检索结果-内蒙古大学图书馆

Protocol to characterize longitudinal gut motility in mice using transit time, tissue harvest, and whole-mount immunostaining

引用

STAR Protocols 2025年第2期6卷 103761页

作者： Frith, Mary E. Kashyap, Purna C. Linden, David R. Chang, Eugene B. Interdisciplinary Scientist Training Program University of Chicago Chicago 60637 IL United States Department of Medicine University of Chicago Chicago 60637 IL United States Division of Gastroenterology and Hepatology Department of Medicine Mayo Clinic Rochester 55905 MN United States Enteric Neuroscience Program Department of Physiology and Biomedical Engineering Mayo Clinic Rochester 55905 MN United States

Transit time is a key in vivo metric of gastrointestinal (GI) motility, which is a physiologic readout of cellular communication within the enteric system. Here, we present a protocol to characterize longitudinal gut motility in mice. We describe steps for transit testing, whole-mount immunostaining, and tissue harvest. We then detail procedures for image processing and manual cell counting. This protocol seeks to minimize inter-trial variability while assessing cellular and molecular features that may underpin motility differences between experimental conditions. For complete details on the use and execution of this protocol, please refer to Frith et al.¹ © 2025 The Authors

关键词： Metabolism Model Organisms Systems biology

来源：评论

学校读者我要写书评

暂无评论

The Bacterial Outer Membrane Vesicle-Cloaked Immunostimulatory Nanoplatform Reinvigorates T Cell Function and Reprograms Tumor Immunity

引用

ACS Nano 2025年 2025 May 20页

作者： Lin, Yu-Han Chen, Chia-Wei Chen, Mei-Yi Xu, Li Tian, Xuejiao Cheung, Siu-Hung Wu, Yen-Ling Siriwon, Natnaree Wu, Si-Han Mou, Kurt Yun Taiwan International Graduate Program in Molecular Medicine National Yang Ming Chiao Tung University and Academia Sinica Taipei 11529 Taiwan Graduate Institute of Nanomedicine and Medical Engineering Taipei Medical University Taipei 11031 Taiwan Institute of Biomedical Sciences Academia Sinica Taipei 11529 Taiwan Research Center for Applied Sciences Academia Sinica Taipei 11529 Taiwan Chakri Naruebodindra Medical Institute Faculty of Medicine Ramathibodi Hospital Mahidol University Samutprakarn 10540 Thailand International Ph.D. Program in Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan Nano Science and Technology Program Taiwan International Graduate Program Academia Sinica Taipei 11529 Taiwan Department of Engineering and System Science National Tsing Hua University Hsinchu 30013 Taiwan

Bacterial outer membrane vesicles (OMVs) represent powerful immunoadjuvant nanocarriers with the capacity to reprogram the tumor microenvironment (TME) and activate immune responses. Here, we investigate a nanotherapeutic platform integrating immunostimulatory cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODNs, hereafter termed CpG) into mesoporous silica nanoparticles cloaked with OMVs (CpG@MSN-PEG/PEI@OMVs) for cancer immunotherapy. Systemic administration of these nanohybrids facilitates precise tumor targeting, induces antitumor cytokines such as IFNγ, and suppresses immunosuppressive cytokine TGF-β, reshaping the TME. Additionally, CpG@MSN-PEG/PEI@OMVs promote M1 macrophage polarization, dendritic cell maturation, and the generation of durable tumor-specific immune memory, resulting in pronounced tumor regression with minimal systemic toxicity. The platform demonstrates efficacy against metastatic and solid tumor models including 4T1 breast and MC38 colorectal cancers. Transcriptomic analyses reveal that CpG@MSN-PEG/PEI@OMVs enhance mitochondrial oxidative phosphorylation in T cells within tumor-draining lymph nodes, mitigating T cell exhaustion and restoring metabolic fitness. These results support the potential of CpG@MSN-PEG/PEI@OMVs as a modular nanoplatform to modulate innate and adaptive immunity in cancer immunotherapy. © 2025 The Authors. Published by American Chemical Society.

关键词： bacterial outer membrane vesicles mesoporous silica nanoparticles mitochondrial activity T cell exhaustion phenotypes tumor microenvironment

来源：评论

学校读者我要写书评

暂无评论

Amplifying X-ray-Induced Charge Transfer Facilitates Direct Sensitization of Photosensitizers in Radiotherapy

