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Biomechanical Comparison of Double 2.3-mm Headless Cannulated Self-Compression Screws and Single 3.5-mm Cortical Screw in Lag Fashion in a Canine Sacroiliac Luxation Model: A Small Dog Cadaveric Study

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Veterinary and Comparative Orthopaedics and Traumatology 2023年第1期37卷 013-022页

作者： AhRan Kang Haebeom Lee Arim Lee Yoonho Roh Bokyun Sim Jaemin Jeong College of Veterinary Medicine Chungnam National University Yuseong-gu Daejeon Republic of Korea Division of Animal Surgery Department of Clinical Veterinary Medicine Vetsuisse Faculty University of Bern Bern Switzerland Graduate Program of Biomedical Engineering Yonsei University Seoul Republic of Korea

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The Investigation of Hybrid and Layered Ha/Ta2o5 Sol-Gel Composite Coating on Az91 Mg Alloy

SSRN

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SSRN 2023年

作者： Albayrak, Sevda Gül, Canser Emin, Nuray Gökmen, Uğur Karakoç, Halil Uzun, Arif Çinici, Hanifi Gazi University Technology Faculty Department of Metallurgical and Materials Engineering Ankara06500 Turkey Manisa Celal Bayar University Department of Metallurgical and Materials Engineering Manisa45140 Turkey Kastamonu University Department of Biomedical Engineering Kastamonu37150 Turkey Hacettepe University Department of Mechanical Program Ankara06935 Turkey Kastamonu University Department of Mechanical Engineering Kastamonu37150 Turkey

Corrosion and bacterial behaviour of amorphous tantalum oxide (Ta2O5) and hydroxyapatite (HA) were studied by sol-gel coating on AZ91 Mg alloy in this study. Investigating the potential of AZ91 Mg alloy for permanent prosthesis applications is the main motivation of this study. Ca(NO3)2.4H2O-P2O5 and Ta(OC2H5)5 chemical precursors were used for coating HA and Ta2O5 on AZ91, respectively. The coatings were deposited on the surfaces in layered and hybrid forms. Primarily the structural and morphological evaluations of the coatings were carried out by XRD, FTIR and SEM/EDS analyses, in experimental studies. Antibacterial activities were evaluated according to the interaction of the samples with gram-positive, Staphylococcus Aureus(S. Aureus) and gram-negative, Escherichia Coli (E. Coli) bacterial strains. Furthermore, linear scratch tests were done to find out the mechanical properties of the coatings and the interfacial connections with the substrate. The electrochemical corrosion tests were conducted by using Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle's Medium (DMEM) and 10% Fetal Bovine Serum (FBS) + DMEM mediums. Eventually, the aluminium release of the samples in DMEM was followed for 28 days with inductively coupled plasma emission spectroscopy (ICP-OES). As a result of the study, it was seen that the amorphous Ta2O5 coating improved the antibacterial property and positively affected the corrosion and scratch test results when coated with HA. © 2023, The Authors. All rights reserved.

关键词： Corrosion resistance

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Systematic analysis of multi-omics data reveals component-specific blood-based biomarkers for Parkinson’s disease

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Translational Medicine Communications 2024年第1期9卷 1-21页

作者： Tng, Teddy J. W. Wong, Barbara Wing Yan Sim, Esther H. Y. Tan, Eng King Goh, Wilson W. B. Lim, Kah-Leong Lee Kong Chian School of Medicine Nanyang Technological University Singapore Singapore School of Biological Sciences Nanyang Technological University Singapore Singapore Interdisciplinary Graduate Programme (IGP-Neuroscience) Nanyang Technological University Singapore Singapore School of Biomedical Sciences Faculty of Medicine Li Ka Shing The University of Hong Kong Hong Kong China School of Chemical and Biomedical Engineering Nanyang Technological University Singapore Singapore National Neuroscience Institute Singapore Singapore Center for Biomedical Informatics Nanyang Technological University Singapore Singapore

