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Metabolome profiling of stratified seeds provides insight into the regulation of dormancy in Davidia involucrata

Plant Diversity 2022年第4期44卷 417-427页

作者： Shiming Deng Qiang Xiao Cigui Xu Jian Hong Zhijun Deng Dan Jiang Shijia Luo Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu UniversityEnshi 445000Hubei ProvinceChina Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

Dove tree(Davidia involucrata),a tertiary vestige species,is well-adapted to cool *** in *** seed lasts for an extremely long period of time,typically between 3 and 4 years,and this characteristic makes the species an excellent model for studying the mechanisms of seed *** molecular mechanisms governing germination control in *** are still *** stratification have been reported to enhance germination in recalcitrant *** performed a widely targeted metabolome profiling to identify metabolites and associated pathways in *** seeds from six different moist sand stratification durations(0-30 months) using the ultra-high-performance liquid chromatography-Q Exactive Orbitrap-Mass *** was an increasing germination rate with prolonged stratification durations(12-30 months).Furthermore,we detected 10,008 metabolites in the stratified *** also detected 48 differentially accumulated metabolites(DAMs) between all stratification periods in the seeds,with 10 highly conserved *** of the differentially accumulated metabolites between unstratified and stratified seeds were enriched in purine metabolism,pyrimidine metabolism,flavone and flavonol biosynthesis,phenylpropanoid biosynthesis,and arginine biosynthesis *** phytohormones,abscisic acid,indole-3 acetic acid,and sinapic acid were differentially accumulated in the seeds and are predicted to regulate dormancy in *** have provided extensive metabolic information useful for future works on dove tree germination study.

关键词： Dove tree Metabolome Dormancy Abscisic acid Stratification

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Litter Inputs Reshape Soil Respiration Partitioning in Subalpine Forests: Pivotal Role of Microbial Gene Abundance and Root-Microbe Interactions

SSRN

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SSRN 2025年

作者： Kuang, Yuxiao Xiong, Jia Zhang, Linhui Wang, Genxu Hu, Zhaoyong Wang, Wenzhi Sun, Juying Chang, Ruiying Sun, Xiangyang Su, Yuanyuan Lin, Shan Sun, Hailong Sun, Shouqin State Key Laboratory of Hydraulics and Mountain River Engineering College of Water Resource and Hydropower Sichuan University Chengdu610065 China Hubei Key Laboratory of Biologic Resources Protection and Utilization Hubei Minzu University Enshi445000 China Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu610213 China

Subalpine forest soils are critical yet underexplored regulators of terrestrial carbon (C) cycling, where climate-driven changes in litter input may trigger nonlinear feedbacks between soil respiration (Rs) components and microbial networks. This study presents a comprehensive three-year (2019–2021) field experiment investigating the differential impacts of litter addition and removal on Rs components and soil microbial communities in coniferous and broad-leaved subalpine forests. Litter addition enhanced Rs and ahtotrophic respiration (Ra) by 9.52% and 25.33% in the coniferous forest and by 21.37% and 57.84% in the broad-leaved forest, respectively, with minimal effects on heterotrophic respiration (Rh). Conversely, litter removal significantly reduced Rs, Ra and Rh by 22.51%, 37.32% and 15.56% in the coniferous forest, and by 25.26%, 37.30% and 19.38% in the broad-leaved forest, respectively. The effects were more pronounced in broad-leaved forest. Litter addition increased fungal diversity, whereas litter removal enhanced fungal network complexity and promoted bacterial network cooperation (e.g., reduced modularity and shortened path length), ultimately reducing the stability of fungal and bacterial networks. Root exclusion amplified fungal diversity and microbial biomass C (MBC) shifts under litter manipulation, highlighted the dependence of microbial restructuring on the presence of root-derived resources. Structural equation modeling identified microbial gene abundance as the primary regulator of Rs and Rh, surpassing the effects of community composition and diversity. These findings highlight the dual role of litter in driving microbial community restructuring and respiratory partitioning, emphasizing the importance of microbial functional traits over taxonomic diversity in predicting soil C resilience under global change. © 2025, The Authors. All rights reserved.

