The rapid advancements in artificial intelligence (AI) are catalyzing transformative changes in atomic modeling, simulation, and design. AI-driven potential energy models have demonstrated the capability to conduct la...
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DeePMD-kit is a powerful open-source software package that facilitates molecular dynamics simulations using machine learning potentials (MLP) known as Deep Potential (DP) models. This package, which was released in 20...
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Orthotopic liver transplantation(OLT)is the only proven effective treatment for both end-stage and metabolic liver *** transplantation is a promising alternative for OLT,but the lack of available donor livers has hamp...
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Orthotopic liver transplantation(OLT)is the only proven effective treatment for both end-stage and metabolic liver *** transplantation is a promising alternative for OLT,but the lack of available donor livers has hampered its clinical ***-like cells(HLCs)differentiated from many multi-potential stem cells can help repair damaged liver *** almost suitable cells currently identified for human use are difficult to harvest and involve invasive ***,a novel mesenchymal stem cell derived from human menstrual blood(MenSC)has been discovered and obtained easily and *** this study,we examined whether the MenSCs are able to differentiate into functional HLCs in *** three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor(HGF),fibroblast growth factor-4(FGF-4),and oncostain M(OSM),cuboidal HLCs were observed,and cells also expressed hepatocyte-specific marker genes including albumin(ALB),α-fetoprotein(AFP),cytokeratin 18/19(CK18/19),and cytochrome P450 1A1/3A4(CYP1A1/3A4).Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis,glycogen storage,and indocyanine green(ICG)*** intrasplenic transplantation into mice with 2/3 partial hepatectomy,the MenSC-derived HLCs were detected in recipient livers and expressed human ALB *** also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury *** conclusion,MenSCs may serve as an ideal,easily accessible source of material for tissue engineering and cell therapy of liver tissues.
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