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Serum proteomic approach to identifying differentially expressed proteins in effusive feline infectious peritonitis

Scientific reports 2025年第1期15卷 18899页

作者： Wassamon Moyadee Sittiruk Roytrakul Janthima Jaresitthikunchai Narumon Phaonakrop Kiattawee Choowongkomon Sekkarin Ploypetch Natthasit Tansakul Amonpun Rattanasrisomporn Jatuporn Rattanasrisomporn Graduate Program in Animal Health and Biomedical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand. Department of Companion Animal Clinical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand. National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Pathum Thani Thailand. Department of Biochemistry Faculty of Science Kasetsart University Bangkok Thailand. Department of Clinical Sciences and Public Health Faculty of Veterinary Science Mahidol University Nakhon Pathom Thailand. Department of Pharmacology Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand. Interdisciplinary of Genetic Engineering and Bioinformatics Graduate School Kasetsart University Bangkok Thailand. Graduate Program in Animal Health and Biomedical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand. fvejpn@ku.ac.th. Department of Companion Animal Clinical Sciences Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand. fvejpn@ku.ac.th.

Feline infectious peritonitis (FIP) is a lethal, viral-induced immune-mediated disease that remains a challenge for diagnosis and treatment in cats. Proteomic profiling, which analyzes the protein content of biological samples, offers the potential to identify novel biomarkers that could improve the diagnosis and management of FIP. This study aims to assess the serum proteome and identify proteins that differentiate healthy cats from cats diagnosed with effusive FIP using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). A total of 30 cats diagnosed with effusive FIP and 27 clinically normal cats were enrolled. Twenty-three proteins were significantly (p < 0.01, ≥ fivefold change in abundance) differentially expressed between cats with effusive FIP and controls. Among these, the P2X purinoceptor, DNA topoisomerase, Notch receptor 2, and cadherin-17 were identified as key proteins of interest in cats with effusive FIP. Our findings suggest that these differentially expressed proteins could serve as potential diagnostic biomarkers and therapeutic targets for FIP. However, further studies are needed to validate these findings and explore their potential applications.

关键词： Biomarkers Cats Coronavirus Feline infectious peritonitis Proteomics Serum

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Synthesize palladium nanoparticles from the macroalgae Sargassum fusiforme: An eco-friendly tool in the fight against Plasmodium falciparum?

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The Science of the total environment 2023年第PT 3期857卷 159517页

作者： Chinnaperumal Kamaraj Chinnasamy Ragavendran R C Satish Kumar Sarvesh Sabarathinam Cittrarasu Vetrivel Mariyappan Vaithiyalingam Guilherme Malafaia Interdisciplinary Institute of Indian System of Medicine (IIISM) Drug Testing Laboratory Directorate of Research SRM Institute Science and Technology Kattankulathur 603 203 Tamil Nadu India. Electronic address: kamarajc@srmist.edu.in. Department of Cariology Saveetha Dental College and Hospitals Chennai Tamil Nadu India. Interdisciplinary Institute of Indian System of Medicine (IIISM) Drug Testing Laboratory Directorate of Research SRM Institute Science and Technology Kattankulathur 603 203 Tamil Nadu India. Carbon Capture Lab Department of Chemical Engineering SSN College of Engineering Kalavakkam Chennai 603110 Tamil Nadu India Department of Anatomy Saveetha Dental college and Hospital. Saveetha institute of medical and Technical science ChennaiTamil N- 600 007 India. Department of Chemistry SRM Institute of Science and Technology Kattankulathur Chengalpattu 603203 Tamilnadu India. Laboratory of Toxicology Applied to the Environment Goiano Federal Institute Urutaí GO Brazil Post-Graduation Program in Conservation of Cerrado Natural Resources Goiano Federal Institute Urutaí GO Brazil Post-Graduation Program in Ecology Conservation and Biodiversity Federal University of Uberlândia Uberlândia MG Brazil Post-Graduation Program in Biotechnology and Biodiversity Federal University of Goiás Goiânia GO Brazil. Electronic address: guilhermeifgoiano@***.

