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A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling

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Chinese Chemical Letters 2022年第6期33卷 3091-3096页

作者： Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China The Key Laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China

The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular *** reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical *** this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination *** on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable ***,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation *** maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple *** show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La ***,statistical analysis was performed for the mean peak height and mean peak area of the cellular *** believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications.

关键词： Microfluidics Dynamic stimulation Time code High-throughput Single-cell array

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A protocol for structured illumination microscopy with minimal reconstruction artifacts

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Biophysics Reports 2019年第2期5卷 80-90页

作者： Junchao Fan Xiaoshuai Huang Liuju Li Shan Tan Liangyi Chen Key Laboratory of Image Processing and Intelligent Control of Ministry of Education of China School of Automation Huazhong University of Science and Technology State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular Medicine Peking University

The imaging rate of structured illumination microscopy(SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a result, the signal-to-noise ratio(SNR) decreases sharply. Furthermore, the SNR decreases further because of photobleaching and phototoxicity. This decreased quality of SIM raw data may lead to surprising artifacts with various causes, which may confuse a new user of SIM microscopy. We summarize three significant possible sources of severe artifacts in reconstructed super-resolution(SR)images. Ultrafast motion of a biological sample or an uneven illumination pattern is the most difficult to be identified. The estimated parameter could also be incorrect, leading to artifact of regular ***, reconstruction with the Wiener method generates stochastic artifacts due to the amplification of noise during the deconvolution process. To deal with these problems, we have established a protocol to reconstruct ultrafast SIM raw data obtained in low SNR conditions. First, we checked the quality of the raw data with the image J plugin SIMcheck before reconstruction. Then, a modified parameter estimation method was used to improve the precision of the parameters. Finally, an iterative algorithm was used for SIM reconstruction under low signal-to-noise ratio conditions. This procedure effectively suppressed the artifacts in the super-resolution images reconstructed from raw data of low signal-to-noise ratio.

关键词： Ultrafast SIM Low signal-to-noise ratio Reconstruction

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Two novel cellular neural networks based on mem-elements 34

Two novel cellular neural networks based on mem-elements

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34th Chinese control Conference, CCC 2015

作者： Shen, Yi Zhenzhen, Jia Xiaoping, Wang Yang, Liu School of Automation Huazhong University of Science and Technology Wuhan China Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Wuhan China Research Institute of Huazhong University of Science and Technology in Shenzhen Shenzhen China

ISBN: (纸本)9789881563897

Two types of novel cellular neural networks based on mem-elements are proposed, namely, MC-CNN and EM-CNN. The MC-CNN lets a memcapacitor replace the conventional linear capacitor of a cellular neural network cell. This improvement takes advantage of the nanoscale of memcapacitor and its natural nonlinearity, which makes the MC-CNN more compact and the output function simplified. Mathematical analysis of stability and simulation of image processing is presented to verify the feasibility and performance of MC-CNN. The EM-CNN is an economical improvement of the memristor synapse cellular neural network. In the EM-CNN, based on the symmetry of CNN templates, the amount of memristors and voltage-controlled current source is largely reduced. Thus, the EM-CNN is not only economical on the fabricating cost of CNN but also possesses a simpler cell structure which is beneficial to better implementation of CNN. © 2015 Technical Committee on control Theory, Chinese Association of Automation.

关键词： Cellular Neural Network image processing Memcapacitor Memristor

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3D reconstruction of the coronary tree from two X-ray angiographic views

3D reconstruction of the coronary tree from two X-ray angiog...

