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作者： Liu,X. Dang,Y. Sun,D. Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science & Engineering Beijing Forestry University 35 Tsinghua East Road Beijing 100083 China

Anaerobically treated leachate from municipal solid waste incineration plants contains extremely high ammonia concentrations（1200～1600 mg/L NH4+-N）. The nitrogen removal process treated by short-cut nitrification/denitrification produced a high level free nitrous acid（FNA）which can hinder short-cut nitrification processes. In order to develop strategies that will help overcome this limitation, thresholds of FNA inhibition on ammonia oxidation（nitritation） were identified in laboratory-scale sequencing batch reactors, which was ～0.1 mg/L for nitritation. pH adjustments made during the nitritation process kept FNA concentrations below 0.1 mg/L, enabling reactors to operate when total ammonia nitrogen（TAN） concentrations were 1400 mg/Lwith 1000～1200 mg/L nitrite produced at the end of nitritation. Reactors without p H adjustment, on the other hand, deteriorated after a period of *** ammonia oxidizing bacteria were predominant members（70%） of the microbial community found in the pH-adjusted reactor. The majority of metabolically active heterotrophic nitrifiers clustered with the genus Paracoccus（～90%）, while all of the autotrophic nitrifiers were Nitrosomonas *** reverse transcription（qRT） PCR was done on RNA extracted from the pH-adjusted reactor operated at TAN concentrations of 1400 mg/L and exposed to various concentrations of FNA（0.018, 0.103, 0.195, and 0.328 mg/L）.The results showed that transcription of heterotrophicamoA genes was significantly inhibited by elevated FNA concentrations, while the number of autotrophic amoA mRNA transcripts was positively correlated with FNA concentrations.

关键词： AmoA gene transcript Autotrophic nitrifier FNA inhibition threshold Heterotrophic nitrifier Incineration leachate Short-cut nitrification

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Phosphine production in anaerobic wastewater treatment under tetracycline antibiotic pressure

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Journal of Environmental Sciences 2018年第7期30卷 239-250页

作者： Meiqing Lu Xiaojun Niu Weiyi Chen Zhu Liang Sheng You Xiaohong Gu School of Environment and Energy South China University of Technology Guangzhou 510006 China State Key Laboratory of Lake Science and Environment Nanjing 210008 China School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen Key Laboratory of Soil and Groundwater Pollution Control Shenzhen 518055 China Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control Guangzhou 510640 China China Water Resources Pearl River Planning Surveying & Designing Co. Ltd. Guangzhou 510640 China The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of EducationSouth China University of Technology Guangzhou Higher Education Mega Centre Guangzhou 510006 China

The influence of tetracycline（TC） antibiotics on phosphine（PH3） production in the anaerobic wastewater treatment was studied. A lab-scale anaerobic baffled reactor with three compartments was employed to simulate this process. The reactor was operated in a TC-absence wastewater and 250 μg/L TC-presence wastewater for three months after a start-up period,respectively. The responses of p H, oxidation–reduction potential（ORP）, chemical oxygen demand（COD）, total phosphorus（TP）, enzymes activity（dehydrogenase and acid phosphatase）,and microbial community were investigated to reveal the effect of TC on PH3 *** suggested that the dehydrogenase（DH） activity, acid phosphatase（ACP） activity and COD have positive relationship with PH3 production, while p H, ORP level and the TP in liquid phase have negative relationship with PH3 production. With prolonged TC exposure, decrease in p H and increase in DH activity are beneficial to PH3 production, while decrease in COD and ACP activity are not the limiting factors for PH3 production.

关键词： Tetracycline antibiotic Anaerobic Wastewater treatment Phosphine production

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Iron-doped biochar boosting salt marsh blue carbon via regulation of microbial metabolism and intensified mineral-associated organic carbon

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Journal of Cleaner Production 2025年 514卷

作者： Su, Guojiang Huang, Yuzhou Fan, Dongyang Pan, Yaran Zhu, Yingjie Liu, Yang Huang, Haomin Xiao, Xi Ocean College Zhejiang University 1 Zheda Road Zhejiang Zhoushan316021 China Key Laboratory of Tropical Marine Ecosystem and Bioresource Fourth Institute of Oceanography Ministry of Natural Resources Beihai536000 China Guangxi Key Laboratory of Beibu Gulf Marine Resources Environment and Sustainable Development Fourth Institute of Oceanography Ministry of Natural Resources Beihai536000 China College of Agriculture and Biotechnology Zhejiang University Hangzhou China School of Environment and Energy South China University of Technology Guangzhou510006 China Joint Center for Blue Carbon Research Ocean Academy Zhejiang University Zheda Road Zhejiang Zhoushan316000 China Donghai Laboratory Zhejiang Zhoushan316021 China Key Laboratory of Watershed Non-point Source Pollution Control and Water Eco-security of Ministry of Water Resources College of Environmental and Resources Sciences Zhejiang University Hangzhou310058 China