关键词： Fungi

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Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress

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Journal of Forestry Research 2021年第2期32卷 841-853页

作者： Peipei Jiao Zhihua Wu Xu Wang Zhenbo Jiang Yanqin Wang Hong Liu Rui Qin Zhijun Li College of Life Sciences Tarim UniversityAlar 843300Xinjiang ProvincePeople’s Republic of China Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin Xinjiang Production and Construction GroupAlar 843300Xinjiang ProvincePeople’s Republic of China Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China&Key Laboratory of State Ethnic Affairs Commission for Biological Technology College of Life SciencesSouth-Central University for NationalitiesWuhan 430074Hubei ProvincePeople’s Republic of China

Populus euphratica Oliv.,the Euphrates poplar,is the tallest tree species in the arid desert areas of Northwest *** of its drought-resistance genes is valuable to increase understanding of drought resistance ***-seq of leaves and roots under drought simulation by 25%polyethylene glycol-6000(PEG 6000)were performed at 0,4,and 12 *** and roots responded differently to drought via differentially upregulated and downregulated genes;more genes were downregulated than upregulated in both leaves and ***,these differentially expressed genes were enriched in different GO terms and KEGG *** example,GO term‘response to organic substance’and KEGG pathway‘nitrogen metabolism’were enriched for drought-stressed leaves,while GO term‘cell wall organization or biogenesis’and KEGG pathway‘zeatin biosynthesis’were enriched for drought-stressed *** enrichment of the GO term‘enzyme linked receptor protein signalling pathway’in both leaf and root drought responses suggests that these tissues may also have similar ***,roots under drought stress for four hs responded by activating programed cell *** KEGG pathway‘plant hormone signal transduction’was detected for 4-and 12-h droughtstressed leaves and 12-h drought-stressed roots,suggesting that plant hormone signal transduction plays an important role in both roots and *** enrichment of upregulated and downregulated genes for leaves and roots reflect differentially regulatory mechanisms of response to drought stress via different biological processes such as the regulation of photosynthesis and auxin signalling pathway in leaves,and the regulation of defence response and water homeostasis in *** candidate genes,including transcription factors,protein kinase,transporter,late embryogenesis abundant protein and mannitol dehydrogenase,were further selected to determine their response to drought using *** results show that the expression patte

关键词： Drought stress RNA-seq Populus euphratica qRT-PCR

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Preparation and physicochemical properties of konjac glucomannan ibuprofen ester as a polysaccharide-drug conjugate (vol 58, pg 52, 2020)

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JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY 2021年第7期58卷 520-520页

作者： Kang, Huiting Guan, Lianxiong An, Kai Tian, Dating School of Chemical and Environmental Engineering Hubei Minzu University Enshi China Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu University Enshi China

A novel drug-polysaccharide conjugate with konjac glucomannan (KGM) as a drug carrier was fabricated through the esterification of ibuprofen (IBU), an anti-inflammatory drug, with KGM. The influences of the reaction conditions, such as the amount of ibuprofen acryl chloride, reaction time, reaction temperature, and the amount of catalyst, on the degree of substitution were investigated. KGM ibuprofen ester (KGM-IBU) was characterized by Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), solid-state ¹³C NMR, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and dynamic mechanical analysis (DMA). The hydrophobic structure of IBU in KGM-IBU was proven by the fluorescence emission spectra of pyrene. In addition, by using commercially available ibuprofen sustained-release capsules (IBU-SRC) as a control, the in vitro controlled release performance of KGM-IBU was evaluated. The cumulative release of IBU-SRC within 36 h was 94%, while that of KGM-IBU within 36 h was 77%. The results showed that KGM-IBU had better sustained-release performance without a burst release effect. The obtained products could be used as a potential biocompatible sustained-release drug delivery system. © 2020 Taylor & Francis Group, LLC.

