检索结果-内蒙古大学图书馆

Interleukin-1 receptor 1 deficiency worsens hepatocellular carcinoma, while gemcitabine treatment alleviates the hepatocellular carcinoma-induced increase in intra-hepatic immune cells

引用

Journal of gastroenterology and hepatology 2024年第10期39卷 2208-2218页

作者： Chia-Sheng Chu Hsiao-Ping Chen Pin-Hung Lin Chi-Chen Cheng Ho-Yu Kuo Pei-Han Fan Wei-Hao Peng Li-Ling Wu Ph.D. Program of Interdisciplinary Medicine National Yang Ming Chiao Tung University Taipei Taiwan. Ever Health Clinic Taichung Taiwan. Health Innovation Center National Yang Ming Chiao Tung University Taipei Taiwan. Microbiota Research Center National Yang Ming Chiao Tung University Taipei Taiwan. Department of Microbiology National Taiwan University College of Medicine Taipei Taiwan. School of Medicine College of Medicine National Yang Ming Chiao Tung University Taipei Taiwan. Department and Institute of Physiology National Yang Ming Chiao Tung University Taipei Taiwan. School of Medicine Department of Medical Science National Tsing Hua University Hsinchu Taiwan. Institute of Biotechnology National Tsing Hua University Hsinchu Taiwan.

BACKGROUND AND AIM:Primary liver cancer, particularly hepatocellular carcinoma (HCC), represents a substantial global health challenge. Although immune checkpoint inhibitors are effective in HCC treatment, several patients still experience disease progression. Interleukin-1 (IL-1) regulates immunity and inflammation. We investigate the role of IL-1 in HCC development and progression and determine the potential therapeutic impact of gemcitabine in treating HCC. METHODS:Hydrodynamics-based transfection, employing the sleeping beauty transposase system, delivered surrogate tumor antigens, NRAS (NRAS proto-oncogene, GTPase), ShP53, and SB100 to C57BL/6 mice. A basic HCC mouse model was established. Pathogen-free animals were tested for serum and hepatotoxicity. The HCC prognosis was monitored using alanine aminotransferase and aspartate aminotransferase levels. Liver histology immunohistochemistry and mouse splenocyte/intra-hepatic immune cell flow cytometry were conducted. IL-1β levels in human and mouse serum were assessed. RESULTS:Interleukin-1β levels were elevated in patients with HCC compared with those in non-HCC controls. Hepatic IL-1β levels were higher in HCC mouse models than those in non-HCC mice, suggesting localized hepatic inflammation. IL-1 receptor type 1 (IL-1R1) knockout (IL-1R1) mice exhibited less severe HCC progression than that in wild-type mice, despite the high intra-hepatic IL-1β concentration. IL-1R1 mice exhibited increased hepatic levels of myeloid-derived suppressor cells and regulatory T cells, which may exacerbate HCC. Gemcitabine significantly reduced the HCC tumor burden, improved liver conditions, and increased survival rates in HCC mouse models. Gemcitabine reduced the hepatic levels of myeloid-derived suppressor cells and regulatory T cells, potentially alleviating immune suppression in the liver. CONCLUSIONS:Targeting IL-1 or combining gemcitabine with immunotherapy is a promising approach for treating advanced-stage HCC.

关键词： IL‐1R1 T regulatory cells gemcitabine hepatocellular carcinoma myeloid‐derived suppressor cells

来源：评论

学校读者我要写书评

暂无评论

Elongation factor-G1A identified as a novel effector protein translocated into cells and a key modulator of Pseudomonas aeruginosa physiology and host cellular responses

引用

Microbial Pathogenesis 2025年 205卷 107720页

作者： Lee, Yeji Jin, Yongxin Wu, Weihui Ha, Un-Hwan Department of Biotechnology and Bioinformatics Korea University Sejong 30019 South Korea State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education Department of Microbiology Nankai University Tianjin 300071 China Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology Korea University Sejong 30019 South Korea