引用

ACS Nano 2025年第17期19卷 16775-16793页

作者： Zhang, Da Chen, Qingjing Zhang, Junrong Xing, Xiaohua Zhou, Yang Ou, Xiangyu Dai, Shuheng Chen, Qiushui Liu, Xiaolong Chen, Xiaoyuan Zeng, Yongyi The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University Fuzhou350025 China Departments of Diagnostic Radiology Surgery Chemical and Biomolecular Engineering and Biomedical Engineering Yong Loo Lin School of Medicine Faculty of Engineering National University of Singapore Singapore119074 Singapore Clinical Imaging Research Centre Centre for Translational Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore138667 Singapore Nanomedicine Translational Research Program NUS Center for Nanomedicine Yong Loo Lin School of Medicine National University of Singapore Singapore138667 Singapore CAS Key Laboratory of Design and Assembly of Functional Nanostructures Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou350002 China Linköping University LinköpingSE-58183 Sweden MOE Key Laboratory for Analytical Science of Food Safety and Biology Fuzhou University Fuzhou350108 China Department of General Surgery Fujian Medical University Union Hospital Fuzhou350001 China Mengchao Med-X Center Fuzhou University Fuzhou350116 China Liver Disease Center The First Affiliated Hospital of Fujian Medical University Fuzhou350005 China

X-ray-induced photodynamic therapy offers substantial promise for treating deep-seated tumors, but it is still limited by highly inefficient energy transfer processes and the stringent requirements for scintillators with high luminescence quantum yield and significant singlet-triplet intersystem crossing ratios. Herein, we describe X-ray-induced electron-dynamic therapy (X-eDT), which obviates the need for intersystem crossing by exposing nonluminescent hafnium-silica nanoparticles to X-rays, to generate high-energy electrons that can sensitize lower-lying triplet states of various photosensitizers. Our approach strongly induced the production of singlet oxygen (6.18-fold) in vitro even at lower X-ray doses, and in mice it strongly inhibited the growth of xenografts derived from liver, breast, or colon cancer cell lines (CDX), and growth of patient-derived xenografts (PDX) of hepatocellular carcinoma. In these CDX preclinical systems, X-eDT was not only effective against the irradiated xenograft but also against untreated xenografts in the same animal, and these abscopal effects involved enhanced tumor infiltration by CD4+T cells, CD8+T cells, and IFN-γ-polarized M1 macrophages within the tumor microenvironment. X-eDT even stimulated the production of memory T cells that inhibited rechallenges after treatment. These findings suggest that X-eDT can be effective against primary and metastatic tumors as well as tumor recurrence, which makes it much more powerful than conventional X-PDT. © 2025 American Chemical Society.

关键词： Animal cell culture

来源：评论

学校读者我要写书评

暂无评论

A BLOOD-VESSEL MODEL-BASED ON VELOCITY PROFILES

引用

COMPUTERS IN BIOLOGY AND MEDICINE 1993年第4期23卷 295-300页

作者： BARNEA, O Biomedical Engineering Program Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel

A simple model of pressure-flow relationship in blood vessels was developed. The calculation of the model components was based on velocity profiles in the vessels. The flat velocity profile in the entrance of the vessel was considered. By using mean pressure over the cross-section and assuming a polynomial approximation of the velocity profile, it is shown that the resistance of a vessel segment increases with increased flatness of the velocity profile. Moreover, the analysis provides a means to calculate the resistance of a vessel segment based on the shape of the velocity profiles in that segment. The analysis shows that the inertance element of the segment is not affected by the shape of the velocity profile.

关键词： CARDIOVASCULAR MODEL COMPUTER MODEL BLOOD FLOW ARTERIAL RESISTANCE

来源：评论

学校读者我要写书评

暂无评论

Evaluation of hemiplegic gait using accelerometer

引用

Transactions of the Korean Institute of Electrical Engineers 2017年第11期66卷 1634-1640页

作者： Lee, Jun Seok Park, Sooji Shin, Hangsik Interdisciplinary Program of Biomedical Engineering Chonnam National University Gwangju Korea Republic of Department of Biomedical Engineering Chonnam National University Yeosu Korea Republic of

The study aims to distinguish hemiplegic gait and normal gait using simple wearable device and classification algorithm. Thus, we developed a wearable system equipped three axis accelerometer and three axis gyroscope. The developed wearable system was verified by clinical experiment. In experiment, twenty one normal subjects and twenty one patients undergoing stroke treatment were participated. Based on the measured inertial signal, a random forest algorithm was used to classify hemiplegic gait. Four-fold cross validation was applied to ensure the reliability of the results. To select optimal attributes, we applied the forward search algorithm with 10 times of repetition, then selected five most frequently attributes were chosen as a final attribute. The results of this study showed that 95.2% of accuracy in hemiplegic gait and normal gait classification and 77.4% of accuracy in hemiplegic-side and normal gait classification. © 2017 The Korean Institute of Electrical Engineers.

