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A generalized adder for cell size homeostasis: Effects on stochastic clonal proliferation

Biophysical Journal 2025年第9期124卷 1376-1386页

作者： Nieto, César Vargas-García, César Augusto Singh, Abhyudai Department of Electrical and Computer Engineering University of Delaware Newark DE United States AGROSAVIA - Corporacion Colombiana de Investigacion Agropecuaria Cundinamarca Mosquera Colombia Department of Electrical and Computer Engineering Biomedical Engineering Mathematical Sciences Interdisciplinary Neuroscience Program University of Delaware Newark DE United States

Measurements of cell size dynamics have revealed phenomenological principles by which individual cells control their size across diverse organisms. One of the emerging paradigms of cell size homeostasis is the adder, where the cell cycle duration is established such that the cell size increase from birth to division is independent of the newborn cell size. We provide a mechanistic formulation of the adder, considering that cell size follows any arbitrary nonexponential growth law. Our results show that the main requirement to obtain an adder regardless of the growth law (the time derivative of cell size) is that cell cycle regulators are produced at a rate proportional to the growth law, and cell division is triggered when these molecules reach a prescribed threshold level. Among the implications of this generalized adder, we investigate fluctuations in the proliferation of single-cell-derived colonies. Considering exponential cell size growth, random fluctuations in clonal size show a transient increase and then eventually decay to zero over time (i.e., clonal populations become asymptotically more similar). In contrast, several forms of nonexponential cell size dynamics (with adder-based cell size control) yield qualitatively different results: clonal size fluctuations monotonically increase over time, reaching a nonzero value. These results characterize the interplay between cell size homeostasis at the single-cell level and clonal proliferation at the population level, explaining the broad fluctuations in clonal sizes seen in barcoded human cell lines. © 2025 Biophysical Society

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Harnessing novel engineered feeder cells expressing activating molecules for optimal expansion of NK cells with potent antitumor activity

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Cellular & Molecular Immunology 2022年第2期19卷 296-298页

作者： Bokyung Min Bitna Yang You-Sun Kim Gyeong Min Park HyunAh Kim Hyojin Kim Eun-Ji Kim Yu Kyeong Hwang Eui-Cheol Shin Sungyoo Cho BioMedical Science and Engineering Interdisciplinary Program KAISTDaejeonKorea Cell Therapy Research Center GC LabCellYonginKorea Laboratory of Immunology and Infectious Diseases Graduate School of Medical Science and EngineeringKAISTDaejeonKorea

Natural killer(NK)cells play an important role in the antitumor immune response as the major cytotoxic lymphocytes in the innate immune *** cells without any genetic modifications have been used for both autologous and allogeneic ***,many reports have suggested that allogeneic NK cells can reduce the recurrence of disease and improve survival through a graft-versus-leukemia effect without GVHD[1,2].

关键词： killer expressing optimal

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Comparison of Hemodynamic Circulation in Pulsatile and Nonpulsatile Extracorporeal Membrane Oxygenation Systems Using an In-Vitro Heart Model

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ASAIO Journal 2025年 2025 Apr 17页

作者： Yoo, Changyoung Jang, Hyun-Woo Kang, Seongmin Choi, Seong-Wook Interdisciplinary Program in Biohealth-Machinery Convergence Engineering Kangwon National University Chuncheon-si Korea Republic of Program of Mechanical and Biomedical Engineering College of Engineering Kangwon National University Chuncheon-si Korea Republic of

This study conducts an in-vitro experiment using a mock circulation system that mimics human circulation to assess the veno-arterial extracorporeal membrane oxygenation (ECMO) system. This study aims to compare the hemodynamic effects of centrifugal and pulsatile ECMO systems on the body using an in-vitro cardiogenic shock model. The heart model used in this study involves a contracting blood sac with inlet and outlet valves, capable of maintaining arterial pressure between 80 and 120 mm Hg while delivering a blood flow of 1.8-2 L/min, effectively replicating a cardiogenic shock model. The contraction force in the heart model was generated using a pneumatic cylinder and gradually decreased to simulate reduced cardiac function. The initial blood flow rates of both ECMO systems were maintained at 2 L/min under identical conditions for a fair comparison. Upon reducing the stroke volume of the heart to 35 ml, the ECMO system with counter-pulsation control increased the cardiac output by 10.7% and systemic circulation by 3.8% compared with the conventional ECMO system. This study demonstrates the hemodynamic benefits provided by the sustained counter-pulsation in an in-vitro weakened heart model. Pulsatile flow ECMO systems may serve as an alternative to address the limitations of continuous flow ECMO systems. © ASAIO 2025.

