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IEEE International Symposium on Consumer Electronics

作者： Lee, D.-K. Park, R.-H. Chang, S. Department of Electronic Engineering School of Engineering Sogang University Seoul 121-742 Korea Republic of Interdisciplinary Program of Integrated Biotechnology Sogang University 1 Sinsu-dong Mapo-gu Seoul 121-742 Korea Republic of Samsung Electronics Co. Ltd. Suwon Gyeonggi-do 443-742 Korea Republic of

ISBN: (纸本)9781612848433

For producing a single high dynamic range image (HDRI), multiple low dynamic range images (LDRIs) with different exposures are used. In high dynamic range imaging, a moving object in a set of LDRIs causes the ghost effect in a final HDRI. In this paper, we propose a ghost removal method based on histogram information. Object motion is detected by comparing ranks of intensity between LDRIs, and then the generated motion map is combined to construct the weight map in exposure fusion. Experimental results using various sets of LDRIs show that the proposed method effectively removes ghost effect and enhances contrast in a final HDRI. In terms of the computation time, the proposed method gives a better performance than existing methods. © 2011 IEEE.

关键词： Image enhancement

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Protein-based multi-bit biomemory device consisting of various metalloproteins on self-assembled 11-MUA layer

Protein-based multi-bit biomemory device consisting of vario...

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作者： Lee, Taek Min, Junhong Lee, Jin-Ho Choi, Jeong-Woo Department of Chemical and Biomolecular Engineering Sogang University 1 Shinsu-dong Mapo-gu Seoul 121-742 Korea Republic of Interdisciplinary Program of Integrated Biotechnology Sogang University 1 Shinsu-dong Mapo-gu Seoul 121-742 Korea Republic of College of Bionanotechnology Kyungwon University Bokjung-dong Sujung-gu Seongnam 461-701 Korea Republic of

A multi-bit biomemory device was devised by introducing 4 different metalloproteins (azurin, cytochrome c, ferredoxin, myoglobin) to an electronic device using 11-MUA (11-mercaptoundecanoic acid) as the chemical linker. The immobilization of the 4 different self-assembled protein layers on a Au substrate via 11-MUA were confirmed by Raman spectroscopy and atomic force microscopy (AFM). The redox properties of these 4 different protein layers immobilized onto Au surface were assessed by cyclic voltammetry (CV). In addition, their memory functions were verified by chronoamperometry (CA). Based on these results, we demonstrated that a multi-bit biomemory concept could be realized using various metalloproteins as active materials. Copyright© 2011 American Scientific Publishers All rights reserved.

关键词： Cyclic voltammetry

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Single-molecule fluorescence study on the phase shift and phase locking of RecA nucleoprotein filament

Single-molecule fluorescence study on the phase shift and ph...

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第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Sung Hyun Kim TakKyoon Ahn Chirlmin Joo Doseok Kim Interdisciplinary Program of Integrated Biotechnology Sogang UniversitySeoulRepublic of Korea Department of Physics Sogang UniversitySeoulRepublic of Korea Kavli Institute of NanoScience and Department of BioNanoScience Delft University of TechnologyDelftThe Netherlands

RecA protein lies at the core of DNA repair *** forms a helical structured filament on as single-strand DNA(ssDNA) appears as an intermediate of homologous *** the recent RecA crystal structure with embedded ssDNA,a RecA monomer binds to a group of three nucleotides and generates a gap in ***,there are three possible phases in the binding of RecA according to the relative position

关键词： RecA single molecule FRET DNA repair

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Improved histogram based ghost removal in exposure fusion for high dynamic range images

Improved histogram based ghost removal in exposure fusion fo...

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IEEE International Symposium on Consumer Electronics (ISCE)

作者： D.-K. Lee R.-H. Park S. Chang Department of Electronic Engineering School of Engineering Sogang University Seoul South Korea Interdisciplinary Program of Integrated Biotechnology Sogang University Seoul Korea Samsung Electronics Co. Ltd. Suwon Gyeonggi-do Korea

关键词： Imaging Pixel Dynamic range Histograms Jacobian matrices Simulation Fuses

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Evaluation of flow estimation methods for 3D color Doppler imaging

Evaluation of flow estimation methods for 3D color Doppler i...

