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DIA-based quantitative proteomic analysis on porcine meat quality at different chilling rates

Food Science and Human Wellness 2024年第5期13卷 2573-2583页

作者： Yuqiang Bai Tongjing Yan Fei Fang Xin Li Su Wang Juan Li Chengli Hou Dequan Zhang Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process Ministry of Agriculture and Rural AffairsInstitute of Food Science and TechnologyChinese Academy of Agricultural SciencesBeijing 100193China

The objective of this study was to evaluate the effects of chilling rate on porcine meat quality from the perspective of proteome using data independent acquisition(DIA)-based quantitative proteomic strategy. M. longissimus thoracis et lumborum(n = 9) was assigned randomly to the control group(3.72 ℃/h), very fast chilling-Ⅰ group(VFC-Ⅰ, 9.31℃/h) and VFC-Ⅱ group(14.43 ℃/h). The DIA was used to analyze the difference in proteins under different chilling rates. Results showed that tenderness was improved significantly in meat at the chilling rate of 14.43 ℃/h. Seventy-nine differential abundant proteins(fold change > 1.5, P < 0.05), including 46 up-regulated and 33 down-regulated proteins, were identified and mainly involved in carbon metabolism, pyruvate metabolism and proteasome pathways. These pathways indicated that VFC delayed cell metabolism and glycolysis by down-regulating the expression of metabolic enzymes. The tenderness was improved by up-regulating the expression of proteasome and m-calpain.

关键词： Very fast chilling Tenderness Proteomic Glycolysis Proteasome

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Comprehensive analyses of inhibition of the Chinese traditional food-packaging materials,Indocalamus latifolius leaves(Zongye)on Aspergillus flavus

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Food quality and safety 2024年第4期8卷 720-739页

作者： Wenjing Peng Xiaoxu Ren Haiting Dai Bowen Tai Bolei Yang Gang Wang Xu Li Fuguo Xing College of Food Science and Engineering Tianjin University of Science and TechnologyTianjinChina Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process Ministry of Agriculture and Rural Affairs/Institute of Food Science and TechnologyChinese Academy of Agricultural SciencesBeijingChina

Objectives:The contamination of Aspergillus flavus and aflatoxins(AFs)is one of the most serious safety problems in food and *** discovery and application of plant-sourced antifungal agents are hot topics in mycotoxin *** this study,we aim to develop efficient strategies to control *** and subsequent contamination by *** and Methods:We focused on Zongye essential oil,which was extracted from Indocalamus latifolius leaves(Zongye,the traditional food-packaging materials).Its antifungal and antitoxin activities were observed by volatile test,and the significant morphological and ultrastructural variations were observed by scanning electron microscopy(SEM)and transmission electron microscopy(TEM)*** main components of Zongye essential oil were analyzed by gas chromatography-mass spectrometry(GC-MS).RNA sequencing(RNA-Seq)and real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)analyses were used to reveal the inhibitory ***:Among them,No.2 Zongye essential oil completely inhibited the growth and toxin production of *** after 10μL *** Zongye essential oil treatment,evidently morphological and ultrastructural variations were observed,such as hyphae shrinkage,partial distortion,and a decrease in conidia *** and linalool were the major constituents of Zongye essential oil,accounting for 9.55% and 7.95%,respectively,and linalool had stronger inhibitory effects on fungal growth and mycotoxin biosynthesis than *** on the experimental results,the antifungal mechanism was proposed:down-regulations of membrane proteins(AFLA_005560,AFLA_019420,and AFLA_084310,etc.)and conidial genes(fblC,steA,and abaA,etc.)inhibited fungal development,and anti-aflatoxigenic activity might be due to significant down-regulation of AF clustergenes andglobal regulators(AtfA and AtfB).Conclusion:A novel antifungal agent,Zongye essential oil,was identified,and its inhibitory mechanis

关键词： Indocalamus latifolius Zongye essential oil Aspergillus flavus aflatoxin inhibitory mechanism

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Salicylic acid treatment inhibits cold-induced sweetening by regulating the transcription factors StAP2/ERF and StNAc2 in potato

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Food quality and safety 2024年第4期8卷 805-814页

作者： Jiadi Zhang Qiong Lin Linli Mao Ting Min Wenqiang Guan Xin Qi Hongxun Wang School of Food Science and Engineering Wuhan Polytechnic UniversityWuhanChina School of Food Science and Engineering Northwest UniversityXianChina Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process Ministry of Agriculture and Rural Affairs/Institute of Food Science and TechnologyChinese Academy of Agricultural SciencesBeijingChina Tianjin Key Laboratory of Food Biotechnology College of Biotechnology and Food ScienceTianjin University of CommerceTianjinChina Tianjin Academy of Agricultural Sciences TianjinChina