Although numerous drugs are practiced to control malaria and its vectors, more recently, eco-friendly control tools have been proposed to battle its etiologic agents. Thus, using green bionanotechnology approaches, we aimed to synthesize palladium nanoparticles (Pd NPs) from the macroalgae Sargassum fusiforme (Sf), its potential antiparasitic activity against P. falciparum, as well as its possible cytotoxicity, in HeLa cells. After the biosynthesis of the PdSf NPs, their characterization was carried out by UV-Vis, FESEM, and EDX analyses, and their hydrodynamic size, zeta potential, and surface area were determined. Furthermore, the functional groups of the PdSf NPs were analyzed by FT-IR and GC-MS. While PdSf NPs had an IC of 7.68 μg/mL (Chloroquine (CQ)-s) and 16.42 μg/mL, S. fusiforme extract had an IC of 14.38 μg/mL (CQ-s) and 35.27 μg/mL (CQ-r). With an IC value of 94.49 μg/mL, PdSf NPs exhibited the least toxic effect on the HeLa cells. The Lipinski rule of five and ADMET prediction were used to assess the in silico models of caffeine acid hexoside and quercetin 7-O-hexoside for the presence of drug-like properties. Pathogenic proteins, primarily responsible for motility, binding, and disease-causing, were the target of the structurally based docking studies between plant-derived compounds and pathogenic proteins. Thus, our study pioneered promising results that support the potential antiplasmodial activity of eco-friendly synthesized PdSf NPs using S. fusiforme extract against P. falciparum, opening perspectives for further exploration into the use of these NPs in malaria therapy.

关键词： Bionanotechnology Brown algae Molecular docking PdSf NPs Plasmodium falciparum Sargassum fusiforme

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Traditionally used medicinal plants mediate the biosynthesis of silver nanoparticles: methodological, larvicidal, and ecotoxicological approach

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The Science of the total environment 2023年 873卷 162402页

作者： Chinnaperumal Kamaraj Sugumar Vimal Chinnasamy Ragavendran Arumugam Priyadharsan K Marimuthu Guilherme Malafaia Interdisciplinary Institute of Indian System of Medicine (IIISM) Directorate of Research SRM Institute of Science and Technology (SRMIST) Kattankulathur 603203 Tamil Nadu India. Electronic address: kamarajc@srmist.edu.in. Department of Biochemistry Saveetha Medical College & Hospital Saveetha Institute of Medical and Technical Sciences (SIMATS) Thandalam Chennai 602105 Tamil Nadu India. Department of Conservative Dentistry and Endodontics Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Chennai 600 077 India. Department of Environmental Science Tezpur Univrsity Napaam Tezpur Assam 784028 India. Electronic address: kmmuthu@tezu.ernet.in. Laboratory of Toxicology Applied to the Environment Goiano Federal Institute Urutaí GO Brazil Post-Graduation Program in Conservation of Cerrado Natural Resources Goiano Federal Institute Urutaí GO Brazil Post-Graduation Program in Ecology Conservation and Biodiversity Federal University of Uberlândia Uberlândia MG Brazil Post-Graduation Program in Biotechnology and Biodiversity Federal University of Goiás Goiânia GO Brazil. Electronic address: guilhermeifgoiano@***.

It has been shown that vegetal species constitute an alternative natural source for the biosynthesis of new nanomaterials. Thus, aiming to expand knowledge about the potential use of plants in the fabrication of metallic nanomaterials, we aimed to synthesize silver nanoparticles (AgNPs) from phyto-formulation (PF) of ten commonly used medicinal plants. Our results demonstrate the formation of spherical, stable, polycrystalline AgNPs with a diameter of 8.42 nm to 18.40 nm, whose biosynthesis confirmation was performed via UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), field emission scanning electron microscopy (FE-SEM)-energy dispersive X-ray spectroscopy (EDS) mapping, high-resolution transmission electron microscopy (HR-TEM), dynamic light scattering (DLS), and zeta potential studies. Furthermore, we demonstrated that the biosynthesized AgNPs showed larvicidal activity against Aedes aegypti and Anopheles stephensi larvae, with the histopathology findings from the fourth instar larval stage validating such larvicidal toxicity. The histological examinations showed severe degradation of the larvae's hindgut, epithelial cells, midgut, and cortical area. However, the PF extract and the biosynthesized AgNPs showed high ecotoxicity in Danio rerio larvae exposed to different concentrations. The treatments induced changes in hatchability percentage, animal growth, and heartbeat. Therefore, despite supporting the potential of PF (from ten plant species) as a raw material source for AgNPs biosynthesis, our study also sheds light on its ecotoxicological potential, suggesting that more comprehensive assessments of the ecotoxicity of biosynthesized would be performed before its application in different sectors.