关键词： drug delivery esterification ibuprofen Konjac glucomannan polysaccharide

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Simultaneous Detection of Four Specific Dnas Fragments Based on Two-Dimensional Bimetallic Mof Nanosheets

SSRN

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SSRN 2022年

作者： Wang, Peng Wang, Xiaohong Li, Wenheng Leng, Zhongming Lu, ZiJing Zhai, Kun Xiang, Dongshan Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu University Enshi445000 China School of Chemical and Environmental Engineering Hubei Minzu University Enshi445000 China School of Resources and Environmental Engineering Wuhan University of Technology Wuhan430072 China

We synthesized a bimetallic organic framework (Co/Fe(1:1) MOF) nanosheet with good fluorescence quenching effect using two metal ions Co2+, Fe3+ and terephthalic acid, a new method of one-step simultaneous detection of four specific DNA fragments based on bimetallic organic framework nanosheets were developed. It was demonstrated by an oligonucleotide sequence of wild-type HBV and three reverse transcription oligonucleotide sequences of RNA fragment of Hepatitis A virus (HAV), Hepatitis C virus (HCV) and Human immunodeficiency virus (HIV) as a model system. In this analytical method, four different dyes (FAM, TAMRA, Cy-5, and Cy-7) were labeled on the complementary sequences of four different target DNAs as fluorescent probes. In the absence of target DNAs(T-DNAs), four fluorescent probes were adsorbed on the Co/Fe (1:1) MOF by π-π stacking, hydrogen bonding, van der Waals forces and the dyes were close to Co/Fe (1:1) MOF, their fluorescence were quenched by MOF nanosheets. In the presence of T-DNAs, four fluorescent probes hybridize with the corresponding T-DNAs to form double-stranded DNAs, causing the fluorescent probes were moved away from the surface of the Co/Fe (1:1) MOF and the fluorescence signals were recovered. Under the optimal conditions, the fluorescence intensities of four dyes all exhibited good linear dependence on corresponding concentration of T-DNAs. The detection limits targets of HAV, HBV, HCV and HIV were 0.032 nmol/L, 0.037nmol/L, 0.53 nmol/L and 0.18 nmol/L (3σ, n=9), respectively. The analytical method has low detection limit, good selectivity and high sensitivity, and can be used to detect T-DNAs in complex matrices. © 2022, The Authors. All rights reserved.

关键词： Fluorescence spectroscopy

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Effect of High Alumina-Based Solid Waste on Efflorescence Behavior of Alkali-Activated Steel Slag

SSRN

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SSRN 2022年

作者： Zhang, Ruobing Zhang, Yimin Liu, Tao Wan, Qian Zheng, Dalong School of Resource and Environmental Engineering Wuhan University of Science and Technology Hubei Province Wuhan430081 China State Environmental Protection Key Laboratory of Mineral Metallurgical Resources Utilization and Pollution Control Wuhan University of Science and Technology Hubei Province Wuhan430081 China Collaborative Innovation Center of Strategic Vanadium Resources Utilization Wuhan University of Science and Technology Hubei Province Wuhan430081 China Hubei Provincial Engineering Technology Research Center of High efficient Cleaning Utilization for Shale Vanadium Resource Wuhan University of Science and Technology Hubei Province Wuhan430081 China

In this paper, the influences of efflorescence on the property of alkali-activated steel slag materials were investigated, and the (Spent Fluid Cracking Catalytic) SFCC catalyst was used as an additive to control efflorescence. The property and microstructure of alkali activated cementitious materials synthesized with different raw materials composition was characterized by XRD, FTIR, TG and SEM-EDS. The efflorescence degree of different products was also evaluated. The C-S-H was mainly formed during the alkali-activated steel slag, which is difficult to adsorb the alkali metal ions, resulting in the serious efflorescence and a reduction of compressive strength. As the addition of SFCC catalyst increased, more N-A-S-H gels formed and adsorbed alkali metal ions, while inhibiting the efflorescence and its effect on mechanical property. In addition, the [AlO 4 ] - contained in the N(C)-A-S-H exhibit stronger adsorption properties for calcium ions than for sodium ions. This study not only proposed a new method of synergistic utilization of steel slag and waste SFCC catalyst, but also provided a further understanding of efflorescence. © 2022, The Authors. All rights reserved.

