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2005 27th Annual International Conference of the engineering in Medicine and Biology Society, IEEE-EMBS 2005

作者： Mi, Yan Sun, Caixin Yao, Chenguo Xiong, Lan Wang, Shibin Li, Chengxiang Li, Jun Hu, Lina Key Lab. of High Voltage Engineering and Electrical New Technology Ministry of Education Chongqing University Chongqing 400044 China Chongqing University of Medical Sciences Chongqing 400010 China

ISBN: (纸本)0780387406

This paper discussed the lethal effects of Steep Pulsed Electric Field (SPEF) to target lymphatic capillaries in VX2 implanted breast cancer of rabbits using methylene blue dye injection staining, 5'-AMP-ALPase enzyme histochemical double staining, electron microscopy and VEGF-C observations. The result of methylene blue dye perfusion showed that the edge of tumor treated with SPEF was indistinet, no circularity or liner structure. Enzyme histochemical staining examination displayed that no positive staining was found in SPEF-target region of breast tumor, and lymphatic capillaries presented piecemeal structure in the edge of target region. Transmission electron microscopic examination showed that the continuity and integrality of lymphatic capillaries in tumor were destroyed after the treatment of SPEF. The expression of VEGF-C decreased markedly. The experiment indicated that SPEF induced the severe destruction of tumor cell and lymphatic capillaries in target region, and inhibited proliferation of lymphatic. Therefore it can decrease the possibility of lymphatic metastasis. © 2005 IEEE.

关键词： Blood vessels

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Research on platelet adsorption behavior using enzyme immunoassays and LDH testing

Research on platelet adsorption behavior using enzyme immuno...

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作者： Zhao, A.S. Yang, P. Leng, Y.X. Sun, H. Wang, J. Wan, G.J. Huang, N. Laboratory of Surface Engineering of Artificial Organs of Sichuan Key Lab. for Advanced Technologies of Materials of Ministry of Education Southwest Jiaotong University Chengdu 610031 China Institute of Surface Modification National Biomaterials Engineering Research Center China

Platelet adhesion and activation restrict the clinical applicability of blood-contacting biomaterial because platelet-biomaterial interaction can result in the formation of a haemostatic plug or thrombus. In this study we used LDH (lactate dehydrogenase) tests to evaluate the adsorption behavior of platelets on material surfaces. Enzyme immunoassay (EIA) was applied to evaluate platelet activation using a special monoclonal antibody directly binding to the P-selectin on the activated platelet membrane. The results show compared with the conventional detecting tools of platelet adhesion / activation such as optical microscopy. LDH (lactate dehydrogenase) testing and EIA (enzyme immunoassay) are surface-sensitive methods for the investigation of various aspects of platelet adsorption and activation on different biomaterials.

关键词： Platelets

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Study on the synthesis of selenium polymer by Grignard method

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Wuhan Ligong Daxue Xuebao/Journal of Wuhan University of Technology 2005年第4期27卷 19-22页

作者： Mao, Yu-Lan Huang, Ke-Zhi Gong, Yun Key Lab. for Silicate Material Science and Engineering of Ministry of Education Wuhan Univ. of Technology Wuhan 430070 China

Aliphatic and anumatic selenium polymers were synthesized with saturated dihalide and dihalogenated arene as major material by Grignard method. The effects of several factors on synthesizing reaction were studied, such as catalyst, activity of halohydrocablon, mixing order, etc. The mechanism of synthesizing reaction was researched preliminaryly.

关键词： Grignard method Selenium polymer Synthesis

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Influence of characteristics of titanium oxide film on the biological behavior of cultured human umbilical vein endothelial cells (Huvec)

Influence of characteristics of titanium oxide film on the b...

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作者： Chen, J.Y. Leng, Y.X. Ge, S.N. Yang, P. Wang, J. Sun, H. Wan, G.J. Zhao, A.S. Huang, N. Lab. of Surface Engineering of Artificial Organs of Sichuan Key Laand. for Advanced Technologies of Materials of Ministry of Education Southwest Jiaotong University Chengdu 610031 China Institute of Surface Modification National Biomaterials Engineering Research Center China

In the work reported here, titanium oxide film were synthesized using magnetron sputtering from a high-purity Ti metal target while sustaining a differential oxygen partial pressure of from 0.02 to 0.30 Pa. The biological behavior of endothelial cells grown on the film surface was studied by in vitro human umbilical vein endothelial cell (HUVEC) culture investigation. Our studies revealed that the adherence, growth, and proliferation of endothelial cells on different Ti-O film surfaces were strongly affected by the oxygen partial pressure. The biocompatibility mechanisms of ECs on Ti-O film surfaces were explored using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), α-step profilometry, scanning electron microscopy (SEM), and atomic force microscopy (AFM). We conclude that the various film characteristics such as structure, composition and surface morphology due to the different oxygen partial pressures significantly influence the biological behavior of EC.

