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Efficient (S)-acetoin production in Saccharomyces cerevisiae by modulating α-acetolactate decarboxylase stereospecificity

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Bioresource technology 2025年 434卷 132767页

作者： Seungwoo Cha Byeongseon Jang Daeyeol Lee Injae Cho Wooyoung Park Youngmin Lee Hyesoo Shin Minyoung Kim Changmin Sung Ji-Sook Hahn Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: mashmalo@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: qudtjs5095@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: greenberry05@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: ept9796@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: wypkr@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: kevinlee9@snu.ac.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: shs6482@snu.ac.kr. Doping Control Center Korean Institute of Science and Technology 5 Hwarang-ro 14-gil Seongbuk-gu Seoul 02792 Republic of Korea Interdisciplinary Program for Biochemical Engineering and Biotechnology Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea. Electronic address: kmy5@kist.re.kr. Doping Control Center Korean Institute of Science and Technology 5 Hwarang-ro 14-gil Seongbuk-gu Seoul 02792 Republic of Korea. Electronic address: scm@kist.re.kr. Department of Chemical and Biological Engineering Institute of Chemical Processes Seoul National Univ

Acetoin, a four-carbon α-hydroxyketone with a chiral center, exists as two stereoisomers-(R)- and (S)-acetoin-both of which hold potential pharmaceutical applications. While microbial production of (R)-acetoin has been well studied, fermentative production of (S)-acetoin remains limited due to its reliance on non-enzymatic spontaneous decarboxylation of unstable intermediates. As a result, costly precursors like diacetyl or 2,3-butanediol are often used, posing challenges for economic scale-up. In this study, we engineered Saccharomyces cerevisiae to produce stereospecific (S)-acetoin directly from glucose, a low-cost and abundant carbon source. The native racemic acetoin pathway was eliminated by disrupting pyruvate decarboxylase (PDC) activity, and the stereospecificity of Bacillus subtilis α-acetolactate decarboxylase (ALDC), which naturally favors (R)-acetoin, was engineered to preferentially produce (S)-acetoin. Oxygen uptake was genetically enhanced to support efficient conversion of intermediates and suppress byproducts. This work established a novel, enzyme-driven pathway for (S)-acetoin biosynthesis with over 90% enantiomeric purity, without the addition of chemical compounds.

关键词： (S)-Acetoin Enzyme engineering Metabolic engineering Oxygen transfer Pyruvate decarboxylase Saccharomyces cerevisiae Stereoselectivity

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Emerging contaminants:A One Health perspective

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The Innovation 2024年第4期5卷 140-170,139页