Cold-induced sweetening(CIS)results in browning and acrylamide formation in fried potato products.β-Amylase(BAM)and vacuolar invertase(INV)are crucial for starch-sugar conversion in cold-stored *** research investigates the effects of salicylic acid(SA)treatment on the CiS of potatoes and gene regulation during this *** findings revealed that the SA treatment significantly alleviated CiS by suppressing StBAM1 and St/NV1 expression in ***,the expression of two transcription factors(TFs),StAP2/ERF and StNAC2,was inhibited by SA *** overexpression of StAP2/ERF(AP2:APETALA2;ERF:ethylene responsive factor)and StNAC2 in tobacco leaves increased the expression of NtBAM1 and NtiNV,thus promoting starch-sugar ***,the β-glucuronidase(GUS)activity demonstrated that StAP2/ERF and StNAC2 increased the promoter activity of StBAM1 and *** study identifies new TFs in CIS regulation and provides new ideas for alleviating CiIS inpotato.

关键词： Potato cold-induced sweetening(CIS) salicylic acid transcription factors post-harvest storage

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Neuroprotective effects of Longxue Tongluo Capsule on ischemic stroke rats revealed by LC-MS/MS-based metabolomics approach

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Chinese Herbal Medicines 2023年第3期15卷 430-438页

作者： Jing Sun Xianyang Chen Yongru Wang Yuelin Song Bo Pan Bei Fan Fengzhong Wang Xiaonan Chen Pengfei Tu Jiarui Han Huixia Huo Jun Li Modern Research Center for Traditional Chinese Medicine School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing 100029China Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process Laboratory of Agro-products Quality Safety Risk AssessmentMinistry of AgricultureInstitute of Food Science and TechnologyChinese Academy of Agricultural SciencesBeijing 100193China Baofeng Key Laboratory of Genetics and Metabolism Zhongguancun Biological and Medical Big Data CenterBeijing 101300China

Objective:The present study aimed to evaluate the therapeutic effect and explore the underlying mechanisms of Longxue Tongluo Capsule(LTC)on ischemic stroke ***:Twenty-six rats were randomly divided into four groups,including sham group,sham+LTC group,MCAO group,and MCAO+LTC *** stroke rats were simulated by middle cerebral artery occlusion(MCAO),and LTC treatment group were orally administrated with 300 mg/kg of LTC once daily for seven consecutive *** therapy was validated in terms of neurobehavioral abnormality evaluation,cerebral infarct area,and histological *** plasma metabolome comparisons amongst different groups were conducted by UHPLC-Q Exactive MS in combination with subsequent multivariate statistical analysis,aiming to finding the molecules in respond to the surgery or LTC ***:Intragastric administration of LTC significantly decreased not only the neurobehavioral abnormality scores but also the cerebral infarct area of MCAO *** interstitial edema,atrophy,and pyknosis of glial and neuronal cells occurred in the infarcted area,core area,and marginal area of cerebral cortex were improved after LTC treatment.A total of 13 potential biomarkers were observed,and Youden index of 11 biomarkers such as LysoPC,SM,and PE were more than 0.7,which were involved in neuroprotective *** correlation and pathway analysis showed that LTC was beneficial to ischemic stroke rats via regulating glycerophospholipid and sphingolipid metabolism,together with nicotinate and nicotinamide *** and ternary analysis indicated the synergistic effect of carbohydrates and lipids may be induced by flavonoid intake from ***:The present study could provide evidence that metabolomics,as systematic approach,revealed its capacity to evaluate the holistic efficacy of TCM,and investigate the molecular mechanism underlying the clinical treatment of LTC on ischemic stroke.

关键词： ischemic stroke Longxue Tongluo Capsule metabolomics middle cerebral artery occlusion phenolic compounds UHPLC-Q Exactive MS

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Proteomic analysis of pathogen-responsive proteins from maize stem apoplast triggered by Fusarium verticillioides

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Journal of Integrative Agriculture 2022年第2期21卷 446-459页

作者： Hafiz ABDUL HASEEB ZHANG Jun GUO Yu-shuang GAO Mei-xu GUO Wei Institute of Food Science and Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural AffairsBeijing 100193P.R.China Key Laboratory of Molecular Genetics China National Tobacco CorporationGuizhou Institute of Tobacco ScienceGuiyang 550081P.R.China

