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Development of a Universal RNA Dual-Terminal Labeling Method for Sensing RNA-Ligand Interactions

CCS Chemistry 2023年第1期5卷 221-233页

作者： Longhuai Cheng Dejun Ma Jie Zhang Xueying Kang Yang Wu Yi Zhao Long Yi Zhen Xi State Key Laboratory of Elemento-Organic Chemistry Department of Chemical BiologyCollege of ChemistryNational Pesticide Engineering Research CenterCollaborative Innovation Center of Chemical Science and EngineeringNankai UniversityTianjin 300071 State Key Laboratory of Organic-Inorganic Composites and Beijing Key Laboratory of Bioprocess Beijing University of Chemical Technology(BUCT)Beijing 100029 Key Laboratory of Intelligent Information Processing Research Center for Ubiquitous Computing SystemsInstitute of Computing TechnologyChinese Academy of SciencesBeijing 100190

Dual labeling of an RNA can provide Förster resonance energy transfer(FRET)sensors for studying RNA folding,miRNA maturation,and RNA-protein ***,we report the development of a highly efficient strategy for direct dual-terminal labeling of any RNA of *** explored new Michael cycloaddition for facile labeling of 5′-terminal RNA with improved *** chemical tetrazinylation of RNA at the 3′-terminus was achieved with the highly efficient and catalysis-free tetrazine-cycloalkyne *** single-terminal labeling methods were combined for dual-terminal labeling of an RNA including short hairpin RNA,pre-miRNA,riboswitch,and noncoding ***,these dual-labeled RNA-based FRET sensors were used to monitor RNA-ligand interactions in vitro and in live *** is anticipated that these universal RNA labeling strategies will be useful to study RNA structures and functions.

关键词： dual labeling Förster resonance energy transfer sensor Michael cycloaddition tetrazinecycloalkyne ligation RNA-ligand interaction

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Context-Driven Index Trimming: A data Quality Perspective to Enhancing Precision of RALMs

Context-Driven Index Trimming: A Data Quality Perspective to...

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2024 Conference on Empirical Methods in Natural Language Processing, EMNLP 2024

作者： Ma, Kexin Jin, Ruochun Wang, Haotian Wang, Xi Chen, Huan Tang, Yuhua Wang, Qian Institute for Quantum Information State Key Laboratory of High Performance Computing China College of Computer Science and Technology National University of Defense Technology Changsha China Intelligent Game and Decision Lab Academy of Military Science Beijing China

ISBN: (纸本)9798891761681

Retrieval-Augmented Large Language Models (RALMs) have made significant strides in enhancing the accuracy of generated responses. However, existing research often overlooks the data quality issues within retrieval results, often caused by inaccurate existing vector-distance-based retrieval methods. We propose to boost the precision of RALMs' answers from a data quality perspective through the Context-Driven Index Trimming (CDIT) framework, where Context Matching Dependencies (CMDs) are employed as logical data quality rules to capture and regulate the consistency between retrieved contexts. Based on the semantic comprehension capabilities of Large Language Models (LLMs), CDIT can effectively identify and discard retrieval results that are inconsistent with the query context and further modify indexes in the database, thereby improving answer quality. Experiments demonstrate average improvement of 3.75% in accuracy on challenging question-answering tasks. Also, the flexibility of CDIT is verified through its compatibility with various language models and indexing methods, which offers a promising approach to bolster RALMs' data quality and retrieval precision jointly. © 2024 Association for Computational Linguistics.

关键词： data accuracy

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Real-time Student Classroom Feedback with the RFMNet Framework in a Smart Classroom 13

Real-time Student Classroom Feedback with the RFMNet Framewo...