Pseudomonas aeruginosa has two closely related elongation factors, EF-G1A and EF-G1B, which share 90 % sequence similarity. Despite their high sequence homology, the role of EF-G in P. aeruginosa pathogenesis remains not fully understood. In our study, we found that compared to EF-G1B, EF-G1A expression reduced bacterial growth and twitching motility, while increasing swimming motility. Notably, EF-G1A was translocated into host cells in a T6SS-dependent manner. This translocation was significantly reduced, though not completely abolished, in strains with mutations in both the T6SS spike protein VgrG1a and the tube protein Hcp1, suggesting that while EF-G1A translocation is influenced by T6SS, additional components are also involved. Moreover, EF-G1A expression reduced T3SS-mediated morphological alterations, as evidenced by the downregulation of T3SS effectors such as ExoS and ExoT. EF-G1A was also found to suppress activation of the NF-κB signaling pathway, leading to decreased production of inflammatory cytokines including IL-6, IL-8, and TNFα. These findings highlight EF-G1A as a key modulator, affecting both P. aeruginosa physiology and host cellular responses, thereby providing new insights into the complex role of EF-G1A in bacterial pathogenesis. © 2025 Elsevier Ltd

关键词： Elongation factor-G1A Pseudomonas aeruginosa T3SS T6SS Translocation

来源：评论

学校读者我要写书评

暂无评论

Mancozeb Concentration Allowed in Brazilian Drinking Water Causes Oxidative Stress and Bioenergetics Impairments in Embryonic Zebrafish

SSRN

引用

SSRN 2023年

作者： Leandro, Luana Paganotto Mariano, Maria Vitória Takemura Gomes, Karen Kich dos Santos, Ana Beatriz dos Anjos, Jaciana Sousa de Carvalho, Nelson Rodrigues Nunes, Mauro Eugênio Medina Farina, Marcelo Posser, Thais Franco, Jeferson Luis Oxidative Stress and Cell Signaling Research Group Interdisciplinary Center for Biotechnology Research CIPBIOTEC Federal University of Pampa São Gabriel RS 97307-020 Brazil Department of Molecular Biology and Biochemistry. Graduate Program in Biological Sciences: Toxicological Biochemistry Federal University of Santa Maria 1000 Roraima Avenue RS Santa Maria97105-900 Brazil Farroupilha Federal Institute Campus Santo Angelo Rs-218 RS Indubrás 98806-700 Brazil Department of Genetics and Exercise Metabolism. Graduate Program in Molecular Biology Federal University of Sao Paulo 1500 Sena Madureira St SP São Paulo04021-001 Brazil Department of Biochemistry Center for Biological Sciences Federal University of Santa Catarina SC Florianópolis88040-900 Brazil

Mancozeb (MZ), a fungicide containing manganese/zinc, is the 3rd most used agrochemical in Brazil and its toxicity has been reported in the literature. A recent update of the Brazilian legislation allowed a concentration of 8 µg/L of MZ in drinking water (Ordinance GM/MS No. 888, of May 4, 2021), but there are no studies that prove the safety of this concentration for non-target organisms. To investigate this, we used zebrafish (Danio rerio) embryos with 4 hpf (hours post-fertilization) exposed to MZ at the concentration permitted by law and also to slightly higher but sublethal concentrations (24, 72, and 180 µg/L) in parallel to a control group. Survival, spontaneous movements, heart rate, hatching rate, body axis distortion, total body length, total yolk sac area, and total eye area were evaluated as morphophysiological markers of toxicity. In addition, biochemical biomarkers including levels of reactive oxygen species (ROS), lipid peroxidation, non-protein thiols (NPSH,), and mitochondrial bioenergetics parameters were measured. As a result, MZ exposure starting from concentrations higher than 8 µg/L, was capable of impairing morphophysiological markers. Furthermore, ROS increased at all concentrations tested. Mitochondrial bioenergetics function appeared to be the most sensitive marker of MZ embryotoxicity in our model, in which decreased complex I activity was observable at concentrations of 8 and 180 µg/L. According to this evidence, we can infer that the MZ concentration permitted in potable water by the Brazilian environmental legislation, is not safe for non-target organisms, demonstrating this fungicide as a strong embryotoxic agent. © 2023, The Authors. All rights reserved.

