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Regulation of vascular smooth muscle cells and mesenchymal stem cells by mechanical strain

MCB Molecular and Cellular Biomechanics 2006年第1期3卷 21-34页

作者： Kurpinski, Kyle Park, Jennifer Thakar, Rahul G. Li, Song Department of Bioengineering University of California at Berkeley Berkeley CA United States Joint Graduate Program in Bioengineering UC Berkeley Berkeley CA United States Joint Graduate Program in Bioengineering UC San Francisco San Francisco CA United States

Vascular smooth muscle cells (SMCs) populate in the media of the blood vessel, and play an important role in the control of vasoactivity and the remodeling of the vessel wall. Blood vessels are constantly subjected to hemodynamic stresses, and the pulsatile nature of the blood flow results in a cyclic mechanical strain in the vessel walls. Accumulating evidence in the past two decades indicates that mechanical strain regulates vascular SMC phenotype, function and matrix remodeling. Bone marrow mesenchymal stem cell (MSC) is a potential cell source for vascular regeneration therapy, and may be used to generate SMCs to construct tissue-engineered vascular grafts for blood vessel replacements. In this review, we will focus on the effects of mechanical strain on SMCs and MSCs, e.g., cell phenotype, cell morphology, cytoskeleton organization, gene expression, signal transduction and receptor activation. We will compare the responses of SMCs and MSCs to equiaxial strain, uniaxial strain and mechanical strain in three-dimensional culture. Understanding the hemodynamic regulation of SMC and MSC functions will provide a basis for the development of new vascular therapies and for the construction of tissue-engineered vascular, grafts. Copyright © 2006 Tech Science Press.

关键词： Muscle

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Connectivity of single neurons classifies cell subtypes in mouse brains

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Nature Methods 2025年第4期22卷 861-873页

作者： Liu, Lijuan Yun, Zhixi Manubens-Gil, Linus Chen, Hanbo Xiong, Feng Dong, Hongwei Zeng, Hongkui Hawrylycz, Michael Ascoli, Giorgio A. Peng, Hanchuan New Cornerstone Science Laboratory SEU-ALLEN Joint Center Institute for Brain and Intelligence Southeast University Nanjing China School of Biological Science & Medical Engineering Southeast University Nanjing China Tencent AI Lab Bellevue WA United States UCLA Brain Research and Artificial Intelligence Nexus Department of Neurobiology David Geffen School of Medicine University of California Los Angeles Los Angeles CA United States Allen Institute for Brain Science Seattle WA United States Center for Neural Informatics Bioengineering Department and Neuroscience Program Krasnow Institute for Advanced Study George Mason University Fairfax VA United States Shanghai Academy of Natural Sciences Fudan University Shanghai China

Classification of single neurons at a brain-wide scale is a way to characterize the structural and functional organization of brains. Here we acquired and standardized a large morphology database of 20,158 mouse neurons and generated a potential connectivity map of single neurons based on their dendritic and axonal arbors. With such an anatomy–morphology–connectivity mapping, we defined neuron connectivity subtypes for neurons in 31 brain regions. We found that cell types defined by connectivity show distinct separation from each other. Within this context, we were able to characterize the diversity in secondary motor cortical neurons, and subtype connectivity patterns in thalamocortical pathways. Our findings underscore the importance of connectivity in characterizing the modularity of brain anatomy at the single-cell level. These results highlight that connectivity subtypes supplement conventionally recognized transcriptomic cell types, electrophysiological cell types and morphological cell types as factors to classify cell classes and their identities. © The Author(s) 2025.

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Acromion and Scapular Spine Fractures in Reverse Total Shoulder Arthroplasty An XFEM Study

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Journal of shoulder and elbow surgery 2025年

作者： Shuchun Sun Jian Chen Farhad Ahmadi Jichao Zhao Josef K Eichinger Brandon L Rogalski Hai Yao Richard J Friedman Clemson-MUSC Joint Bioengineering Program Clemson University Clemson SC USA. Department of Orthopedics and Physical Medicine Medical University of South CarolinaCharlestonSCUSA. Clemson-MUSC Joint Bioengineering Program Clemson University Clemson SC USA Department of Orthopedics and Physical Medicine Medical University of South CarolinaCharlestonSCUSA. Electronic address: friedman@musc.edu.