关键词： Biofabrication Ecotoxicity Nanomaterials Phyto-formulation Plant extract

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Author Correction: O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine

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Nature communications 2022年第1期13卷 6253页

作者： Kibum Kim Hee Chan Yoo Byung Gyu Kim Sulhee Kim Yulseung Sung Ina Yoon Ya Chun Yu Seung Joon Park Jong Hyun Kim Kyungjae Myung Kwang Yeon Hwang Sunghoon Kim Jung Min Han Interdisciplinary Program of Integrated OMICS for Biomedical Science Graduate School Yonsei University Seoul 03722 South Korea. Yonsei Institute of Pharmaceutical Sciences College of Pharmacy Yonsei University Incheon 21983 South Korea. Center for Genomic Integrity Institute for Basic Science Ulsan 44919 South Korea. Department of Biotechnology College of Life Sciences and Biotechnology Korea University Seoul 02841 South Korea. Institute for Artificial Intelligence and Biomedical Research Medicinal Bioconvergence Research Center Yonsei University Incheon 21983 South Korea. College of Medicine Gangnam Severance Hospital Yonsei University Seoul 06273 South Korea. Department of Biochemistry School of Medicine Catholic University of Daegu Daegu 42472 South Korea. Department of Biomedical Engineering Ulsan National Institute of Science and Technology Ulsan 44919 South Korea. Interdisciplinary Program of Integrated OMICS for Biomedical Science Graduate School Yonsei University Seoul 03722 South Korea. jhan74@yonsei.ac.kr. Yonsei Institute of Pharmaceutical Sciences College of Pharmacy Yonsei University Incheon 21983 South Korea. jhan74@yonsei.ac.kr. POSTECH Biotech Center Pohang University of Science and Technology Pohang 37673 South Korea. jhan74@yonsei.ac.kr.

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Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates

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Cell 2025年

作者： Yan, Xiao Kuster, David Mohanty, Priyesh Nijssen, Jik Pombo-García, Karina Garcia Morato, Jorge Rizuan, Azamat Franzmann, Titus M. Sergeeva, Aleksandra Ly, Anh M. Liu, Feilin Passos, Patricia M. George, Leah Wang, Szu-Huan Shenoy, Jayakrishna Danielson, Helen L. Ozguney, Busra Honigmann, Alf Ayala, Yuna M. Fawzi, Nicolas L. Dickson, Dennis W. Rossoll, Wilfried Mittal, Jeetain Alberti, Simon Hyman, Anthony A. Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) Saxony Dresden 01307 Germany Artie McFerrin Department of Chemical Engineering Texas A&M University College Station 77843 TX United States Department of Neuroscience Mayo Clinic Jacksonville 32224 FL United States Biotechnology Center (BIOTEC) Center for Molecular and Cellular Bioengineering Technische Universität Dresden Saxony Dresden 01307 Germany Edward Doisy Department of Biochemistry and Molecular Biology Saint Louis University St. Louis 63104 MO United States Department of Molecular Biology Cell Biology & Biochemistry Brown University Providence 02912 RI United States Center for Biomedical Engineering Brown University Providence 02912 RI United States Department of Chemistry Texas A&M University College Station 77843 TX United States Interdisciplinary Graduate Program in Genetics and Genomics Texas A&M University College Station 77843 TX United States

Cytosolic aggregation of the nuclear protein TAR DNA-binding protein 43 (TDP-43) is associated with many neurodegenerative diseases, but the triggers for TDP-43 aggregation are still debated. Here, we demonstrate that TDP-43 aggregation requires a double event. One is up-concentration in stress granules beyond a threshold, and the other is oxidative stress. These two events collectively induce intra-condensate demixing, giving rise to a dynamic TDP-43-enriched phase within stress granules, which subsequently transition into pathological aggregates. Intra-condensate demixing of TDP-43 is observed in iPS-motor neurons, a disease mouse model, and patient samples. Mechanistically, intra-condensate demixing is triggered by local unfolding of the RRM1 domain for intermolecular disulfide bond formation and by increased hydrophobic patch interactions in the C-terminal domain. By engineering TDP-43 variants resistant to intra-condensate demixing, we successfully eliminate pathological TDP-43 aggregates in cells. We suggest that up-concentration inside condensates followed by intra-condensate demixing could be a general pathway for protein aggregation. © 2025 The Author(s)

关键词： ALS biomolecular condensate FTD intra-condensate demixing neurodegenerative diseases oxidative stress phase separation protein aggregation stress granules TDP-43

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Immune enhancement effect of an herb complex extract through the activation of natural killer cells and the regulation of cytokine levels in a cyclophosphamide-induced immunosuppression rat model

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Asian Pacific Journal of Tropical Medicine 2018年第12期11卷 653-658页

