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Differences in creep response of GBM cells migrating in confinement

International Biomechanics 2020年第1期7卷 44-57页

作者： Khan, Ishan Bui, Loan Bachoo, Robert Kim, Young-Tae Chuong, Cheng-Jen University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas Joint Graduate Program in Biomedical Engineering United States Department of Neurology & Neurotherapeutics University of Texas Southwestern Medical Center at Dallas Dallas TX United States University of Texas at Arlington Department of Bioengineering TX United States

Using a microfluidic platform to apply negative aspiration pressure (–20, –25, –30, –35 and –40 cm H2O), we compared the differences in creep responses of Glioblastoma Multiforme (GBM) cells while migrating in co... 详细信息

Using a microfluidic platform to apply negative aspiration pressure (–20, –25, –30, –35 and –40 cm H₂O), we compared the differences in creep responses of Glioblastoma Multiforme (GBM) cells while migrating in confinement and at a stationary state on a 2D substrate. Cells were either migrating in a channel of 5 x 5 μm cross-section or stationary at the entrance to the channel. In response to aspiration pressure, we found actively migrating GBM cells exhibited a higher stiffness than stationary cells. Additionally, migrating cells absorbed more energy elastically with a relatively small dissipative energy loss. At elevated negative pressure loads up to–30 cm H₂O, we observed a linear increase in elastic deformation and a higher distribution in elastic storage than energy loss, and the response plateaued at further increasing negative pressure loads. To explore the underlying cause, we carried out immuno-cytochemical studies of these cells and found a polarized actin and myosin distribution at the front and posterior ends of the migrating cells, whereas the distribution of the stationary group demonstrated no specific regional differences. These differences in creep response and cytoskeletal protein distribution demonstrate the importance of a migrating cell’s kinematic state to the mechanism of cell migration. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

关键词： actomyosin contraction Cancer cell creep glioblastoma viscoelastic properties

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Biomimetic in silico devices

Biomimetic in silico devices

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International Conference on Computational Methods in Systems Biology, CMSB 2004

作者： Hunt, C. Anthony Ropella, Glen E. P. Roberts, Michael S. Yan, Li Dept. of Biopharmaceutical Sciences Biosystems Group University of California San Francisco CA 94143-0446 United States Department of Medicine University of Queensland Princess Alexandra Hospital Woolloongabba Q 4102 Australia Joint UCSF UC Berkeley Bioengineering Graduate Program

ISBN: (纸本)3540253750

We introduce biomimetic in silico devices, and means for validation along with methods for testing and refining them. The devices are constructed from adaptable software components designed to map logically to biological components at multiple levels of resolution. In this report we focus on the liver;the goal is to validate components that mimic features of the lobule (the hepatic primary functional unit) and dynamic aspects of liver behavior, structure, and function. An assembly of lobule-mimetic devices represents an in silico liver. We validate against outflow profiles for sucrose administered as a bolus to isolated, perfused rat livers. Acceptable in silico profiles are experimentally indistinguishable from those of the in situ referent. This new technology is intended to provide powerful new tools for challenging our understanding of how biological functional units function in vivo. © Springer-Verlag Berlin Heidelberg 2005.

关键词： Biomimetic materials

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The effect of Cytochalasin D on F-actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

The effect of Cytochalasin D on F-actin behavior of single-c...

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7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

作者： Lin, Ran Chang, Donald. C. Lee, Yi-Kuen Bioengineering Graduate Program HKUST Kowloon Hong Kong Dept. of Mechanical Engineering HKUST Kowloon Hong Kong Division of Life Science HKUST Kowloon Hong Kong Joint KAUST-HKUST Micro/Nanofluidics Laboratory HKUST Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)9781467311243

Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (CCD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and CCD. © 2012 IEEE.

关键词： Electric fields

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Spectral DiffuserScope - Computational Snapshot Hyperspectral Imager for Microscopy

Spectral DiffuserScope - Computational Snapshot Hyperspectra...