作者： Fang Wang Leilei Xiang Kelvin Sze-Yin Leung Martin Elsner Ying Zhang Yuming Guo Bo Pan Hongwen Sun Taicheng An Guangguo Ying Bryan WBrooks Deyi Hou Damian EHelbling Jianqiang Sun Hao Qiu Timothy MVogel Wei Zhang Yanzheng Gao Myrna JSimpson Yi Luo Scott XChang Guanyong Su Bryan MWong Tzung-May Fu Dong Zhu Karl JJobst Chengjun Ge Frederic Coulon Jean Damascene Harindintwali Xiankui Zeng Haijun Wang Yuhao Fu Zhong Wei Rainer Lohmann Changer Chen Yang Song Concepcion Sanchez-Cid Yu Wang Ali El-Naggar Yiming Yao Yanran Huang Japhet Cheuk-Fung Law Chenggang Gu Huizhong Shen Yanpeng Gao Chao Qin Hao Li Tong Zhang Natàlia Corcoll Min Liu Daniel SAlessi Hui Li Kristian KBrandt Yolanda Pico Cheng Gu Jianhua Guo Jianqiang Su Philippe Corvini Mao Ye Teresa Rocha-Santos Huan He Yi Yang Meiping Tong Weina Zhang Fidèle Suanon Ferdi Brahushi Zhenyu Wang Syed AHashsham Marko Virta Qingbin Yuan Gaofei Jiang Louis A.Tremblay Qingwei Bu Jichun Wu Willie Peijnenburg Edward Topp Xinde Cao Xin Jiang Minghui Zheng Taolin Zhang Yongming Luo Lizhong Zhu Xiangdong Li DamiàBarceló Jianmin Chen Baoshan Xing Wulf Amelung Zongwei Cai Ravi Naidu Qirong Shen Janusz Pawliszyn Yong-guan Zhu Andreas Schaeffer Matthias C.Rillig Fengchang Wu Gang Yu James M.Tiedje State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Hong Kong Baptist UniversityHong KongChina HKBU Institute of Research and Continuing Education Shenzhen Virtual University ParkShenzhenChina Technical University of Munich TUM School of Natural SciencesInstitute of Hydrochemistry85748 GarchingGermany School of Resources&Environment Northeast Agricultural UniversityHarbin 150030China Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourneVICAustralia Faculty of Environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500China Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China Ministry of Education Key Laboratory of Environmental Theoretical Chemistry South China Normal UniversityGuangzhouGuangdong 510006China Department of Environmental Science Baylor UniversityWacoTXUSA Center for Reservoir and Aquatic Systems Research(CRASR) Baylor UniversityWacoTXUSA School of Environment Tsinghua UniversityBeijing 100084China School of Civil and Environmental Engineering Cornell UniversityIthacaNY 14853USA Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province Zhejiang University of TechnologyHangzhou 310014China School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Laboratoire d’Ecologie Microbienne Universite Claude Bernard Lyon 1UMR CNRS 5557UMR INRAE 1418VetAgro Sup69622 VilleurbanneFrance Department of Pla

Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary *** global efforts to mitigate legacy pollutants,the continuous introduction of new substances remains a major threat to both people and the *** response,global initiatives are focusing on risk assessment and regulation of emerging contaminants,as demonstrated by the ongoing efforts to establish the UN’s Intergovernmental Science-Policy Panel on Chemicals,Waste,and Pollution *** review identifies the sources and impacts of emerging contaminants on planetary health,emphasizing the importance of adopting a One Health *** for monitoring and addressing these pollutants are discussed,underscoring the need for robust and socially equitable environmental policies at both regional and international *** actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

关键词： pollution planet contaminants

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Quality and readability of online information resources on insomnia

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Frontiers of Medicine 2017年第3期11卷 423-431页

作者： Yan Ma Albert C. Yang Ying Duan Ming Dong Albert S. Yeung Division of Interdisciplinary Medicine and Biotechnology Department of Medicine Beth Israel Deaconess Medical Center Harvard Medical School Boston MA 02215 USA Sleep Medicine Center Airforee General Hospital Beijing 100142 China IBM Software Development Lab Littleton MA 01460 USA Depression Clinical and Research Program Massachusetts General Hospital Harvard Medical School Boston MA 02114 USA

The internet is a major source for health information. An increasing number of people, including patients with insomnia, search for remedies online; however, little is known about the quality of such information. This study aimed to evaluate the quality and readability of insomnia-related online information. Google was used as the search engine, and the top websites on insomnia that met the inclusion criteria were evaluated for quality and readability. The analyzed websites belonged to nonprofit, commercial, or academic organizations and institutions such as hospitals and universities. Insomnia-related websites typically included definitions （85%）, causes and risk factors （100%）, symptoms （95%）, and treatment options （90%）. Cognitive behavioral therapy for insomnia （CBT-I） was the most commonly recommended approach for insomnia treatment, and sleep drugs are frequently mentioned. The overall quality of the websites on insomnia is moderate, but all the content exceeded the recommended reading ease levels. Concerns that must be addressed to increase the quality and trustworthiness of online health information include sharing metadata, such as authorship, time of creation and last update, and conflicts of interest; providing evidence for reliability; and increasing the readability for a layman audience.

