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A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling

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Chinese Chemical Letters 2022年第6期33卷 3091-3096页

作者： Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China The Key Laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China

The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular *** reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical *** this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination *** on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable ***,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation *** maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple *** show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La ***,statistical analysis was performed for the mean peak height and mean peak area of the cellular *** believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications.

关键词： Microfluidics Dynamic stimulation Time code High-throughput Single-cell array

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Exponential stability of impulsive positive systems with mixed time-varying delays

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IET control Theory and Applications 2014年第15期8卷 1537-1542页

作者： Wang, Yan-Wu Zhang, Ji-Shi Liu, Meng School of Automation Huazhong University of Science and Technology Wuhan430074 China School of Software Henan University Kaifeng475004 China Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China China

This study addresses the problem of exponential stability for a class of impulsive positive systems with mixed time-varying delays. A delayed impulsive positive system model is introduced for the first time and a necessary and sufficient condition guaranteeing the positivity of this kind of system is proposed. By using a copositive Lyapunov-Krasovskii functional and the average impulsive interval method, a sufficient criterion of global exponential stability for delayed impulsive positive systems is established in terms of linear programming problems. A numerical example is given to show the effectiveness of the proposed method. © 2014 The Institution of Engineering and Technology.

关键词： Lyapunov functions

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Observer-based adaptive tracking control of rigid-link flexible-joint robot manipulators

Observer-based adaptive tracking control of rigid-link flexi...

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作者： Liu, Chao Xiang, Xianbo Poignet, Philippe France Key Laboratory of Education Ministry for Image Processing and Intelligent Control Huazhong University of Science and Technology China

ISBN: (纸本)9787894631046

Joint flexibility is an important factor to consider in the robot control design if high performance is expected for the robot manipulators. Research works on control of rigid-link flexible-joint (RLFJ) robot in literature have assumed that the kinematics of the robot is known exactly. There have been few results that can deal with the kinematics uncertainty in RLFJ robot. In this paper, we propose an adaptive tracking control method which can deal with the kinematics uncertainty and uncertainties in both link and actuator dynamics of the RLFJ robot system. Nonlinear observers are designed to avoid accelerations measurement due to the fourth-order overall system dynamics. Asymptotic stability of the closed-loop system is shown and sufficient conditions are presented to guarantee the stability.

关键词： Asymptotic stability

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Optimal control of switched linear systems based on migrant particle swarm optimization algorithm

Optimal control of switched linear systems based on migrant ...

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MIPPR 2009 - Medical Imaging, Parallel processing of images, and Optimization Techniques: 6th International Symposium on Multispectral image processing and Pattern Recognition

作者： Xie, Fuqiang Wang, Yongji Zheng, Zongzhun Li, Chuanfeng Department of Control Science and Engineering Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan 430074 China

ISBN: (纸本)9780819478085

The optimal control problem for switched linear systems with internally forced switching has more constraints than with externally forced switching. Heavy computations and slow convergence in solving this problem is a major obstacle. In this paper we describe a new approach for solving this problem, which is called Migrant Particle Swarm Optimization (Migrant PSO). Imitating the behavior of a flock of migrant birds, the Migrant PSO applies naturally to both continuous and discrete spaces, in which definitive optimization algorithm and stochastic search method are combined. The efficacy of the proposed algorithm is illustrated via a numerical example. © 2009 Copyright SPIE - The International Society for Optical Engineering.

关键词： Hybrid systems

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Reaching Non-Negative Edge Consensus of Networked Dynamical Systems

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IEEE TRANSACTIONS ON CYBERNETICS 2018年第9期48卷 2712-2722页

作者： Wang, Xiao Ling Su, Housheng Chen, Michael Z. Q. Wang, Xiao Fan Chen, Guanrong College of Automation Nanjing University of Posts and Telecommunications Nanjing China School of Automation Image Processing and Intelligent Control Key Laboratory of Education Ministry of China Huazhong University of Science and Technology Wuhan China School of Automation Nanjing University of Science and Technology Nanjing China Department of Automation Shanghai Jiao Tong University and Key Laboratory of System Control and Information Processing Ministry of Education of China Shanghai China Department of Electronic Engineering City University of Hong Kong Hong Kong

In this paper, the problem of non-negative edge consensus of undirected networked linear time-invariant systems is addressed by associating each edge of the network with a state variable, for which a distributed algorithm is constructed. Sufficient conditions referring only to the number of edges are derived for non-negative edge consensus of the networked systems. Subsequently, the linear programming method and a low-gain feedback technique are introduced to simplify the design of the feedback gain matrix for achieving the non-negative edge consensus. It is found that the low-gain feedback technique has a good effect on the non-negative edge consensus of the networked systems subject to input saturation. Numerical simulations are presented to verify the effectiveness of the theoretical results.

关键词： Distributed algorithm line graph low-gain feedback networked system non-negative edge consensus

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Replaying Styles for Continual Semantic Segmentation Across Domains 7th

Replaying Styles for Continual Semantic Segmentation Across...