关键词： Catalysts

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Syntheses, Crystal Structures and Hydrogen-bonding Motif of Two Glycoluril Derivatives

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Chinese Journal of Structural Chemistry 2019年第5期38卷 754-760,669页

作者： LIU Xue-Mei WANG Dong-Hua SONG Xin-Jian WANG Jun-Gang Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei University for Nationalities Enshi 445000 China School of Chemical Engineering Guizhou Minzu University Guiyang 550025 China

The synthesis and aggregation behavior of two glycoluril derivatives were *** crystal of compound 1 belongs to the triclinic system,space group ■,with a=8.946（1）,b=13.445（2）,c=20.361（3）?,α=89.517（3）,β=84.405（3）,γ=86.325（3）°,Z=2,V=2432.1（6）?3,Dc=1.293 g·cm-3,C48H34N6O6·2DMSO,Mr=947.07,F（000）=992,μ=0.170 mm-1,the final R=0.1606 and wR=0.2410 for 4382 observed reflections（Ⅰ>2σ（Ⅰ））.The crystal of compound 2belongs to the monoclinic system,space group P21/c,with a=9.500（3）,b=24.522（8）,c=17.997（6）?,Z=2,V=4054（2）?3,Dc=1.296 g·cm-3,C48H34N6O6,Mr=790.81,F（000）=1648,μ=0.087mm-1,the final R=0.2146 and wR=0.2535 for 2878 observed reflections（Ⅰ>2σ（Ⅰ））.Unlike the highly conserved R22（8）hydrogen-bonding amide···amide homosynthon,the structural analysis indicated that compound 1 selected the C（20）hydrogen-bonding motif and 2 selected the R22（34）hydrogen-bonding motif,which revealed the competition from remote solvent molecule and the interference of the molecular hydrocarbon core in crystal *** supramolecular structure revealed molecule 1 is further linked into a one-dimensional chain and 2 into a two-dimensional interwoven plane by N–H···N hydrogen bonds.

关键词： hydrogen bond glycoluril supramolecuclar synthons crystal engineering

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Preparation and physicochemical properties of konjac glucomannan ibuprofen ester as a polysaccharide-drug conjugate

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Journal of Macromolecular Science, Part A 2021年第1期58卷 32-43页

作者： Huiting Kang Lianxiong Guan Kai An Dating Tian a School of Chemical and Environmental Engineering Hubei Minzu University Enshi People’s Republic of China a School of Chemical and Environmental Engineering Hubei Minzu University Enshi People’s Republic of Chinab Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu University Enshi People’s Republic of China

A novel drug-polysaccharide conjugate with konjac glucomannan (KGM) as a drug carrier was fabricated through the esterification of ibuprofen (IBU), an anti-inflammatory drug, with KGM. The influences of the reaction conditions, such as the amount of ibuprofen acryl chloride, reaction time, reaction temperature, and the amount of catalyst, on the degree of substitution were investigated. KGM ibuprofen ester (KGM-IBU) was characterized by Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), solid-state13C NMR, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and dynamic mechanical analysis (DMA). The hydrophobic structure of IBU in KGM-IBU was proven by the fluorescence emission spectra of pyrene. In addition, by using commercially available ibuprofen sustained-release capsules (IBU-SRC) as a control, thein vitrocontrolled release performance of KGM-IBU was evaluated. The cumulative release of IBU-SRC within 36 h was 94%, while that of KGM-IBU within 36 h was 77%. The results showed that KGM-IBU had better sustained-release performance without a burst release effect. The obtained products could be used as a potential biocompatible sustained-release drug delivery *** Abstract

关键词： Konjac glucomannan ibuprofen esterification drug delivery polysaccharide

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Intermolecular Hydrogen Bond Modulating the Selective Coupling of Protons and Co2 to Ch4 Over Nitrogen-Doped Carbon Layers Modified Cobalt