关键词： Heart

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Bioinspired MXene-Based Soft Actuators Exhibiting Angle-Independent Structural Color

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Nano-Micro Letters 2023年第1期15卷 1-13页

作者： Pan Xue Yuanhao Chen Yiyi Xu Cristian Valenzuela Xuan Zhang Hari Krishna Bisoyi Xiao Yang Ling Wang Xinhua Xu Quan Li School of Materials Science and Engineering Tianjin UniversityTianjin 300350People’s Republic of China Institute of Advanced Materials School of Chemistry and Chemical EngineeringTech Key Laboratory for Biomedical ResearchSoutheast Universityand Jiangsu Province HiNanjing 211189People’s Republic of China Advanced Materials and Liquid Crystal Institute and Chemical Physics Interdisciplinary Program Kent State UniversityKentOH 44242USA

In nature,many living organisms exhibiting unique structural coloration and soft-bodied actuation have inspired scientists to develop advanced structural colored soft actuators toward biomimetic soft ***,it is challenging to simultaneously biomimic the angle-independent structural color and shape-morphing capabilities found in the plum-throated cotinga flying ***,we report biomimetic MXene-based soft actuators with angle-independent structural color that are fabricated through controlled self-assembly of colloidal SiO_(2) nanoparticles onto highly aligned MXene films followed by vacuum-assisted infiltration of polyvinylidene fluoride into the *** resulting soft actuators are found to exhibit brilliant,angle-independent structural color,as well as ultrafast actuation and recovery speeds(a maximum curvature of 0.52 mm−1 can be achieved within 1.16 s,and a recovery time of~0.24 s)in response to acetone *** proof-of-concept illustrations,structural colored soft actuators are applied to demonstrate a blue gripper-like bird’s claw that can capture the target,artificial green tendrils that can twine around tree branches,and an artificial multicolored butterfly that can flutter its wings upon cyclic exposure to acetone *** strategy is expected to offer new insights into the development of biomimetic multifunctional soft actuators for somatosensory soft robotics and next-generation intelligent machines.

关键词： Bioinspired soft actuator Angle-independent structural color MXene liquid crystals Soft robotics

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HIFU thermal lesion difference between simulated human phantom and tissue-mimicking phantom 24

HIFU thermal lesion difference between simulated human phant...

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24th International Congress on Acoustics, ICA 2022

作者： Kim, Seong Chan Choi, Min Joo Jeju National University Interdisciplinary Postgraduate Program of Biomedical Engineering Korea Republic of Jeju National University School of Medicine Korea Republic of

Most HIFU procedures use the thermal effect of ultrasound, and information about temperature is directly related to the effect and side effects of the procedure. In the case of the human body, there are complex structures including bones around the target tissue to be irradiated with ultrasound. Thermal lesions in human tissue differ from thermal lesions in ideal conditions due to the standing waves generated by the target tissue and surrounding structures. If a high temperature rise is induced at a location other than the focal point of the HIFU, side effects of the procedure and patient safety may be threatened. In this study, two conditions were simulated, a tissue-mimicking phantom and a human body, in order to compare differences in thermal lesions. In the simulation results, the shape of the thermal lesion generated in the two conditions was not the same, and there was a difference in the location where the temperature was increased. © ICA *** rights reserved

关键词： HIFU Human phantom Thermal lesion Tissue mimicking phantom

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VR-Based Implicit Authentication Using Electromyogram and Inertial Measurement Unit 23

VR-Based Implicit Authentication Using Electromyogram and In...

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23rd International Conference on Cyberworlds, CW 2024

作者： Yu, Da-Young Lee, Hyung-Tak Kim, Youngsam Roh, Jong-Hyuk Kim, Soohyung Ku, Jeonghun Hwang, Han-Jeong Korea University Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology Dept. Electronics and Information Engineering Sejong Korea Republic of Korea University Dept. Electronics and Information Engineering Sejong Korea Republic of Daejeon Korea Republic of Keimyung University College of Medicine Department of Biomedical Engineering Daegu Korea Republic of

ISBN: (纸本)9798331527174

Biometrics based on electromyogram (EMG) and inertial measurement unit (IMU) signals has received significant attention due to its resistance to forgery and tampering, as well as its relatively higher convenience and security compared to other biometric modalities. However, most EMG and IMU signal-based authentication methods require explicit authentication using unnatural gestures, which is not userfriendly. To address this, we collected EMG and IMU data during virtual reality (VR) rhythm game play, which does not require explicit gestures, and performed implicit authentication. Using a linear discriminant analysis (LDA) classifier, we achieved classification accuracies of 91.71% for human identification and 87.91% for human authentication. The result demonstrated the feasibility of a seamless and implicit authentication in VR environments. © 2024 IEEE.