BACKGROUND:Reverse total shoulder arthroplasty (rTSA) is widely used to restore shoulder function and alleviate pain. In approximately 1-4% of patients, acromion and scapular spine fractures (ASF) occur and present a clinical challenge for orthopedic surgeons. While impact trauma, impingement, and excessive muscle forces are proposed causes, the relationship between these loading conditions and ASF types remains unclear, hindering targeted prevention strategies. The objective of this study is to identify the biomechanical relationship between loading conditions and ASF types, with the goal of informing targeted preventive strategies to reduce fracture incidence. METHODS:An extended finite element model (XFEM) was developed using computed tomography (CT)-derived shoulder geometry from virtual surgeries to simulate ASF. Bone material properties were based on CT Hounsfield units (HU). Loading scenarios, including impact trauma, impingement, and excessive deltoid force, were informed by existing literature. Simulations were performed in Abaqus, with mesh independence testing ensuring result accuracy. Fracture locations were analyzed according to the modified Levy ASF classification. RESULTS:For impact traumas, the analysis identified that fractures occurred primarily at the initial contact point of the acromion, predominantly in the lateral region. Specifically, forward and sideway impact traumas typically resulted in modified Levy Type I ASF, while backward impact traumas could produce modified Levy Type IIA ASF. Lateral impingement typically led to lateral fractures, specifically modified Levy Type I ASF. The effects of deltoid force varied with the type of shoulder activity; excessive movements during shoulder abduction and forward flexion could cause modified Levy Type IIA ASF. In contrast, hyperextension of the shoulder could shift the fracture location to the medial region, leading to modified Levy Type III ASF. CONCLUSION:Impact trauma, impingement, and excessive

关键词： Acromion and Spine Fractures Biomechanics Extended Finite Element Analysis Reverse Total Shoulder Arthroplasty

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A DUAL-MODALITY OPTICAL BIOPSY APPROACH FOR IN VIVO DETECTION OF PROSTATE CANCER IN RAT MODEL

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Journal of Innovative Optical Health Sciences 2011年第3期4卷 269-277页

作者： VIKRANT SHARMA NIMIT PATEL JINHUI SHEN LIPING TANG GEORGE ALEXANDRAKIS HANLI LIU Department of Bioengineering Joint Graduate Program between University of Texas at Arlington and University of Texas Southwestern Medical Center University of Texas at Arlington TX 76019USA

Ultrasound-guided biopsy procedure for prostate cancer diagnosis,which is the current gold standard,involves quasi-random sampling of prostate tissue without any functional *** this study,we discuss the possibility to augment the detection of prostate cancer using a dual-modality optical approach,which can be coupled with the current needle biopsy *** techniques are light reflectance spectroscopy(LRS)that uses a broadband light source and a CCD array spectrometer,and auto-fluorescence lifetime measurement(AFLM)that uses a custom-designed,time-correlated single photon counting(TCSPC)*** LRS and AFLM were employed sequentially in this study to measure cancer tissue along with control tissue on a rat prostate tumor *** an excitation wavelength of 447 nm,we investigated auto-fluorescence decay curves at the emission wavelengths of 532,562,632 and 684 nm for in vivo and ex vivo *** results show that auto-fluorescence lifetimes at all measured emission wavelengths differ between control and cancerous tissues with 100% specificity and ***,absolute values of hemoglobin derivatives and scattering coe±cient were quantified using in vivo *** part of study also demonstrates that light scattering and absorption are significantly different between the control and cancerous ***,the study demonstrates that both LRS and AFLM may provide several intrinsic biomarkers for in vivo detection of prostate cancer.

关键词： Prostate cancer light reflectance spectroscopy auto-fluorescence lifetime

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Cell-mediated and cell membrane-coated nanoparticles for drug delivery and cancer therapy

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Cancer Drug Resistance 2020年第4期3卷 879-911页

作者： Serkan Yaman Uday Chintapula Edgar Rodriguez Harish Ramachandramoorthy Kytai T.Nguyen Department of Bioengineering University of Texas at ArlingtonArlingtonTX 76010USA Joint Bioengineering Program University of Texas Southwestern Medical CenterDallasTX 75235USA

Nanotechnology-based drug delivery platforms have been developed over the last two decades because of their favorable features in terms of improved drug bioavailability and *** recent advancement in nanotechnology platforms,this approach still falls short to meet the complexity of biological systems and diseases,such as avoiding systemic side effects,manipulating biological interactions and overcoming drug resistance,which hinders the therapeutic outcomes of the NP-based drug delivery *** address these issues,various strategies have been developed including the use of engineered cells and/or cell membrane-coated *** membrane receptor profiles and characteristics are vital in performing therapeutic functions,targeting,and homing of either engineered cells or cell membrane-coated nanocarriers to the sites of *** this context,we comprehensively discuss various cell-and cell membrane-based drug delivery approaches towards cancer therapy,the therapeutic potential of these strategies,and the limitations associated with engineered cells as drug carriers and cell membrane-associated drug ***,we review various cell types and cell membrane receptors for their potential in targeting,immunomodulation and overcoming drug resistance in cancer.

