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10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

作者： Zhang, Mimi Y. Di Carlo, Dino Wu, Liz Y. Lee, Luke P. Berkeley Sensor and Actuator Center UCSF/UCB Joint Program in Bioengineering University of California Berkeley CA United States

To effectively coalesce otherwise monolayer liver cells (hepatocytes) seeded into microfluidic channels, we present a high-density cell-trapping array chip for rapid three-dimensional hepatocyte spheroid self-assembly. Moreover, by modifying trap geometries, spheroids of varying sizes can be formed in order to optimize the spatial microenvironment for improving cell viability and function. Preliminary sensitivity analysis of albumin secretion detection demonstrates the feasibility of using an optimized chemiluminescent immunoassay to determine the albumin concentrations in the range of 1 ng/mL to 500 ng/mL. © 2006 Society for Chemistry and Micro-Nano Systems.

关键词： Cell culture

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Miniscope3D:optimized single-shot miniature 3D fluorescence microscopy

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Light(Science & Applications) 2020年第1期9卷 367-379页

作者： Kyrollos Yanny Nick Antipa William Liberti Sam Dehaeck Kristina Monakhova Fanglin Linda Liu Konlin Shen Ren Ng Laura Waller UCB/UCSF Joint Graduate Program in Bioengineering University of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering&Computer Sciences University of CaliforniaBerkeleyCA 94720USA TIPs Department Universite libre de Bruxelles(ULB)1050 BrusselsBelgium

Miniature fluorescence microscopes are a standard tool in systems ***,widefield miniature microscopes capture only 2D information,and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth ***,we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture *** the phase mask at the aperture stop significantly reduces the size of the device,and varying the focal lengths enables a uniform resolution across a wide depth *** phase mask encodes the 3D fluorescence intensity into a single 2D measurement,and the 3D volume is recovered by solving a sparsity-constrained inverse *** provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the fieldvarying aberrations in miniature *** demonstrate a prototype that is 17mm tall and weighs 2.5 grams,achieving 2.76μm lateral,and 15μm axial resolution across most of the 900×700×390μm^(3) volume at 40 volumes per *** performance is validated experimentally on resolution targets,dynamic biological samples,and mouse brain *** with existing miniature single-shot volume-capture implementations,our system is smaller and lighter and achieves a more than 2×better lateral and axial resolution throughout a 10×larger usable depth *** microscope design provides single-shot 3D imaging for applications where a compact platform matters,such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

关键词： enable resolution narrow

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MultiWienerNet: Deep learning for fast shift-varying deconvolution

MultiWienerNet: Deep learning for fast shift-varying deconvo...

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Computational Optical Sensing and Imaging, COSI 2021 - Part of OSA Imaging and Applied Optics Congress 2021

作者： Shuai, Richard W. Yanny, Kyrollos Monakhova, Kristina Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States

We present a deep-learning method based on Wiener filters and U-Nets that performs image reconstruction in systems with spatially-varying aberrations. We train on simulated microscopy measurements and test on experime... 详细信息

ISBN: (纸本)9781557528209

关键词： Image reconstruction

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Information-Theoretic Design for High-Dimensional Computational Imaging

Information-Theoretic Design for High-Dimensional Computatio...

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Computational Optical Sensing and Imaging, COSI 2024 - Part of Optica Imaging Congress

作者： Markley, Eric Kabuli, Leyla Chien, Tiffany Pinkard, Henry Waller, Laura UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States Department of Electrical Engineering and Computer Sciences University of California Berkeley United States

We present a technique for information-theoretic optimization of computational imaging systems demonstrated in snapshot 3D microscopy. By directly evaluating measurement quality and decoupling optimization from downst... 详细信息

关键词： Decoding

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Spectral DefocusCam: hyperspectral imaging using defocus and a spectral filter array

Spectral DefocusCam: hyperspectral imaging using defocus and...