作者： Sung Min Woo Woo Rin Choi Dooly Jang Chun Sik Yi Hae Lim Kim Kyung Hyeon Kim Jong Tae Kim Won Hee Choi Seung Hee Jang Min Jeung Kim Ji Hyang Wee Yeon Ki Kim Bao Le Interdisciplinary Program of Biomodulation Myongji UniversityYonginGyeonggi 17058Republic of Korea Center for Nutraceutical and Pharmaceutical Materials Myongji UniversityYonginGyeonggi 17058Republic of Korea Teazen Co. B-606 Acrotower230 Sinim-daeroDongan-guAnyang-siGyeonggi-do 14067Republic of Korea Department of Food Science&Biotechnology Shin Ansan UniversityAnsanGyeonggi 15435Republic of Korea' Division of Bioscience and Bioinformatics Myongji UniversityYonginGyeonggi 17058Republic of Korea Department of Biotechnology Chonnam National UniversityYeosuChonnam 59626Republic of Korea

Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro and in vivo analysis. Methods: The activity of natural killer(NK) cells was measured in splenocytes and YAC-1 cells. Mice were immunosuppressed using cyclophosphamide(5 mg/kg body weight). Three different doses of HCE(200, 400, and 800 mg/kg body weight) and red ginseng extract(800 mg/kg body weight) which was used as standard immunomodulatory herb were administered orally for 4 weeks. The body weight, dietary, water intake, organs(liver, thymus, and spleen) weight, completed blood count, and cytokines(tumor necrosis factor alpha, interferon gamma, and interleukin-2) production was measured. Results: At the maximum concentration of HCE, the activity of NK cells was increased by 48.5%. HCE increased liver, spleen, and thymus weights without altering numbers of white blood cells, lymphocytes, and neutrophils in a cyclophosphamide-induced immunosuppression rat model. However, HCE recovered the inhibited cytokine expression; HCE(800 mg/kg) increased cytokines levels. The results indicate the immune enhancement potential of this HCE. Conclusion: The HCE enhances immunity by increasing NK cell activity, regulating cytokine levels, and maintaining spleen weight. Therefore, it may be used as a potential immunity enhancer.

关键词： Immune system enhancement Cyclophosphamide Cornus officinalis Sieb Et Zucc Eriobotrya japonica Lindley Olive leaves

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Precision cancer medicine in ovarian cancer

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Cancer Letters 2024年 581卷 216546-216546页

作者： Kim, Se Ik Jo, HyunA. Kim, Heeyeon Lee, Juwon Cho, Untack Kim, Soochi Kim, Boyun Seol, Aeran Lee, Maria Lee, Cheol Ahn, Taejin Song, Yong Sang Department of Obstetrics and Gynecology Seoul National University College of Medicine Seoul 03080 Republic of Korea Cancer Research Institute Seoul National University College of Medicine Seoul 03080 Republic of Korea WCU Biomodulation Department of Agricultural Biotechnology Seoul National University Seoul 08826 Republic of Korea Department of Neurology and Neurological Sciences Stanford University School of Medicine Stanford CA 94305 USA Paul F. Glenn Laboratories for the Biology of Aging Stanford University School of Medicine Stanford CA 94305 USA Department of SmartBio Kyungsung University College of Life and Health Science Busan 48434 Republic of Korea Department of Obstetrics and Gynecology Korea University Anam Hospital College of Medicine Korea University Seoul 02841 Republic of Korea Department of Pathology Seoul National University College of Medicine Seoul 03080 Republic of Korea Department of Life Science Handong Global University Pohang-si Gyeongbuk 37554 Republic of Korea Interdisciplinary Program in Cancer Biology Seoul National University College of Medicine Seoul 03080 Republic of Korea

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Plant immune responses - from guard cells and local responses to systemic defense against bacterial pathogens

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Plant Signaling and Behavior 2019年第5期14卷 Article: e1588667页

作者： David, Lisa Harmon, Alice C. Chen, Sixue Department of Biology University of Florida Gainesville FL United States University of Florida Genetics Institute (UFGI) Gainesville FL United States Plant Molecular and Cellular Biology Program University of Florida Gainesville FL United States Proteomics and Mass Spectrometry Interdisciplinary Center for Biotechnology Research (ICBR) University of Florida Gainesville FL United States

When plants are infected by pathogens two distinct responses can occur, the early being a local response in the infected area, and later a systemic response in non-infected tissues. Closure of stomata has recently been found to be a local response to bacterial pathogens. Stomata closure is linked to both salicylic acid (SA), an essential hormone in local responses and systemic acquired resistance (SAR), and absisic acid (ABA) a key regulator of drought and other abiotic stresses. SAR reduces the effects of later infections. In this review we discuss recent research elucidating the role of guard cells in local and systemic immune responses, guard cell interactions with abiotic and hormone signals, as well as putative functions and interactions between long-distance SAR signals. © 2019, © 2019 Taylor & Francis Group, LLC.