Salt marsh ecosystems, with substantial carbon sequestration capacity and ecological value, are experiencing a persistent decline globally. Biochar, especially that derived from Spartina alterniflora, is a promising material for restoration, but conventional biochar has shown limited effectiveness. This study introduces iron-doped biochar (FSBC) as an innovative material that boosts salt marsh blue carbon via regulation of microbial metabolism and intensified mineral-associated organic carbon (MAOC) formation. In experimental cultivation of Sesuvium portulacastrum, the addition of FSBC increased biomass density by 332.4 %, carbon stock by 14.0 %, and MAOC by 38.3 % compared to biochar without iron amendment. These results suggest that FSBC can significantly improve both plant growth and carbon storage in salt marshes. Through microbiome and metabolome analyses, we elucidated the regulatory mechanisms of Fe in plants and its protective role in soil carbon components. FSBC affected bacteria associated with Fe like Pseudomonadales and Croceicoccus, thereby influencing metabolic pathways related to energy transporters and nutrient uptake in plants, including ABC transporters and phenylalanine metabolism. These changes in metabolic pathways promote the accumulation of soil organic acids, enhancing plant iron nutrition. Additionally, FSBC regulates soil Fe components and key microbial communities, promoting Fe-carbon complexation and reducing carbon-related extracellular enzyme activity, thereby enhancing stable carbon accumulation. In conclusion, regulating microbial metabolism and intensifying mineral-associated organic carbon by addition of iron-doped biochar significantly enhance blue carbon sequestration in salt marshes. This provides a promising approach for salt marsh restoration. © 2025 Elsevier Ltd

关键词： Listeria

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Binding of per- and polyfluoroalkyl substances with liver and serum proteins in rats: implications for physiologically based pharmacokinetic modelling

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Environment International 2025年 201卷

作者： Xiarui Fan Xiaomin Li Tong Li Bing Shao Shan Niu Wenhong Fan Qi Wang Lv Zhong Xiangrui Wang Ziwei Wang Fujun Ma Guomao Zheng Hui Peng Lili Chen Zhaomin Dong School of Materials Science and Engineering Beihang University Beijing 100191 China Institute of Quality Standards and Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences Beijing 100081 China Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety College of Veterinary Medicine China Agricultural University Beijing 100193 China Advanced Interdisciplinary Institute of Environment and Ecology Guangdong Provincial Key Laboratory of Wastewater Information Analysis and Early Warning Beijing Normal University Zhuhai 519087 China Beijing Key Lab for Source Control Technology of Water Pollution College of Environmental Sciences & Engineering Beijing Forestry University Beijing 100083 China Southern Univ Sci & Technol Sch Environm Sci & Engn Shenzhen 518055 China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences Beijing 100012 China Department of Chemistry University of Toronto Toronto ON M5S 3H6 Canada School of the Environment University of Toronto Toronto ON M5S 3H6 Canada Structural Genomics Consortium Toronto ON M5S 3H6 Canada School of Public Health Southeast University Nanjing China

Understanding the binding between per- and polyfluoroalkyl substances (PFAS) and proteins is essential for elucidating their toxicokinetics and tissue distribution. Here, we quantified the binding affinities of 14 PFAS to rat liver fatty acid-binding protein (rL-FABP) and rat serum albumin (RSA). Results showed that PFAS exhibit strong binding affinities (K a ) to the rL-FABP (10 3 ∼ 10 5 M −1 ), particularly among medium- to long-chain perfluorinated carboxylic acids (PFCAs). The binding affinity of PFAS to RSA ranged from approximately 10 4 to 10 5 M −1 , with 1 to 4 binding sites. Molecular docking results supported that PFAS binding to proteins is an exothermic process driven by van der Waals forces, hydrogen bonding, and electrostatic interactions. Additionally, long-chain PFCAs were shown to adopt a “U”-shaped conformation within the ligand-binding cavities of rL-FABP and RSA. The newly developed physiologically based pharmacokinetic model using measured binding data demonstrates a substantial improvement in the goodness of fit to experimental observations, reducing the prediction error by 20 %∼216 %. Finally, we found that the PFAS liver-blood partition could be mainly explained by the binding affinity ratios of PFAS to liver and blood proteins, which could be further extrapolated from rats to humans, providing useful insights to understand the tissue distribution of PFAS.