关键词： Electromyography

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Bioceramic-mediated chondrocyte hypertrophy promotes calcified cartilage formation for rabbit osteochondral defect repair

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Bioactive Materials 2024年第10期40卷 306-317页

作者： Rachel H.Koh Junhee Kim Jeong-Uk Kim Seunghyun L.Kim Arun Kumar Rajendran Seunghun SLee Heesoo Lee Joo Hyun Kim Ji Hoon Jeong Yongsung Hwang Jong Woo Bae Nathaniel S.Hwang School of Chemical and Biological Engineering Institute of Chemical ProcessesSeoul National UniversitySeoul08826South Korea Interdisciplinary Program in Bioengineering Seoul National UniversitySeoul08826South Korea Department of Biomedical Engineering Dongguk UniversitySeoul10326South Korea Soonchunhyang Institute of Medi-bio Science(SIMS) Soonchunhyang UniversityCheonan31151South Korea Department of Integrated Biomedical Science Soonchunhyang UniversityAsan31538South Korea Department of Orthopaedic Surgery Konkuk University Chungju HospitalKonkuk University School of MedicineChungju27376South Korea BioMAX Institute Seoul National UniversitySeoul08826South Korea

Osteochondral tissue is a highly specialized and complex tissue composed of articular cartilage and subchondral bone that are separated by a calcified cartilage *** or gradient scaffolds,often in conjunction with stem cells and growth factors,have been developed to mimic the respective layers for osteochondral defect *** this study,we designed a hyaline cartilage-hypertrophic cartilage bilayer graft(RGD/RGDW)with ***,we demonstrated that RGD peptide-modified chondroitin sulfate cryogel(RGD group)is chondro-conductive and capable of hyaline cartilage ***,we incorporated whitlockite(WH),a Mg^(2+)-containing calcium phosphate,into RGD cryogel(RGDW group)to induce chondrocyte hypertrophy and form collagen X-rich hypertrophic *** is the first study to use WH to produce hypertrophic ***-laden RGDW cryogel exhibited significantly upregulated expression of hypertrophy markers in vitro and formed ectopic hypertrophic cartilage in vivo,which mineralized into calcified cartilage in bone ***,RGD cryogel and RGDW cryogel were combined into bilayer(RGD/RGDW group)and implanted into rabbit osteochondral defect,where RGD layer supports hyaline cartilage regeneration and bioceramic-containing RGDW layer promotes calcified cartilage *** the RGD group(monolayer)formed hyaline-like neotissue that extends into the subchondral bone,the RGD/RGDW group(bilayer)regenerated hyaline cartilage tissue confined to its respective layer and promoted osseointegration for integrative defect repair.

关键词： Bioceramic Whitlockite Bilayer scaffold Chondrocyte hypertrophy Osteochondral defect repair

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Evaluating Crosslinking Efficiency and Cytocompatibility of Three Commonly Used Photoinitiators Across Different Cell-Compatible Hydrogel Platforms

SSRN

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SSRN 2025年

作者： Gavish Moscovitz, Aya Simaan Yameen, Haneen Bar-Am, Orit Seliktar, Dror The Faculty of Biomedical Engineering Technion-Israel Institute of Technology Haifa Israel The Interdisciplinary Program in Biotechnology Technion–Israel Institute of Technology Haifa Israel

biomedical hydrogels that undergo a rapid liquid-to-solid phase transition often use a photopolymerization strategy to crosslink the polymer network. In applications such as 3D bioprinting or tissue engineering that involve cell-laden hydrogels, or where in-vivo polymerization is desired (i.e., in-situ), cytotoxicity of the hydrogel crosslinking reaction becomes an important issue. There are only a few cytocompatible photoinitiators (PIs) that are commonly used for crosslinking biomedical hydrogels, including Irgacure2959 (I2959), lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and EosinY (EY). These PIs are illuminated at 365nm, 405nm and 525nm, respectively. The photopolymerization conditions, including PI concentration and illumination intensity may affect both the cytotoxicity of the reaction and the crosslinking of the hydrogel. Herein we tested these three PIs to optimize the maximum crosslinking efficiency while minimizing cell death associated with the photochemical reaction. Testing was performed on three different types of cell-compatible hydrogels used for cell encapsulation and 3D cell growth, including a synthetic material (poly(ethylene glycol)-diacrylate, PEG-DA), a semi-synthetic material (PEG-fibrinogen, PF) and a modified biological material (methacrylated fibrinogen, FibMA). Strain-rate controlled rheology was used to characterize the crosslinking reaction efficiency, both in terms of maximum shear storage modulus, G’, and crosslinking kinetics. Viability was examined on human dermal fibroblasts in 3D culture within photopolymerized PF hydrogels crosslinked using each of the PIs. The results showed that PI concentration and the illumination intensity had significant impact on the crosslinking efficiency, with each material system demonstrating different responses to the PI parameters. Optimal photo-crosslinking conditions, considering both crosslinking efficiency and cell viability were not the same for the modified protein hydrogels, as co