Parkinson’s disease (PD) is a prevalent neurodegenerative disorder affecting millions of elderly individuals worldwide. Clinically, PD is diagnosed based on the presentation of motoric symptoms. Other methods such as F-DOPA PET scan or α-Synuclein detection from the cerebral spinal fluid are either too expensive or invasive for routine use. Omics platforms such as transcriptomics, proteomics, and metabolomics may identify PD biomarkers from blood, which can reduce cost and increase efficiency. However, there are many biological moieties being measured and issues with false positives/negatives. It is also unknown which omics platform offers most useful information. Therefore, it is important to assess the reliability of these omics studies. Here, we shortlisted and analysed nearly 80 published reports across transcriptomics, proteomics and metabolomics in search of overlapping blood-based biomarkers for PD. The top biomarkers were reported across 29%, 42% and 12.5% of shortlisted papers in transcriptomics, proteomics and metabolomics respectively. These percentages increased to 42%, 60% and 50% accordingly when studies were grouped by specific blood subtypes for analysis, demonstrating the need for test kits to be blood-subtype specific. Following systematic analyses, we propose six novel PD biomarkers: two mRNAs (Whole blood, WB) – Arg1 and SNCA, two proteins (Plasma EV) – SNCA and APOA1, and two metabolites (WB) – 8-OHdG and uric acid for further validation. While these proposed biomarkers are useful, they are also snapshots, representing subsets of larger pathways of origin where the different omics levels corroborate. Indeed, identifying the interconnections across different biological layers can strengthen contextual reasoning, which in turn, would give rise to better quality biomarkers. Knowledge integration across the omics spectrum revealed consistent aberrations on the same neuroinflammation pathway, showcasing the value of integrative (i)-omics agreements f

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Importance of Standardizing Analytical Characterization Methodology for Improved Reliability of the Nanomedicine Literature

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Nano-Micro Letters 2022年第11期14卷 18-32页

作者： Shahriar Sharifi Nouf N.Mahmoud Elizabeth Voke Markita P.Landry Morteza Mahmoudi Department of Radiology and Precision Health Program Michigan State UniversityEast LansingMIUSA Faculty of Pharmacy Al-Zaytoonah University of JordanAmman 11733Jordan Department of Biomedical Sciences College of Health SciencesQU HealthQatar University2713 DohaQatar Department of Chemical and Biomolecular Engineering University of CaliforniaBerkeleyBerkeleyCAUSA Innovative Genomics Institute BerkeleyCAUSA California Institute for Quantitative Biosciences University of CaliforniaBerkeleyCAUSA Chan Zuckerberg Biohub San FranciscoCAUSA

Understanding the interaction between biological structures and nanoscale technologies,dubbed the nano-bio interface,is required for successful development of safe and efficient nanomedicine *** lack of a universal reporting system and decentralized methodologies for nanomaterial characterization have resulted in a low degree of reliability and reproducibility in the nanomedicine *** such,there is a strong need to establish a characterization system to support the reproducibility of nanoscience data particularly for studies seeking clinical ***,we discuss the existing key standards for addressing robust characterization of nanomaterials based on their intended use in medical devices or as *** also discuss the challenges surrounding implementation of such standard protocols and their implication for translation of nanotechnology into clinical ***,however,emphasize that practical implementation of standard protocols in experimental laboratories requires long-term planning through integration of stakeholders including institutions and funding agencies.

关键词： Characterization Nanomedicine Standard protocols Reproducibility Nanomedicine devices

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Cold atmospheric plasma-enabled platelet vesicle incorporated iron oxide nano-propellers for thrombolysis

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Materials Today Bio 2023年 23卷 100876页

作者： Jheng, Pei-Ru Chiang, Chia-Che Kang, Jiunn-Horng Fan, Yu-Jui Wu, Kevin C.-W. Chen, Yan-Ting Liang, Jia-Wei Bolouki, Nima Lee, Jyh-Wei Hsieh, Jang-Hsing Chuang, Er-Yuan Graduate Institute of Nanomedicine and Medical Engineering Graduate Institute of Biomedical Materials and Tissue Engineering Institute of Biomedical Optomechatronics International Ph.D. Program in Biomedical Engineering School of Biomedical Engineering College of Biomedical Engineering Professional Master Program in Artificial Intelligence in Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan Department of Physical Medicine and Rehabilitation Taipei Medical University Hospital Taipei Taiwan Institute of Biomedical Engineering & Nanomedicine National Health Research Institute Keyan Road Zhunan Miaoli City350 Taiwan Department of Chemical Engineering National Taiwan University No.1 Sec. 4 Roosevelt Rd Taipei10617 Taiwan Department of Plasma Physics and Technology Faculty of Science Masaryk University Brno Czech Republic Center for Plasma and Thin Film Technologies Ming Chi University of Technology New Taipei City Taiwan Department of Materials Engineering Ming Chi University of Technology New Taipei City Taiwan Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei11696 Taiwan

