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SSRN 2023年

作者： Jacobson, Kathryn R. Saleh, Aya M. Lipp, Sarah N. Tian, Chengzhe Watson, Audrey R. Luetkemeyer, Callan M. Ocken, Alexander R. Spencer, Sabrina L. Kinzer-Ursem, Tamara L. Calve, Sarah Purdue University Interdisciplinary Life Science Program Purdue University West LafayetteIN47907 United States Weldon School of Biomedical Engineering Purdue University West LafayetteIN47907 United States The Indiana University School of Medicine IndianapolisIN46202 United States Department of Biochemistry University of Colorado Boulder BoulderCO80303 United States BioFrontiers Institute University of Colorado Boulder BoulderCO80303 United States Austrian Academy of Sciences Vienna Austria Paul M. Rady Department of Mechanical Engineering University of Colorado Boulder BoulderCO80309 United States Paul M. Rady Department of Mechanical Engineering United States

The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5-14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome were assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system, which did not directly correlate with single-cell transcriptomic expression profiles, indicating the importance for investigating the proteome in addition to the transcriptome. This resource will guide future research investigating the role of the matrisome during complex tissue development. © 2023, The Authors. All rights reserved.

关键词： Mass spectrometry

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Generation of circularly polarized THz dual pulses

Generation of circularly polarized THz dual pulses

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Conference on Lasers and Electro-Optics (CLEO)

作者： Hao-Keng Wei Hironori Ito Wei-Hong Huang Zai-Wen Chen Ying-Kuan Ko Chien-Ming Tu Atsushi Yabushita Kazuhiko Misawa Chih-Wei Luo Department of Electrophysics National Yang Ming Chiao Tung University Hsinchu Taiwan Graduate Faculty of Interdisciplinary Research Faculty of Engineering University of Yamanashi Yamanashi Japan Department of Applied Physics Tokyo University of Agriculture and Technology Tokyo Japan Department of Biomedical Engineering Tokyo University of Agriculture and Technology Tokyo Japan National Science and Technology Council Taiwan Consortium of Emergent Crystalline Materials (TCECM) Taiwan Institute of Physics and Center for Emergent Functional Matter Science National Yang Ming Chiao Tung University Hsinchu Taiwan National Synchrotron Radiation Research Center Hsinchu Taiwan Department of Physics University of Washington Seattle Washington USA

Circularly polarized THz dual pulses were generated by polarization-twisting optical dual pulses via a modified Michelson interferometer with the turnability of frequency, helicity, and time interval between two pulses.

ISBN: (纸本)9781957171258

关键词： Circular polarization Michelson interferometers Parabolic mirrors Pulse generation Spatial light modulators Wire grid polarizers

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Cultivation of Chlorella sp. HS2 using wastewater from soy sauce factory

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Chemosphere 2023年 342卷 140162页

作者： Kim, Minsik Lee, Sang-Ah Yun, Jin-Ho Ko, So-Ra Cho, Dae-Hyun Kim, Hee Su Oh, Hee-Mock Kim, Hee-Sik Ahn, Chi-Yong Daejeon34141 Korea Republic of Dept. of Biological Engineering Inha University Incheon22212 Korea Republic of Faculty of Biotechnology College of Applied Life Sciences Jeju National University Jeju63243 Korea Republic of Interdisciplinary Graduate Program in Advance Convergence Technology and Science Jeju National University Jeju63243 Korea Republic of Department of Integrative Biotechnology Sungkyunkwan University Gyeonggi-do Suwon16419 Korea Republic of FarmHannong Chungcheongnam-do Nonsan33010 Korea Republic of

Incorporation of wastewater from industrial sectors into the design of microalgal biorefineries has significant potential for advancing the practical application of this emerging industry. This study tested various food industrial wastewaters to assess their suitability for microalgal cultivation. Among these wastewaters, defective soy sauce (DSS) and soy sauce wastewater (SWW) were chosen but DSS exhibited the highest nutrient content with 13,500 ppm total nitrogen and 3051 ppm total phosphorus. After diluting DSS by a factor of 50, small-scale cultivation of microalgae was conducted to optimize culture conditions. SWW exhibited optimal growth at 25–30 °C and 300–500 μE m−2 s−1, while DSS showed optimal growth at 30–35 °C. Based on a 100-mL lab-scale and 3-L outdoor cultivation with an extended cultivation period, DSS outperformed SWW, exhibiting higher final biomass productivity. Additionally, nutrient-concentrated nature of DSS is advantageous for transportation at an industrial scale, leading us to select it as the most promising feedstock for microalgal cultivation. With further optimization, DSS has the potential to serve as an effective microalgal cultivation feedstock for large-scale biomass production. © 2023 The Authors

关键词： Microalgae

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A deep learning for automated segmentation of skeletal muscle at the third lumbar vertebra in computed tomography images

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Journal of Medical Imaging and Radiation Sciences 2022年第4期53卷 S37-S37页