关键词： insomnia intemet readability information quality health literacy cognitive behavioral therapy treatment

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Correction to: Dietary intervention using (1,3)/(1,6)-β-glucan, a fungus-derived soluble prebiotic ameliorates high-fat diet-induced metabolic distress and alters beneficially the gut microbiota in mice model

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European journal of nutrition 2021年第4期60卷 2273页

作者： Karthika Muthuramalingam Vineet Singh Changmin Choi Seung In Choi Young Mee Kim Tatsuya Unno Moonjae Cho Department of Biochemistry School of Medicine Jeju National University Jeju 63243 Republic of Korea. Faculty of Biotechnology School of Life Sciences SARI Jeju National University Jeju 63243 Republic of Korea. Department of Pharmaceutical Research Institute Quegen Biotech Co. Ltd Seoul 429931 Republic of Korea. Faculty of Biotechnology School of Life Sciences SARI Jeju National University Jeju 63243 Republic of Korea. tatsu@jejunu.ac.kr. Subtropical/Tropical Organism Gene Bank Jeju National University Jeju 63243 Republic of Korea. tatsu@jejunu.ac.kr. Department of Biochemistry School of Medicine Jeju National University Jeju 63243 Republic of Korea. moonjcho@jejunu.ac.kr. Institute of Medical Science Jeju National University Jeju 63241 Republic of Korea. moonjcho@jejunu.ac.kr. Interdisciplinary Graduate Program in Advanced Convergence Technology & Science Jeju National University Jeju 63241 Republic of Korea. moonjcho@jejunu.ac.kr.

A correction to this paper has been published: https://***/10.1007/s00394-021-02537-9

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Corrigendum to "Dual regulatory effects of neferine on amyloid-β and tau aggregation studied by in silico, in vitro, and lab-on-a-chip technology" [Biomed. Pharmacother. 172 (2024) 1-14/116226]

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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024年 181卷 117686页

作者： Yunkwon Nam Ritu Prajapati Sujin Kim Soo Jung Shin Da Yeon Cheong Yong Ho Park Hyun Ha Park Danyou Lim Yoojeong Yoon Gyudo Lee Hyun Ah Jung Insu Park Dong-Hyun Kim Jae Sue Choi Minho Moon Department of Biochemistry College of Medicine Konyang University 158 Gwanjeodong-ro Seo-gu Daejeon 35365 Republic of Korea. Department of Food and Life Science Pukyong National University Busan 48513 Republic of Korea. Department of Biochemistry College of Medicine Konyang University 158 Gwanjeodong-ro Seo-gu Daejeon 35365 Republic of Korea Research Institute for Dementia Science Konyang University 158 Gwanjeodong-ro Seo-gu Daejeon 35365 Republic of Korea. Department of Biotechnology and Bioinformatics Korea University Sejong 30019 Republic of Korea Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology Korea University Sejong 30019 Republic of Korea. Department of Biomedical Engineering Konyang University Daejeon 35365 Republic of Korea. Department of Food Science and Human Nutrition Chonbuk National University Jeonju 54896 Republic of Korea. Department of Biomedical Engineering Konyang University Daejeon 35365 Republic of Korea. Electronic address: insupark@konyang.ac.kr. Departments of Pharmacology and Advanced Translational Medicine School of Medicine Konkuk University Seoul 05029 Republic of Korea. Electronic address: mose79@kku.ac.kr. Department of Food and Life Science Pukyong National University Busan 48513 Republic of Korea. Electronic address: choijs@pknu.ac.kr. Research Institute for Dementia Science Konyang University 158 Gwanjeodong-ro Seo-gu Daejeon 35365 Republic of Korea. Electronic address: hominmoon@konyang.ac.kr.

