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A protocol for structured illumination microscopy with minimal reconstruction artifacts

Biophysics Reports 2019年第2期5卷 80-90页

作者： Junchao Fan Xiaoshuai Huang Liuju Li Shan Tan Liangyi Chen Key Laboratory of Image Processing and Intelligent Control of Ministry of Education of China School of Automation Huazhong University of Science and Technology State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular Medicine Peking University

The imaging rate of structured illumination microscopy(SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a result, the signal-to-noise ratio(SNR) decreases sharply. Furthermore, the SNR decreases further because of photobleaching and phototoxicity. This decreased quality of SIM raw data may lead to surprising artifacts with various causes, which may confuse a new user of SIM microscopy. We summarize three significant possible sources of severe artifacts in reconstructed super-resolution(SR)images. Ultrafast motion of a biological sample or an uneven illumination pattern is the most difficult to be identified. The estimated parameter could also be incorrect, leading to artifact of regular ***, reconstruction with the Wiener method generates stochastic artifacts due to the amplification of noise during the deconvolution process. To deal with these problems, we have established a protocol to reconstruct ultrafast SIM raw data obtained in low SNR conditions. First, we checked the quality of the raw data with the image J plugin SIMcheck before reconstruction. Then, a modified parameter estimation method was used to improve the precision of the parameters. Finally, an iterative algorithm was used for SIM reconstruction under low signal-to-noise ratio conditions. This procedure effectively suppressed the artifacts in the super-resolution images reconstructed from raw data of low signal-to-noise ratio.

关键词： Ultrafast SIM Low signal-to-noise ratio Reconstruction

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PUGAN: Physical Model-Guided Underwater image Enhancement Using GAN with Dual-Discriminators

arXiv

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arXiv 2023年

作者： Cong, Runmin Yang, Wenyu Zhang, Wei Li, Chongyi Guo, Chun-Le Huang, Qingming Kwong, Sam Institute of Information Science Beijing Jiaotong University Beijing100044 China School of Control Science and Engineering Shandong University Jinan250061 China Key Laboratory of Machine Intelligence and System Control Ministry of Education Jinan250061 China Beijing Key Laboratory of Advanced Information Science and Network Technology Beijing100044 China College of Computer Science Nankai University Tianjin300350 China School of Computer Science and Technology University of Chinese Academy of Sciences Beijing101408 China Key Laboratory of Intelligent Information Processing Institute of Computing Technology Chinese Academy of Sciences Beijing100190 China Peng Cheng Laboratory Shenzhen518055 China Department of Computer Science City University of Hong Kong Hong Kong City University of Hong Kong Shenzhen Research Institute Shenzhen51800 China

Due to the light absorption and scattering induced by the water medium, underwater images usually suffer from some degradation problems, such as low contrast, color distortion, and blurring details, which aggravate the difficulty of downstream underwater understanding tasks. Therefore, how to obtain clear and visually pleasant images has become a common concern of people, and the task of underwater image enhancement (UIE) has also emerged as the times require. Among existing UIE methods, Generative Adversarial Networks (GANs) based methods perform well in visual aesthetics, while the physical model-based methods have better scene adaptability. Inheriting the advantages of the above two types of models, we propose a physical model-guided GAN model for UIE in this paper, referred to as PUGAN. The entire network is under the GAN architecture. On the one hand, we design a Parameters Estimation subnetwork (Par-subnet) to learn the parameters for physical model inversion, and use the generated color enhancement image as auxiliary information for the Two-Stream Interaction Enhancement subnetwork (TSIE-subnet). Meanwhile, we design a Degradation Quantization (DQ) module in TSIE-subnet to quantize scene degradation, thereby achieving reinforcing enhancement of key regions. On the other hand, we design the Dual-Discriminators for the style-content adversarial constraint, promoting the authenticity and visual aesthetics of the results. Extensive experiments on three benchmark datasets demonstrate that our PUGAN outperforms state-of-the-art methods in both qualitative and quantitative metrics. The code and results can be found from the link of https://***/proj_***. © 2023, CC BY-NC-SA.