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13th International Conference on Information Technology in Medicine and Education, ITME 2023

作者： Tang, Xianpiao Hu, Pengyun Yu, Dewei Xia, Daoxun School of Big Data and Computer Science Guizhou Normal University Guiyang China Guizhou Normal University Guizhou Key Laboratory of Information and Computing Science Guiyang China Guizhou Normal University Engineering Laboratory for Applied Technology of Big Data in Education Guiyang China

ISBN: (纸本)9798350319156

Facial expression recognition is the ability to interpret human feelings from facial features. Teachers can use it to visualize the state of emotion and estimate students' learning effects to take corresponding intervention strategies to improve teaching. The current technology has shortcomings such as network performance degradation and feature information loss, which cannot meet the needs of sentiment analysis of student feedback. Therefore, RFMNet, a novel network model based on deep learning theory, is proposed in this paper. Featuring higher extraction ability, it is conducive to accurately analysing students' expressions. First of all, this model introduces a relation-aware global attention (RGA) module to better integrate the more discriminative expression features in the image and achieve more refined sentiment analysis. Second, the Mish activation function and the focal loss function are employed. The Mish activation function is introduced to avoid disappearing feature information due to neuron deactivation when the ReLU gradient is 0. The model achieves an average recognition accuracy of 89.62%, as confirmed through a sequence of trials conducted on the public dataset FERPlus. The model helps achieve intelligent processing of teaching information and obtaining real-time feedback by detecting, extracting, and analysing the students' facial expressions. © 2023 IEEE.

关键词： Students

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Customized scheduling for shared bus with deadlines

Customized scheduling for shared bus with deadlines

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作者： Jin, Yong Xu, Jia Xu, Lijie Liu, Linfeng Xiao, Fu Jiangsu Key Laboratory of Big Data Security and Intelligent Processing Nanjing University of Posts and Telecommunications Jiangsu Nanjing China School of Computer Science & Engineering Changshu Institute of Technology Jiangsu Changshu China

Public transportation system is one of the most effective ways to conserve energy and reduce carbon emissions. However, the traditional public transportation system does not provide customized service and cannot guarantee the arrival time to destination. To address these issues, we formulate the minimum shared bus scheduling problem to minimize the number of shared buses such that all orders can be completed under constraints of deadlines and capacity of shared bus. We propose the approximation algorithms, S-MBSA for the shared bus with strong endurance and E-MBSA for the large-scale order scenario, to solve the minimum shared bus scheduling problem. We further formulate the constrained maximum revenue shared bus scheduling problem to maximize the revenue under the limited number of shared buses, and propose an approximation algorithm, CMRBSA, to find the shared bus route schedules. Through the extensive simulations, we demonstrate the significant superiority of S-MBSA and E-MBSA in terms of number of shared buses. Furthermore, CMRBSA outperforms the benchmark algorithms significantly in terms of revenue. © 2022 John Wiley & Sons Ltd.

关键词： Scheduling

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REQUIRED NUMBER OF ITERATIONS FOR SPARSE SIGNAL RECOVERY VIA ORTHOGONAL LEAST SQUARES

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Journal of Computational Mathematics 2023年第1期41卷 1-17页

作者： Haifeng Li Jing Zhang Jinming Wen Dongfang Li Henan Engineering Laboratory for Big Data Statistical Analysis and Optimal Control College of Mathematics and Information ScienceHenan Normal UniversityXinxiang 453007China College of Information Science and Technology Jinan UniversityGuangzhou 510632China School of Mathematics and Statistics Huazhong University of Science and TechnologyWuhan 430074China Hubei Key Laboratory of Engineering Modeling and Scientific Computing Huazhong University of Science and TechnologyWuhan 430074China

In countless applications,we need to reconstruct a K-sparse signal x∈R n from noisy measurements y=Φx+v,whereΦ∈R^(m×n)is a sensing matrix and v∈R m is a noise *** least squares(OLS),which selects at each step the column that results in the most significant decrease in the residual power,is one of the most popular sparse recovery *** this paper,we investigate the number of iterations required for recovering x with the OLS *** show that OLS provides a stable reconstruction of all K-sparse signals x in[2.8K]iterations provided thatΦsatisfies the restricted isometry property(RIP).Our result provides a better recovery bound and fewer number of required iterations than those proposed by Foucart in 2013.

关键词： Sparse signal recovery Orthogonal least squares(OLS) Restricted isometry property(RIP)

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Machine Learning Based FDTD Method for 3D Electromagnetic Simulation

Machine Learning Based FDTD Method for 3D Electromagnetic Si...