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IEEE International Ultrasonics Symposium

作者： Yu, Jaesok Lee, Wooyoul Song, Tai-Kyong Yoo, Yang Mo Department of Electronic Engineering Sogang University Seoul Korea Republic of Interdisciplinary Program of Integrated Biotechnology Sogang University Seoul Korea Republic of

ISBN: (纸本)9781457703829

In 3D ultrasound color Doppler imaging (CDI), similar to 2D CDI, 8-12 pulsetransmissions (ensembles, E) per each scanline are used for clutter rejectionand flow estimation, leading to a low volume acquisition rate. This rate couldbe improved by using a small number of ensembles (e.g., E=4). However, theimpact of the use of a small ensemble size on clutter rejection and flowestimation must be investigated. In this paper, we have evaluated three flowestimation methods: autoregression (AR), eigendecomposition (ED), andautocorrelation (AC) for a small size ensemble (E=4). To compare the performanceof three methods, raw radio-frequency (RF) data were acquired from the Dopplerflow phantom (RMI-1425A, Gammex Inc., USA) by using a commercial ultrasoundmachine (Accuvix V10, Medison Corp., Korea) equipped with a research *** the phantom experiment, the AR estimator exhibited a significantimprovement in area under receive operating characteristic curves compared to ACand ED methods (i.e., 0.97 ± 0.02 vs. 0.86 ± 0.07 and 0.83± 0.05, respectively). There is no statistically significant differencebetween the AC and ED methods (p>0.24). These results indicate that the ARestimator would be suitable for flow estimation in 3D CDI with a low ensemblesize. © 2010 IEEE.

关键词： Phantoms

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Biomemory device composed of recombinant azurin

Biomemory device composed of recombinant azurin

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2010 10th IEEE Conference on Nanotechnology, NANO 2010

作者： Choi, Jeong-Woo Department of Chemical and Biomolecular Engineering Interdisciplinary Program of Integrated Biotechnology Sogang University #1 Shinsu-Dong Mapo-Gu Seoul 121-742 Korea Republic of

ISBN: (纸本)9781424470334

Various electronic devices consisting of biomolecules have been developed in the fields of clinic, pharmaceutical, and environment. There are several efforts to make a new-type electronic device for altering a conventional silicon-based device that has several physical and technical limitations. A bioelectronic device based on mimicking of natural electron transfer phenomenon has been investigated to suggest a new-type electronic device. Here, we introduced device concept, fabrication method, and working principles of the biomemory device consisting of recombinant azurin. ©2010 IEEE.

关键词： Thermoelectric equipment

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Advances in quantitative proteomics

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Frontiers in Biology 2010年第3期5卷 195-203页

作者： Yuguang WANG Haiying LI Sixue CHEN College of Life Sciences Heilongjiang UniversityHarbin 150080China Department of Biology Genetics InstituteThe Plant Molecular and Cellular Biology ProgramInterdisciplinary Center for Biotechnology ResearchUniversity of FloridaGainesvilleFL 32611USA

Large-scale protein quantification has become a major proteomics application in many areas of biological and medical *** the past years,different techniques have been developed,including gel-based such as differential in-gel electrophoresis(DIGE)and liquid chromatography-based such as isotope labeling and labelfree *** quantitative proteomics tools hold significant promise for biomarker discovery,diagnostic and therapeutic *** are also important for research in functional genomics and systems biology towards basic understanding of molecular networks and pathway *** this review,we summarize current technologies in quantitative proteomics and discuss recent applications of the technologies.

关键词： two dimensional gel liquid chromatographymass spectrometry(LC-MS) stable isotope quantification application

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Proteomics characteristics of rice leaves in response to environmental factors

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Frontiers in Biology 2010年第3期5卷 246-254页

作者： Sining KANG Sixue CHEN Shaojun DAI College of Life Sciences Key Laboratory of Forestry Tree Genetics Improvement and BiotechnologyMinistry of EducationNortheast Forestry UniversityHarbin 150040China Department of Biology Genetics InstitutePlant Molecular and Cellular Biology ProgramInterdisciplinary Center for Biotechnology ResearchUniversity of FloridaGainesvilleFL 32610USA

