检索结果-内蒙古大学图书馆

Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2019年第7期25卷 536-542页

作者： PAN Xin XU Kang XIONG Rui CHEN Zhi-min HU Chang-jiang FENG Jian LI Wen-bin College of Pharmacy Chengdu University of Traditional Chinese Medicine Chengdu (610075) China College of Pharmacy South Central University for Nationalities Wuhan (430074) China National Innovation and Attracting Talents "111" Base Bioengineering College Chongqing University Chongqing (400030) China University of California Berkeley-Chongqing University Joint PhD. Student Program in Bioengineering Stanley Hall Berkeley CA (94720) United States Key Research Institute of Quality and Efficacy Evaluation of Traditional Chinese Medicine Formula State Administration of Traditional Chinese Medicine Chengdu (610075) China

Objective: To investigate whether Ershen Pill(ESP,二神丸) could alleviate the symptom of Pi(Spleen)-Shen(Kidney) yang deficiency(PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism. Methods: Seventy-five Sprague-Dawley rats were divided into 5 groups by a random number table, including control, positive, model, low-dose(LD) and high-dose(HD) ESP groups, 15 rats in each group. All the rats, except those in the control group, were developed PSYD induced-diarrhea based on its pathology and etiology. The rats in positive, LD and HD ESP groups were treated with Shenling Baizhu Pill(参苓白术丸), LD(1.05 g/kg) or HD(3.50 g/kg) ESP petroleum ether extract once a day for 2 weeks, respectively. Body weight change and diarrhea index were measured. The histology scores of the kidney were evaluated via hematoxylin and eosin(HE) staining. Aquaporin-3(AQP3) expression in the colon was analyzed by immunofluorescence, quantitative reverse transcription-polymerase chain reaction(qRT-PCR) and Western blot, respectively. Results: Compared with the model group, oral administration of LD and HD ESP prevented body weight loss and inhibited diarrhea after 2-week treatment(P<0.05). Kidney deterioration was impeded, and the histology score in LD and HD ESP groups were 8.2 and 10.5, respectively, which were both higher than those in the model group(P<0.05). In addition, ESP treatment alleviated rat colitis, and HD ESP significantly improved the AQP3 positive staining intensity in the colon tissue compared with the model group. The result from Western blot revealed that AQP3 protein synthesis in colon tissue of LD and HD ESP groups increased by 2.1-and 5.9-fold compared with the model group(P<0.05). qR T-PCR result showed that AQP3 gene expression in the HD ESP group was also up-regulated by 2.5-fold normalized to the model group(P<0.05). Conclusion: ESP extract effectively alleviates the symptoms of PSYD and relieves PSYD-induced diarrhea by improving AQP3 synthesis in the col

关键词： Ershen Pill Chinese medicine petroleum ether extract anti-diarrhea AQP3 colon

Single-nucleus analysis of accessible chromatin in developing mouse forebrain reveals cell-type-specific transcriptional regulation (vol 21, pg 432, 2018)

学校读者我要写书评

暂无评论

NATURE NEUROSCIENCE 2018年第7期21卷 1015-1015页

作者： Preissl, Sebastian Fang, Rongxin Huang, Hui Zhao, Yuan Raviram, Ramya Gorkin, David U. Zhang, Yanxiao Sos, Brandon C. Afzal, Veena Dickel, Diane E. Kuan, Samantha Visel, Axel Pennacchio, Len A. Zhang, Kun Ren, Bing Ludwig Institute for Cancer Research La Jolla CA USA Center for Epigenomics Department of Cellular and Molecular Medicine University of California San Diego School of Medicine La Jolla CA USA Bioinformatics and Systems Biology Graduate Program University of California San Diego La Jolla CA USA Biomedical Sciences Graduate Program University of California San Diego La Jolla CA USA Department of Bioengineering University of California San Diego La Jolla CA USA Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory Berkeley CA USA US Department of Energy Joint Genome Institute Walnut Creek CA USA School of Natural Sciences University of California Merced Merced CA USA Comparative Biochemistry Program University of California Berkeley Berkeley CA USA Institute of Genomic Medicine Moores Cancer Center University of California San Diego School of Medicine La Jolla CA USA