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Frontispiz: 3‐O‐Sulfation of Heparan Sulfate Enhances Tau Interaction and Cellular Uptake

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Angewandte Chemie 2020年第5期132卷

作者： Jing Zhao Yanan Zhu Xuehong Song Yuanyuan Xiao Guowei Su Xinyue Liu Zhangjie Wang Yongmei Xu Jian Liu David Eliezer Trudy F. Ramlall Guy Lippens James Gibson Fuming Zhang Robert J. Linhardt Lianchun Wang Chunyu Wang Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute Troy NY USA Department of Molecular Pharmacology and Physiology University of South Florida Tampa USA Division of Chemical Biology and Medicinal Chemistry Eshelman School of Pharmacy University of North Carolina Chapel Hill USA Department of Biochemistry Program in Structural Biology Weill Cornell Medical College New York NY USA Toulouse Biotechnology Institute CNRS INRA INSA University of Toulouse 31077 Toulouse France Department of Chemistry and Chemical Biology Rensselaer Polytechnic Institute Troy NY USA Byrd Alzheimer's Research Institute Morsani College of Medicine University of South Florida Tampa USA Department of Biological Sciences Rensselaer Polytechnic Institute Troy NY USA

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Frontispiece: 3-O-Sulfation of Heparan Sulfate Enhances Tau Interaction and Cellular Uptake

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Angewandte Chemie International Edition 2020年第5期59卷

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Molecular characterization and expression analysis of Cyclin B and Cell division cycle 2 in gonads of diploid and triploid bighead catfish, Clarias macrocephalus Günther, 1864

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Agriculture and Natural Resources 2017年第2期51卷 117-130页

作者： Wachirachaikarn, Anyalak Rungsin, Wikrom Srisapoome, Prapansak Klinbunga, Sirawut Na-Nakorn, Uthairat Interdisciplinary Program in Genetic Engineering Graduate School Kasetsart University Bangkok 10900 Thailand Department of Zoology Faculty of Science Kasetsart University Bangkok 10900 Thailand Department of Aquaculture Faculty of Fisheries Kasetsart University Bangkok 10900 Thailand Aquatic Molecular Genetics and Biotechnology Laboratory National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Khlong Nueng Khlong Luang Pathum Thani 12120 Thailand Center of Excellence for Marine Biotechnology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand

This study investigated the differential expression of genes associated with reproduction in sterile triploid and normal diploid bighead catfish (Clarias macrocephalus Günther, 1864). The triploid fish were produced using cold shock and were reared in the same conditions as the diploid counterpart. The histomicrographs showed completely retarded triploid gonads across the samples aged 2–12 mth, whereas the gonads of the diploids were in developing stages during 2–4 mth, reached the early maturing stage at 6 mth, matured at 8 mth and showed signs of atresia at 10–12 mth. In parallel, the full-length cDNAs of cyclin B1 (CmCcnb1;1539 bp in length with an open reading frame (ORF) of 1194 bp corresponding to 397 amino acids) and cell division cycle 2 (CmCdc2;1355 bp, an ORF of 909 bp, 302 amino acids) of bighead catfish (C. macrocephalus Günther, 1864) were isolated. Phylogenetic analysis revealed that the newly characterized CmCcnb1 should be regarded as a member of cyclin B1 rather than cyclin B2. The expression level of CmCcnb1 mRNA was limited in different stages of the ovaries and testes of triploids. In diploid ovaries, its expression was significantly higher than that in triploid ovaries in fish aged 2 mth (513.43 ± 82.22 fold) and in fish aged 8 mth (2430.87 ± 900.06 fold). The CmCcnb1 level in the testes of diploids was significantly greater than that in triploids in fish aged 2 mth (928.85 ± 208.72 fold). Similarly, expression of CmCdc2 mRNA was also reduced in triploids. Its expression was significantly lower than that in diploid females aged 2 mth (7.66 ± 3.42 fold), 4 mth (59.42 ± 10.50 fold) and 8 mth (42.74 ± 8.36 fold). In males, significantly greater expression of CmCdc2 was observed at age 6 mth (58.61 ± 34.64 fold) and 8 mth (72.70 ± 4.36 fold) diploids compared to triploids. The results illustrated that CmCcnb1 and CmCdc2 are functionally involved in oogenesis and spermatogenesis and reduced expression levels of these transcripts affected the reproduc