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2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023

作者： Aggarwal, Neerja Markley, Eric Raniwala, Yashovardhan Houck, William D. Lee, Kyung Chul Bae, Junghyun Baek, Nakkyu Park, Wook Lee, Seung Ah Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States VIAVI Solutions Inc Santa Rosa United States School of Electrical and Electronic Engineering Yonsei University Seoul Korea Republic of

We introduce a computational snapshot hyperspectral imager for benchtop microscopy. The compact device uses a diffuser and spectral filter array to multiplex spatio-spectral information into a 2D measurement, then rec... 详细信息

ISBN: (纸本)9781957171289

关键词： Blue light emitting diodes Diffusers Fluorescence microscopy Image sensors Spatial resolution Ultraviolet light emitting diodes

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Diffuse optical tomography to study ischemic stroke in preclinical rat models

Diffuse optical tomography to study ischemic stroke in precl...

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Biomedical Optics, BIOMED 2012

作者： Lin, Zi-Jing Ren, Ming Li, Lin Yang, Shaohua Liu, Hanli Department of Bioengineering Joint Graduate Program b/w Univ. of Texas Southwestern Medical University of Texas TX 76019 United States Department of Pharmacology and Neuroscience Institute for Aging and Alzheimer's Disease Research University of North Texas Health Science Center Fort Worth TX 76107 United States

We investigated hyper-acute cerebral ischemia using diffuse optical tomography with the depth compensation algorithm (DCA) in pre-clinical rat models. Results suggest that this methodology has a great potential to mon... 详细信息

ISBN: (纸本)9781557529428

关键词： Rats

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Physics-based learned design for Fourier DiffuserScope

Physics-based learned design for Fourier DiffuserScope

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Computational Optical Sensing and Imaging, COSI 2021 - Part of OSA Imaging and Applied Optics Congress 2021

作者： Markley, Eric Liu, Fanglin Linda Kellman, Michael Antipa, Nick Waller, Laura UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States Department of Electrical Engineering and Computer Sciences University of California Berkeley United States Department of Pharmaceutical Chemistry University of California San Francisco United States Department of Electrical and Computer Engineering University of California San Diego United States

We couple a differentiable forward model of diffuser-based single-shot 3D microscopy (Fourier DiffuserScope) with an invertible and differentiable reconstruction algorithm to jointly optimize the imaging system and th... 详细信息

ISBN: (纸本)9781557528209

关键词： Imaging systems

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Fabrication of charged nanopore channels using pulsed plasma deposition of methacrylic acid

Fabrication of charged nanopore channels using pulsed plasma...

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2011 11th IEEE International Conference on Nanotechnology, NANO 2011

作者： Asghar, Waseem Ilyas, Azhar Timmons, Richard B. Iqba, Samir M. Electrical Engineering Department Nanotechnology Research and Teaching Facility University of Texas Arlington TX 76019 United States Department of Chemistry and Biochemistry University of Texas Arlington TX 76019 United States Joint Graduate Committee of Bioengineering Program Medical Center at Dallas University of Texas at Arlington Arlington TX 76019 United States

ISBN: (纸本)9781457715143

Solid-state nanopore systems have emerged as novel platforms for DNA, RNA and protein analysis. In the state of the art, the nanopores are made using electron beam induced shrinking of larger pores, drilling in thin membranes or deposition of material on fabricated nanopores. These processes have slow deposition rates, provide poor control of surface composition of deposited layers and the membrane thickness defines whether a pore shrinks or expands. This paper reports a novel technique of pulsed plasma assisted polymer film deposition that offers a direct way to control shrinking of the nanopores and to impart defined surface properties to the nanopore walls. © 2011 IEEE.

