster compared with the light smokercluster. However, there is no significant difference in the absolute value ofAcve between before and after smoking for both two clusters. Theeffect of tissue attenuation on Acve was compensated with body massindex (BMI). The compensated parameter, A'cve, is significantlyincreased in heavy smoker group after smoking (BS: 0.477.4;AS: 5.48.0;p0.05).It is in agreement with the analytical result of AA cve. Furthermore,after smoking, A'cve is significant higher in heavy smoker group(5.48.0), compared with light smoker group (1.36.7;p0.05). It suggests that thecompensation of Acve is help to reduce the interference from tissueattenuation and is good for the comparison among individuals. © 2010 IEEE.

关键词： Health

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Effect of mouth-opening levels on sound field gain in the ear canal 45

Effect of mouth-opening levels on sound field gain in the ea...

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45th International Congress and Exposition on Noise Control engineering: Towards a Quieter Future, INTER-NOISE 2016

作者： Xiao, Jimg-Hong Yu, Jen-Fang Lee, Kun-Che Chiu, Hsiu-Hui Graduate Institute of Medical Mechatronics Chang Gung University Taiwan Taiouan Interdisciplinary Otolaryngology Laboratory Chang Gung University Taiwan Ph.D. Program in Biomedical Engineering College of Engineering Chang Gung University Taiwan

The human ear canal can become deformed when the mouth opens and closes, changing the sound field in the external auditory canal. Thus, measuring the mandibular movement trajectory may provide a reference for identifying mouth-opening levels. The human ear canal can become deformed when the mouth opens and closes, changing the sound field in the external auditory canal. Thus, measuring the mandibular movement trajectory may provide a reference for identifying mouth-opening levels. The K7 system and a Frye Fonix 7000 sensor were used to perform several measurements of mouth-opening and -closing to obtain the mean maximal mouth opening level. The mandible was fixed at the 1/3, 2/3, and maximal mouth-opening levels. Subsequently, a 65-dB sweep tone, which is the sound pressure level of people talking at a normal level, was adopted as the sound stimulus to measure sound fields at 5, 10, 15, and 20 mm from the entrance of the external auditory canal. The stimulus frequencies of 200, 500, 1000, 2000, 4000, 6000, and 8000 Hz were noted. The differences in sound field gains at depths of 5, 10, 15, and 20 mm were analyzed for the various mouth-opening levels. The results show that except for the 1.70-dB decrease at a depth of 5 mm with an 8000-Hz stimulus frequency, the differences at the other depths and stimulus frequencies were within 1 dB. Thus, the mouth-opening levels will influence the gain in ear canal. © 2016, German Acoustical Society (DEGA). All rights reserved.

关键词： Acoustic fields

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Comparison of the mechanical properties of the intraluminal thrombus in abdominal aortic aneurysms and thrombus mimics

Comparison of the mechanical properties of the intraluminal ...

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10th ASME Summer Bioengineering Conference, SBC2008

作者： Ashton, John H. Haskett, Darren G. Vande Geest, Jonathan P. Biomedical Engineering Interdisciplinary Program University of Arizona Tucson AZ United States Department of Agriculture and Bio Systems Engineering University of Arizona Tucson AZ United States Department of Aerospace and Mechanical Engineering University of Arizona Tucson AZ United States

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BPS2025 - Spatial compartmentalization of the expression of CaM genes supports versatile Ca 2+ -CaM signaling in cardiac myocytes

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Biophysical Journal 2025年第3期124卷 109a-110a页

作者： Vladimir Bogdanov Juan Ignacio Elio Mariangelo Anastasiia Pokrass Svetlana Tikunova Galina Sakuta Andrew M. Soltisz Benjamin Hernandez Orengo Casey Beard Jenna Thuma Thomas J. Hund Rengasayee Veeraraghavan Jonathan P. Davis Sandor Gyorke Department of Physiology and Cell Biology The Ohio State University Columbus OH USA The Ohio State University Columbus OH USA Department of Biomedical Engineering The Ohio State University Columbus OH USA Interdisciplinary Biophysics Graduate Program The Ohio State University Columbus OH USA Davis Heart and Lung Research Institute The Ohio State University Columbus OH USA

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Complete mitochondrial genome of Kishi velvet shrimp,Metapenaeopsis dalei(Rathbun, 1902) (Crustacea: Decapoda: Penaeidae) in the East China Sea