关键词： Biomedical engineering

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The Potential Mechanisms Underlying Aspirin-induced Inhibition of Ovarian Tumor Cell Growth

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生物医学工程学杂志 2005年第S1期22卷 145-147页

作者： Yu LIU~1 Jin KE~2 Shi-Quan LIU~1 Fu-Xiang ZHOU~1 Cong-Hua XIE~1 Yun-Feng ZHOU~(1△)1(Department of Radio-Chematherapy of Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China)2(key lab. for Oral Biomedical engineering of ministry of education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China) Department of Radio- Chematherapy of Zhongnan Hospital and Cancer Research Center Wuhan University Wuhan 430071 China Key Lab. for Oral Biomedical Engineering of Ministry of Education School & Hospital of Stomatology Wuhan University Wuhan 430079 China

1 IntroductionOvarian cancer remains the most lethal disease of the gynecological cancers. Owing to the lack of an effective screening approach combined with inadequate therapeutic approach for advanced disease, fewer than 25% of ovarian cancers are identified at an early curable stage. Thus these make ovarian cancer a strong candidate for chemoprevention. In 2001, Akhmedkhanov et al. demonstrated a 2-3 folds decrease in epithelial ovarian cancer associated with Aspirin use. These epidemiological observations suggest that an improved understanding of the mechanisms by which NSAID may decrease the development of ovarian cancer could lead to improved approaches for chemoprevention of this deadly disease. In this research, we explored the potential mechanism underlying epidemiological observations that ovarian cancer occurs at a lower frequency in women exposed to Aspirin(ASP).

关键词： In Cell The Potential Mechanisms Underlying Aspirin-induced Inhibition of Ovarian Tumor Cell Growth COX

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High power high efficiency all-solid-state quasi-continuous-wave tunable Ti: Sapphire laser system

High power high efficiency all-solid-state quasi-continuous-...

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High-Power Lasers and Applications III

作者： Zou, Lei Ding, Xin Wen, Wu-Qi Zou, Yue Ma, Hong-Mei Wang, Peng Yao, Jian-Quan College of Precision Instrument and Opto-electronics Engineering Institute of Laser and Optoelectronics Tianjin University Tianjin China Key Lab. of Opto-electronics Information Science and Technology Ministry of Education Tianjin China Cooperated Institute of Nankai University Tianjin University Tianjin China

This paper reports a high output power, high conversion efficiency, all-solid-state, quasi-continuous-wave Ti: sapphire laser system pumped by frequency-doubled Nd: YAG laser with DPL and by employing it as a pump source, we got the tunable broadband wavelength varying from 750nm to 950nm. Comparing with correlative research fields, two uppermost improvements were achieved, including the topmost output power and its broadband tuning. To ensure the ideal broadband output of Ti: sapphire laser, two sets of Ti: sapphire resonator mirrors were used respectively One is from 750 to 850nm, the other is from 850 to 950nm. Because the centric wavelength of the resonator laser is nearly 795nm, the maximum of the Ti: sapphire laser output power we obtained was 6W with the first set of mirror and the transmission rate of the output mirror is 10%. The power tops this field so far and the higher conversion efficiency is 22.2%. The second set of mirror we used can generate the output power of 3W, which is high enough to achieve broadband tuning for the future. And then, by using a tunable and line width compressed implement-birefringent filter which was Brewster angle placed, we achieved continuous tuning from 750nm to 950nm with reasonably high power.

关键词： Solid state lasers

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Study on CW Nd: YAG Laser at 1319nm and 660nm

Study on CW Nd: YAG Laser at 1319nm and 660nm

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High-Power Lasers and Applications III

作者： Wang, Tao Yao, Jian-Quan Zhao, Peng Cai, Bin-Jing Wang, Peng College of Mechanism Engineering Hebei University of Technology Tianjin 300130 College of Precision Instrument and Opto-Electronics Engineering Institute of Laser and Optoelectronics Tianjin University Tianjin 300072 China Cooperated Institute Nankai University Tianjin University Tianjin 300072 China Key Lab. of Optoelectronics Information Science and Technology Ministry of Education Tianjin University Tianjin 300072 China

A dual-wave Quasi-CW Nd:YAG laser has been demonstrated at the wavelengths of 1319nm and 660nm. The radiation energy level of the 1319nm transition was analyzed. The critical technology of restraining resonance of 1064nm so as to improve that of 660nm was discussed. Sophisticated optical coating and cavity structure was studied. And a maximum CW Output power of 43W at1319nm was acquired. Based 1319nm laser, a intracavity frequency-doubling laser of 660nm was also demonstrated by using KTP crystal and a acousto-optically Q-switch. And a Quasi-CW red light output power of 2W at 660nm was acquired, accordingly, dual-wave output was realized.

关键词： Neodymium lasers

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The study of stability on a laser-diode-pumped high-power high-repetition-rate intracavity frequency doubled 532nm laser

The study of stability on a laser-diode-pumped high-power hi...