作者： Cheewakul, Jongwat Yanaihap, N Yue-roh, T Kepan, N Thiangsook, W Rojanaaumpawan, Th Ina, N Radiology Department Faculty of Medicine Prince of Songkhla University Songkhla Thailand Biomedical Engineering Program Department of Electrical Engineering Faculty of Engineering Prince of Songkhla University Songkhla Thailand

IntroductionThe purpose of this study is to apply deep learning to segment and visualize the skeletal muscle in the middle of third lumbar vertebra on computed tomography images. MethodsBetween July 1 and December 31, 2021, patients aged 20 to 60 who had computed tomography at Songklanagarind Hospital computed tomography center will have their images of the abdomen axial slice at the middle of the third lumbar vertebra gathered. We use 215 images, including computed tomography (original images) and binary images, for data preparation using region growing and manual segmentation in MATLAB by over ten years experienced radiation technologist. Additionally, the dataset is separated into three distinct categories: train, validation, and verification. The deep learning model was developed using python and a 2D dilated residual attention U-Net architecture. And Thresholding-based solutions were desired to prevent over detection of the model's exterior muscle area. ResultOn the validation set, the model's accuracy, Binary Cross-Entropy loss, and similarity Intersection over union (IoU)were 0.9926, 0.0387, and 0.8869, respectively, for the model performance of automated segmentation in the middle of the third lumbar vertebra using computed tomography images. In addition, the rate of validation errors decreases from 0.99 percent to 0.49 percent. ConclusionFinally, when employing deep learning for automated segmentation and visualization of skeletal muscle in the middle of the third lumbar vertebra on computed tomography images was highly effective.

关键词： Deep learning Convolution Neural Network 2D U-net Computed Tomography Image segmentation

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Sustainable valorization of grass biomass via hydrothermal pretreatment for biogas and biofuel co-production

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Journal of Environmental Management 2025年 389卷

作者： Arada Hmeekong Laemthong Chuenchom Boonya Charnnok Sumate Chaiprapat Department of Civil and Environmental Engineering Faculty of Engineering Prince of Songkla University Hat Yai Songkhla 90110 Thailand Department of Chemistry Faculty of Science Prince of Songkla University Hat Yai Songkhla 90110 Thailand Energy Technology Program Department of Interdisciplinary Engineering Faculty of Engineering Prince of Songkla University Hat Yai Songkhla 90110 Thailand PSU Energy Systems Research Institute (PERIN) Prince of Songkla University Hat Yai Songkhla 90110 Thailand

This study investigates the potential of hydrothermal pretreatment to enhance the energy recovery and waste management of Napier grass ( Pennisetum purpureum ) as a co-substrate in pig farming operations. Pig farms in Thailand generate wastewater rich in organic matter and nutrients, and efficient treatment is essential for meeting environmental discharge standards and reducing greenhouse gas emissions. Two pretreatment strategies were evaluated: a low-temperature hydrothermal fermentative pretreatment (HFP) and a high-temperature thermal hydrolysis pretreatment (THP). Napier grass, characterized by its high lignocellulosic content and rapid growth, was processed at various temperatures (35–100 °C for HFP; 125–200 °C for THP) and reaction times (6–72 h) to produce distinct liquid and solid fractions. Biochemical methane potential (BMP) assays demonstrated that HFP at 35 °C for 12 h yielded the highest overall methane production (184.5 m 3 CH 4 per ton of dry biomass), reflecting a 65 % increase in biodegradability relative to untreated grass. Regression analysis further elucidated the interactive effects of temperature and reaction time on methane yields, while mass balance evaluations compared alternative pathways for the valorization of the HFP solids. Notably, converting the solids into biomass pellets delivered a superior net energy output compared to further anaerobic digestion. Overall, the integrated approach of using hydrothermal pretreatment, especially via HFP, not only improves biogas yields but also aligns with sustainable agricultural practices by reducing waste, mitigating environmental impacts, and enhancing the energy efficiency of pig farming systems.

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#52. Computational modeling of signaling pathways and cell-cell interactions driving tumor-induced bone disease

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Journal of Bone Oncology 2024年 45卷 100590-100590页

作者： Vega, Alexandra Gutierrez Alshafeay, Saja Bennett, Natalie Beadle, Erik Rhoades, Julie Harris, Leonard A. Interdisciplinary Graduate Program in Cell and Molecular Biology University of Arkansas United States Vanderbilt Center for Bone Biology Vanderbilt University School of Medicine United States Department of Biomedical Engineering University of Arkansas United States

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Design of Electrical Impedance Tomography (EIT) for Medical Imaging Application 6

Design of Electrical Impedance Tomography (EIT) for Medical ...