Rice is an important food crop *** productivity has been influenced by various abiotic and biotic factors including temperature,drought,salt,microbe,ozone,hormone and *** responses of plants to stress are regulated by multiple signaling pathways,and the mechanisms of leaf growth and development in response to stress remain unclear to ***,proteomics studies have provided new evidence for better understanding the *** proteins in response to different stress conditions are mainly involved in photosynthesis,signal transduction,transcription,protein synthesis and destination,defense response,cytoskeleton,energy,cell wall and other *** addition,some stress type-specific proteins have been identified,such as small heat shock proteins under temperature stress,S-like RNase homolog and actin depolymerizing factor under drought stress,ascorbate peroxidase and lipid peroxidation under salt stress,probenazole-inducible protein and rice pathogenesis-related proteins under blast *** of the proteins including ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO),molecular chaperones,antioxidases and S-adenosylmethionine synthetase play very important roles in *** paper reviews the proteomic characterization of rice leaves in response to various environmental factors.

关键词： rice leaf proteomics environmental factor

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Ultrasound harmonic imaging using nonlinear chirp for cardiac imaging

Ultrasound harmonic imaging using nonlinear chirp for cardia...

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IEEE International Ultrasonics Symposium

作者： Song, Hyun-Jae Song, Jaehee Chang, Jin Ho Song, Tai-Kyong Department of Electronic Engineering Sogang University Seoul Korea Republic of Interdisciplinary Program of Integrated Biotechnology Sogang University Seoul Korea Republic of Sogang Institute of Advanced Technology Sogang University Seoul Korea Republic of

ISBN: (纸本)9781457703829

Coded excitation techniques have been used to improve a signal-to-noiseratio (SNR) in tissue harmonic imaging. However, a poor separation betweenfundamental and target harmonic components causes the second harmonic signals tohave a high level of range sidelobes after compression. In terms of theseparation performance, pulse inversion (PI) is the best method and thusprovides the lowest level of range sidelobes compared to bandpass filtering(BPF) and power modulation. However, the PI requires two transmit steps, thusinevitably decreasing frame rate. This paper proposes a new coded excitationtechnique using a nonlinear chirp for tissue harmonic imaging, particularlycardiac imaging. Since the spectral overlap between a transmitted nonlinearchirp and its second harmonic counterpart is less than the case of linear chirp,a simple BPF can be used for the separation so as to provide the range sidelobelevels comparable to those produced by PI without a loss of frame rate. In theexperiments, the highest level of the sidelobes of the nonlinear chirp was 40.59dB, which was comparable to the value from PI, i.e., 39.84 dB. © 2010IEEE.

关键词： Tissue

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Adaptive quadrature demodulation for ultrasound tissue harmonic imaging

Adaptive quadrature demodulation for ultrasound tissue harmo...

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2010 IEEE International Ultrasonics Symposium, IUS 2010

作者： Lee, Da-Young Yoon, Changhan Yoo, Yangmo Song, Tai-Kyong Chang, Jin Ho Department of Electronic Engineering Sogang University Seoul Korea Republic of Interdisciplinary Program of Integrated Biotechnology Sogang University Seoul Korea Republic of Sogang Institute of Advanced Technology Sogang University Seoul Korea Republic of

ISBN: (纸本)9781457703829

Frequency dependent attenuation of ultrasound causes the center frequencyof received echoes to be downshifted along depth. This is particularly severe ona harmonic component rather than a fundamental one because higher ultrasoundfrequency experiences higher attenuation. The different degrees of downshiftingalong with ultrasound frequency should be considered when quadraturedemodulation (QDM) is carried out to secure the best signal-to-noise ratio(SNR). In the conventional second harmonic imaging, however, a constantattenuation coefficient is typically assumed for estimating the center frequencyof the harmonic components in QDM. This assumption may not be valid becausethere is the attenuation variation in an imaging area. To obtain the best SNR,therefore, this paper proposes an automatic center frequency estimator based onautoregressive (AR) modeling for the ultrasound second-harmonic tissue *** the proposed method, the estimated center frequency of a second-harmoniccomponent is directly used for dynamic QDM and dynamic low-pass filtering (LPF).The performance of the proposed method was evaluated by Field II simulation andin-vivo experiments. The experimental results demonstrated that the proposedmethod is capable of providing higher contrast resolution while reducinghigh-frequency noise under visual examination due to the improvement of SNR.© 2010 IEEE.

关键词： Signal to noise ratio

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