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High-Power Lasers and Applications III

作者： Zhao, Shi-Yong Yao, Jian-Quan Xu, De-Gang Zhou, Rui Zhang, Bai-Gang Zhou, Jia-Ning Wang, Peng College of Precision Instrument and Opto-Electronics Engineering Institute of Laser and Optoelectronics Tianjin University Tianjin 300072 China Cooperated Institute of Nankai University Tianjin University Tianjin 300072 China Key Lab. of Optoelectric Information Science and Technology Ministry of Education Tianjin University Tianjin 300072 China

High power laser-diode-pumped 532nm laser sources (including continuous wave and high repetition rate operation) are directly used for precise processing of metals and plastics. Furthermore, high power green laser will be used in some fields such as ocean exploration, laser probe and underwater communication. Recently, we reported a HOW diode-side-pumped Nd:YAG intracavity frequency doubled high stability 532nm laser. In the experiment, we found that the average output power of second harmonic fluctuated acutely with the variety of pumping current. Moreover, the length of arms between the mirrors were very sensitive to this cavity. We consider that one of the reason is the focus length of thermal lens of Nd:YAG rod alter with the variational pumping current, which makes the cavity be unstable. We consider the KTP crystal as a thin lens for its short length. As thermal lensing effect of the Nd:YAG rod is quite severe, so we consider it as thermal lensing medium. By ray matrix methods, we have obtained the stable regions and beam waist radii distribution in the flat-concave cavity. In our experiment, we used a pump head consisting of 80 diode bars with pentagon pump model and employed flat-concave cavity structure in order to achieve high stability output and increase output power. The total cavity length is 505mm. By using an acousto-optic Q-switching with high diffraction loss and the KTP crystal which is type n phase matching, 110 W high stability 532nm laser is achieved. The experimental result is in good agreement with the calculation.

关键词： Semiconductor lasers

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Effect of moisture and fungal exposure on the mechanical properties of hem-fir plywood

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Journal of Forestry Research 2005年第4期16卷 299-300页

作者： WANG Wei-hong Scott Kent Camille Freitag Rorbert J. leichti Jeffrey J. Morrell Key Lab. of Bio-based Material Science and Technology of Ministry of Education Northeast Forestry University Harbin 150040 P. R. China Department of Wood Science and Engineering Oregon State University USA

Hem-fir plywood were exposed to two brown rot fungi, Gloeophyllum trabeum and Postia placenta, and one white rot fungus, Trametes versicolor, to investigate the effect of fungal decay on mechanical properties of plywood. Results showed that modulus of rupture （MOR） and modulus of elasticity （MOE） of hem-fir plywood declined significantly by inoculating fungi, and weight loss of sample had a modest decrease. The fungi also made a greater effect on MOR than on MOE. Of three fungi, Postia placenta caused a most significant weight loss, and Gloeophyllum trabeum resulted in a largest flexural properties loss. Substantial declines in MOR and MOE of hem-fir plywood were also observed when the plywood samples were stored under wet conditions over 15 weeks, even in the absence of fungal attack.

关键词： Fungus decay Weight loss Flexural properties Moist condition Plywood MOR, MOE

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Correction of myocardial artifact due to limited spatial resolution in PET

Correction of myocardial artifact due to limited spatial res...

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International Conference on Machine Learning and Cybernetics (ICMLC)

作者： Hong Zhang Zhen-Xi Zhang Lin Yang Xin-Rong Ma Lab. of Biomed. Inf. Eng. of Ministry of Educ. of China Xi'an Jiaotong Univ. China The Key Laboratory of Biomedical Information Engineering of Ministry of Education of China School of Life Science and Technology Xi'an Jiaotong University China Cardiology Department The First Hospital Xi'an Jiaotong University China Department of Nuclear Medicine Xijing Hospital Xi'an China

A kind of artifact in myocardial positron emission tomography (PET) is the count loss due to the limited spatial resolution of PET, which is also named as partial volume effect (PVE). A deconvolution method was developed to correct for the artifact, in which the counts were evaluated as a parameter in the convolution. One-dimensional (1D) and two-dimensional (2D) models were used to create the convolution equation. Counts, background, myocardial wall position and thickness were parameters of the equation. The method needs non-linear fitting by an iterative process. Computer simulated myocardial images, PET image of phantoms and patient's myocardial PET images were used for evaluating the method. For simulated images, the corrected recovery coefficient is between 0.97 and 1.10 for 2D model and is near 0.9 for 1D model. 2D model can be applied for myocardium as thin as 0.25 times of the system spatial resolution while 1D model is only for 1.5 times of it. For phantom images, the recovery coefficient of 2D is near 1.0 for different thickness of the myocardium. The iterating process converges for a wide range of different size of myocardium and noise levels. 2D model allows correction for PVE exactly even for very thin myocardium.

关键词： Myocardium Spatial resolution Positron emission tomography Convolution Nonlinear equations Computational modeling Imaging phantoms Deconvolution Iterative methods Computer simulation

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