关键词： Toxicity

来源：评论

学校读者我要写书评

暂无评论

Cross-modal attention for MRI and ultrasound volume registration

arXiv

引用

arXiv 2021年

作者： Song, Xinrui Guo, Hengtao Xu, Xuanang Chao, Hanqing Xu, Sheng Turkbey, Baris Wood, Bradford J. Wang, Ge Yan, Pingkun Department of Biomedical Engineering Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute TroyNY12180 United States Center for Interventional Oncology Radiology and Imaging Sciences National Institutes of Health BethesdaMD20892 United States Molecular Imaging Program National Cancer Institute National Institutes of Health BethesdaMD20892 United States

Prostate cancer biopsy benefits from accurate fusion of transrectal ultrasound (TRUS) and magnetic resonance (MR) images. In the past few years, convolutional neural networks (CNNs) have been proved powerful in extracting image features crucial for image registration. However, challenging applications and recent advances in computer vision suggest that CNNs are quite limited in its ability to understand spatial correspondence between features, a task in which the self-attention mechanism excels. This paper aims to develop a self-attention mechanism specifically for cross-modal image registration. Our proposed crossmodal attention block effectively maps each of the features in one volume to all features in the corresponding volume. Our experimental results demonstrate that a CNN network designed with the cross-modal attention block embedded outperforms an advanced CNN network 10 times of its size. We also incorporated visualization techniques to improve the interpretability of our network. The source code of our work is available at https://***/DIAL-RPI/Attention-Reg. Copyright © 2021, The Authors. All rights reserved.

关键词： Urology

来源：评论

学校读者我要写书评

暂无评论

Drivers of Planktonic Chlorophyll a in Pampean Shallow Lakes: A Regional Approach

SSRN

引用

SSRN 2022年

作者： Sánchez, Maria Laura Izaguirre, Irina Zagarese, Horacio Schiaffino, María Romina Castro Berman, Manuel Lagomarsino, Leonardo Chaparro, Griselda Baliña, Sofia Vera, María Solange Cheruvelil, Kendra S. Ciudad Autónoma de Buenos Aires Argentina Argentina Argentina Departamento de Ciencias Básicas Experimentales Universidad Nacional del Noroeste de la Provincia de Buenos Aires Junín6000 Argentina UNNOBA UNSAdA-CONICET Argentina Center for Biotechnology & Interdisciplinary Studies Rensselaer Polytechnic Institute TroyNY12180 United States Department of Fisheries and Wildlife Lyman Briggs College Ecology and Evolutionary Biology Program Michigan State University East Lansing United States

Although agricultural intensification has been linked with the increment of lake eutrophication and shallow lake research has demonstrated the importance of submerged macrophytes for maintaining water clarity, less is known about the role of macrophytes and the effects of agriculture on shallow lakes of the global south. Shallow lakes in the Pampean region of Argentina are subjected to high anthropic pressure and are classified as eutrophic and hypertrophic and, in spite of most of them are turbid and dominated by phytoplankton biomass, some remain in a clear-vegetated regime with profuse submerged macrophytes. We studied the potential drivers of phytoplankton biomass (estimated as Chlorophyll- a - Chl a ) by applying a regional approach and a model selection process for a dataset of 58 shallow lakes that represent the variability of the Pampean region. For the 58 lakes, the presence of submerged macrophytes, total nitrogen, and nearby agriculture of each lake were the main drivers of Chl a with negative, positive and positive coefficient values, respectively. Moreover, a high proportion of the variance in this dataset was explained by the regional location of each lake (hydrographic systems). For lakes with macrophytes (N=8), Chl a exhibited a positive relationship with total phosphorus, whereas for lakes without macrophytes (N=50) Chl a showed a positive relationship with nearby agricultural development. Our regional approach highlighted the importance of submerged macrophytes in shaping phytoplankton biomass in Pampean shallow lakes. Our results support the idea that the conservation of submerged macrophytes, as well as the control of agriculture in the riparian zones of lakes, will help to stabilize the shallow lakes in clear regime, even in regions highly impacted by agriculture and in lakes under eutrophic conditions. © 2022, The Authors. All rights reserved.