关键词： Discriminators

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Enabling Stateful TCP Performance Profiling with key Event Capturing

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IEEE Transactions on Network and Service Management 2025年

作者： Geng, Ruopeng Lu, Jianyuan Fang, Chongrong Zhang, Shaokai Zhao, Jiangu Zong, Zhigang Lyu, Biao Zhu, Shunmin Cheng, Peng Chen, Jiming Zhejiang University State Key Laboratory of Industrial Control Technology Hangzhou310027 China Alibaba Group Hangzhou310052 China Ministry of Education of China Key Laboratory of System Control and Information Processing China Shanghai Jiao Tong University Shanghai Engineering Research Center of Intelligent Control and Management Department of Automation Shanghai China Zhejiang University College of Control Science and Engineering Hangzhou310027 China

TCP ensures reliable transmission through its stateful implementation and remains crucial today. TCP performance profiling is essential for tasks like diagnosing network performance problems, optimizing transmission performance, and developing new TCP variants, etc. Existing profiling methods lack enough attention to TCP state transition to provide detailed insights on TCP performance. Thus, we build TcpSight, a tool focusing on TCP state transition throughout connection lifetimes. TcpSight conducts stateful analysis by capturing key events using an efficient per-connection lock-free data management mechanism. Besides, TcpSight enhances profiling by integrating application layer information collected from the TCP stack. With the profiling results, users can identify the culprit of TCP performance degradation, and evaluate the performance of TCP algorithms. We design optional modules and filtering mechanisms to reduce TcpSights overhead. Our evaluation presents that TcpSight incurs an additional CPU consumption of about 16.6% (without filtering) and 10.6% (with filtering) when the server's load is 55.7%, and generates storage consumption about 1.88 KB per connection on average. We also give application cases of TcpSight and the deployment experiences in Alibaba Cloud. TcpSight helps in revealing meaningful findings and insights into exploiting TCP in the production deployment. © 2004-2012 IEEE.

关键词： Information management

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3D Hierarchical Refinement and Augmentation for Unsupervised Learning of Depth and Pose from Monocular Video

arXiv

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arXiv 2021年

作者： Wang, Guangming Zhong, Jiquan Zhao, Shijie Wu, Wenhua Liu, Zhe Wang, Hesheng Department of Automation Institute of Medical Robotics Key Laboratory of System Control and Information Processing Ministry of Education Key Laboratory of Marine Intelligent Equipment and System of Ministry of Education Shanghai Engineering Research Center of Intelligent Control and Management Shanghai Jiao Tong University Shanghai200240 China Department of Engineering Mechanics Shanghai Jiao Tong University Shanghai200240 China Department of Computer Science and Technology University of Cambridge CambridgeCB2 1TN United Kingdom

Depth and ego-motion estimations are essential for the localization and navigation of autonomous robots and autonomous driving. Recent studies make it possible to learn the per-pixel depth and ego-motion from the unlabeled monocular video. A novel unsupervised training framework is proposed with 3D hierarchical refinement and augmentation using explicit 3D geometry. In this framework, the depth and pose estimations are hierarchically and mutually coupled to refine the estimated pose layer by layer. The intermediate view image is proposed and synthesized by warping the pixels in an image with the estimated depth and coarse pose. Then, the residual pose transformation can be estimated from the new view image and the image of the adjacent frame to refine the coarse pose. The iterative refinement is implemented in a differentiable manner in this paper, making the whole framework optimized uniformly. Meanwhile, a new image augmentation method is proposed for the pose estimation by synthesizing a new view image, which creatively augments the pose in 3D space but gets a new augmented 2D image. The experiments on KITTI demonstrate that our depth estimation achieves state-of-the-art performance and even surpasses recent approaches that utilize other auxiliary tasks. Our visual odometry outperforms all recent unsupervised monocular learning-based methods and achieves competitive performance to the geometry-based method, ORB-SLAM2 with back-end optimization. Copyright © 2021, The Authors. All rights reserved.

关键词： Robots

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Multi-Party dual learning

arXiv

引用

arXiv 2021年

作者： Gong, Maoguo Gao, Yuan Xie, Yu Qin, A.K. Pan, Ke Ong, Yew-Soon The School of Electronic Engineering Key Laboratory of Intelligent Perception and Image Understanding Ministry of Education Xidian University Xi'an Shaanxi Province710071 China The Key Laboratory of Computational Intelligence and Chinese Information Processing Ministry of Education Shanxi University Taiyuan030006 China The Department of Computer Science and Software Engineering Swinburne University of Technology Melbourne Australia The School of Computer Science and Engineering Nanyang Technological University Singapore639798 Singapore

The performance of machine learning algorithms heavily relies on the availability of a large amount of training data. However, in reality, data usually reside in distributed parties such as different institutions and may not be directly gathered and integrated due to various data policy constraints. As a result, some parties may suffer from insufficient data available for training machine learning models. In this paper, we propose a multi-party dual learning (MPDL) framework to alleviate the problem of limited data with poor quality in an isolated party. Since the knowledge sharing processes for multiple parties always emerge in dual forms, we show that dual learning is naturally suitable to handle the challenge of missing data, and explicitly exploits the probabilistic correlation and structural relationship between dual tasks to regularize the training process. We introduce a feature-oriented differential privacy with mathematical proof, in order to avoid possible privacy leakage of raw features in the dual inference process. The approach requires minimal modifications to the existing multi-party learning structure, and each party can build flexible and powerful models separately, whose accuracy is no less than non-distributed self-learning approaches. The MPDL framework achieves significant improvement compared with state-of-the-art multi-party learning methods, as we demonstrated through simulations on real-world datasets. Copyright © 2021, The Authors. All rights reserved.

