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Spectral DiffuserScope - Computational Snapshot Hyperspectral Imager for Microscopy

Spectral DiffuserScope - Computational Snapshot Hyperspectra...

2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023

作者： Aggarwal, Neerja Markley, Eric Raniwala, Yashovardhan Houck, William D. Lee, Kyung Chul Bae, Junghyun Baek, Nakkyu Park, Wook Lee, Seung Ah Waller, Laura Department of Electrical Engineering and Computer Sciences University of California Berkeley United States UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States VIAVI Solutions Inc Santa Rosa United States School of Electrical and Electronic Engineering Yonsei University Seoul Korea Republic of

We introduce a computational snapshot hyperspectral imager for benchtop microscopy. The compact device uses a diffuser and spectral filter array to multiplex spatio-spectral information into a 2D measurement, then rec... 详细信息

ISBN: (纸本)9781957171289

关键词： Blue light emitting diodes Diffusers Fluorescence microscopy Image sensors Spatial resolution Ultraviolet light emitting diodes

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Physics-based learned design for Fourier DiffuserScope

Physics-based learned design for Fourier DiffuserScope

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Computational Optical Sensing and Imaging, COSI 2021 - Part of OSA Imaging and Applied Optics Congress 2021

作者： Markley, Eric Liu, Fanglin Linda Kellman, Michael Antipa, Nick Waller, Laura UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley United States Department of Electrical Engineering and Computer Sciences University of California Berkeley United States Department of Pharmaceutical Chemistry University of California San Francisco United States Department of Electrical and Computer Engineering University of California San Diego United States

We couple a differentiable forward model of diffuser-based single-shot 3D microscopy (Fourier DiffuserScope) with an invertible and differentiable reconstruction algorithm to jointly optimize the imaging system and th... 详细信息

ISBN: (纸本)9781557528209

关键词： Imaging systems

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Fourier diffuserscope: Single-shot 3d fourier light field microscopy with a diffuser

arXiv

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arXiv 2020年

作者： Liu, Fanglin Linda Grace, K.U.O. Antipa, Nick Yanny, Kyrollos Waller, Laura Electrical Engineering and Computer Sciences University of California Berkeley94709 United States UCB/UCSF Joint Graduate Program in Bioengineering University of California BerkeleyCA94709 United States

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample, without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of lateral resolution at the focal plane, which is highly undesirable in microscopy. Placing the MLA near the pupil plane of the microscope, rather than the image plane, can mitigate the artifacts near focus and provide an efficient shift-invariant model at the expense of field-of-view. Here, we show that our Fourier DiffuserScope achieves significantly better performance than Fourier LFM. Fourier DiffuserScope uses a diffuser in the pupil plane to encode depth information, then reconstructs volumetric information computationally by solving a sparsity-constrained inverse problem. Our diffuser consists of randomly placed microlenses with varying focal lengths. We show that by randomizing the microlens positions, a larger lateral field-of-view can be achieved compared to a conventional MLA;furthermore, by adding diversity to the focal lengths, the axial depth range is increased. To predict system performance based on diffuser parameters, we for the first time establish a theoretical framework as a design guideline, followed by numerical simulations to verify. Under both theoretical and numerical analysis, we demonstrate that our diffuser design provides more uniform resolution over a larger volume, both laterally and axially, outperforming the MLA used in LFM. We build an experiment system with an optimized lens set and achieve 3 volume. Copyright © 2020, The Authors. All rights reserved.

关键词： Microlenses

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Spectral DiffuserCam: lensless snapshot hyperspectral imaging with a spectral filter array

arXiv

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arXiv 2020年

作者： Monakhova, Kristina Yanny, Kyrollos Aggarwal, Neerja Waller, Laura Department of Electrical Engineering & Computer Sciences University of California BerkeleyCA94720 United States UCB/UCSF Joint Graduate Program in Bioengineering University of California BerkeleyCA94720 United States

Hyperspectral imaging is useful for applications ranging from medical diagnostics to crop monitoring;however, traditional scanning hyperspectral imagers are prohibitively slow and expensive for widespread adoption. Snapshot techniques exist but are often confined to bulky bench-top setups or have low spatio-spectral resolution. In this paper, we propose a novel, compact, and inexpensive computational camera for snapshot hyperspectral imaging. Our system consists of a repeated spectral filter array placed directly on the image sensor and a diffuser placed close to the sensor. Each point in the world maps to a unique pseudorandom pattern on the spectral filter array, which encodes multiplexed spatio-spectral information. A sparsity-constrained inverse problem solver then recovers the hyperspectral volume with good spatio-spectral resolution. By using a spectral filter array, our hyperspectral imaging framework is flexible and can be designed with contiguous or non-contiguous spectral filters that can be chosen for a given application. We provide theory for system design, demonstrate a prototype device, and present experimental results with high spatio-spectral resolution. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement Copyright © 2020, The Authors. All rights reserved.

关键词： Spectroscopy

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Author Correction: Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

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Light, science & applications 2023年第1期12卷 93页

作者： Kyrollos Yanny Nick Antipa William Liberti Sam Dehaeck Kristina Monakhova Fanglin Linda Liu Konlin Shen Ren Ng Laura Waller UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley CA 94720 USA. kyrollosyanny@***. Department of Electrical Engineering & Computer Sciences University of California Berkeley CA 94720 USA. naantipa@***. Department of Electrical Engineering & Computer Sciences University of California Berkeley CA 94720 USA. TIPs Department Université libre de Bruxelles (ULB) 1050 Brussels Belgium. UCB/UCSF Joint Graduate Program in Bioengineering University of California Berkeley CA 94720 USA.

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