A new approach to treating vascular blockages has been developed to overcome the limitations of current thrombolytic therapies. This approach involves biosafety and multimodal plasma-derived theranostic platelet vesicle incorporating iron oxide constructed nano-propellers platformed technology that possesses fluorescent and magnetic features and manifold thrombus targeting modes. The platform is capable of being guided and visualized remotely to specifically target thrombi, and it can be activated using near-infrared phototherapy along with an actuated magnet for magnetotherapy. In a murine model of thrombus lesion, this proposed multimodal approach showed an approximately 80 % reduction in thrombus residues. Moreover, the new strategy not only improves thrombolysis but also boosts the rate of lysis, making it a promising candidate for time-sensitive thrombolytic therapy. © 2023 The Authors

关键词： Infrared devices

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Noggin expression in human nucleus pulposus cells: A comparative study of degenerative and traumatic intervertebral discs

Advanced Orthopaedics

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Advanced Orthopaedics 2025年 1卷 55-62页

作者： Shuimu Chen Sebastian F. Bigdon Sonja Häckel Zhen Li Christoph E. Albers Benjamin Gantenbein Department of Orthopaedic Surgery & Traumatology Inselspital Bern University Hospital Faculty of Medicine University of Bern Bern CH-3010 Switzerland Tissue Engineering for Orthopaedics & Mechanobiology (TOM) Bone & Joint Program Department for BioMedical Research (DBMR) Faculty of Medicine University of Bern Bern CH-3008 Switzerland Graduate School for Cellular and Biomedical Sciences (GCB) University of Bern Bern CH-3012 Switzerland Graduate School for Health Sciences (GHS) University of Bern CH-3012 Bern Switzerland AO Research Institute Davos Davos CH-7270 Switzerland

Low back pain (LBP) is a health issue that leads to considerable disability and socioeconomic burdens. The degeneration of the human intervertebral disc (IVD) is a key factor in the pathogenesis of LBP. During disc degeneration, the nucleus pulposus (NP), a critical component of IVD, undergoes significant alterations in structural composition and function. Recent studies have highlighted the importance of various genes in IVD health. Noggin has been described as an essential gene in nucleus pulposus cells (NPCs) and a central role in inhibiting osteogenesis. In this study, we conducted a comparative analysis of Noggin expression in human NPCs from degenerative and healthy IVD. Twenty-six IVD samples (nineteen from degeneration and seven from trauma) were collected to separate NPCs for RNA extraction. Noggin expression was quantified at the transcript level by qPCR to analyze the difference between degenerative and traumatic IVD. Results indicated that Noggin expression was lower in NPCs from degenerated IVD compared to trauma cases. The severity of IVD degeneration correlated with decreased Noggin levels. Traumatic males exhibited higher Noggin expression than traumatic females and those with IVD degeneration. Noggin expression peaked in young adults (18–45 years), decreased in middle age (46–69 years), and rose again in the elderly (over 69 years), but remained lower than in the youth. Furthermore, upper lumbar discs (T12/L1-L2/3) showed higher Noggin levels than lower lumbar discs (L3/4/-L5/S1). This research elucidates valuable insights into the variability of Noggin concerning sex, age, degree of disc degeneration, and disc segment. It provides implications for IVD health and LBP treatment.

关键词： Low back pain Human intervertebral disc Nucleus pulposus cells Noggin

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Sterility and Endotoxin of Thai Silk Fibroin Solution in a Production Conformed to ISO13485 Standard

Sterility and Endotoxin of Thai Silk Fibroin Solution in a P...

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biomedical engineering International Conference (BMEiCON)

作者： Aksarapak Rattana Rungnapha Yamdech Siriporn Damrongsakkul Juthamas Rattanavaraporn Peerapat Thongnuek Sorada Kanokpanont Antonella Mota Phatcharaphon Nopprang Siripakorn Sangkitporn Acharaporn Dambua Patcharaporn Boonchu Supaporn Suparak Panadda Dhepakson Jutarat Jamkratoke Center of Excellence in Biomaterial Engineering in Medical and Health Department of Chemical Engineering Chulalongkorn University Bangkok Thailand Biomedical Engineering Program Faculty of Engineering Chulalongkorn University Bangkok Thailand BIOtech Research Center Department of Industrial Engineering University of Trento Mattarello TN Italy Department of Medical Sciences Advanced Therapy Medicinal Product Center Ministry of Public Health Nontaburi Thailand The Queen Sirikit Sericulture Center Nakhon Ratchasima Nakorn Ratchasima Thailand