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2024 IEEE International Conference on Computational Electromagnetics, ICCEM 2024

作者： Dai, Jinpeng Xie, Guoda Hou, Guilin Zhang, Liyuan Huang, Zhixiang Anhui University The Key Laboratory of Intelligent Computing and Signal Processing Hefei China Education of Anhui Province Anhui Province Key Laboratory of Target Recognition and Feature Extraction Anhui Luan237000 China Ministry of Education The Key Laboratory of Intelligent Computing and Signal Processing Hefei China Institute of Energy Hefei Comprehensive National Science Center Hefei230031 China Institute of Plasma Physics Hefei Institutes of Physical Science Chinese Academy of Sciences Hefei230031 China Anhui Province Anhui University Key Laboratory of Electromagnetic Environmental Sensing Hefei230601 China Anhui University Hefei China

ISBN: (纸本)9798350383317

This article introduces an innovative and efficient deep learning-assisted Finite-Difference Time-Domain (DL-FDTD) method in the field of computational electromagnetics. This method ingeniously integrates the Gated Recurrent Unit (GRU) network and Particle Swarm Optimization (PSO) algorithm into the traditional FDTD framework, termed as the PSO-GRU-FDTD model. A key innovation of this model is the application of the particle swarm algorithm, which significantly simplifies the parameter tuning process, thereby accelerating model development. This advancement represents a notable breakthrough from the complex parameter adjustment process typical in traditional neural network models. Moreover, compared to the traditional Long Short-Term Memory (LSTM) networks, the GRU network excels in simplicity, convenience, and efficiency, while also ensuring accuracy. Ultimately, this method is applied to three-dimensional electromagnetic simulation and emulation. Numerical results demonstrate that this approach exhibits outstanding performance in both simulation accuracy and efficiency. © 2024 IEEE.

关键词： Electromagnetic simulation

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On the parameterized complexity of minimum/maximum degree vertex deletion on several special graphs

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Frontiers of Computer science 2023年第4期17卷 97-107页

作者： Jia LI Wenjun LI Yongjie YANG Xueying YANG School of Information Engineering Hunan Industry PolytechnicChangsha 410036China Hunan Provincial Key Laboratory of Intelligent Processing of Big Data on Transportation School of Computer and Communication EngineeringChangsha University of Science and TechnologyChangsha 410015China Chair of Economic Theory Saarland UniversitySaarbrücken 66123Germany

In the minimum degree vertex deletion problem,we are given a graph,a distinguished vertex in the graph,and an integer κ,and the question is whether we can delete at most κ vertices from the graph so that the distinguished vertex has the unique minimum *** maximum degree vertex deletion problem is defined analogously but here we want the distinguished vertex to have the unique maximum *** is known that both problems areΨ-hard and fixed-parameter intractable with respect to some natural *** this paper,we study the(parameterized)complexity of these two problems restricted to split graphs,p-degenerate graphs,and planar *** study provides a comprehensive complexity landscape of the two problems restricted to these special graphs.

关键词： minimum degree maximum degree vertex deletion split graphs planar graphs parameterized complexity

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Generalized-Extended-State-Observer and Equivalent-Input-Disturbance Methods for Active Disturbance Rejection: Deep Observation and Comparison

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IEEE/CAA Journal of Automatica Sinica 2023年第4期10卷 957-968页

作者： Jinhua She Kou Miyamoto Qing-Long Han Min Wu Hiroshi Hashimoto Qing-Guo Wang School of Engineering Tokyo University of TechnologyHachiojiTokyo 192-0982Japan K.Miyamoto is with the Institute of Technology Shimizu CorporationKotoTokyo 135-0044Japan School of Science Computing and Engineering TechnologiesSwinburne University of TechnologyMelbourneVIC 3122Australia School of Automation China University of GeosciencesWuhan 430074 Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems Engineering Research Center of Intelligent Technology for Geo-Exploration Ministry of EducationWuhan 430074China School of Industrial Technology Advanced Institute of Industrial TechnologyTokyo 140-0011Japan Institute of Artificial Intelligence and Future Networks Beijing Normal UniversityZhuhai 519087 Guangdong Key Lab of AI and Multi-Modal Data Processing Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science BNUHKBU United International College Zhuhai 519087China