关键词： guard cells local immune responses mobile signals Plant immunity systemic defense

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Author Correction: Mitonuclear incompatibility as a hidden driver behind the genome ancestry of African admixed cattle

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BMC biology 2022年第1期20卷 62页

作者： Taehyung Kwon Kwondo Kim Kelsey Caetano-Anolles Samsun Sung Seoae Cho Choongwon Jeong Olivier Hanotte Heebal Kim Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National University Seoul South Korea. eGnome Inc. Seoul South Korea. Callout Biotech Albuquerque New Mexico United States. School of Biological Sciences Seoul National University Seoul South Korea. School of Life Sciences University of Nottingham Nottingham UK. olivier.hanotte@nottingham.ac.uk. LiveGene International Livestock Research Institute (ILRI) Addis Ababa Ethiopia. olivier.hanotte@nottingham.ac.uk. The Centre for Tropical Livestock Genetics and Health (CTLGH) The Roslin Institute The University of Edinburgh Edinburgh UK. olivier.hanotte@nottingham.ac.uk. Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National University Seoul South Korea. heebal@snu.ac.kr. eGnome Inc. Seoul South Korea. heebal@snu.ac.kr. Interdisciplinary Program in Bioinformatics Seoul National University Seoul South Korea. heebal@snu.ac.kr.

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A Machine Learning-Based Approach for Classification of Alzheimer’s Disease and its Risk Prediction

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Alzheimer's & Dementia 2022年第S11期18卷

作者： Payam Hosseinzadeh Kasani Jung-Kyeom Kim Peyman Hosseinzadeh Kassani Yeshin Kim Jeong-Ah Kim Chihyun Park Cheol-Heui Yun Seong Hye Choi Sang-Ah Lee Seo-Young Lee Jae-Won Jang Department of Neurology Kangwon National University Hospital Chuncheon Korea Republic of (South) Interdisciplinary Graduate Program in Medical Bigdata Convergence Kangwon National University Chuncheon Korea Republic of (South) Department of Neurology and Neurological Sciences Stanford University Stanford CA USA College of Medicine Kangwon National University Chuncheon Korea Republic of (South) Department of Ophthalmology Kangwon National University Hospital Chuncheon Korea Republic of (South) Department of Computer Science and Engineering Kangwon National University Chuncheon Korea Republic of (South) Department of Agricultural Biotechnology Seoul National University Seoul Korea Republic of (South) Research Institute of Agriculture and Life Sciences Seoul National University Seoul Korea Republic of (South) Department of Neurology Inha University School of Medicine Incheon Korea Republic of (South) Department of Preventive Medicine Kangwon National University School of Medicine Chuncheon Korea Republic of (South)

Background Dementia, a clinical syndrome with progressive neurodegenerative disease, is one of the inevitable aging consequences. Alzheimer’s disease (AD) is the most common cause of dementia. Accurate detection and classification of Alzheimer’s disease alongside its conversion from Mild Cognitive Impairment (MCI) to AD at an early stage is of significant clinical importance. However, a reliable diagnosis with a multi-label classification approach remains a challenging task. This study aimed to measure the effectiveness of machine learning algorithm integrated with feature importance techniques to constitutes a multi-label classification of AD prediction for clinical practice. Method The machine learning algorithms are validated using a nationwide cohort dataset, the Korean Brain Aging Study for the early diagnosis and prediction of AD (KBASE) to identify the most important risk factors, where a database is classified into three classes namely, normal cognition (NC, n = 91), mild cognitive impairment (MCI, n = 61), and Alzheimer’s disease (AD, n = 49). Applying eight state-of-the-art supervised machine learning algorithms namely Support Vector Machine, Naive Bayes, XGBoost, Decision Tree, Logistic Regression, Random Forest, Bagging, and AdaBoost. A stratified 10-fold cross-validation was used for every train/test split and several performance measures, including accuracy, precision, recall and f1-score were used to compare the classifier performances. Result Based on the performance evaluation, the XGBoost classifier significantly outperformed all other models and achieved 82.55% accuracy followed by the Bagging classifier (81%). According to the XGBoost model, calculating risk factors to predict performance, the Consortium to Establish a Registry for AD (CERAD) subsets is among the highest ranked feature. It was intriguing that the parameters including job, job levels and retirement achieved a minimal, if any, impact in the final performance. Conclusion Collectivel

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