This is the first report of sterile Thai silk fibroin (SF) solution (2-4% (w/v) in water) production at semi-commercial scale under the medical device management system conforming to standard ISO13485:2016. Thai silk cocoons (Bombyx mori, local strain, named Nangnoi Srisaket 1) was maintained, cultured and produced in controlled conditions at The Queen Sirikit Sericulture Center Nakhon Ratchasima, Thailand. The GMP (Medical Device) conformed protocol was optimized. The degummed silk fibers were dissolved in concentrated ionic liquid and purified in the clean room facility of the Department of Medical Sciences, Ministry of Public Health, Thailand. With minimal sterilization of the end product (4%w/w SF Solution), sterility and endotoxin test of the product indicated that the purified proteins were negative for bacteria, mycobacteria, yeast and fungi. The endotoxin levels of all inputs, intermediates and the product was <0.1 EU/ml conforming to the USP standard (for water for injection at the endotoxin level limits <0.25 EU/ml). This indicated that the Thai SF solution can be used for medical and health applications.

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PN-ImTLSM facilitates high-throughput low background single-molecule localization microscopy deep in the cell

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Biophysics Reports 2021年第4期7卷 313-325页

作者： Boxin Xue Caiwei Zhou Yizhi Qin Yongzheng Li Yuao Sun Lei Chang Shipeng Shao Yongliang Li Mengling Zhang Chaoying Sun Renxi He Qian Peter Su Yujie Sun State Key Laboratory of Membrane Biology Biomedical Pioneer Innovation Center (BIOPIC)School of Life SciencesPeking University College of Chemistry and Molecular Engineering Peking University Academy for Advanced Interdisciplinary Studies Peking University Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Beijing Institute of Heart Lung and Blood Vessel Disease Beijing Anzhen HospitalCapital Medical University Department of Geriatric Dentistry Peking University School and Hospital of Stomatology School of Biomedical Engineering Faculty of Engineering and Information TechnologyUniversity of Technology Sydney School of Future Technology Peking University

When imaging the nucleus structure of a cell, the out-of-focus fluorescence acts as background and hinders the detection of weak signals. Light-sheet fluorescence microscopy(LSFM) is a wide-field imaging approach which has the best of both background removal and imaging speed. However, the commonly adopted orthogonal excitation/detection scheme is hard to be applied to single-cell imaging due to steric hindrance. For LSFMs capable of high spatiotemporal single-cell imaging, the complex instrument design and operation largely limit their throughput of data collection. Here, we propose an approach for high-throughput background-free fluorescence imaging of single cells facilitated by the Immersion Tilted Light Sheet Microscopy(ImTLSM). ImTLSM is based on a light-sheet projected off the optical axis of a water immersion objective. With the illumination objective and the detection objective placed opposingly, ImTLSM can rapidly patrol and optically section multiple individual cells while maintaining single-molecule detection sensitivity and resolution. Further, the simplicity and robustness of ImTLSM in operation and maintenance enables high-throughput image collection to establish background removal datasets for deep learning. Using a deep learning model to train the mapping from epi-illumination images to ImTLSM illumination images, namely PN-ImTLSM, we demonstrated crossmodality fluorescence imaging, transforming the epi-illumination image to approach the background removal performance obtained with ImTLSM. We demonstrated that PN-ImTLSM can be generalized to large-field homogeneous illumination imaging, thereby further improving the imaging throughput. In addition, compared to commonly used background removal methods, PN-ImTLSM showed much better performance for areas where the background intensity changes sharply in space, facilitating high-density single-molecule localization microscopy. In summary, PN-ImTLSM paves the way for background-free fluorescence imaging

关键词： Light-sheet fluorescence microscopy Background-free fluorescence imaging Deep learning Single cell imaging Homogeneous illumination Single-molecule localization microscopy

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Cross-system biological image quality enhancement based on the generative adversarial network as a foundation for establishing a multi-institute microscopy cooperative network

arXiv

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arXiv 2024年

作者： Panek, Dominik Rząca, Carina Szczypior, Maksymilian Sorysz, Joanna Misztal, Krzysztof Baster, Zbigniew Rajfur, Zenon Doctoral School of Exact and Natural Sciences Jagiellonian University ul. Lojasiewicza 11 Kraków30-348 Poland Department of Molecular and Interfacial Biophysics Faculty of Physics Astronomy and Applied Computer Science Jagiellonian University ul. Lojasiewicza 11 Kraków30‑348 Poland Undergraduate Program in Biophysics Faculty of Physics Astronomy and Applied Computer Science Jagiellonian University ul. Lojasiewicza 11 Kraków30‑348 Poland Undergraduate program in Biomedical Engineering Faculty of Electrical Engineering Automatics Computer Science and Biomedical Engineering AGH University of Science and Technology Al. A. Mickiewicza 30 Building B-1 Kraków30-059 Poland Division of Computational Mathematics Faculty of Mathematics and Computer Science Jagiellonian University 6 Lojasiewicza St. Kraków Poland