关键词： Patient treatment

来源：评论

学校读者我要写书评

暂无评论

Wearable continuous diffusion-based skin gas analysis

引用

Nature Communications 2025年第1期16卷 1-15页

作者： Clausen, David Farley, Max Little, Abigail Kasper, Kevin Moreno, Joseph Limesand, Larissa Gutruf, Philipp Department of Biomedical Engineering University of Arizona Tucson 85721 AZ United States Department of Physiology University of Arizona Tucson 85721 AZ United States Department of Electrical and Computer Engineering University of Arizona Tucson 85721 AZ United States Bio5 Institute University of Arizona Tucson 85721 AZ United States Neuroscience Graduate Interdisciplinary Program University of Arizona Tucson 85721 AZ United States

Biophysical signals such as motion and optically acquired hemodynamics represent foundational sensing modalities for wearables. Expansion of this toolset is vital for the progression of digital medicine. Current efforts utilize biofluids such as sweat and interstitial fluid with primarily adhesively mounted sensors that are fundamentally limited by epidermal turnover. A class of potential biomarkers that is largely unexplored are gaseous emissions from the body. In this work, we introduce an approach to capture emission of gas from the skin with a leaky cavity designed to allow for diffusion-based ambient gas exchange with the environment. This approach, coupled with differential measurement of ambient and in-cavity gas concentrations, allows for the real-time analysis of sweat rate, VOCs, and CO₂ while performing everyday tasks. The resulting biosignals are recorded with temporal resolutions that exceed current methodology, providing unparalleled insight into physiological processes without requiring sensor replacement over weeks at a time. © The Author(s) 2025.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Asymmetric Modulation of Brain Connectivity by Anodal Transcranial Direct Current Stimulation in Healthy Individuals: A Single-Blind, Randomized Sham-Controlled Trial

引用

Human Brain Mapping 2025年第7期46卷 e70218页

作者： Carther-Krone, Tiffany McAllister, Zachary A. Choi, Eun Hyung Ryner, Lawrence Ko, Ji Hyun Department of Human Anatomy and Cell Science Max Rady College of Medicine University of Manitoba Winnipeg Canada PrairieNeuro Research Centre Kleysen Institute for Advanced Medicine Winnipeg Health Science Centre Winnipeg Canada Department of Radiology Max Rady College of Medicine University of Manitoba Winnipeg Canada Graduate Program in Biomedical Engineering Price Faculty of Engineering University of Manitoba Winnipeg Canada

Transcranial direct current stimulation (tDCS) applied to the dorsolateral prefrontal cortex (DLPFC) has shown asymmetric behavioral effects, though the underlying neurophysiological mechanisms remain unclear. In this preliminary study with 34 healthy individuals, tDCS was applied to either the left or right DLPFC or a sham group. Behavioral and neurophysiological changes were examined by the Stroop test and resting-state fMRI, respectively, which were measured before and after a 15-min tDCS session. Seed-to-voxel connectivity analysis with seeds placed under the tDCS target regions (F3 and F4) showed no significant changes, but voxel-to-voxel whole-brain intrinsic connectivity (IC) analysis revealed significant 3 × 2 interaction effects (stimulation site × time) in the right DLPFC (18 mm off from the F4). Post hoc analysis showed that only the right DLPFC stimulation led to an increase in IC from pre- to post-stimulation. Consistent with this finding, right DLPFC stimulation improved Stroop task performance measured by increased interference score, which represents better inhibition of irrelevant information. These findings provide further insights into the hemispheric difference of tDCS effects and its underlying neurophysiological mechanisms. However, the small sample size limits the generalizability of the results and necessitates further research with a larger cohort for confirmation. © 2025 The Author(s). Human Brain Mapping published by Wiley Periodicals LLC.

关键词： dorsolateral prefrontal cortex fMRI interference score intrinsic connectivity Stroop task transcranial direct current stimulation

来源：评论

学校读者我要写书评

暂无评论

PC-based tele-audiometry

引用

TELEMEDICINE JOURNAL AND E-HEALTH 2007年第5期13卷 501-508页

作者： Choi, Jong Min Lee, Haet Bit Park, Cheol Soo Oh, Seung Ha Park, Kwang Suk Interdisciplinary Program for Biomedical Engineering College of Medicine Seoul National University Seoul Republic of Korea. Telemedicine Association

A personal computer (PC)-based audiometer was developed for interactive remote audiometry. This paper describes a tele-audiometric system and evaluates the performance of the device when compared with conventional face-to-face audiometry. The tele-audiometric system is fully PC-based. A sound card featuring a high-quality digital-to-analog converter is used as a pure- tone generator. The audiometric programs were developed based on Microsoft Windows in order to maximize usability. Audiologists and their subjects can use the tele- audiometry system as one would utilize any PC application. A calibration procedure has been applied for the standardization of sound levels in the remote system. The performance of this system was evaluated by comparing PC-based audiometry with the conventional clinical audiometry system for 37 subjects. Also, performance of the PC-based system was evaluated during use at a remote site. The PC-based audiometry system estimated the audiometric threshold with an error of less than 2.3 dBSPL. Only 10.7% of the results exhibited an error greater than 5 dBSPL during use at a remote site. The PC-based tele-audiomerty showed acceptable results for use at a remote site. This PC- based system can be used effectively and easily in many locations that have Internet access but no local audiologists.