关键词： Cell death

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Development of exosome membrane materialsfused microbubbles for enhanced stability and efficient drug delivery of ultrasound contrast agent

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Acta Pharmaceutica Sinica B 2023年第12期13卷 4983-4998页

作者： Yongho Jang Jeehun Park Pilsu Kim Eun-Joo Park Hyungjin Sun Yujin Baek Jaehun Jung Tai-kyong Song Junsang Doh Hyuncheol Kim Department of Chemical and Biomolecular Engineering Sogang UniversitySeoul 04107Republic of Korea Research Institute of Advanced Materials(RIAM) Institute of Engineering ResearchSeoul National UniversitySeoul 08826Republic of Korea Department of Electronic Engineering Sogang UniversitySeoul 04107Republic of Korea Biomedical Research Institute&Department of Radiology Seoul National University HospitalSeoul 03080Republic of Korea Interdisciplinary Program in Bioengineering Seoul National UniversitySeoul 08826Republic of Korea Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Republic of Korea Institute of Engineering Research Bio-MAX InstituteSeoul National UniversitySeoul 08826Republic of Korea Department of Biomedical Engineering Sogang UniversitySeoul 04107Republic of Korea

Lipid-coated microbubbles are widely used as an ultrasound contrast agent,as well as drug delivery ***,the two main limitations in ultrasound diagnosis and drug delivery using microbubbles are the short half-life in the blood system,and the difficulty of surface modification of microbubbles for active *** exosome,a type of extracellular vesicle,has a preferentially targeting ability for its original *** this study,exosome-fused microbubbles(Exo-MBs)were developed by embedding the exosome membrane proteins into *** a result,the stability of Exo-MBs is improved over the conventional *** the same principle that under the exposure of ultrasound,microbubbles are cavitated and self-assembled into nano-sized particles,and Exo-MBs are self-assembled into exosome membrane proteins-embedded nanoparticles(Exo-NPs).The Exo-NPs showed favorable targeting properties to their original cells.A photosensitizer,chlorin e6,was loaded into Exo-MBs to evaluate therapeutic efficacy as a drug *** higher therapeutic efficacy of photodynamic therapy was confirmed,followed by cancer immunotherapy from immunogenic cell *** have therefore developed a novel ultrasound image-guided drug delivery platform that overcomes the shortcomings of the conventional ultrasound contrast agent and is capable of simultaneous photodynamic therapy and cancer immunotherapy.

关键词： Exosomes Microbubbles Photodynamic therapy Ultrasound Sonoporation Cancer immunotherapy Drug delivery

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Injectable Platelet Lysate–Thai Silk Fibroin Hydrogel for Canine Intervertebral Disc Repair: A Minimally Invasive Strategy for Sustained Growth Factor Release

SSRN

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SSRN 2025年

作者： Ratanavaraporn, Juthamas Chayatup, Kannika Boonkruephan, Sarinthorn Keeratihattayakorn, Saran Wangdee, Chalika Interdisciplinary Program of Biomedical Engineering Program Faculty of Engineering Chulalongkorn University Bangkok Thailand Biomedical Engineering Research Center Faculty of Engineering Chulalongkorn University Bangkok Thailand Research Unit Chulalongkorn University Bangkok10330 Thailand Department of Veterinary Surgery Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand

Canine intervertebral disc degeneration (IVDD) is a progressive spinal disorder that compromises mobility, induces chronic pain, and reduces quality of life. Existing treatments provide only symptomatic relief and do not halt disease progression or restore disc integrity. This study aims to develop and characterize an injectable platelet lysate–Thai silk fibroin (PL–TSF) hydrogel as a minimally invasive platform for localized and sustained growth factor delivery in canine IVDD treatment. The hydrogel was formulated using PL from expired canine platelet concentrates and TSF extracted from cocoons of Thai-bred Bombyx mori silkworms. Fabricated via sonication-induced gelation, it exhibited tunable gelation kinetics and injectability through a 27-gauge spinal needle, supporting clinical feasibility for minimally invasive delivery. PL incorporation enhanced the hydrogel’s elasticity and mechanical resilience without compromising scaffold integrity. The system demonstrated biphasic degradation over 35 days. TGF-β1 release kinetics exhibited an initial burst followed by sustained release over 28 days, consistent with diffusion-controlled delivery suitable for IVD repair. Cytotoxicity testing confirmed good biocompatibility and supported the viability of canine chondrocytes. By combining mechanical support with prolonged growth factor availability, PL–TSF hydrogels address key limitations of conventional platelet-based therapies and represent a promising strategy for early-stage IVDD intervention. Further in vivo studies are warranted to confirm long-term regenerative efficacy and facilitate clinical translation in veterinary medicine. © 2025, The Authors. All rights reserved.

关键词： Gelation

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