Analysis of chromatin accessibility can reveal transcriptional regulatory sequences, but heterogeneity of primary tissues poses a significant challenge in mapping the precise chromatin landscape in specific cell types. Here we report single-nucleus ATAC-seq, a combinatorial barcoding-assisted single-cell assay for transposase-accessible chromatin that is optimized for use on flash-frozen primary tissue samples. We apply this technique to the mouse forebrain through eight developmental stages. Through analysis of more than 15,000 nuclei, we identify 20 distinct cell populations corresponding to major neuronal and non-neuronal cell types. We further define cell-type-specific transcriptional regulatory sequences, infer potential master transcriptional regulators and delineate developmental changes in forebrain cellular composition. Our results provide insight into the molecular and cellular dynamics that underlie forebrain development in the mouse and establish technical and analytical frameworks that are broadly applicable to other heterogeneous tissues.

关键词： Cell type diversity Chromatin Chromatin analysis Epigenomics

In vivo ultrasound-switchable fluorescence imaging

学校读者我要写书评

暂无评论

arXiv 2018年

作者： Yao, Tingfeng Yu, Shuai Liu, Yang Yuan, Baohong Ultrasound and Optical Imaging Laboratory Department of Bioengineering University of Texas at Arlington ArlingtonTX76010 United States Joint Biomedical Engineering Program University of Texas at Arlington University of Texas Southwestern Medical Center at Dallas DallasTX75235 United States

Ultrasound-switchable fluorescence (USF) imaging was recently developed to overcome the limitation of the poor spatial resolution of the conventional fluorescence imaging in centimeter-deep tissue. However, in vivo USF imaging has not been achieved so far because of the lack of stable near-infrared contrast agents in a biological environment and the lack of data about their biodistributions. In this study, for the first time, we achieved in vivo USF imaging successfully in mice with high resolution. USF imaging in porcine heart tissue and mouse breast tumor via local injections were studied and demonstrated. In vivo and ex vivo USF imaging of the mouse spleen via intravenous injections was also successfully achieved. The results showed that the USF contrast agent adopted in this study was very stable in a biological environment, and it was mainly accumulated into the spleen of the mice. All the results of USF imaging were validated by a commercial micro-CT. Copyright © 2018, The Authors. All rights reserved.

关键词： Fluorescence imaging

Hypoglycemic activity of curcuma mangga val. Extract via modulation of GLUT4 and ppar-γ mrna expression in 3T3-L1 adipocytes

学校读者我要写书评

暂无评论

Journal of Experimental Pharmacology 2020年 12卷 363-369页

作者： Pujimulyani, Dwiyati Yulianto, Wisnu Adi Setyowati, Astuti Arumwardana, Seila Kusuma, Hanna Sari Widya Sholihah, Ika Adhani Rizal, Rizal Widowati, Wahyu Maruf, Ali Faculty of Agroindustry University of Mercu Buana Yogyakarta Yogyakarta 55753 Indonesia Biomolecular and Biomedical Research Center Aretha Medika Utama Bandung 40163 Indonesia Biomedical Engineering Study Program Department of Electrical Engineering Faculty of Engineering University of Indonesia Jakarta 16424 Indonesia Medical Research Center Faculty of Medicine Maranatha Christian University Bandung 40164 Indonesia Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular Implants Bioengineering College Faculty of Medicine Chongqing University Chongqing 400030 China

Background: There would be over 600 million people living with diabetes by 2040 as predicted by the World Health Organization. Diabetes is characterized by raised blood sugar and insulin resistance. Insulin regulates the influx of glucose into the cell by upregulating the glucose transporter type 4 (GLUT4) expression on the plasma membrane. Besides, PPAR-γ also controls the metabolism of glucose in adipose tissues. Curcuma mangga Val., denoted as C. mangga, is a native Indonesian medicinal plant that has many beneficial effects, including an antidiabetic potential. Purpose: In this research, we aimed to disclose the hypoglycemic activity of ethanol extract of C. mangga (EECM) in 3T3-L1 fibroblasts-derived adipocyte cells in regulating glucose uptake as confirmed by the GLUT4 and PPAR-γ gene expression. Methods: The uptake of glucose was determined using radioactive glucose, while the gene expression of GLUT4, PPAR-γ, and β-actin was quantified using mRNA segregation and real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Results: We discovered that EECM interventions (200 and 50 μg/mL) increased glucose uptake in lipid-laden 3T3-L1 cells by 14.75 and 8.86 fold compared to the control noninsulin, respectively (p < 0.05). At the same doses, they also increased GLUT4 mRNA expression by 8.41 and 11.18 fold compared to the control non-insulin, respectively (p < 0.05). In contrast, EECM interventions (200 and 50 μg/mL) showed lower levels of PPAR-γ mRNA expression compared to the control metformin, indicating the anti-adipogenic potentials of EECM. Conclusion: EECM showed hypoglycemic activity in lipid-laden 3T3-L1 cells by improving glucose ingestion into the cells, which was mediated by increased GLUT4 expression and downregulated PPAR-γ expression. © 2020 Pujimulyani et al.