关键词： Cdc2 Clarias macrocephalus Cyclin B Diploids Expression Triploids

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Author Correction: Towards symbiotic approaches between universities, sustainable development, and cities

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Scientific reports 2023年第1期13卷 552页

作者： Walter Leal Filho Liliana Caughman Maria Alzira Pimenta Dinis Fernanda Frankenberger Anabela Marisa Azul Amanda Lange Salvia Department of Natural Sciences Manchester Metropolitan University Chester Street Manchester M115GD UK. Research and Transfer Centre "Sustainable Development and Climate Change Management" Hamburg University of Applied Sciences Ulmenliet 20 21033 Hamburg Germany. Postdoctoral Research Scholar Earth Systems Science for the Anthropocene Arizona State University Tempe USA. UFP Energy Environment and Health Research Unit (FP-ENAS) University Fernando Pessoa (UFP) Praça 9 de Abril 349 4249-004 Porto Portugal. Business School PUCPR Pontifical Catholic University of Paraná Rua Imaculada Conceição Curitiba 1155 Brazil. Brazil Business School Universidade Positivo Rua Pedro Viriato Parigot de Souza Curitiba 5300 Brazil. CNC-Center for Neuroscience and Cell Biology University of Coimbra 3004-504 Coimbra Portugal. CIBB-Center for Innovative Biomedicine and Biotechnology University of Coimbra Coimbra Portugal. IIIUC-Institute for Interdisciplinary Research University of Coimbra 3030-789 Coimbra Portugal. Graduate Program in Civil and Environmental Engineering University of Passo Fundo Campus I - BR285 São José Passo Fundo RS 99052-900 Brazil. amandasalvia@***. European School of Sustainability Science and Research Hamburg University of Applied Sciences Ulmenliet 20 21033 Hamburg Germany. amandasalvia@***.

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Topological data analysis can extract subgroups with high incidence rates of Type 2 diabetes

Topological data analysis can extract subgroups with high in...

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IEEE International Conference on Bioinformatics and Biomedicine

作者： Hyung Sun Kim Changwoo Yi Yongkang Kim Uhnmee Park Woong Kook Hyuk Kim Taesung Park Interdisciplinary Program in bioinformatics Seoul National University The Research Institute of Basic Sciences College of Natural Sciences Seoul National University Department of Statistics Seoul National University Department of Bioscience and biotechnology University of Suwon Department of Mathematical Sciences and Institute for Mathematical Data Analytics Research Center Seoul National University Department of Mathematical Sciences Seoul National University

ISBN: (纸本)9781538654897;9781538654880

Type 2 diabetes (T2D) is now a rapidly increasing, worldwide scourge, and the identification of genetic contributors is vital. However, current analyses of multiple, disease-contributing factors, and their combined interactions, remains quite difficult, using traditional approaches. Topological data analysis (TDA) shows what shape a data set can have, facilitating clustering analysis, by determining which components are close to each other. Thus, TDA can generate a network, using single-nucleotide polymorphism (SNP) data, revealing the genetic relatedness of specific individuals, and can derive multiple ordered subgroups, from one with a low patient concentration, to one with a high patient concentration. Since it is widely accepted that T2D pathogenesis is affected by multiple genetic factors, we performed TDA on T2D data from the Korea Association REsource (KARE) project, a population-based, genome-wide association study of the Korean adult population. Since KARE data contains follow-up information about the incidence of T2D, we compared the T2D status of each individual, at baseline, with that of 10 years later. For the TDA network-driven subgroups, ordered by prevalence, we compared the T2D incidence rate, after 10 years, for individuals initially without T2D. As a result, we found that the TDA network-driven, ordered subgroups had significantly increased incidence rates, linearly correlated with prevalence (p-value = 0.006914). Our results demonstrate the usefulness of TDA in both identifying genetic contributors (e.g., SNPs), and their interrelationships, in the pathology of complex diseases.

关键词： Data analysis Genetics Bioinformatics Diabetes Diseases Conferences Data mining

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