High-quality fluorescence imaging of biological systems is limited by processes like photobleaching and phototoxicity, and also in many cases, by limited access to the latest generations of microscopes. Moreover, low temporal resolution can lead to a motion blur effect in living systems. Our work presents a deep learning (DL) generative-adversarial approach to the problem of obtaining high-quality (HQ) images based on their low-quality (LQ) equivalents. We propose a generative-adversarial network (GAN) for contrast transfer between two different separate microscopy systems: a confocal microscope (producing HQ images) and a wide-field fluorescence microscope (producing LQ images). Our model proves that such transfer is possible, allowing us to receive HQ-generated images characterized by low mean squared error (MSE) values, high structural similarity index (SSIM), and high peak signal-to-noise ratio (PSNR) values. For our best model in the case of comparing HQ-generated images and HQ-ground truth images, the median values of the metrics are 6·10-4, 0.9413, and 31.87, for MSE, SSIM, and PSNR, respectively. In contrast, in the case of comparison between LQ and HQ ground truth median values of the metrics are equal to 0.0071, 0.8304, and 21.48 for MSE, SSIM, and PSNR respectively. Therefore, we observe a significant increase ranging from 14% to 49% for SSIM and PSNR respectively. These results, together with other single-system cross-modality studies, provide proof of concept for further implementation of a cross-system biological image quality enhancement. © 2024, CC BY-SA.

关键词： Fluorescence imaging

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Structural and functional imaging of brains

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Science China Chemistry 2023年第2期66卷 324-366页

作者： Zhichao Liu Ying Zhu Liming Zhang Weiping Jiang Yawei Liu Qiaowei Tang Xiaoqing Cai Jiang Li Lihua Wang Changlu Tao Xianzhen Yin Xiaowei Li Shangguo Hou Dawei Jiang Kai Liu Xin Zhou Hongjie Zhang Maili Liu Chunhai Fan Yang Tian Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai200241China Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai201210China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Chinese Academy of SciencesWuhan National Laboratory for OptoelectronicsWuhan430071China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China Department of Chemistry Tsinghua UniversityBeijing100084China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesInstitute of Translational MedicineShanghai Jiao Tong UniversityShanghai200240China Interdisciplinary Center for Brain Information Brain Cognition and Brain Disease InstituteShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsFaculty of Life and Health SciencesShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Lingang Laboratory Shanghai201602China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai200240China Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen518055China Department of Nuclear Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Analyzing the complex structures and functions of brain is the key issue to understanding the physiological and pathological *** neuronal morphology and local distribution of neurons/blood vessels in the brain have been known,the subcellular structures of cells remain challenging,especially in the live *** addition,the complicated brain functions involve numerous functional molecules,but the concentrations,distributions and interactions of these molecules in the brain are still poorly *** this review,frontier techniques available for multiscale structure imaging from organelles to the whole brain are first overviewed,including magnetic resonance imaging(MRI),computed tomography(CT),positron emission tomography(PET),serial-section electron microscopy(ss EM),light microscopy(LM)and synchrotron-based X-ray microscopy(XRM).Specially,XRM for three-dimensional(3D)imaging of large-scale brain tissue with high resolution and fast imaging speed is ***,the development of elegant methods for acquisition of brain functions from electrical/chemical signals in the brain is *** particular,the new electrophysiology technologies for neural recordings at the single-neuron level and in the brain are also *** also focus on the construction of electrochemical probes based on dual-recognition strategy and surface/interface chemistry for determination of chemical species in the brain with high selectivity and long-term stability,as well as electrochemophysiological microarray for simultaneously recording of electrochemical and electrophysiological signals in the ***,the recent development of brain MRI probes with high contrast-to-noise ratio(CNR)and sensitivity based on hyperpolarized techniques and multi-nuclear chemistry is ***,multiple optical probes and instruments,especially the optophysiological Raman probes and fiber Raman photometry,for imaging and biosensing in live brain are emphas

关键词： brain structure brain function brain chemistry chemical signal biosensing and bioimaging

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