关键词： 3T3-L1 adipocytes Curcuma mangga Val Glucose uptake GLUT4 PPAR-γ

Attenuation coefficient estimation for PET/MRI with Bayesian deep learning pseudo-CT and maximum likelihood estimation of activity and attenuation

学校读者我要写书评

暂无评论

arXiv 2020年

作者： Leynes, Andrew Palmera Ahn, Sangtae P. Wangerin, Kristen A. Kaushik, Sandeep S. Wiesinger, Florian Hope, Thomas A. Larson, Peder E.Z. The Department of Radiology and Biomedical Imaging University of California San Francisco San FranciscoCA94158 United States The UC Berkeley Joint Graduate Program in Bioengineering BerkeleyCA United States UC San Francisco Joint Graduate Program in Bioengineering San FranciscoCA United States GE Research NiskayunaNY United States GE Healthcare WaukeshaWI United States GE Healthcare Munich Germany The Department of Computer Science Technical University of Munich Munich Germany The Department of Radiology San Francisco VA Medical Center San FranciscoCA United States

A major remaining challenge for magnetic resonance-based attenuation correction methods (MRAC) is their susceptibility to sources of MRI artifacts (e.g. implants, motion) and uncertainties due to the limitations of MRI contrast (e.g. accurate bone delineation and density, and separation of air/bone). We propose using a Bayesian deep convolutional neural network that, in addition to generating an initial pseudo-CT from MR data, also produces uncertainty estimates of the pseudo-CT to quantify the limitations of the MR data. These outputs are combined with MLAA reconstruction that uses the PET emission data to improve the attenuation maps. With the proposed approach (UpCT-MLAA), we demonstrate accurate estimation of PET uptake in pelvic lesions and show recovery of metal implants. In patients without implants, UpCT-MLAA had acceptable but slightly higher RMSE than Zero-echo-time and Dixon Deep pseudo-CT when compared to CTAC. In patients with metal implants, MLAA recovered the metal implant;however, anatomy outside the implant region was obscured by noise and crosstalk artifacts. Attenuation coefficients from the pseudo-CT from Dixon MRI were accurate in normal anatomy;however, the metal implant region was estimated to have attenuation coefficients of air. UpCT-MLAA estimated attenuation coefficients of metal implants alongside accurate anatomic depiction outside of implant regions. © 2020, CC BY.

关键词： Magnetic resonance

Phased Array Beamforming Methods for Powering Biomedical Ultrasonic Implants

学校读者我要写书评

暂无评论

arXiv 2022年

作者： Benedict, Braeden C. Ghanbari, Mohammad Meraj Muller, Rikky UC Berkeley UC San Francisco Joint Program in Bioengineering BerkeleyCA94720 United States The Department of Electrical Engineering and Computer Sciences University of California Berkeley BerkeleyCA94720 United States The Chan Zuckerberg Biohub San FranciscoCA94158 United States

Millimeter-scale implants using ultrasound for power and communication have been proposed for a range of deep-tissue applications, including neural recording and stimulation. However, published implementations have shown high sensitivity to misalignment with the external ultrasound transducer. Ultrasonic beamforming using a phased array to these implants can improve tolerance to misalignment, reduce implant volume, and allow multiple implants to be operated simultaneously in different locations. This paper details the design of a custom planar phased array ultrasound system, which is capable of steering and focusing ultrasound power within a 3D volume. Analysis and simulation is performed to determine the choice of array element pitch, with special attention given to maximizing the power available at the implant while meeting FDA limits for diagnostic ultrasound. Time reversal is proposed as a computationally simple approach to beamforming that is robust despite scattering and inhomogeneity of the acoustic medium. This technique is demonstrated both in active drive and pulse-echo modes, and it is experimentally compared with other beamforming techniques by measuring energy transfer efficiency. Simultaneous power delivery to multiple implants is also demonstrated. Copyright © 2022, The Authors. All rights reserved.