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7th Asian Conference on Pattern Recognition, ACPR 2023

作者： Deng, Yao Xiang, Xiang Key Lab of Image Processing and Intelligent Control Ministry of Education School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan430074 China

ISBN: (纸本)9783031476365

In the context of Domain Incremental Learning for Semantic Segmentation, catastrophic forgetting is a significant issue when a model learns new geographical domains. While replay-based approaches have been commonly used to mitigate this problem by allowing the model to review past knowledge, they require additional storage space for old data, which may not be feasible in real-world applications. To address this limitation, we propose a style replay method that leverages the characteristics of low-level representations in CNN to require only one style feature for each domain, leading to a significant reduction in storage overhead. By fusing the style features of past domains with the semantic features of current data, our method enables style transfer for new domain data, thereby improving the model’s generalization ability to the domain. Through extensive experimental evaluations on various autonomous driving datasets, we demonstrate the efficacy of our proposed method in addressing the challenges of continual semantic segmentation under both label and domain shift, outperforming the previous state-of-the-art methods. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.

关键词： Semantic Segmentation

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A Robust CPD Approach Based on Shape Context 33

A Robust CPD Approach Based on Shape Context

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第三十三届中国控制会议

作者： YANG Yawen ZHANG Peng Peng QIAO Yu YANG Jie WANG Sheng Zheng The Key Laboratory of Ministry of Education for System Control and Information Processing Institute of Image Processing and Pattern RecognitionShanghai Jiao Tong University Merchant Marine College Shanghai Maritime University

ISBN: (纸本)9781479947249

Point matching is an important component of image *** years,Coherent Point Drift(CPD) method becomes a very popular point matching *** treats point matching as a probability estimation problem and speeds up the process of matching a *** this method,one set of points are thought to be sampled from a Gaussian Mixture Model(GMM),which is centered by the other set of ***,CPD is sensitive to outliers and noises,especially when the noise ratio increased or the number of outliers gets much *** deal with this problem,we introduce shape context into the step of searching for matching points and then improve the form of prior probabilities of GMM in this *** main idea of our method is that if the most points in a data set are likely to be matched to a particular centroid,this Gaussian component should be have a more influence to ***,we set prior probability of GMM with the similarity between GMM components and the data *** the computation of similarity is based on shape *** experiments on 2D and 3D images show that when noise ratio is low,our method performs as well as CPD does,but as the ratio increased,our method is more robust and satisfactory than CPD.

关键词： CPD,Shape context,image registration,Point matching

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Parallel generate entry landing footprint using differential evolution

Parallel generate entry landing footprint using differential...

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2012 International Symposium on Computer, Consumer and control, IS3C 2012

作者： Chang, Songtao Wang, Yongji Liu, Lei Dep. of Control Science and Engineering Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan China

ISBN: (纸本)9780769546551

Landing footprint of an entry vehicle provides critical information for mission planning. Conventional methods calculate it through solving a family of multi-constraints optimal control problems. It is difficult to solve. To generate the boundary of it, we proposed a new scheme based on differential evolution(DE). Utilizing DE's parallelism, each run generates one side instead of one point of the boundary. To get the full boundary, just need run the algorithm two times. The algorithm's merits include accurate, fast, not relying on simplification of the system, all control variables are under consideration and facilitating parallel programming. © 2012 IEEE.

关键词： Evolutionary algorithms

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NEAT versus PSO for evolving autonomous multi-agents coordination on pursuit-evasion problem

NEAT versus PSO for evolving autonomous multi-agents coordin...

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2011 3rd International Asia Conference on Informatics in control, Automation and Robotics, CAR 2011

作者： Zhao, Jin Peng, Gang Department of Control Science and Engineering Huazhong University of Science and Technology Wuhan China Key Laboratory of Image Processing and Intelligent Control of Education Ministry China

ISBN: (纸本)9783642259913

A method based on multi-agents and ANN (Artificial Neural Network) was proposed to solve the pursuit-evasion task in continuous time-varying environment. According to this method, several autonomous agents with 8 circular sector sensors and an ANN controller were used to form a coordinated behavior to capture the evaders. To evolve the controller, NEAT (NeuroEvolution of Augmenting Topologies) and PSO (Particle Swarm Optimization) method were used to optimize the network. The simulation experiments show that both methods can successfully evolve the controller to capture the evaders, while NEAT requires less swarm members and consume less time comparing to PSO method. © 2011 Springer-Verlag.

关键词： controllers

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H∞ based decoupling tracking control of hypersonic vehicle 19

H∞ based decoupling tracking control of hypersonic vehicle

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19th IFAC World Congress on International Federation of Automatic control, IFAC 2014

作者： Lan, Xuejing Wang, Zhishen Liu, Lei Wang, Yongji School of Automation Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Huazhong University of Science and Technology Wuhan430074 China

ISBN: (纸本)9783902823625

The H∞ based decoupling tracking control is studied in this paper. A virtual system constituted by the controlled system and the no coupling reference model is firstly set up. The controlled system is driven to follow the reference model to realize the decoupling. And the tracking error can be formulated by the H∞ norm of the virtual system. Then the controller is derived by minimizing the H∞ norm, which can be described by Linear Matrix Inequalities (LMIs). The necessary and sufficient condition of existence of controller is derived based on the LMIs above. A flight control example is given to illustrate the effectiveness of the proposed method. The simulation results show that the proposed method is of better control performance than Linear Quadratic (LQ) tracking controller. © IFAC.

关键词： controllers

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