关键词： PFAS Protein binding PBPK model Liver-blood partition Toxicokinetics

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Potential acute effects of suspended aluminum nitride (AlN) nanoparticles on soluble microbial products (SMP) of activated sludge

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Journal of Environmental Sciences 2017年第7期29卷 284-292页

作者： Lijie Zhou Weiqin Zhuang Xin Wang Ke Yu Shufang Yang Siqing Xia State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology Shenzhen Key Laboratory of Drinking Water Source Safety ControlShenzhen Key Laboratory of Emerging Contaminants Detection & Control in Water EnvironmentShenzhen Academy of Environmental Sciences State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji University Department of Civil and Environmental Engineering University of Auckland School of Environment and Energy Peking University Shenzhen Graduate School Shenzhen Municipal Design & Research Institute Co. Ltd

The study aims to identify the potential acute effects of suspended aluminum nitride（Al N）nanoparticles（NPs） on soluble microbial products（SMP） of activated *** activated sludge loaded with 1,10,50,100,150 and 200 mg/L of Al N NPs were carried out in this *** results showed,Al N NPs had a highly inverse proportionality to bacterial dehydrogenase and OUR,indicating its direct toxicity to the activated sludge *** toxicity of Al N NPs was mainly due to the nano-scale of Al N *** SMP,Al N NPs led to the decrease of polysaccharide and humic compounds,but had slight effects on *** decrease of tryptophan-like substances in SMP indicated the inhibition of Al N NPs on the bacterial ***,Al N NPs reduced obviously the molecular weight of SMP,which might be due to the nano-scale of Al N.

关键词： Aluminum nitride nanoparticles Topic Soluble microbial products（SMP） Activated sludge Bacterial viability

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Evaluation of soil erosion risks in a coal mine based on an integrated extension-cloud model

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Journal of Mines, Metals and Fuels 2018年第1期66卷 8-12页

作者： Li, Li Yue, Qiang Shaohong, Li Civil Engineering College Chongqing Three Gorges University Chongqing404 100 China Changjiang River Scientific Research Institute of Changjiang Water Resources Commission Wuhan430 010 China Key Lab. of Three Gorges Reservoir Area Environ. Change and Pollution Control of Water Environment Chongqing Three Gorges University Chongqing404 100 China College of Environment and Civil Engineering Chengdu University of Technology Chengdu610 059 China

Soil and water losses have become a serious ecological and environmental problems, and how to scientifically carry out soil and water losses risk assessment on soil erosion prevention and treatment of macroeconomic decisionmaking are very important. Based on the concept of cloud matter element, the associated function and cloud model were used to calculate the association value, then an improved matter-element model of extension cloud combination was established, at last, the evaluation model was introduced into soil and water losses risk assessment. Through the project case in a coal mine shows that the improved evaluation model can get a reasonable risk evaluation result. In practical application, the evaluation result of the extension cloud combination model constructed by classical extension correlation function is closer to the visual statistical value. © 2018 Books and Journals Private Ltd. All rights reserved.

关键词： Coal mines

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Cationic Polymeric Networks: 3D Cationic Polymeric Network Nanotrap for Efficient Collection of Perrhenate Anion from Wastewater (Small 20/2021)

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Small 2021年第20期17卷

作者： Xiaorui Li Yiming Li Huifang Wang Zheng Niu Yingjie He Linfeng Jin Mingyang Wu Haiying Wang Liyuan Chai Abdullah M. Al-Enizi Ayman Nafady Shoyebmohamad F. Shaikh Shengqian Ma School of Metallurgy and Environment Central South University Changsha Hunan 410083 China Department of Chemistry University of South Florida Tampa FL 33620 USA College of Chemistry and Chemical Engineering Central South University Changsha Hunan 410083 China College of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou Jiangsu 215123 China Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution Changsha Hunan 410083 China Water Pollution Control Technology Key Lab of Hunan Province Changsha 410004 China Department of Chemistry Collage of Science King Saud University Riyadh 11451 Saudi Arabia Department of Chemistry University of North Texas Denton TX 76201 USA

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Reversibility of the structure and dewaterability of anaerobic digested sludge

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Journal of Environmental Sciences 2016年第4期28卷 178-186页

作者： Yiqi Sheng Yili Wang Wei Hu Xu Qian Huaili Zheng Xiaoxiu Lun College of Environmental Science and Engineering Beijing Key Lab for Source Control Technology of Water PollutionBeijing Forestry University College of Urban Construction and Environmental Engineering Chongqing University

The reversibility of the structure and dewaterability of broken anaerobic digested sludge（ADS）is important to ensure the efficiency of sludge treatment or management *** study investigated the effect of continuous strong shear（CSS）and multipulse shear（MPS）on the zeta potential,size（median size,d（50））,mass fractal dimension（DF）,and capillary suction time（CST）of ADS ***,the self-regrowth（SR）of broken ADS aggregates during slow mixing was also *** results show that raw ADS with d（50） of 56.5μm was insensitive to CSS–SR or MPS–SR,though the size slightly decreased after the breakage *** conditioned ADS with d（50） larger than 600μm,the breakage in small-scale surface erosion changed to large-scale fragmentation as the CSS strength *** most cases,after CSS or MPS,the broken ADS had a relatively more compact structure than before and d（50） is at least 200μ*** CST of the broken fragments from optimally dosed ADS increased,whereas that corresponding to overdosed ADS *** treatment resulted in larger and more compact broken ADS fragments with a lower CST value than *** the subsequent slow mixing,the broken ADS aggregates did not recover their charge,size,and dewaterability to the initial values before *** addition,less than 15%self-regrowth in terms of percentage of the regrowth factor was observed in broken ADS after CSS at average velocity gradient no less than 1905.6 sec^（-1）.