关键词： Nanopores

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Layered Diffusion Model for One-Shot High Resolution Text-to-Image Synthesis

arXiv

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arXiv 2024年

作者： Khwaja, Emaad Rashwan, Abdullah Chen, Ting Wang, Oliver Kothawade, Suraj Li, Yeqing UC Berkeley UCSF Joint Bioengineering Graduate Program United States Computer Science Division UC Berkeley United States Google United States Google Deepmind United Kingdom

We present a one-shot text-to-image diffusion model that can generate high-resolution images from natural language descriptions. Our model employs a layered U-Net architecture that simultaneously synthesizes images at multiple resolution scales. We show that this method outperforms the baseline of synthesizing images only at the target resolution, while reducing the computational cost per step. We demonstrate that higher resolution synthesis can be achieved by layering convolutions at additional resolution scales, in contrast to other methods which require additional models for super-resolution synthesis. © 2024, CC BY.

关键词： Diffusion

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Fourier diffuserscope: Single-shot 3d fourier light field microscopy with a diffuser

arXiv

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arXiv 2020年

作者： Liu, Fanglin Linda Grace, K.U.O. Antipa, Nick Yanny, Kyrollos Waller, Laura Electrical Engineering and Computer Sciences University of California Berkeley94709 United States UCB/UCSF Joint Graduate Program in Bioengineering University of California BerkeleyCA94709 United States

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample, without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of lateral resolution at the focal plane, which is highly undesirable in microscopy. Placing the MLA near the pupil plane of the microscope, rather than the image plane, can mitigate the artifacts near focus and provide an efficient shift-invariant model at the expense of field-of-view. Here, we show that our Fourier DiffuserScope achieves significantly better performance than Fourier LFM. Fourier DiffuserScope uses a diffuser in the pupil plane to encode depth information, then reconstructs volumetric information computationally by solving a sparsity-constrained inverse problem. Our diffuser consists of randomly placed microlenses with varying focal lengths. We show that by randomizing the microlens positions, a larger lateral field-of-view can be achieved compared to a conventional MLA;furthermore, by adding diversity to the focal lengths, the axial depth range is increased. To predict system performance based on diffuser parameters, we for the first time establish a theoretical framework as a design guideline, followed by numerical simulations to verify. Under both theoretical and numerical analysis, we demonstrate that our diffuser design provides more uniform resolution over a larger volume, both laterally and axially, outperforming the MLA used in LFM. We build an experiment system with an optimized lens set and achieve 3 volume. Copyright © 2020, The Authors. All rights reserved.

关键词： Microlenses

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Vapor-phase facile coatings of nanotextured organic biocompatible films on solid-state substrates

Vapor-phase facile coatings of nanotextured organic biocompa...

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作者： Goyal, Swati Kim, Young-Tae Iqbal, Samir M. Life Technologies Corporation CA 92008 United States Department of Bioengineering Nanotechnology Research and Teaching Facility University of Texas Arlington Arlington TX 76019 United States Department of Electrical Engineering Nanotechnology Research and Teaching Facility University of Texas Arlington Arlington TX 76019 United States Joint Graduate Studies Committee Bioengineering Program University of Texas United States Arlington and University of Texas Southwestern Medical Center at Dallas University of Texas Arlington Arlington TX 76019 United States

Fluorinated coatings of solid surfaces are important for many applications ranging from corrosion resistance to low surface energy biological interfaces. We present a facile approach to coat solid-state surfaces directly from vapor phase without harsh chemical or plasma treatments. The coatings show nanostructures with high surface area, which is important for variety of surface functionalizations, for example, in molecule attachment and cell growth. The novel polymeric nanoporous film is achieved from the reaction and deposition of two molecules. The surface morphology and pore sizes of the coating on solid-state surfaces can be tuned with change in concentration of monomers in a simple reaction chamber. The X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy analyses show organic nature of the coating. Cell growth studies are done to gauge biocompatibility and adsorption of proteins and cells. The approach can be used to coat, functionalize, and treat nano- and microstructures for a variety of applications with minimal chemical footprint.© 2010 IEEE.

关键词： Nanostructures

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