关键词： Agriculture

来源：评论

学校读者我要写书评

暂无评论

Phage display-derived alpaca nanobodies as potential therapeutics for Naja atra snake envenomation

引用

Applied and Environmental Microbiology 2024年第8期90卷 e0012124页

作者： Wang, Wei-Chu Chang, Jungshan Lee, Chi-Hsin Chiang, Yu-Wei Leu, Sy-Jye Mao, Yan-Chiao Chiang, Jen-Ron Yang, Chun-Kai Wu, Chao-Jung Yang, Yi-Yuan Ph.D. Program in Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei Taiwan International Master/Ph.D. Program in Medicine College of Medicine Taipei Medical University Taipei Taiwan International Ph.D. Program for Cell Therapy and Regeneration Medicine College of Medicine Taipei Medical University Taipei Taiwan School of Medical Laboratory Science and Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan Core Laboratory of Antibody Generation and Research Taipei Medical University Taipei Taiwan Department of Medical Research Taipei Veterans General Hospital Taipei Taiwan Department of Biology and Anatomy National Defense Medical Center Taipei Taiwan Foundation for Poison Control Taipei Taiwan Department of Microbiology and Immunology School of Medicine College of Medicine Taipei Medical University Taipei Taiwan School of Medicine National Defense Medical Centre Taipei Taiwan Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung Taiwan Division of Clinical Toxicology Department of Emergency Medicine Taichung Veterans General Hospital Taichung Taiwan Bioproduction Plants National Institute of Infectious Diseases and Vaccinology National Health Research Institutes Miaoli Taiwan Interdisciplinary Research on Ecology and Sustainability National Dong Hwa University Hualien Taiwan

Naja atra, the Chinese cobra, is a major cause of snake envenomation in Asia, causing hundreds of thousands of clinical incidents annually. The current treatment, horse serum-derived antivenom, has unpredictable side effects and presents manufacturing challenges. This study focused on developing new-generation snake venom antidotes by using microbial phage display technology to derive nanobodies from an alpaca immunized with attenuated N. atra venom. Following confirmation of the immune response in the alpaca, we amplified VHH genes from isolated peripheral blood mononuclear cells and constructed a phage display VHH library of 1.0 × 107 transformants. After four rounds of biopanning, the enriched phages exhibited increased binding activity to N. atra venom. Four nanobody clones with high binding affinities were selected: aNAH1, aNAH6, aNAH7, and aNAH9. Specificity testing against venom from various snake species, including two Southeast Asian cobra species, revealed nanobodies specific to the genus Naja. An in vivo mouse venom neutralization assay demonstrated that all nanobodies prolonged mouse survival and aNAH6 protected 66.6% of the mice from the lethal dosage. These findings highlight the potential of phage display-derived nanobodies as valuable antidotes for N. atra venom, laying the groundwork for future applications in snakebite treatment. Copyright © 2024 American Society for Microbiology. All Rights Reserved.