关键词： Machine learning

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Industrial Scene Text Detection with Refined Feature-attentive Network

arXiv

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arXiv 2021年

作者： Guan, Tongkun Gu, Chaochen Lu, Changsheng Tu, Jingzheng Feng, Qi Wu, Kaijie Guan, Xinping The Department of Automation Shanghai Jiao Tong University Key Laboratory of System Control and Information Processing Ministry of Education of China Shanghai Engineering Research Center of Intelligent Control and Management Shanghai200240 China The College of Engineering and Computer Science The Australian National University CanberraACT2600 Australia

Detecting the marking characters of industrial metal parts remains challenging due to low visual contrast, uneven illumination, corroded surfaces, and cluttered background of metal part images. Affected by these factors, bounding boxes generated by most existing methods could not locate low-contrast text areas very well. In this paper, we propose a refined feature-attentive network (RFN) to solve the inaccurate localization problem. Specifically, we first design a parallel feature integration mechanism to construct an adaptive feature representation from multi-resolution features, which enhances the perception of multi-scale texts at each scale-specific level to generate a high-quality attention map. Then, an attentive proposal refinement module is developed by the attention map to rectify the location deviation of candidate boxes. Besides, a re-scoring mechanism is designed to select text boxes with the best rectified location. To promote the research towards industrial scene text detection, we contribute two industrial scene text datasets, including a total of 102156 images and 1948809 text instances with various character structures and metal parts. Extensive experiments on our dataset and four public datasets demonstrate that our proposed method achieves the state-of-the-art performance. Both code and dataset are available at: https://***/TongkunGuan/RFN. © 2021, CC BY.

关键词： Character recognition

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FCM-RDpA: TSK fuzzy regression model construction using fuzzy C-means clustering, regularization, droprule, and powerball adabelief

arXiv

引用

arXiv 2020年

作者： Shi, Zhenhua Wu, Dongrui Guo, Chenfeng Zhao, Changming Cui, Yuqi Wang, Fei-Yue Ministry of Education Key Laboratory of Image Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan China State Key Laboratory for Management and Control of Complex Systems Institute of Automation Chinese Academy of Sciences Beijing China

To effectively optimize Takagi-Sugeno-Kang (TSK) fuzzy systems for regression problems, a mini-batch gradient descent with regularization, DropRule, and AdaBound (MBGD-RDA) algorithm was recently proposed. This paper further proposes FCM-RDpA, which improves MBGD-RDA by replacing the grid partition approach in rule initialization by fuzzy c-means clustering, and AdaBound by Powerball AdaBelief, which integrates recently proposed Powerball gradient and AdaBelief to further expedite and stabilize parameter optimization. Extensive experiments on 22 regression datasets with various sizes and dimensionalities validated the superiority of FCM-RDpA over MBGD-RDA, especially when the feature dimensionality is higher. We also propose an additional approach, FCM-RDpAx, that further improves FCM-RDpA by using augmented features in both the antecedents and consequents of the rules. Copyright © 2020, The Authors. All rights reserved.

关键词： Gradient methods

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CNN-Transformer fusion for improving maize kernel storage Quality: A new approach to reducing storage risks

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Journal of Stored Products Research 2025年 114卷

作者： Zhao, Like Jia, Weishi Tao, Mengting Jiang, Huawei Yang, Zhen Liu, Xixi Key Laboratory of Grain Information Processing and Control (Henan University of Technology) Ministry of Education Zhengzhou 450001 China Henan Key Laboratory of Grain Storage Information Intelligent Perception and Decision Making Henan University of Technology Zhengzhou 450001 China College of Information Science and Engineering Henan University of Technology Zhengzhou 450001 China