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Medical Imaging 2006: image processing

作者： Sang, Nong Peng, Weixue Li, Heng Zhang, Zhen Zhang, Tianxu Institute for Pattern Recognition and Artificial Intelligence Huazhong University of Science and Technology Wuhan 430074 China Key Laboratory Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan 430074 China

ISBN: (纸本)0819464236

In this paper, we develop a method for the reconstruction of 3D coronary artery based on two perspective projections acquired on a standard single plane angiographic system in the same systole. Our reconstruction is based on the model of generalized cylinders, which are generated by sweeping a two-dimensional cross section along an axis in three-dimensional space. We restrict the cross section to be circular and always perpendicular to the tangent of the axis. Firstly, the vascular centerlines of the X-ray angiography images on both projections are semiautomatically extracted by multiscale vessel tracking using Gabor filters, and the radius of the coronary are also acquired simultaneously. Secondly, the relative geometry of the two projections is determined by the gantry information and 2D matching is realized through the epipolar geometry and the consistency of the vessels. Thirdly, we determine the three-dimensional (3D) coordinates of the identified object points from the image coordinates of the matched points and the calculated imaging system geometry. Finally, we link the consequent cross sections which are processed according to the radius and the direction information to obtain the 3D structure of the artery. The proposed 3D reconstruction method is validated on real data and is shown to perform robustly and accurately in the presence of noise.

关键词： image reconstruction

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Quantitative study on despeckle methods of medical ultrasound images

Quantitative study on despeckle methods of medical ultrasoun...

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2011 IEEE International conference on intelligent Computation and Bio-Medical Instrumentation, ICBMI 2011

作者： Ma, Runxia Zhang, Xuming Ding, Mingyue Liu, Qi Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China Life College of Science and Technology Huazhong University of Science and Technology Wuhan China School of Electrical Information Engineering Sichuan University Chengdu China

ISBN: (纸本)9780769546230

Speckle is a granular noise that inherently exists in all types of coherent imaging systems. This paper presents a quantitative study on five despeckling methods such as frost filter, kuan filter, speckle reducing an isotropic diffusion, homomorphic filter and wavelet filter. We select six objective evaluation parameters, such as signal-to-ratio, contrast signal-to-noise ratio, figure of merit, least absolute error, edge protection factor, signal-to-minimum mean square error ratio, to quantify the performance of these filters. The comparative study will provide some parameter comparative reference for selecting a suitable filter in the ultrasound image processing. © 2011 IEEE.

关键词： Mean square error

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Adaptive thresholding of digital subtraction angiography images

Adaptive thresholding of digital subtraction angiography ima...

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MIPPR 2005: image Analysis Techniques

作者： Sang, Nong Li, Heng Peng, Weixue Zhang, Tianxu Institute for Pattern Recognition and Artificial Intelligence Huazhong University of Science and Technology Wuhan 430074 China Key Laboratory Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan 430074 China

In clinical practice, digital subtraction angiography (DSA) is a powerful technique for the visualization of blood vessels in the human body. Blood vessel segmentation is a main problem for 3D vascular reconstruction. In this paper, we propose a new adaptive thresholding method for the segmentation of DSA images. Each pixel of the DSA images is declared to be a vessel/background point with regard to a threshold and a few local characteristic limits depending on some information contained in the pixel neighborhood window. The size of the neighborhood window is set according to a priori knowledge of the diameter of vessels to make sure that each window contains the background definitely. Some experiments on cerebral DSA images are given, which show that our proposed method yields better results than global thresholding methods and some other local thresholding methods do.

关键词： image analysis

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A research of heart rate prediction model based on evolutionary neural network

A research of heart rate prediction model based on evolution...

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2011 IEEE International conference on intelligent Computation and Bio-Medical Instrumentation, ICBMI 2011

作者： Xiao, Feng Yuchi, Ming Ding, Mingyue Jo, Jun Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China Life College of Science and Technology Huazhong University of Science and Technology Wuhan China School of Information and Communication Technology Griffith University QLD Australia

ISBN: (纸本)9780769546230

Heart rate (HR) signal analysis is widely used in the medicine and medical research area. Physical activities (PA) are commonly recognized to greatly affect the changes of heart rate. A method of Evolutionary Neural Network - Neuro-evolution of Augmenting Topologies (NEAT) is used to build a PA-based HR predictor model. Through special coding, crossover and mutation operator, NEAT can implement network topology and connectivity weights evolution simultaneously. The common problem in evolutionary neural network, like competing conventions, how to protect the new innovation are effectively solved. The experimental results demonstrated the application potential of the approach. © 2011 IEEE.