关键词： Mammals

来源：评论

学校读者我要写书评

暂无评论

Author Correction: Senotherapeutic peptide treatment reduces biological age and senescence burden in human skin models

引用

npj aging 2024年第1期10卷 14页

作者： Alessandra Zonari Lear E Brace Kallie Al-Katib William F Porto Daniel Foyt Mylieneth Guiang Edgar Andres Ochoa Cruz Bailey Marshall Melissa Gentz Gabriela Rapozo Guimarães Octavio L Franco Carolina R Oliveira Mariana Boroni Juliana L Carvalho OneSkin Inc. San Francisco CA USA. alessandra@oneskin.co. OneSkin Inc. San Francisco CA USA. Genomic Sciences and Biotechnology Program Catholic University of Brasilia Brasília 70790-160 DF Brazil. Porto Reports Brasília 72236-011 DF Brazil. Bioinformatics and Computational Biology Lab Brazilian National Cancer Institute (INCA) Rio de Janeiro 20231-050 RJ Brazil. Centre of Proteomic Analyses and Biochemistry Genomic Sciences and Biotechnology Program Catholic University of Brasilia Brasilia 70790-160 DF Brazil. S-Inova Biotech Biotechnology Program Catholic University Dom Bosco Campo Grande 79117-010 MS Brazil. Molecular Pathology Program University of Brasilia Brasilia 70.910-900 DF Brazil. Interdisciplinary Biosciences Laboratory Faculty of Medicine University of Brasília Brasília 70.910-900 DF Brazil.

来源：评论

学校读者我要写书评

暂无评论

Sequential Wet Extraction of Phosphorus from Sewage Sludge Using Alum Sludge: Reassessing the Aluminium-Phosphorus Speciation Using Experimental and Simulation Approach

SSRN

引用

SSRN 2022年

作者： Tiwari, Satya Brat Hooper, Thomas J.N. Veksha, Andrei Chan, Wei Ping Fei, Xunchang Liu, Wen Lisak, Grzegorz Lim, Teik-Thye School of Civil and Environmental Engineering Nanyang Technological University 50 Nanyang Avenue 639798 Singapore Nanyang Environment and Water Research Institute Nanyang Technological University 1 Cleantech Loop CleanTech One 637141 Singapore Interdisciplinary Graduate Program Nanyang Technological University Singapore Centre of High Field NMR Spectroscopy and Imaging Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University 21 Nanyang Link 637371 Singapore School of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University 62 Nanyang Drive 637459 Singapore

Sewage sludge (SS) is a reclaimable phosphorus (P) source and is a potential alternative to the natural phosphate mineral. This study explored the alkaline leaching of P from alum sludge (AS)-amended dry SS, with or without the acidic pretreatment. The highest orthophosphate (ortho-P) alkaline recovery was 74% for the acid-pretreated SS+AS mix. Amending SS with AS averted the loss of ortho-P during the acidic pretreatment by 59% compared to the reference case (unamended SS). Standards Measurements and Testing (SMT) analysis revealed apatite to non-apatite inorganic phosphorus conversion during the acidic pretreatment, especially in the amended SS. This conversion was due to the dissolution of Ca-P and adsorption of dissolved ortho-P on Al(OH)3 during the acidic pretreatment. Visual MINTEQ modelling, solid-state NMR (31P, 27Al and 31P-1H CP-HETCOR) and Al 2p XPS data indicated the high abundance of Al(OH)3(s), which enhanced the ortho-P adsorption. There was no formation of AlPO4, possibly because the acid-sludge mixture reached the metastable equilibrium and not the true equilibrium during the acidic pretreatment. 31P liquid NMR, SEM, FTIR and XRD provided additional insight into the effects of acidic pretreatment on sludges. Overall, Ca-P to Al-P conversion enhanced the alkaline ortho-P recovery by about 12% compared to the reference case (unamended + non-pretreated). This study demonstrated that P leaching efficiency from SS can be improved (with appropriate process modifications) irrespective of the intrinsic property of SS. © 2022, The Authors. All rights reserved.