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Mitochondrial DNA Part B 2016年第1期1卷 120-121页

作者： Gyungryul Kim Tae-Ho Yoon Hye-Eun Kang Interdisciplinary Program of Biomedical Engineering Pukyong National University Department of Marine Biology Pukyong National University

We determined the complete mitochondrial genome ofMetapenaeopsis dalei(Rathbun, 1902), which is collected from East China Sea (124°E and 33.5°N). Total mitochondrial genome length ofM. daleiwas 15 939 bp, in which 13 proteins, two ribosomal RNAs, 22 transfer RNAs and a putative control region were encoded. The gene arrangement ofM. daleiwas well conserved with nine known Penaeidae mitochondrial genomes fromCOX1totRNATyr. The protein-coding genes started with ATN except forCOX1in which ACG is used. Four genes (COX2, COX3, ND3andND5) exhibited an incomplete stop codon. Nucleotide sequence identity ofM. daleimitochondrial genome to those of nine Penaeidae species ranged from 78% to 80%. Based on the COI region,M. daleiis most closely related toPenaeus notialis.

关键词： Barcode decapod crustacean mitochondrial genome Palaemon

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11th ASME Summer Bioengineering Conference, SBC2009

作者： Haskett, Darren Johnson, Greg Geest, Jonathan Vande Department of Agriculture and Bio Systems Engineering University of Arizona Tucson AZ United States Department of Aerospace and Mechanical Engineering University of Arizona Tucson AZ United States Biomedical Engineering Interdisciplinary Program University of Arizona Tucson AZ United States

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Accurate and rapid calculation of hydration free energy and its physical implication for biomolecular functions

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Biophysical Reviews 2020年第2期12卷 469-480页

作者： Kinoshita, Masahiro Hayashi, Tomohiko Institute of Advanced Energy Kyoto University Uji Kyoto 611-0011 Japan Interdisciplinary Program of Biomedical Engineering Assistive Technology and Art and Sports Sciences Faculty of Engineering Niigata University 8050 Ikarashi 2-no-cho Nishi-ku Niigata 950-2181 Japan

Here we review a new method for calculating a hydration free energy (HFE) of a solute and discuss its physical implication for biomolecular functions in aqueous environments. The solute hydration is decomposed into processes 1 and 2. A cavity matching the geometric characteristics of the solute at the atomic level is created in process 1. Solute-water van der Waals and electrostatic interaction potentials are incorporated in process 2. The angle-dependent integral equation theory combined with our morphometric approach is applied to process 1, and the three-dimensional reference interaction site model theory is employed for process 2. Molecular models are adopted for water. The new method is characterized by the following. Solutes with various sizes including proteins can be treated in the same manner. It is almost as accurate as the molecular dynamics simulation despite its far smaller computational burden. It enables us to handle a solute possessing a significantly large total charge without difficulty. The HFE can be decomposed into a variety of physically insightful, energetic, and entropic components. It is best suited to the elucidation of mechanisms of protein folding, pressure and cold denaturation of a protein, and different types of molecular recognition. © 2020, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature.

关键词： Biomolecule Hydration energy Hydration entropy Hydration free energy Integral equation theory Molecular recognition Morphometric approach Protein folding

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Behaviors of stem cells on carbon nanotube

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Biomaterials Research 2015年第1期19卷 1-6页

作者： Lee, Ju-Ro Ryu, Seungmi Kim, Soojin Kim, Byung-Soo School of Chemical and Biological Engineering Seoul National University Seoul 151-744 South Korea Interdisciplinary Program for Bioengineering Seoul National University Seoul 151-744 South Korea Department of Biomedical Engineering University of Alabama at Birmingham Birmingham AL United States

Regulating stem cell microenvironment is one of the essential elements in stem cell culture. Recently, carbon nanotube (CNT) has come into the spotlight as a biomaterial that retains unique properties. Based on its high chemical stability, elasticity, mechanical strength, and electrical conductivity, CNT shows great potential as an application for biomedical substrate. Also, properties of CNT could be further regulated by appropriate chemical modifications of CNT. Recent studies reported that modulating the cellular microenvironment through the use of CNT and chemically modified CNT as cell culture substrates can affect proliferation and differentiation of various types of stem cells. This review summarizes the unique biological effects of CNT on stem cells. © 2015 Lee et al.

关键词： Carbon nanotube Differentiation Stem cell Substrate

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