关键词： Neural networks

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Efficient parallel architecture for lifting-based two-dimensional discrete wavelet transform

Efficient parallel architecture for lifting-based two-dimens...

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2005 IEEE International Workshop on VLSI Design and Video Technology, IWVDVT 2005

作者： Xiong, Cheng-Yi Tian, Jin-Wen Liu, Jian College of Electronic Information Engineering South-Center University for Nationalities Wuhan 430074 China Key Laboratory of Education Huazhong University of Science and Technology Ministry for Image Processing and Intelligent Control Wuhan Hubei 430074 China

ISBN: (纸本)0780390067

An efficient generic architecture for two-dimensional discrete wavelet transform (2-D DWT) with line-based method is proposed with using lifting scheme, in which the parallelism of four subbands transform in lifting-based 2-D DWT is exploited. The proposed architecture is designed to generate 4 subbands coefficients concurrently per clock cycle that can perform a 1-level decomposition of a N×N image in approximately N2/4 working clock cycles, which has faster throughput rate but requires less hardware cost compared to the designs reported in previous literature. © 2005 IEEE.

关键词： Parallel processing systems

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A framework for spatial-variant thresholding of digital subtraction angiography images

A framework for spatial-variant thresholding of digital subt...

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MIPPR 2005: Geospatial Information, Data Mining, and Applications

作者： Sang, Nong Peng, Weixue Li, Heng Zhang, Tianxu Institute for Pattern Recognition and Artificial Intelligence Huazhong University of Science and Technology Wuhan 430074 China Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan 430074 China

Vessel segmentation is the base of 3d reconstruction of Digital Subtraction Angiograph (DSA) images. This paper proposes a framework of adaptive local thresholding based on a verification-based approach for vessel segmentation of DSA images. The original DSA image is firstly divided into overlapping subimages according to a priori knowledge of the diameter of vessels. We implement a hypothesis test to determine whether each subimage contains vessels and then choose an optimal threshold respectively for every subimage previously determined to contain vessels, with a secondary verification process to exclude the condition that the subregion only containing the background but misclassified as one containing vessels by the hypothesis test. Finally an overall binarization of the original image is achieved by combining the thresholded subimages. Experiments demonstrate superior performance over global thresholding and some adaptive local thresholding methods.

关键词： Angiography

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Distributed Resource Allocation via Accelerated Saddle Point Dynamics

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IEEE/CAA Journal of Automatica Sinica 2021年第9期8卷 1588-1599页

作者： Wen-Ting Lin Yan-Wu Wang Chaojie Li Xinghuo Yu School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074 Key Laboratory of Image Processing and Intelligent Control Ministry of EducationHuazhong University of Science and TechnologyWuhan 430074China School of Electrical Engineering and Telecommunications University of New South WalesSydney NSW 2052Australia School of Engineering Royal Melbourne Institute of Technology(RMIT University)Melbourne VIC 3000Australia

In this paper,accelerated saddle point dynamics is proposed for distributed resource allocation over a multi-agent network,which enables a hyper-exponential convergence ***,an inertial fast-slow dynamical system with vanishing damping is introduced,based on which the distributed saddle point algorithm is *** dual variables are updated in two time scales,i.e.,the fast manifold and the slow *** the fast manifold,the consensus of the Lagrangian multipliers and the tracking of the constraints are pursued by the consensus *** the slow manifold,the updating of the Lagrangian multipliers is accelerated by inertial ***-exponential stability is defined to characterize a faster convergence of our proposed algorithm in comparison with conventional primal-dual algorithms for distributed resource *** simulation of the application in the energy dispatch problem verifies the result,which demonstrates the fast convergence of the proposed saddle point dynamics.

关键词： Accelerated saddle point dynamic distributed resource allocation hyper-exponential stability power dispatch

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