The efficient identification of imperfect maize kernels is of crucial importance for optimizing storage management and ensuring quality preservation. image-based technologies, known for their rapid and non-destructive characteristics, play a pivotal role in this endeavor. However, these technologies encounter significant challenges due to high inter-class similarity and substantial intra-class variability among maize kernels. To address these challenges, we propose CTNet, an advanced model that synergistically integrates the Convolutional Neural Network (CNN) with the Transformer architecture. This integration is further enhanced by incorporating a Feature Attention Module (FAM) and a DW-Swin Transformer, which collectively facilitate the fusion of local and global features. A Fine-Grained Perception Module (FGPM) is employed to augment the model's sensitivity to subtle imperfections. The model, supplemented with a linear classifier, ensures precise discrimination of kernel quality. CTNet was trained on the comprehensive GrainSpace dataset and achieved 1.35 % higher accuracy than the baseline model, SwinTransformer, on the test set. Compared with other methods, our model demonstrates higher accuracy in identifying defective maize grains and achieves more efficient parameter utilization in its structure. Despite moderate parameters and FLOPs, it enhances recognition performance while maintaining low complexity. As imperfect maize grains directly impact quality and storage safety, the model's effective recognition capability is critical for reducing storage losses and ensuring safety, thus providing strong support for smart agriculture. © 2025 Elsevier Ltd

关键词： CNN Fine-grained perception Maize imperfect kernel identification Swin transformer

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A polynomial chaos approach to robust H∞ static output-feedback control with bounded truncation error

arXiv

引用

arXiv 2021年

作者： Wan, Yiming Shen, Dongying E. Lucia, Sergio Findeisen, Rolf Braatz, Richard D. Artificial Intelligence and Automation Huazhong University of Science and Technology Key Laboratory of Image Processing and Intelligent Control Ministry of Education Wuhan430074 China Massachusetts Institute of Technology 77 Massachusetts Avenue CambridgeMA02139 United States TU Dortmund University Dortmund44227 Germany Otto-von-Guericke University Magdeburg Magdeburg39106 Germany

This article considers the H∞ static output-feedback control for linear time-invariant uncertain systems with polynomial dependence on probabilistic time-invariant parametric uncertainties. By applying polynomial chaos theory, the control synthesis problem is solved using a high-dimensional expanded system which characterizes stochastic state uncertainty propagation. A closed-loop polynomial chaos transformation is proposed to derive the closed-loop expanded system. The approach explicitly accounts for the closed-loop dynamics and preserves the L2-induced gain, which results in smaller transformation errors compared to existing polynomial chaos transformations. The effect of using finite-degree polynomial chaos expansions is first captured by a norm-bounded linear differential inclusion, and then addressed by formulating a robust polynomial chaos based control synthesis problem. This proposed approach avoids the use of high-degree polynomial chaos expansions to alleviate the destabilizing effect of truncation errors, which significantly reduces computational complexity. In addition, some analysis is given for the condition under which the robustly stabilized expanded system implies the robust stability of the original system. A numerical example illustrates the effectiveness of the proposed approach. © 2021, CC BY.

关键词： Feedback control

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Multi-scale feature fusion distilled attention network for efficient image super-resolution

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Applied Soft Computing 2025年 180卷

作者： Yinggan Tang Mengjie Su Xiuli Zhang School of Electrical Engineering Yanshan University Qinhuangdao Hebei 066004 China Key Laboratory of Intelligent Rehabilitation and Neromodulation of Hebei Province Yanshan University Qinhuangdao Hebei 066004 China Key Laboratory of Intelligent Control and Neural Information Processing Ministry of Education Yanshan University Qinhuangdao Hebei 066004 China School of Hebei Vocational and Technical College of Building Materials Qinhuangdao 066004 China

Efficient single image super-resolution (SISR) seeks to enhance reconstruction quality while minimizing computational complexity and model parameters for practical deployment on resource-constrained edge devices. A critical limitation of existing approaches lies in the restricted receptive fields of conventional convolutions, which hinder attention mechanisms and backbone networks from effectively capturing non-local features, ultimately degrading super-resolution performance. To solve this challenge, we propose a novel Multi-scale Feature Fusion Distilled Attention Network (MFFDAN) that synergizes multi-scale feature extraction with lightweight attention mechanisms. Our framework introduces three key innovations. First, we proposed a Multi-scale Convolution (MConv) module that dynamically extracts hierarchical features with multi-scale receptive fields while maintaining parameter efficiency, subsequently extended into a Multi-scale Feature Fusion Distillation Block (MFFDB). Second, we proposed a Distilled Spatial Attention (DSA) mechanism that enhances spatial feature interactions through multi-scale downsampling and distillation-based information refinement. Third, a lightweight Depth-wise Channel Attention (DCA) module optimized via depth-wise convolutions for efficient channel feature selection is proposed. These components are systematically integrated through residual connections to form the Multi-scale Feature Fusion Distilled Attention Block (MFFDAB), the core building block of our architecture. Extensive experiments demonstrate that MFFDAN achieves state-of-the-art performance while maintaining superior computational efficiency, achieving an optimal balance between reconstruction quality (PSNR/SSIM), model complexity (parameters/Multi-Adds), and inference speed across benchmark datasets. The proposed innovations provide a practical solution for deploying high-quality SISR models on edge computing platforms.

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