关键词： Sewage sludge

来源：评论

学校读者我要写书评

暂无评论

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

引用

Insect Science 2019年第5期26卷 831-842页

作者： Sasiprapa Krongdang Jay D.Evans Yanping Chen Wannapha Mookhploy Panuwan Chantawannakul 1Bee Protection Laboratory Department of BiologyFaculty of ScienceChiang Mai UniversityChiang MaiThailand Interdisciplinary Program in Biotechnology Graduate SchoolChiang Mai UniversityChiang MaiThailand USDA-ARS Bee Research LaboratoryBeltsvilleMarylandUSA Environmental Science Research Center Faculty of ScienceChiang Mai UniversityChiang MaiThailand

American foulbrood (AFB) disease is caused by Paenibacillus larvae. Currently, this pathogen is widespread in the European honey bee— Apis mellifera. However, little is known about infectivity and pathogenicity of P. lan'ae in the Asiatic cavity-nesting honey bees, Apis cerana. Moreover, comparative knowledge of P. larvae infectivity and pathogenicity between both honey bee species is scarce. In this study, we examined susceptibility, larval mortality, survival rate and expression of genes encoding antimicrobial peptides (AMPs) including defensin, apidaecin, abaecin, and hymenoptaecin in A. mellifera and A. cerana when infected with P. larvae. Our results showed similar effects of P. larvae on the survival rate and patterns of AMP gene expression in both honey bee species when bee larvae are infected with spores at the median lethal concentration (LC5 0 ) for A. mellifera. All AMPs of infected bee larvae showed significant upregulation compared with noninfected bee larvae in both honey bee species. However, larvae of A. cerana were more susceptible than A. mellifera when the same larval ages and spore concentration of P. larvae were used. It also appears that A. cerana showed higher levels of AMP expression than A. mellifera. This research provides the first evidence of survival rate, LC50 and immune response profiles of Asian honey bees, A. cerana, when infected by P. larvae in comparison with the European honey bee, A. mellifera.

关键词： Apis cerana Apis mellifera honey bee immune gene Paenibacillus larvae susceptibility

来源：评论

学校读者我要写书评

暂无评论

Discovery of amino acid-conjugated dimethylcardamonin analogues as potent anti-cervical cancer agents on SiHa cells targeting p53 signalling pathway

引用

Biomedicine and Pharmacotherapy 2024年 181卷 117705-117705页

作者： Khamto, Nopawit Utama, Kraikrit Chawapun, Pornthip Siriphong, Sadanon Tateing, Suriya Duangdesh, Sarocha Sangthong, Padchanee Chomsri, Ni-orn Meepowpan, Puttinan Department of Chemistry Faculty of Science Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Multidisciplinary and Interdisciplinary School Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Office of Research Administration Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Program in Biotechnology Multidisciplinary and Interdisciplinary School Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Department of Plant and Soil Sciences Faculty of Agriculture Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Agricultural Technology Research Institute (ATRI) Rajamangala University of Technology Lanna 202 Pichai District Lampang 52100 Thailand Center of Excellence in Materials Science and Technology Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand Center of Excellence for Innovation in Chemistry (PERCH-CIC) Faculty of Science Chiang Mai University 239 Huay Kaew Road Chiang Mai 50200 Thailand

DMC (1) is a phytochemical found in the seeds of Syzygium nervosum, exhibiting anticancer activity in various cells through multiple pathways. Herein, the bioactivity of DMC (1) was enhanced by chemical modification through esterification, attaching fatty acid and amino acid moieties to yield 27 semi-synthetic derivatives. These compounds were evaluated for their in vitro cytotoxicity against three main types of cervical cancer cells, including SiHa, HeLa, and C-33A. As a result, the amino acid DMC derivative, 4´-(L-tyrosinyloxy)-DMC (7j), exhibited potent cytotoxicity against SiHa cells, which was approximately two-fold greater than that of 1. Further investigation into the mechanism of action of 7j was conducted, revealing its ability to induce cell cycle arrest and apoptosis. Gene expression analysis showed the downregulation of CDK2 and upregulation of the BAX/BCL2 ratio. Atomistic insight was studied on HPV 16 E6 via molecular dynamics simulation, revealing key interactions between tyrosinyl portion and C51 residue. © 2024 The Authors

关键词： Anticancer Cervical cancer Chalcone Structural modification

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：