引用

6th IEEE International Conference on Smart Instrumentation, Measurement and Application, ICSIMA 2019

作者： Wicaksono, Arbariyanto Mahmud Basari Faculty of Engineering Universitas Indonesia Biomedical Engineering Course Program Department of Electrical Engineering Indonesia Research Center for Biomedical Engineering Universitas Indonesia Kampus UI Depok West-Java16424 Indonesia

ISBN: (纸本)9781728139524

The rise of imaging in medical field has boost the progress of new imaging method and modality that safer, cheaper, and faster. Electrical Impedance Tomography (EIT) is one of imaging modalities that provides safe medical imaging through non ionizing method. The downside of the EIT method is the complex algorithm needed to produce imaging result and low resolution. This study is aimed to provide a simple EIT imaging system that are easily used and developed. The proposed system designed in this study is a MatlabTM and Arduino based imaging system, which are then tested under several observation object scenario. The imaging results that are produced by the system are able to represent the varying observational objects structure. Impedance measurement method that is implemented in the proposed system is a two-point technique using 8 electrodes and 16 electrodes with a frequency of 50 kHz. The main observation object of this system is an acrylic cup filled with tap water that has a TDS (Total Dissolved Solids) of 300 ppm. The proposed EIT imaging system is considered as a simple construction that is able to recognize two different objects according to its different impedance values. © 2019 IEEE.

关键词： Imaging systems

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RELATIVE SIMPLICITY OF THE UNIVERSAL COVERINGS OF TRANSFORMATION GROUPS AND TSUBOI’S METRIC

arXiv

引用

arXiv 2024年

作者： Kawasaki, Morimichi Kimura, Mitsuaki Kodama, Hiroki Matsuda, Yoshifumi Matsushita, Takahiro Orita, Ryuma Department of Mathematics Faculty of Science Hokkaido University North 10 West 8 Kita-ku Hokkaido Sapporo060-0810 Japan Department of Mathematics Osaka Dental University 8-1 Kuzuha-hanazono-cho Osaka Hirakata573-1121 Japan Center for Mathematical Engineering Musashino University 3-3-3 Ariake Koto-ku Tokyo135-8181 Japan Interdisciplinary Theoretical and Mathematical Sciences Program RIKEN 2-1 Hirosawa Saitama Wako-shi351-0198 Japan Department of Mathematical Sciences College of Science and Engineering Aoyama Gakuin University 5-10-1 Fuchinobe Chuo-ku Kanagawa Sagamihara252-5258 Japan Department of Mathematical Sciences Faculty of Science Shinshu University Nagano390-8621 Japan Department of Mathematics Faculty of Science Niigata University Niigata950-2181 Japan

Many transformation groups on manifolds are simple, but their universal coverings are not. In the present paper, we study the concept of relatively simple group, that is, a group with the maximum proper normal subgroup. We show that many examples of universal coverings of transformation groups are relatively simple, including the universal covering (formula presented) of the group of Hamiltonian diffeomorphisms of a closed symplectic manifold (M, ω). Tsuboi constructed a metric space M(G) for a simple group G. We generalize his construction to relatively simple groups, and study their large scale geometric structure. In particular, Tsuboi’s metric space of (formula presented) is not quasi-isometric to the half line for every closed symplectic manifold (M, ω).MSC Codes 20A05, 20E32, 51F30, 53D22, 53D40, 57S05 Copyright © 2024, The Authors. All rights reserved.

关键词： Hamiltonians

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Profile of bovine teeth potency as hydroxyapatite scaffold

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AIP Conference Proceedings 2023年第1期2536卷

作者： Endanus Harijanto Anita Yuliati Prihartini Widiyanti Dental Material Department Faculty of Dentistry Universitas Airlangga Surabaya Indonesia Biomedical Engineering Study Program Department of Physics Faculty of Science and Technology Universitas Airlangga Surabaya Indonesia

Background: Bone destructions in the oral cavity are due to trauma, chronic infection, congenital malformations, or resection surgery, most often by using an autologous bone graft technique. The problem using this technique are limited donors and suppliers, high cost, host potential imune response becauce accepts foreign bone tissue also potential transmission diseases. Another solution in tissue engineering techniques is using the scaffold. Hidroxyapatite is one of the osteoconductive potentialy ceramic materials in scaffold fabrication. Objective: To prove that bovine teeth hydroxyapatite have characteristic as scaffold for bone tissue regeneration. Methods: HA was obtained by calcining bovine teeth at 1000°C for 3 hours, Fourier Transform Infra Red Spectroscopy (FTIR) test , Energy Dispersive X-Ray (EDX) test and X-Ray Diffraction (XRD) test. Results: Bovine teeth hydroxyapatite to showed of hydroxyl, phosphate and carbonate functional groups (FTIR test). Average Ca/P=1.97. Conclusion: Hydroxyapatite from bovine teeth has potency as scaffold through the chemistry and physical characteristics.

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Lower limb joint angular phase plane coordination analysis on standard maximum vertical jump

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Gait & Posture 2021年 90卷 205-206页

作者： Rodrigues, C. Correia, M. Abrantes, J. Rodrigues, B. Nadal, J. INESC TEC - Institute for Systems and Computer Engineering- Technology and Science C-BER - Centre for Biomedical Engineering Research Porto Portugal University of Porto Faculty of Engineering Porto Portugal Lusófona University CICANT Centre for Research in Applied Communication Culture and New Technologies- MovLab Laboratory of Technologies for Interactions and Interfaces Lisbon Portugal Federal University of Pernambuco Department of Electronic and Systems Recife Brazil Federal University of Rio de Janeiro Biomedical Engineering Program Rio de Janeiro Brazil

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