关键词： AUDIOMETRY TELECOMMUNICATION in medicine MEDICAL informatics MEDICAL care -- Computer network resources

来源：评论

学校读者我要写书评

暂无评论

A COMPUTER-MODEL FOR ANALYSIS OF FLUID RESUSCITATION

引用

COMPUTERS IN BIOLOGY AND MEDICINE 1993年第6期23卷 443-454页

作者： BARNEA, O SHEFFER, N Biomedical Engineering Program Fleishmann Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel

Injuries involving massive blood loss, such as burns, combat wounds, and injuries resulting from car accidents, require fluid resuscitation. The risk involved in fluid therapy is overloading of the circulation, resulting in pulmonary edema which can lead to death. The risk of pulmonary edema may be eliminated by proper determination of maximal infusion volume and rate. Reabsorption of fluid from the extravascular compartment and infusion of fluid following blood loss results in reduction of the hematocrit. This is accompanied by an increase in the heart's preload and afterload. Coronary driving pressure and flow increase due to increased volume. However, because of the reduced hematocrit this increase in coronary flow may not be sufficient to compensate the myocardium, in terms of oxygen supply, for the increase in oxygen consumption. A model of the cardiovascular system, including an extravascular compartment, was designed to analyze the effects of fluid infusion on hemodynamic variables, cardiac oxygen balance, and the redistribution of fluid between intravascular and extravascular compartments. The results indicate that edema is not the only possible adverse effect of overloading the cardiovascular system with fluids. The simulation demonstrated that in certain cases the heart's oxygen balance can become negative. Limiting the rate of infusion can reduce this risk.

关键词： RESUSCITATION COMPUTER SIMULATION MODELS CARDIOVASCULAR

来源：评论

学校读者我要写书评

暂无评论

Mitochondrial activation via calcium peroxide/carrageenan/soybean lecithin-derived polypyrrole phototherapeutic microparticles

引用

Colloids and Surfaces B: Biointerfaces 2025年 254卷 114787页

作者： Chuang, Sih-Chi Yu, Shih-An Hung, Pei-Chia Chuang, Andrew E.-Y. Liang, Jia-Wei Sun, Chia-Lu Rethi, Lekshmi Fang, Hsu-Wei Nguyen, Hieu Trung Tsai, Yao-Tung Weng, Pei-Wei Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei10608 Taiwan Doctoral Program of Biochemical and Biomedical Engineering National Taipei University of Technology Taipei10608 Taiwan Taipei Medical University Derived Company-Hsuan Chen Technology CO. LTD. New Taipei City22055 Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University New Taipei City Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University New Taipei City Taiwan Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei11696 Taiwan WELLCARE Health Care Supply Co. Ltd. Taipei Taiwan Department of Orthopedics and Trauma Faculty of Medicine University of Medicine and Pharmacy at Ho Chi Minh city Ho Chi Minh City700000 Viet Nam Department of Orthopaedic Surgery Tri-Service General Hospital National Defense Medical Center No. 325 Sec. 2 Chenggong Rd. Neihu Dist. Taipei City11490 Taiwan Division of Traumatology Department of Surgery Tri-Service General Hospital National Defense Medical Center No. 325 Sec. 2 Chenggong Rd. Neihu Dist. Taipei City11490 Taiwan Department of Orthopedics School of Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan Department of Orthopedics Shuang Ho Hospital Taipei Medical University New Taipei City Taiwan

Mitochondria, often referred to as the "powerhouses" of eukaryotic cells, play a critical role in maintaining cellular homeostasis and supporting essential life functions, particularly in cell survival and apoptosis. This study introduces a composite formulation—combining carrageenan (Car), calcium peroxide (CaO2), and microparticles, derived from soybean oil lecithin, enhanced with photothermally active polypyrrole (PPy)—to create Car-Lec-PPy MP, aimed at promoting mitochondrial activation in cells. The investigation focuses on intercellular mitochondrial transfer and activation as a therapeutic strategy. The formulation seeks to stimulate mitochondrial biogenesis and enhance cellular resilience through targeted mitochondrial activation, leveraging the synergistic effects of near-infrared (NIR) therapy. In the in vivo experiment, phototherapeutic Car-Lec-PPy MP was administered to rodent muscle tissue. The results showed increased expression of heat-shock proteins and reduced oxidative stress in the treated muscle. Preliminary findings suggest that this approach not only boosts mitochondrial activity but also improves cell proliferation and survival, presenting significant potential for applications in regenerative medicine and cellular therapy. © 2025 Elsevier B.V.

关键词： Mitochondria

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：