During the attack of a pathogen, a variety of defense-associated proteins are released by the host plant in the apoplast to impede the perceived attack. This study utilized the mass spectrometry(LC-MS/MS) and label-free quantification method to analyze the apoplastic fluid(APF) from maize stalk and identified the proteins responsive to the Fusarium verticillioides infection. We have identified 742 proteins, and among these, 119 proteins were differentially accumulated(DAPs), i.e., 35 up-regulated, 18 down-regulated, and 66 proteins were only induced by the pathogen infection. The differentially accumulated proteins were analyzed for their Gene Ontology(GO) and Kyoto Encyclopedia of Gene and Genomes(KEGG) pathway enrichment. The highly enriched Biological process(BP) term was the L-serine biosynthesis process, whereas the most enriched Molecular Function(MF) term was the cysteine-type endopeptidase inhibitor activity. It was also found that the pathways related to the biosynthesis of amino acid, biosynthesis of secondary metabolites, protein processing in the endoplasmic reticulum, and carbohydrate metabolic pathways were significantly enriched. Moreover, 61 out of 119 differentially accumulated proteins were predicted as secretory proteins. The secretory pathways analysis showed that a greater number of proteins were secreted through the conventional secretion system compared to the unconventional secretion system. The identified secreted proteins were related to a variety of pathways in defense responses including cell redox homeostasis, cell wall modification, signal transduction, carbohydrate metabolism, binding proteins(metal ion binding, RNA binding and heme-binding), maintenance and stabilization of other proteins, indicating a complex response from the plant to the fungal infection. Our data suggested that a number of host proteins belonging to various pathways have been modulated in the apoplastic region.

关键词： apoplastic proteins maize defense response proteome Fusarium verticillioides

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Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis

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Journal of Advanced Research 2024年 72卷 85-95页

作者： Wang, Gang Wu, Wenqing Keller, Nancy P. Guo, Xu Li, Erfeng Ma, Junning Xing, Fuguo Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process Ministry of Agriculture and Rural Affairs Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing 100193 China Horticulture and Landscape College Tianjin Agricultural University Tianjin 300392 China Department of Medical Microbiology and Immunology University of Wisconsin-Madison Madison 53706 WI United States

Introduction: Ochratoxins (OTs) are worldwide regulated mycotoxins contaminating a variety of food-environment and agro-environment. Several Aspergillus and Pencillium species synthesize OTs from a six-gene biosynthetic gene cluster (BGC) to produce the highly toxic final product OTA. Although many studies on OTA-degrading enzymes were performed, high efficiency enzymes with strong stability are extremely needed, and the OTA degrading mechanism is poorly understood. Objectives: The study aimed to explore the OT-degradation enzyme and investigate its degradation mechanisms in Metarhizium, which contain an OT biosynthetic gene cluster. Methods: Phylogenomic relationship combined with RNA expression analysis were used to explore the distribution of OT BGC in fungi. Bioactivity-guided isolation and protein mass spectrometry were conducted to trace the degrading enzymes in Metarhizium spp., and the enzymes were heterologously expressed in E. coli and verified by in vitro assays. Structure prediction and point mutation were performed to reveal the catalytic mechanism of MbAmh1. Results: Beyond Aspergillus and Pencillium species, three species of the distant phylogenetic taxon Metarhizium contain an expressed OT-like BGC but lack an otaD gene. Unexpectedly, no OT BGC products were found in some Metarhizium species. Instead, Metarhizium metabolized both OTA and OTB to their non-toxic degradation products. This activity of M. brunneum was attributed to an intracellular hydrolase MbAmh1, which was tracked by bioactivity-guided proteomic analysis combined with in vitro reaction. Recombinant MbAmh1 (5 μg/mL) completely degraded 1 μg/mL OTA within 3 min, demonstrating a strong degrading ability towards OTA. Additionally, MbAmh1 showed considerable temperature adaptability ranging from 30 to 70 °C and acidic pH stability ranging from 4.0 to 7.0. Identification of active sites supported the crucial role of metal iron for this enzymatic reaction. Conclusion: These findings reveal d

关键词： Detoxification Mycotoxin Ochratoxigenic fungi OTA Secondary metabolite

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Development of an efficient CRISPR/Cas9 system in Fusarium verticillioides and its application in reducing mycotoxin contamination

Development of an efficient CRISPR/Cas9 system in Fusarium v...

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2024年亚洲植物病理学大会

作者： Tingting Tang Yi Ding Wei Guo Institute of Food Science and Technology Chinese Academy of Agricultural SciencesKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs

关键词： CRISPR Development of an efficient CRISPR/Cas9 system in Fusarium verticillioides and its application in reducing mycotoxin contamination

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Development of an Efficient CRISPR/Cas9 System in Fusarium verticillioides and Its Application in Reducing Mycotoxin Contamination

Development of an Efficient CRISPR/Cas9 System in Fusarium v...