Active disturbance-rejection methods are effective in estimating and rejecting disturbances in both transient and steady-state *** paper presents a deep observation on and a comparison between two of those methods:the generalized extended-state observer(GESO)and the equivalent input disturbance(EID)from assumptions,system configurations,stability conditions,system design,disturbance-rejection performance,and extensibility.A time-domain index is introduced to assess the disturbance-rejection performance.A detailed observation of disturbance-suppression mechanisms reveals the superiority of the EID approach over the GESO method.A comparison between these two methods shows that assumptions on disturbances are more practical and the adjustment of disturbance-rejection performance is easier for the EID approach than for the GESO method.

关键词： Active disturbance-rejection control(ADRC) disturbance observer(DOB) equivalent input disturbance(EID) extendedstate observer(ESO) generalized extended-state observer(GESO)

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Residential Energy Consumption Forecasting Based on Federated Reinforcement Learning with data Privacy Protection

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Computer Modeling in Engineering & sciences 2023年第10期137卷 717-732页

作者： You Lu Linqian Cui YunzheWang Jiacheng Sun Lanhui Liu School of Electronic and Information Engineering Suzhou University of Science and TechnologySuzhou215009China Jiangsu Province Key Laboratory of Intelligent Building Energy Efficiency Suzhou University of Science and TechnologySuzhou215009China Chongqing Industrial Big Data Innovation Center Co. Ltd.Chongqing400707China

Most studies have conducted experiments on predicting energy consumption by integrating data formodel ***, the process of centralizing data can cause problems of data ***,many laws and regulationson data security and privacy have been enacted, making it difficult to centralize data, which can lead to a datasilo problem. Thus, to train the model while maintaining user privacy, we adopt a federated learning ***, in all classical federated learning frameworks secure aggregation, the Federated Averaging (FedAvg)method is used to directly weight the model parameters on average, which may have an adverse effect on te ***, we propose the Federated Reinforcement Learning (FedRL) model, which consists of multiple userscollaboratively training the model. Each household trains a local model on local data. These local data neverleave the local area, and only the encrypted parameters are uploaded to the central server to participate in thesecure aggregation of the global model. We improve FedAvg by incorporating a Q-learning algorithm to assignweights to each locally uploaded local model. And the model has improved predictive performance. We validatethe performance of the FedRL model by testing it on a real-world dataset and compare the experimental results withother models. The performance of our proposed method in most of the evaluation metrics is improved comparedto both the centralized and distributed models.

关键词： Energy consumption forecasting federated learning data privacy protection Q-learning

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Time Series Fusion Model for Predicting Electric power tools and equipment 6

Time Series Fusion Model for Predicting Electric power tools...

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6th International Conference on Robotics, intelligent Control and Artificial Intelligence, RICAI 2024

作者： Xue, Liang Ling, Xinghong Yang, Lei Yang, Yang Computing Science and Artificial Intelligence College Suzhou City University Suzhou China College of Computer Science and Software Engineering Hohai University Nanjing China Suzhou Key Lab of Multi-modal Data Fusion and Intelligent Healthcare Suzhou City University Suzhou China

ISBN: (纸本)9798331541699

To effectively address the issue of inadequate accuracy and timeliness in industrial data classification, this paper proposes a fusion classification model based on time series (DT-RF-MLP) for loss prediction of electrical work equipment. Firstly, data information is extracted from the dataset and features are constructed based on the data information to enhance the diversity of data features. Secondly, redundant and highly correlated features are removed by quantifying the correlation degree between the features to simplify the model inputs. On this basis, a fusion classification model is constructed and comparative experiments are conducted with a single model to validate the advantages of the fusion model in terms of prediction performance. The experimental results demonstrate that the model markedly enhances prediction accuracy while concurrently reducing the duration of the model run. © 2024 IEEE.

关键词： Prediction models

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