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Computational Optical Sensing and Imaging, COSI 2022

作者： Foley, Christian Monakhova, Kristina Yanny, Kyrollos Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States

We propose a compact hyperspectral camera based on a focus-tunable lens and spectral filter array. The camera captures then fuses multiple defocused measurements to obtain a high-resolution hyperspectral volume. ©... 详细信息

ISBN: (纸本)9781557528209

关键词： Hyperspectral imaging

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Snapshot hyperspectral imaging using a random phase mask and spectral filter array

Snapshot hyperspectral imaging using a random phase mask and...

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Computational Optical Sensing and Imaging, COSI 2020 - Part of Imaging and Applied Optics Congress 2020

作者： Monakhova, Kristina Yanny, Kyrollos Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States

We introduce a snapshot hyperspectral imager that uses a random phase mask, repeated spectral filter array, and compressive recovery to achieve high spatial and spectral resolution in a small form factor. © 2020 ... 详细信息

ISBN: (纸本)9781557528209

关键词： Hyperspectral imaging

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Compressed sensing 3D fluorescence microscopy using optimized phase mask

Compressed sensing 3D fluorescence microscopy using optimize...

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Computational Optical Sensing and Imaging, COSI 2020 - Part of Imaging and Applied Optics Congress 2020

作者： Yanny, Kyrollos Antipa, Nick Liberti, William Dehaeck, Sam Monakhova, Kristina Liu, Fanglin Linda Shen, Konlin Ng, Ren Waller, Laura UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States Department of Electrical Engineering and Computer Sciences University of California Berkeley United States Brussels1050 Belgium

We demonstrate a single-shot miniature 3D computational microscope with an optimized phase encoder. Our method uses sparsity-based reconstruction to achieve a 2.76µm lateral and 15µm axial resolution across ... 详细信息

ISBN: (纸本)9781557528209

关键词： Fluorescence microscopy

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The multiscale in silico liver

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Journal of Critical Care 2007年第4期22卷 348-349页

作者： Hunt, C. Anthony Yan, Li Ropella, Glen E.P. Park, Sunwoo Sheikh-Bahaei, Shahab Roberts, Michael S. University of California San Francisco USA Joint UCSF/UCB Bioengineering Program University of Queensland Australia

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The utilization of a multimedia PACS workstation for surgical planning of epilepsy

The utilization of a multimedia PACS workstation for surgica...

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Medical Imaging 1997: PACS Design and Evaluation: Engineering and Clinical Issues

作者： Hoo, Kent Soo Wong, Stephen T.C. Hawkins, Randall A. Knowlton, Robert Laxer, Kenneth D. Rowley, Howard Joint Program in Bioengineering UCSF UCB CA94143 United States Philips Research Palo AltoCA94304 United States Department of Radiology UCSF United States Department of Neurology UCSF United States Swedish Medical Center SeattleWA98114 United States

Surgical treatment of temporal lobe epilepsy (TLE) requires the localization of the epileptogenic zone for surgical resection. Currently, clinicians utilize electroencephalography (BEG), various neuroimaging modalities, and psychological tests together to determine the location of this zone. We investigate how a multimedia neuroimaging workstation built on top of the ucsf Picture Archiving and Communication System (PACS) can be used to aid surgical planning of epilepsy and related brain diseases. This usage demonstrates the ability of the workstation to retrieve image and textual data from PACS and other image sources, register multimodality images, visualize and render 3D data sets, analyze images, generate new image and text data from the analysis, and organize all data in a relational database management system. © 1997 SPIE.

关键词： Medical applications

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Spatially-varying microscope calibration from unstructured sparse inputs

Spatially-varying microscope calibration from unstructured s...

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Computational Optical Sensing and Imaging, COSI 2020 - Part of Imaging and Applied Optics Congress 2020

作者： Kuo, Grace Monakhova, Kristina Yanny, Kyrollos Ng, Ren Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley BerkeleyCA94720 United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley BerkeleyCA94720 United States

We propose a method based on blind deconvolution to calibrate the spatially-varying point spread functions of a coded-aperture microscope system. From easy-to-acquire measurements of unstructured fluorescent beads, we... 详细信息

ISBN: (纸本)9781557528209

关键词： Optical transfer function

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