Binocular indirect ophthalmoscope (BIO) provides a wider view of fundus with stereopsis contrary to the direct one. Proposed system is composed of portable BIO and 3D viewing unit. The illumination unit of BIO utilize...
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A sophisticatedly designed polyelectrolyte diode was fabricated on a microchip and exhibited well-defined nonlinear rectifying behavior. Multiple polyelectrolyte diodes were integrated on a microchip to produce a vari...
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This work reports a new method to partially coat a film of our interest on a microbead with a thin gold shell, briefly 'gold ball', using wireless electrochemistry. The key point of this method is to control t...
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This paper reports the decoding strategy of surface-enhanced Raman scattering (SERS) signals from microspheres with Au shell structures, or gold balls, with Raman-tags in a microfluidic channel. On the surface of the ...
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Authentication using finger vein pattern has substantial advantage than other biometrics. Because human vein patterns are hidden inside the skin and tissue, it is hard to forge vein structure. But conventional system ...
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A portable flow cytometer with simultaneous detection of impedance and fluorescence was constructed incorporating polyelectrolytic gel electrodes (PGE) based impedance measurement and fluorescence measurement with int...
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An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent mi...
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An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent micro- and nanospheres each conjugated with different proteins are size-dependently self-assembled (SDSA) onto these nanometer wells that were created on the polymethyl methacrylate (PMMA) substrate by electron beam lithography (EBL). Particles of different diameters are added serially, and electrostatically attached to the corresponding wells through electrostatic attraction between the carboxylic groups of the spheres and p-doped silicon substrate underneath the PMMA layer. Target detection utilizes fluorescence resonance energy transfer (FRET) from fluorescent beads to target (mouse immunoglobulin G = mIgG or Octamer-4 = Oct4) and its antibody bound on the beads. The 180 nm blue beads are conjugated with mIgG to capture anti-mIgG-FITC. The 50 nm green and 100 nm yellow-green beads are conjugated with anti-Oct4 to capture Oct4 peptides; where the secondary anti-Oct4 tagged with phycoerythrin via F(ab)2 fragment is then added to function as an indicator of Oct4 detection. These protein-conjugated particles are added serially from the largest to the smallest and the particles are successfully self-assembled to the respective nanometer wells to achieve size-dependent self-assembly. FRET signals are detected through fluorescence and confocal microscopes, and further confirmed by Fluorolog3 spectrofluorometer. Therefore, SDSA is a valuable approach for the fabrication of multiple components array; and FRET is a useful biorecognition technique for the detection of mIgG, Oct4 or other targets of interest.
The design of a composite biodegradable scaffold for tissue engineering was the main focus of this investigation. The composite material was made from a highly porous mesh of poly(epsiloncaprolactone) (PCL) nanofibers...
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