关键词： Cell membrane-based drug delivery cell-mediated drug delivery membrane receptors drug carriers cancer drug resistance nanoparticles

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In vivo tumor ultrasound-switchable fluorescence imaging via intravenous injections of size-controlled thermosensitive nanoparticles

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Nano Research 2023年第1期16卷 1009-1020页

作者： Liqin Ren Yang Liu Tingfeng Yao Kytai TNguyen Baohong Yuan Ultrasound and Optical Imaging Laboratory Department of Bioengineeringthe University of Texas at ArlingtonArlingtonTX 76019USA Joint Biomedical Engineering Program the University of Texas at Arlington and the University of Texas Southwestern Medical CenterDallasTX 75390USA Department of Bioengineering the University of Texas at ArlingtonArlingtonTX 76019USA

Near-infrared fluorescence imaging has emerged as a noninvasive,inexpensive,and ionizing-radiation-free monitoring tool for assessing tumor growth and treatment *** particular,ultrasound switchable fluorescence(USF)imaging has been explored with improved imaging sensitivity and spatial resolution in centimeter-deep *** study achieved the size control of polymer-based and indocyanine green(ICG)encapsulated USF contrast agents,capable of accumulating in the tumor after intravenous *** nanoprobes varied in size from 58 to 321 *** bioimaging profiles demonstrated that the proposed nanoparticles can efficiently eliminate the background light from normal tissue and show a tumor-specific fluorescence enhancement in the BxPC-3 tumor-bearing mice models possibly via the enhanced permeability and retention *** vivo tumor USF imaging further demonstrated that these nanoprobes can effectively be switched“ON”with enhanced fluorescence in response to a focused ultrasound stimulation in the tumor microenvironment,contributing to the high-resolution USF ***,our findings suggest that ICG-encapsulated nanoparticles are good candidates for USF imaging of tumors in live animals,indicating their great potential in optical tumor imaging in deep tissue.

关键词： in vivo tumor imaging deep tissue near-infrared(NIR)imaging high resolution ultrasound-switchable fluorescence imaging indocyanine green(ICG)

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Temperature-sensitive polymeric nanogels encapsulating withβ-cyclodextrin and ICG complex for high-resolution deep-tissue ultrasound-switchable fluorescence imaging

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Nano Research 2020年第4期13卷 1100-1110页

作者： Ruilin Liu Tingfeng Yao Yang Liu Shuai Yu Liqin Ren Yi Hong Kytai T.Nguyen Baohong Yuan Ultrasound and Optical Imaging Laboratory Department of Bioengineeringthe University of Texas at ArlingtonArlingtonTX 76019USA Joint Biomedical Engineering Program the University of Texas at Arlington and the University of Texas Southwestern Medical CenterDallasTX 75390USA Department of Bioengineering the University of Texas at ArlingtonArlingtonTX 76019USA

One of the thorny problems currently impeding the applications of the fluorescence imaging technique is the poor spatial resolution in deep ***-switchable fluorescence(USF)imaging is a novel imaging tool that has recently been explored to possibly surmount the above-mentioned ***,αβ-cyclodextrin/indocyanine green(ICG)complex-encapsulated poly(N-isopropylacrylamide)(PNIPAM)nanogel was synthesized and studied for ex vivo/in vivo deep tissue/high-resolution near infrared USF(NIR-USF)*** be specific,our results revealed that the average diameter of the as-prepared nanogels was significantly decreased to-32 nm from-335 nm compared to the reported ICG-PNIPAM ***,the excitation/emission characteristics of the ICG itself in present nanogels were almost completely retained,and the resultant nanogel exhibited high physiological stability and positive *** particular,the signal-to-noise ratio of the USF image for the PNIPAM/P-cyclodextrin/ICG nanogel(33.01±2.42 dB)was prominently higher than that of the ICG-PNIPAM nanoparticles(18.73±0.33 dB)in 1.5-cm-thick chicken breast *** NIR-USF imaging in 3.5-cm-thick chicken breast tissues was achieved using this new *** e x v iv o NIR-USF imaging of the mouse liver was also successfully *** experiments showed that the present nanogels were able to be effectively accumulated into U87 tumor-bearing mice via enhanced permeability and retention effects,and the high-resolution NIR-USF imaging of in v ivo tumor was efficiently *** metabolism and in vivo biodistribution of the nanogels were ***,the results suggest that the current nanogel is a highly promising NIR-USF probe for deep tissue and high-resolution USF imaging.