关键词： Beamforming

Direct Detection of Adenovirus in Environmental Waste Waters by Portable Optical Fiber Sensor Platform

学校读者我要写书评

暂无评论

Direct Detection of Adenovirus in Environmental Waste Waters...

IEEE Sensors

作者： Nimet Yildirim Dan Li Feng Long April Z. Gu Bioengineering PhD Program Northeastern University Boston US State Key Joint Laboratory of Environment Simulation and Pollution Control Environmental Science and Engineering Tsinghua University Beijing China Department of Environment Science and Engineering Tsinghua University Beijing China Department of Civil and Environmental Engineering Northeastern University Boston US

ISBN: (纸本)9781467346412

Reusable and portable evanescent wave biosensor for rapid, sensitive and highly selective direct detection of adenoviruses was developed. Here, specific antibody for the hexon protein of the virus was used as a recognition element. Linear detection range was determined from 100PFU/ml to 2000PFU/ml. This direct detection system can be regenerated successfully with over 5 times without significant deterioration of the sensor performance. The developed biosensor system has high specificity towards adenovirus, and it was applied to environmental waste water samples for the detection of adenovirus with 95 to 117% recovery and 1.1 to 8.1% cv values (n=2).

关键词： ADENOVIRUS DETECTION DIRECT

Hairpin RF Resonators for Transceiver Arrays with High Inter-channel Isolation and B1 Efficiency at Ultrahigh Field 7T MR Imaging

学校读者我要写书评

暂无评论

arXiv 2022年

作者： Payne, Komlan Ying, Leslie L. Zhang, Xiaoliang Department of Biomedical Engineering State University of New York at Buffalo BuffaloNY14260 United States Department of Electrical Engineering State University of New York at Buffalo BuffaloNY14260 United States Univerity of California Berkeley UCSF Joint Bioengineering Program San FranciscoCA94158 United States

Electromagnetic decoupling among a close-fitting or high-density transceiver RF array elements is required to maintain the integrity of the magnetic flux density from individual channel for enhanced performance in detection sensitivity and parallel imaging. High-impedance RF coils have demonstrated to be a prominent design method to circumvent these coupling issues. Yet, inherent characteristics of these coils have ramification on the B1 field efficiency and SNR. In this work, we propose a hairpin high impedance RF resonator design for highly decoupled multichannel transceiver arrays at ultrahigh magnetic fields. Due to the high impedance property of the hairpin resonators, the proposed transceiver array can provide high decoupling performance without using any dedicated decoupling circuit among the resonant elements. Because of elimination of lumped inductors in the resonator circuit, higher B1 field efficiency in imaging subjects can be expected. In order to validate the feasibility of the proposed hairpin RF coils, systematical studies on decoupling performance, field distribution, and SNR are performed, and the results are compared with those obtained from existing high-impedance RF coil, e.g., "self-decoupled RF coil". To further investigate its performance, an 8-channel head coil array using the proposed hairpin resonators loaded with a cylindrical phantom is designed, demonstrating a 19 % increase of the B1+ field intensity compared to the self-decoupled coils at 7T. Furthermore, the characteristics of the hairpin RF coils are evaluated using a more realistic human head voxel model numerically. The proposed hairpin RF coil provides excellent decoupling performance and superior RF magnetic field efficiency compared to the "self-decoupled" high impedance coils. Bench test of a pair of fabricated hairpin coils prove to be in good accordance with numerical results. © 2022, CC BY.

关键词： Magnetic resonance imaging

Dish-trend: Intervention modeling simulator that accounts for trend influences

学校读者我要写书评

暂无评论

arXiv 2021年

作者： Andjelkovic, Stefan Miskov-Zivanov, Natasa Computational and Systems Biology Joint Carnegie Mellon University University of Pittsburgh Computational Biology PhD Program University of Pittsburgh 4420 Bayard St PittsburghPA15213 United States Electrical and Computer Engineering Bioengineering Computational and Systems Biology University of Pittsburgh 4420 Bayard St PittsburghPA15213 United States

关键词： Stochastic systems