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中国植物病理学会2024年学术年会

作者： TANG Tingting DING Yi GUO Wei Institute of Food Science and Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs

Fusarium verticillioides is a globally recognized and highly impactful fungal pathogen of maize,causing yield losses and producing harmful mycotoxins that pose a threat to human and animal ***,the genetictools available for studying this crucial fungus are currently limited in comparison with other important fungal *** address this,an efficient CRISPR/Cas9 genome editing system based on an autonomously replicating plasmid with an AMA1 sequence was established in this ***,gene disruption ofpyrG and pyrE via non-homologous end-joining（NHEJ） pathway was successfully achieved,with efficiency ranging from 66% to 100%.Secondly,precise gene deletions were achieved with remarkable efficiency using a dual sgRNA expression ***,the developed genome editing system can be applied to generate designer chromosomes in ***,as evidenced by the deletion of a crucial 38 kb fragment required for fumonisin ***,the pyrG recycling system has been established and successfully applied in ***,the developed ΔFUM1 and ΔFUM mutants can serve as biocontrol agents to reduce the Fumonisin B（FB） contamination produced by the *** together,these significant advancements in geneticmanipulation and biocontrol strategies provide valuable tools for studying and mitigating the impact of *** on maize crops.

关键词： CRISPR/Cas9 Fusarium verticillioides fumonisin uracil auxotrophic selection system atoxigenic strain

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New Strategy to Improve the quality and Microstructure of Chinese Stewed Dishes (Beef with Potato): Ultrasound-Assisted Immersion Freezing Method

SSRN

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SSRN 2025年

作者： Cui, Liye Lin, Hengxun Hu, Xiaojia Li, Xia Guan, Wenqiang Jia, Wei Yang, Yiping Chen, Yong Zhang, Chunhui Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process Ministry of Agriculture and Rural Affairs Beijing100193 China Tianjin Key Laboratory of Food Biotechnology Tianjin University of Commerce Tianjin300134 China

Prepared dishes, as a technological extension of pre-conditioned foods and representative products of the central kitchen, are becoming a major part of people’s daily diets. However, the problem of dish quality deterioration owing to freezing in the frozen supply chain has not yet been addressed, and freezing quality enhancement techniques are lacking. Herein, special equipment was used to investigate effects of ultrasound-assisted immersion freezing (UIF) on the quality of the prepared dish (braised beef with potato) and compare it with immersion freezing (IF) and conventional air freezing (AF). Results indicated that UIF effectively shortened the freezing time and improved the freezing rate of the dish. A suitable intensity of UIF treatment (150 W) improved dish quality, and high-intensity UIF treatments (300 and 450 W) caused tissue structure destruction and decreased quality. The hardness of beef and potatoes treated with UIF-150 was significantly higher than that of other treatments (P © 2025, The Authors. All rights reserved.

关键词： Beef

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Residue behaviors and dietary risk assessment of dinotefuran and its metabolites in Lycium barbarum from farm to fork

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Food Science and Human Wellness 2024年

作者： Ouli Xiao Minmin Li Jieyin Chen Ran Wang Xiaofeng Dai Zhiqiang Kong State Key Laboratory for Biology of Plant Diseases and Insect Pests， Institute of Plant Protection， Chinese Academy of Agricultural Sciences Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process， Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology， Chinese Academy of Agricultural Sciences Western Agricultural Research Center， Chinese Academy of Agricultural Sciences Institute of Plant Protection， Beijing Academy of Agriculture and Forestry Sciences

Lycium barbarum， known as wolfberry or goji berry， is consumed by humans as a medicine and a food homology product. Conventionally grown wolfberry is often treated extensively with pesticides， which could pose a hazard to humans. Here， the degradation dynamics of dinotefuran and its two metabolites (UF and DN)， during wolfberry cultivation and processing was investigated. The half-life (T1/2) of dinotefuran was 11.36 d and 9.12 d， respectively， under the recommended dosage and double the recommended dosage. During the oven and sun drying processes， processing factors (PFs) of dinotefuran were 1.07 - 1.34， implying the enrichment of pesticide residues. Decoction process made the removal rate of dinotefuran reach 87.48%， which is higher than that of the brewing process (14.7%)， while dinotefuran remained in the wine with high ethanol content in the alcohol soaking process. The hazard quotient (HQ) of dinotefuran， as determined via dietary risk assessment combined with PFs， was < 1， indicating an acceptable risk for human consumption. Bioaccessibility of dinotefuran in the three digestive stages were intestinal (18.20% - 88.08%) > gastric (5.45% - 86.72%) > oral (23.18%) via in vitro simulated digestive system. These findings provide scientific evidence for reasonable application and risk assessment of dinotefuran residues in wolfberry.

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