SSRN

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SSRN 2022年

作者： Ma, Minzhi Chen, Jiahao Huang, Zeai Fa, Wenjun Wang, Fang Rao, Zhiqiang Wang, Rui Zhang, Ruiyang Cao, Yuehan Zou, Yanzhao Zhou, Ying State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum University Chengdu610500 China Institute of Carbon Neutrality School of New Energy and Materials Southwest Petroleum University Chengdu610500 China Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province College of Advanced Materials and Energy Xuchang University Henan 461000 China Department of Petroleum Engineering Kazan Federal University Kazan420008 Russia Hubei Key Laboratory of Biologic Resources Protection and Utilization China

Photocatalytic reduction of CO 2 with H 2 O to CH 4 is a promising route to complete the carbon neutrality goal. Nevertheless, one of the biggest challenges for this elegant strategy is that the coupling of the protons and CO 2 to form CH 4 is fiercely competed with proton-proton couplings to form H 2 or CO generation, leading to extremely low CH 4 selectivity. Herein, we designed and fabricated the nitrogen-doped carbon layers modified cobalt (N-C@Co) photocatalyst for modulating the selective coupling of protons and CO 2 to CH 4 during photocatalytic reduction of CO 2 . In situ DRIFTS and DFT calculations revealed that the formation of intermolecular hydrogen bond between the as-prepared N-C@Co and H 2 O molecule was found to suppress the mass transfer of the generated protons and promote the adsorption of CO 2 molecule. More crucially, it was conducive to suppress the desorption of the CO intermediate product that was typically recognized as the decisive specie for CH 4 generation. As a result, the H 2 selectivity (9.1 %) and activity (17.3 µmol g -1 ) of the as-prepared N-C@Co were reduced by a factor of 9.2 and 17.6, respectively, as compared to that of bulk Co. The CH 4 selectivity of N-C@Co was boosted 6.2 times from13.3% of bulk Co to 82.0 % of N-C@Co with the generation rate of 155.7 μmol g −1 . This work provides a fresh insight into the photocatalyst design for improving CH 4 selectivity and suppressing the competing H 2 and CO generation. © 2022, The Authors. All rights reserved.

关键词： Molecules

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Characteristics of in situ stress field in the Huainan mining area, China and its control factors

Research Square

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Research Square 2021年

作者： Shi, Xiuchang Zhang, Jixing Li, Guoqing BIM Technology and Intelligence Construction in Henan Engineering Laboratory Henan University of Economics and Law Zhengzhou450046 China Geological Survey of Jiangsu Province Nanjing210018 China School of Earth Resources China University of Geosciences Hubei Wuhan430074 China State Key Laboratory of Water Resource Protection and Utilization in Coal Mining China Energy Investment Corporation Limited Beijing100011 China Beijing100083 China

Due to the high in situ stresses, dynamic disasters occurred frequently in the Huainan mining area, China. While our understanding of the in situ stresses in this area is still insufficient. In this study, the in situ stresses of 18 sections in two boreholes in the Xinji No. 1 coalfield were measured by using hydraulic fracturing method, and the distribution of in situ stresses in the Huainan mining area were investigated. The relationship between in situ stress and geological structure in the Huainan mining area were summarized and the limitation of fault friction strength on in situ stresses were discussed. The result showed that the maximum horizontal principal stress (σH) at Xinji No. 1 mine was 13.95 - 25.23 MPa, the minimum horizontal principal stress (σh) was 12.16 - 21.17 MPa. The average azimuth of the maximum horizontal principal stress was N83.61 °E. The statistical results showed that the in situ stresses in Huainan mining area were characterized by a strike-slip faulting regime. Both the horizontal and vertical principal stresses increased approximately linearly with the increase of burial depth. The direction of the maximum principal stress in the study area is closely related to the tectonic movement and the ratio of maximum principal stress to minimum principal stress was primarily limited by the friction strength of fault. The outcomes of this research can provide some reliable engineering parameters and benefit the roadway layout and support design in the Huainan mining area. © 2021, CC BY.

关键词： Friction

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