关键词： deep tissue imaging high-resolution ultrasound-switchable NIR fluorescence imaging in vivo imaging

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High-density spheroid arrays for 3-D liver cell culture and secretion analysis

High-density spheroid arrays for 3-D liver cell culture and ...

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10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

作者： Zhang, Mimi Y. Di Carlo, Dino Wu, Liz Y. Lee, Luke P. Berkeley Sensor and Actuator Center UCSF/UCB Joint Program in Bioengineering University of California Berkeley CA United States

To effectively coalesce otherwise monolayer liver cells (hepatocytes) seeded into microfluidic channels, we present a high-density cell-trapping array chip for rapid three-dimensional hepatocyte spheroid self-assembly. Moreover, by modifying trap geometries, spheroids of varying sizes can be formed in order to optimize the spatial microenvironment for improving cell viability and function. Preliminary sensitivity analysis of albumin secretion detection demonstrates the feasibility of using an optimized chemiluminescent immunoassay to determine the albumin concentrations in the range of 1 ng/mL to 500 ng/mL. © 2006 Society for Chemistry and Micro-Nano Systems.

关键词： Cell culture

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Absolute quantification of hemoglobin derivative concentrations and reduced scattering coefficients from turbid media using steady state reflectance spectroscopy with single source-detector separation

Absolute quantification of hemoglobin derivative concentrati...

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Biomedical Topical Meeting, BIO 2006

作者： kashyap, Dheerendra Liu, Hanli Joint Program of Bioengineering University of Texas at Arlington Southwestern Medical Center at Dallas Arlington TX-76019-0138 United States

Steady state broadband (650-845nm) reflectance spectroscopy yields absolute concentrations of hemoglobin derivatives and reduced scattering coefficients of blood-intralipid phantoms within 15% relative error, using an... 详细信息

ISBN: (纸本)1557528071

关键词： Hemoglobin

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Targeted chemotherapy via HER2-based chimeric antigen receptor(CAR)engineered T-cell membrane coated polymeric nanoparticles

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Bioactive Materials 2024年第4期34卷 422-435页

作者： Serkan Yaman Harish Ramachandramoorthy Priyanka Iyer Uday Chintapula Tam Nguyen Manoj Sabnani Tanviben Kotadia Soroush Ghaffari Laurentiu M.Pop Raquibul Hannan Jon A.Weidanz Kytai T.Nguyen Department of Bioengineering University of Texas at ArlingtonTXUSA Department of Biology University of Texas at ArlingtonTXUSA Joint Bioengineering Program University of Texas Southwestern Medical CenterTXUSA Department of Radiation Oncology University of Texas Southwestern Medical CenterTXUSA Department of Kinesiology University of Texas at ArlingtonArlingtonTXUSA

Cell membrane-derived nanoparticles(NPs)have recently gained popularity due to their desirable features in drug delivery such as mimicking properties of native cells,impeding systemic clearance,and altering foreign body *** NP technology,adoptive immunotherapy has emerged due to its promise in cancer specificity and therapeutic *** this research,we developed a biomimetic drug carrier based on chimeric antigen receptor(CAR)transduced T-cell *** that purpose,anti-HER2 CAR-T cells were engineered via lentiviral transduction of anti-HER2 CAR coding lentiviral ***-HER2 CAR-T cells were characterized by their specific activities against the HER2 antigen and used for cell membrane ***-cancer drug Cisplatin-loaded poly(D,L-lactide-co-glycolic acid)(PLGA)NPs were coated with anti-human epidermal growth factor receptor 2(HER2)-specific CAR engineered T-cell ***-HER2 CAR-T-cell membrane-coated PLGA NPs(CAR-T-MNPs)were characterized and confirmed via fluorescent microscopy and flow ***-coated NPs showed a sustained drug release over the course of 21 days in physiological ***-loaded CAR-T-MNPs also inhibited the growth of multiple HER2+cancer cells in *** addition,in vitro uptake studies revealed that CAR-T-MNPs showed an increased uptake by A549 *** results were also confirmed via in vivo biodistribution and therapeutic studies using a subcutaneous lung cancer model in nude ***-T-MNPs localized preferentially at tumor areas compared to those of other studied groups and consisted of a significant reduction in tumor growth in tumor-bearing *** Conclusion,the new CAR modified cell membrane-coated NP drug-delivery platform has demonstrated its efficacy both in vitro and in ***,CAR engineered membrane-coated NP system could be a promising cell-mimicking drug carrier that could improve therapeutic outcomes of lung cancer treatments.

关键词： Nanoparticles CAR-T cells Membrane-based drug delivery Cancer chemotherapy

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