关键词： Anaerobic digested sludge Shear Reversibility Structure Dewaterability Floc strength

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Selection and characterization of eight freshwater green algae strains for synchronous water purification and lipid production

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Frontiers of Environmental Science & Engineering 2016年第3期10卷 548-558页

作者： Jingjing ZHAN Qiao ZHANG Momei QIN Yu HONG Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science and Engineering Beijing Forestry University Beijing 100083 China State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology Shenzhen Key Laboratory of Drinking Water Source Safety Control Shenzhen Research Academy of Environmental Sciences Shenzhen 518001 China

The objective of this study is to select and characterize the candidate for synchronous water purification and lipid production from eight freshwater microalgae strains （Chlorella sp. HQ, C. emersonii, C. pyrenoidosa, C. vulgaris, Scenedesmus dimorphus, S. quadricauda, S. obiquus, Scenedesmus sp. LX1）. The strains Chlorella sp. HQ, C. pyrenoidesa, and S. obliquus showed superiority in biomass accumulation, while the top biomass producers did not correspond to the top lipid producers. S. quadricauda achieved higher lipid content （66.1%）, and Chlorella sp. HQ and S. dimorphus ranked down in sequence, with lipid content above 30%. Considering nutrient removal ability （total nitrogen （TN）： 52.97%; total phosphorus （TP）： 84.81%）, the newly isolated microalga Chlorella sp. HQ was the possible candidate for water purification coupled with lipid production. To further investigate the lipid producing and nutrient removal mechanism of candidate microalga, the ultra structural changes especially the lipid droplets under different water qualities （different TN and TP concentrations） were characterized. The results elucidate the nutrient-deficiency （TN： 3.0 mg·L^-1; TP： 0.3 mg·L^-1） condition was in favor of forming lipid bodies in Chlorella sp. HQ at the sub-cellular level, while the biomass production was inhibited due to the decrease in chloroplast number which could further suppress the nutrient removal effect. Finally, a two- phase cultivation process （a nutrient replete phase to produce biomass followed by a nutrient deplete phase to enhance lipid content） was conducted in a photo-bioreactor for Chlorella sp. HQ to serve for algae-based synchronous biodiesel production and wastewater purification.

关键词： freshwater microalgae biomass production lipid accumulation nutrient removal

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Effect of nitrogen/phosphorus concentration on algal organic matter generation of the diatom Nitzschia palea:Total indicators and spectroscopic characterization

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Journal of Environmental Sciences 2016年第9期28卷 130-142页

作者： Linlin Han Bingbing Xu Fei Qi Zhonglin Chen Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry University State Key Laboratory of the Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

Critical algal blooms in great lakes increase the level of algal organic matters（AOMs）,significantly altering the composition of natural organic matters（NOMs） in freshwater of *** study examined the AOM＇s characteristics of Nitzschia palea（***）,one kind of the predominant diatom and an important biomarker of water quality in the great lakes of China,to investigate the effect of AOMs on the variation of NOMs in lakes and the process of algal ***–emission matrix fluorescence（EEM） spectroscopy,synchronous fluorescence（SF） spectroscopy and deconvolution UV–vis（D-UV） spectroscopy were utilized to characterize AOMs to study the effects of nutrient loading on the composition change of *** results,it was revealed that the phosphorus is the limiting factor for ***＇s growth and the generation of both total organic carbon and amino acids but the nitrogen is more important for the generation of carbohydrates and *** spectra revealed differences in the composition of extracellular organic matter and intracellular organic *** of the nitrogen and phosphorus concentrations,aromatic proteins and soluble microbial products were the main components,but the nitrogen concentration had a significant impact on their *** SF spectra were used to study the AOMs for the first time and identified that the protein-like substances were the major component of AOMs,creating as a result of aromatic group *** D-UV spectra showed carboxylic acid and esters were the main functional groups in the EOMs,with –OCH_3,–SO_2NH_2,–CN,–NH_2,–O– and –COCH_3functional groups substituting into benzene rings.

关键词： Extracellular organic matter Intracellular organic matter Nitzschia palea Fluorescence spectroscopy Deconvolution UV–vis spectroscopy

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